Application and method of oself3-2 gene in controlling heading date of rice

By overexpressing the OsELF3-2 gene in rice and using Agrobacterium-mediated genetic transformation, the problem of controlling the heading period of rice was solved, resulting in delayed heading, increased plant height, enhanced dry matter accumulation, and improved crop yield.

CN120464679BActive Publication Date: 2026-07-24CHINA NAT RICE RES INST
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Patent Information

Application Number
CN202510706335.3
Authority / Receiving Office
CN · China
Patent Type
Patents(China)
Current Assignee / Owner
Filing Date
2025-05-29
Publication Date
2026-07-24
Estimated Expiration
2045-05-29

AI Technical Summary

Technical Problem

Existing technologies make it difficult to precisely control the heading stage of rice, which makes it difficult to balance the dry matter accumulation and yield, thus affecting crop yield.

Method used

Overexpression of the OsELF3-2 gene in rice was achieved by constructing the OsELF3-2 gene into a vector through Agrobacterium-mediated genetic transformation. Positive transgenic rice plants were screened, and the OsELF3-2 gene inhibited rice heading under both short-day and long-day conditions.

Benefits of technology

Delaying the heading date of rice resulted in a significantly late heading phenotype, increased plant height, and expression of the OsELF3-2 gene in various tissues. By promoting the expression of Ghd7 and inhibiting Ehd1, the OsELF3-2 gene enriched the photoperiodic flowering regulatory network of rice.

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Abstract

The application discloses application and application method of an OsELF3-2 gene in controlling rice heading stage, the gene OsELF3-2 is from a rice variety Nipponbare, and the cDNA sequence is shown as sequence 1; genetic transformation experiments show that overexpression of the OsELF3-2 gene inhibits rice heading under short-day and long-day conditions; the application finds that the OsELF3-2 gene is expressed in a diurnal rhythm, promotes expression of Ghd7 and OsGI, further inhibits transcription level of Ehd1, finally down-regulates expression of Hd3a / RFT1, and causes heading delay.
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