Method for improving in-vivo and in-vitro antioxidant activity of pomegranate peel polyphenol extract through combined enzymolysis of tannase and beta-glucosidase

Through the combination of tannins and β-glucosidase and enzymatic pomegranate polyphenols, the problem of inability to absorb macromolecule ellagitannins is solved, the bioavailability and antioxidant activity of pomegranate polyphenols is improved, and the oxidative loss in oxidative stress mice is improved.

CN120392837APending Publication Date: 2025-08-01NANCHANG UNIV
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Patent Information

Application Number
CN202510636600.5
Authority / Receiving Office
CN · China
Patent Type
Applications(China)
Current Assignee / Owner
Filing Date
2025-05-17
Publication Date
2025-08-01

AI Technical Summary

Technical Problem

In the prior art, the bioavailability and antioxidant activity of pomegranate polyphenol extracts are limited, especially the macromolecule ellagia tannins cannot be directly absorbed by the animal intestines, and require complex enzymatic decomposition, but the effect is average.

Method used

Tannins and β-glucosidase combined with enzymatic pomegranate polyphenols were used to hydrolyze macromolecule ellagicans into small-molecular substances such as ellagic acid and flavonoid aglycones to improve their biological activity.

Benefits of technology

The antioxidant activity of pomegranate polyphenol extract in vivo was significantly improved. After combined enzymatic lysis, the content of macromolecules ellagia tannins decreased, and the content of small molecules ellagia and gallic acid increased, improving the oxidation loss in oxidative stress mice.

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Abstract

The invention discloses a method for improving in-vivo and in-vitro antioxidant activity of pomegranate peel polyphenol extract through combined enzymolysis of tannase and beta-glucosidase, and relates to the technical field of food science and biology. The pomegranate peel polyphenol extract provided by the invention is obtained from pomegranate peel powder through solvent extraction. According to the present invention, tannase and beta-glucosidase are adopted to perform single or combined enzymolysis on the pomegranate peel polyphenol extract, and the determination on the in vitro antioxidant activity of the pomegranate peel polyphenol extract shows that the antioxidant activities of DPPH and ABTS after the combined enzymolysis are improved by 29.84% and 29.30% compared with the antioxidant activities of the untreated polyphenol extract; the UPLC-ESI-QTOF-MS is used for identifying and analyzing the components of the enzymatic hydrolysate, the content of macromolecular ellagic tannin is obviously reduced, and the content of micromolecular ellagic acid and gallic acid is increased. The enzymolysis polyphenol is applied to D-galactose induced oxidative stress mice, and the result shows that the oxidative damage relieving effect of the enzymolysis polyphenol on the mice is better than that of untreated polyphenol.
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