Oral care composition with effects of inhibiting bacteria and preventing senile dementia

Abstract

The invention discloses an oral care composition with antibacterial and senile dementia prevention effects, which comprises the following raw materials: berberis poiretii schneid extract and folium eriobotryae extract, and the dosage ratio of the berberis poiretii schneid extract to the folium eriobotryae extract is (10: 1)-(1: 10). The berberis poiretii schneid extract and the folium eriobotryae extract in a proper proportion are selected to be compounded and combined, it is found that the obtained composition has the effect of preventing senile dementia and also has the effects of synergistic interaction, bacteriostasis and oral ulcer prevention and treatment, and the bacteriostatic effect is far better than that of single berberis poiretii schneid extract; on the basis, a series of oral care products with marketization potential are developed, and the oral care products are safe and free of side effects, so that middle-aged and elderly people can clean gingiva, prevent and treat oral ulcer and prevent or delay senile dementia in daily oral care behaviors such as tooth brushing and mouth rinsing, and the oral care products are safe and free of side effects. The product is an excellent daily necessity beneficial to the public for early prevention of senile dementia, and has a considerable application prospect.

Description

Technical Field

[0001] The present invention relates to the fields of daily chemical products and oral hygiene products, and in particular to an oral care composition having both antibacterial and Alzheimer's prevention effects. Background Art

[0002] As people's living standards improve, oral health is receiving more and more attention. Bad breath has also become a problem that troubles many people and can easily cause embarrassment in normal social interactions. The key to oral health is to prevent the growth of bacteria in the mouth. Commonly used toothpastes can keep breath fresh for a period of time, but most of them solve the breath problem from a sensory perspective by using cooling ingredients such as mint and flavors to achieve short-term freshness, or add substances that can combine with odorous gas components in the mouth to remove odorous gases, or enhance the cleaning effect of surfactants to remove odorous substances produced by harmful bacteria in the mouth. However, these do not solve the problem from the root, that is, to remove harmful bacteria in the mouth. Most of the antibacterial toothpastes currently available on the market contain chemically synthesized antibacterial agents. Although they can have a certain antibacterial effect, oral flora can easily develop resistance and dependence on these antibacterial ingredients, which is not conducive to the prevention and treatment of oral diseases, and the toxic side effects on the human body need to be considered.

[0003] Oral ulcers are small, shallow lesions that appear inside the mouth or at the base of the gums. While most canker sores resolve on their own within a week or two, they can cause pain and make eating and speaking difficult, disrupting daily life. Most toothpastes on the market have antibacterial properties and may help prevent oral ulcers, but the effectiveness is limited.

[0004] Currently, plant extracts are widely used in toothpaste, but most of them are combinations of a large number of plant extracts. The production cost and process difficulty are relatively high, and the added amount is usually small, so the effect needs to be considered.

[0005] With the increasing aging of the population, the prevalence of Alzheimer's disease (also known as dementia) continues to rise. However, since the pathogenesis and causes of Alzheimer's disease remain unclear and there is currently no effective cure, prevention is crucial. The pathological hallmark of Alzheimer's disease is the damage or loss of pathological neuronal groups, but there is currently no toothpaste that can prevent Alzheimer's disease.

[0006] Based on the above market status, developing oral cleaning products that can remove harmful oral bacteria, have a certain heat-clearing and detoxifying effect, and prevent Alzheimer's disease is the current technical pain point.

[0007] The applicant has found that most existing oral cleaning products only have antibacterial effects or prevent and treat oral ulcers, and few products can also prevent Alzheimer's disease. The relevant literature on antibacterial toothpaste is as follows: For example, patent CN115025022A discloses a highly effective antibacterial toothpaste containing sea fennel extract. The toothpaste components include 1-6% sea fennel extract and 0.01-0.1% O-cymene-5-ol. This technical solution reduces the impact of high-content sea fennel extract on the toothpaste formula by adding a small amount of O-cymene-5-ol, and improves the antibacterial effect of the sea fennel extract.

[0008] For example, patent CN108635283A discloses a compound antibacterial composition containing apocynum venetum leaf extract and sea buckthorn oil extract. Adding the antibacterial composition to toothpaste can play an oral health care role in preventing and protecting oral diseases.

[0009] For example, patent CN112569154A discloses an oral care product with a plant compound antibacterial composition as an active ingredient, wherein the plant compound antibacterial composition is composed of peony bark extract, lianmianzhen extract, licorice extract, kudzu root extract, honeysuckle extract, dangxueliu extract, Panax notoginseng extract, and Cimicifuga extract.

