Marine bacterium Bacillus sp.SQ3, method for producing dextranase by using marine bacterium Bacillus sp.SQ3 and application of marine bacterium Bacillus sp.SQ3

Through the marine bacteria Bacillus sp.SQ3 and its dextranase-producing method, the problem of efficient preparation of high-polymerization oligosaccharides in the prior art is solved, and the efficient preparation of high-polymerization isomalt oligosaccharides is achieved, which improves the functional effect of prebiotics.

CN120442449AActive Publication Date: 2025-08-08JIANGSU OCEAN UNIV
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Patent Information

Application Number
CN202510487910.5
Authority / Receiving Office
CN · China
Patent Type
Applications(China)
Current Assignee / Owner
Filing Date
2025-04-18
Publication Date
2025-08-08
Estimated Expiration
2045-04-18

AI Technical Summary

Technical Problem

The existing oligosaccharide production and processing technology lacks key enzymes to efficiently prepare high-polymerization oligosaccharides, resulting in insufficient proportion of high-polymerization oligosaccharides in prebiotic products, weakening its effect in promoting the growth of probiotics.

Method used

A marine bacteria Bacillus sp.SQ3 and its dextranase are provided. Cultivating the strain through specific culture medium and conditions, the obtained dextranase can efficiently hydrolyze dextran to prepare high-polymerization isomalt oligosaccharides, and the proportion of high-polymerization oligosaccharides in the product reaches 92%.

Benefits of technology

It has achieved efficient preparation of high-polymerization oligosaccharides, significantly improved the functional effect of prebiotics, promoted the growth of intestinal probiotics and the balance of intestinal bacteria, and enhanced the function of intestinal barriers and the body's immunity.

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Abstract

The invention relates to the technical field of microorganisms, and particularly discloses a marine bacterium Bacillus sp.SQ3 and a method for producing dextranase and application thereof, the strain is preserved in China General Microbiological Culture Collection Center (CGMCC) on January 20, 2025, the preservation number is CGMCC No.33458, the preservation unit address is No.3, Yard 1, Beichen West Road, Chaoyang District, Beijing, and the preservation number is CGMCC No.33458. Chinese Academy of Sciences Institute of Microbiology. The dextranase produced by the strain SQ3 has the advantage of preparing high-polymerization-degree isomaltooligosaccharides, in hydrolysate of the dextranase, the ratio of tetrasaccharide is 9.9%, the ratio of pentasaccharide is 15.5%, the ratio of hexasaccharide is 32.7%, the ratio of heptasaccharide is 33.9%, the total ratio reaches 92%, and the strain and the enzyme produced by the strain have the remarkable advantage of being applied to preparing the high-polymerization-degree oligosaccharides.
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