[0010] For example, patent CN113679632A discloses the use of the antimicrobial peptide compound DM80Bu20 in the preparation of antibacterial toothpaste and its antibacterial toothpaste. The antibacterial toothpaste is prepared by compounding the antimicrobial peptide compound DM80Bu20 with other effective ingredients (lysozyme, lactoferrin, probiotics, regenerated silicon, and oligofructose). The ingredients synergistically inhibit harmful oral bacteria, overcoming the shortcoming of traditional Chinese herbal toothpaste that has no selectivity in inhibiting a variety of oral pathogens and probiotics.

[0011] In summary, the active ingredients in existing oral care products, whether small molecule chemical drugs, peptide biopharmaceuticals, or plant extracts, have been developed solely for the purpose of inhibiting harmful oral bacteria. No toothpaste products have yet been developed that can simultaneously inhibit bacteria, prevent and treat oral ulcers, and prevent Alzheimer's disease. Therefore, developing an oral care composition that can simultaneously inhibit bacteria, treat oral ulcers, and prevent Alzheimer's disease remains a technical challenge for those skilled in the art. To address these issues, the following proposes a solution. Summary of the Invention

[0012] The present invention aims to provide an oral care composition having both antibacterial and Alzheimer's prevention effects, which has the advantages of antibacterial, prevention and treatment of oral ulcers and prevention of Alzheimer's disease.

[0013] The above technical objectives of the present invention are achieved through the following technical solutions: Disclosed is an oral care composition having both antibacterial and Alzheimer's prevention effects, comprising the following raw materials: a Tripterygium wilfordii extract, an loquat leaf extract, and excipients, wherein the mass ratio of the Tripterygium wilfordii extract to the loquat leaf extract is 10:1 to 1:10, and the amount of the Tripterygium wilfordii extract is 0.1-10 parts by mass, and the amount of the loquat leaf extract is 0.01-1 part by mass; the excipients include flavoring agents and preservatives.

[0014] Preferably, the amount of the Tripterygium wilfordii extract is 10 parts, and the amount of the loquat leaf extract is 1 part.

[0015] Preferably, the flavoring agent comprises one or more of vanilla extract, mint oil, and citrus oil.

[0016] Preferably, the preservative includes one or more of sodium benzoate, potassium sorbate, and parahydroxybenzoate.

[0017] Preferably, the oral care composition is a liquid preparation for oral use or a semisolid preparation for oral use.

[0018] Preferably, when the oral care composition is a liquid preparation for oral use, it is selected from a dosage form of solution, lotion, and spray; when the oral care composition is a semisolid preparation for oral use, it is selected from a dosage form of ointment, cream, and gel.

[0019] The invention discloses an oral care composition having both antibacterial and Alzheimer's prevention effects and its use in the preparation of drugs, cosmetics or daily necessities for preventing or delaying Alzheimer's disease.

[0020] Preferably, the oral care composition is in the form of an oral spray, and the components in the oral spray and their amounts by mass include the following: 0.1-10% of Tripterygium wilfordii extract, 0.01-1% of loquat leaf extract, 0.1-1% of vitamin C, 0.01-1% of lactitol, 0.01-0.5% of menthol, 0.5-5% of ethanol, and the balance being water.

[0021] The beneficial effects of the present invention are as follows: by selecting an appropriate ratio of Tripterygium wilfordii extract and loquat leaf extract for compounding, the present invention found that the obtained composition not only has the effect of preventing Alzheimer's disease, but also has synergistic antibacterial effects, as well as the efficacy of preventing and treating oral ulcers. The antibacterial effect and the effect of preventing and treating oral ulcers are far superior to those of using Tripterygium wilfordii extract alone; based on this, a series of oral care products with market potential have been developed, which are safe and have no side effects, so that middle-aged and elderly people can not only clean their gums, prevent and treat oral ulcers, but also prevent or delay the occurrence of Alzheimer's disease during daily oral care activities such as brushing and rinsing their mouths. It can be said to be an excellent daily necessity for the early prevention of Alzheimer's disease that benefits the general public and has considerable application prospects. DETAILED DESCRIPTION

[0022] In order to enable those skilled in the art to better understand the present invention, the technical solutions in the present embodiment will be described in detail below. It must be noted that the following embodiments are only used to explain the present invention and are not intended to limit the invention. Any modifications and changes made to the present invention within the spirit of the present invention and the scope of protection of the claims fall within the scope of protection of the present invention.

[0023] Example 1 Preparation of loquat leaf extract The oral care composition provided by the present invention, one of the raw materials of which is loquat leaf extract, is prepared by the following steps: Take the ground loquat leaf powder, add 30-90% ethanol with a volume fraction equivalent to 5-10 times the amount of loquat leaf powder, heat and reflux extraction at 70-90°C for 1-3 times, each time for 1-3 hours, combine the filtrates, concentrate under reduced pressure, and freeze-dry to obtain the loquat leaf extract.

[0024] Example 2 Preparation of Tripterygium wilfordii Extract The oral care composition provided by the present invention, one of the raw materials of which is the extract of Tripterygium wilfordii, is prepared by the following steps: Take the ground three-needle medicinal material powder, take the three-needle medicinal material powder as 1 equivalent, add 60% ethanol solution with a volume fraction at a material-liquid ratio of 1:10, reflux in a 95°C water bath 3 times, each time for 2 hours, filter, and obtain the three-needle extract.

[0025] Example 3 Preparation of a compound containing loquat leaf extract and three needle extract In the oral care composition provided by the present invention, the steps of preparing the compound containing loquat leaf extract and trifoliate needle extract are as follows: The loquat leaf extract prepared in Example 1 and the three needle extract prepared in Example 2 were dissolved in anhydrous ethanol at a ratio of 1:10, stirred at 40°C for 1.5 hours, and the reaction mixture was placed on a rotary evaporator using a solvent-reduced pressure drying method. The reaction solvent was removed by reduced pressure evaporation at 50°C, and then vacuum dried overnight, crushed, and sieved to obtain a compound.

[0026] Example 4 Antibacterial Effect Test of Loquat Leaf Extract and Loquat Leaf Extract 1. Test method: 1) Preparation of bacterial suspension: Staphylococcus aureus, Escherichia coli, Streptococcus mutans, Porphyromonas gingivalis, and Fusobacterium nucleatum were activated and cultured for 48 hours to prepare a test bacterial suspension with a concentration of 1*106 CFU / ml. 2) Culture medium preparation: 10 g peptone, 3 g beef extract, 5 g sodium chloride, 1000 mL distilled water. Mix and dissolve all components, adjust the pH to 7.4, and autoclave at 121°C for 15 min to prepare nutrient agar medium.

[0027] 3) MIC value determination: Loquat leaf extract experimental group: The prepared culture medium was added with 0.2 mg / ml, 0.4 mg / ml, 0.6 mg / ml, 0.8 mg / ml, 1 mg / ml, and 2 mg / ml of loquat leaf extract, respectively, to obtain culture media containing loquat leaf extract at different concentrations. The pH was adjusted to 7.4 with 1 mol / L NaOH, and the plates were sterilized at 121°C for later use. Sterile physiological saline was used instead of loquat leaf extract in the control group.

[0028] For the Tripterygium wilfordii extract experimental group, the prepared culture medium was added with 1 mg / ml, 1.5 mg / ml, 2 mg / ml, 2.5 mg / ml, 3 mg / ml, and 3.5 mg / ml of Tripterygium wilfordii extract. The culture medium containing different concentrations of loquat leaf extract was adjusted to pH 7.4 with 1 mol / L NaOH and sterilized at 121°C to obtain plates for later use. A control group used sterile saline instead of loquat leaf extract.

[0029] Compound experimental group: Select loquat leaf extract and trifoliate needle extract in different proportions, and prepare compounds containing loquat leaf extract and trifoliate needle extract in different proportions according to the method of Example 3. The ratio of loquat leaf extract and trifoliate needle extract can be selected from: 1:5, 1:6, 1:7, 1:8, 1:9, 1:10. Similar to the experimental groups of loquat leaf extract and trifoliate needle extract, take the prepared culture medium and add different concentrations of the compound to obtain culture medium containing different concentrations of the compound, adjust the pH to 7.4 with 1 mol / L NaOH, sterilize at 121°C, obtain plates, and set aside; the control group uses sterile saline instead of loquat leaf extract.

[0030] 4) Result determination: Take 0.1 ml of the test bacterial suspension and inoculate 10 μl of it into 55 partitions of each culture medium plate. Culture at 35-37 ° C for 24-48 hours, observe the growth of the bacteria, and after confirming that there is no growth of contaminating bacteria on all plates, count the colonies with a bacterial colony counter. The concentration that completely inhibits bacterial growth is taken as the minimum inhibitory concentration (MIC) of the extract.

[0031] From the MIC test results, it can be seen that the antibacterial effect of the compound groups with the ratio of loquat leaf extract to three-needle extract of 1:5, 1:6, 1:7, 1:8, 1:9 and 1:10 is better than that of the loquat leaf extract group and the three-needle extract group; and among the same compound groups, the antibacterial effect of the 1:10 compound group is better.

[0032] Example 5 Application examples of the oral care composition provided by the present invention This embodiment provides an application example of an oral care composition having both antibacterial and Alzheimer's disease prevention effects, in the form of an oral spray. Based on a total dosage of 100%, the components and dosages in the oral spray prescription are as follows: 0.1-10% of Tripterygium wilfordii extract, 0.01-1% of loquat leaf extract, 0.1-1% of vitamin C, 0.01-1% of lactitol, 0.01-0.5% of menthol, 0.5-5% of ethanol, and the balance of water. Example 6 Experimental Study on the Treatment of Oral Ulcers with Loquat Leaf Extract and Tripterygium wilfordii Extract According to the formula of Example 5, oral spray is prepared. The components and dosages of experimental group 1 are as follows: Loquat leaf extract 0.6%, vitamin C 0.5%, lactitol 0.1%, menthol 0.5%, ethanol 2%, the balance is water.

[0033] The components and dosages of experimental group 2 are as follows: Three needle extract 2%, vitamin C 0.5%, lactitol 0.1%, menthol 0.5%, ethanol 2%, the balance is water.

[0034] The components and dosages of experimental group 3 are as follows: Loquat leaf extract 0.6%, three needle extract 2%, vitamin C 0.5%, lactitol 0.1%, menthol 0.5%, ethanol 2%, the balance is water.

[0035] Experimental groups 1-3 were used for testing. Twenty volunteers with early-stage oral ulcers and 20 with moderate oral ulcers were divided into groups according to a double-blind study. A control group of 5 participants received a commercially available toothpaste. The oral spray was used three times daily, with no food or water within half an hour of application. Ulcers healed and pain levels were observed for 1-14 consecutive days.

[0036] The specific embodiments described above further illustrate the technical problems, technical solutions and beneficial effects solved by the present invention. It should be understood that the above are only specific embodiments of the present invention and are not intended to limit the present invention. Any modifications, equivalent substitutions, improvements, etc. made within the spirit and principles of the present invention should be included in the scope of protection of the present invention.

Claims

1. An oral care composition having both antibacterial and Alzheimer's prevention effects, characterized in that: The raw materials include the following components: three needle needle extract, loquat leaf extract and auxiliary materials, the mass ratio of the three needle needle extract to the loquat leaf extract is 10:1~1:10, the amount of the three needle needle extract is 0.1-10 parts by mass, and the amount of the loquat leaf extract is 0.01-1 part; the auxiliary materials include flavoring agents and preservatives.

2. The oral care composition having both antibacterial and Alzheimer's prevention effects according to claim 1, characterized in that: In parts by mass, the amount of the three needles extract is 10 parts, and the amount of the loquat leaf extract is 1 part.

3. The oral care composition having both antibacterial and Alzheimer's prevention effects according to claim 1, characterized in that: The flavoring agent includes one or more of vanilla extract, mint oil, and citrus oil.

4. The oral care composition having both antibacterial and Alzheimer's prevention effects according to claim 1, characterized in that: The preservatives include one or more of sodium benzoate, potassium sorbate, and parahydroxybenzoates.

5. The oral care composition having both antibacterial and Alzheimer's prevention effects according to claim 1, characterized in that: The oral care composition is a liquid preparation for oral cavity or a semisolid preparation for oral cavity.

6. The oral care composition having both antibacterial and Alzheimer's prevention effects according to claim 5, characterized in that: When the oral care composition is a liquid preparation for oral use, it is selected from a dosage form of solution, lotion, and spray; when the oral care composition is a semi-solid preparation for oral use, it is selected from a dosage form of ointment, cream, and gel.

7. Use of the oral care composition having both antibacterial and Alzheimer's prevention effects according to any one of claims 1 to 6 in the preparation of drugs, cosmetics or daily necessities for preventing or delaying Alzheimer's disease.

8. The use according to claim 7, characterized in that The oral care composition is in the form of an oral spray. The components in the oral spray and their amounts by mass include: 0.1-10% of Tripterygium wilfordii extract, 0.01-1% of loquat leaf extract, 0.1-1% of vitamin C, 0.01-1% of lactitol, 0.01-0.5% of menthol, 0.5-5% of ethanol, and the balance is water.