Application of PtoERD3 gene structure variation in evaluation of lignin content of poplar

By detecting the 54bp insertion variant SV fragment in the promoter region of the PtoERD3 gene of poplar tree as a molecular marker, the problem of inefficient screening efficiency of poplar wood quality was solved, early accurate screening and efficient breeding were achieved, and the accuracy and efficiency of lignin content regulation were improved.

CN120574979AActive Publication Date: 2025-09-02BEIJING FORESTRY UNIVERSITY
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Patent Information

Application Number
CN202510797710.X
Authority / Receiving Office
CN · China
Patent Type
Applications(China)
Current Assignee / Owner
Filing Date
2025-06-16
Publication Date
2025-09-02
Estimated Expiration
2045-06-16

AI Technical Summary

Technical Problem

The existing technology lacks molecular markers that can quickly and accurately screen excellent wood varieties, resulting in low molecular design and breeding efficiency of aspen wood quality.

Method used

Using a genome-wide association analysis strategy, a 54bp insertion variant SV fragment located in the promoter region of the PtoERD3 gene was detected as a molecular marker. The lignin content in poplar trees was identified by PCR amplification and electrophoresis, and combined with overexpression or silencing of the PtoERD3 gene was regulated.

Benefits of technology

It has achieved accurate and efficient screening of high-quality wood quality traits in the early stages of poplar growth, significantly shortened the breeding cycle, provided a tool for molecular marker assisted breeding, and improved the accuracy and efficiency of lignin content regulation.

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Abstract

The invention discloses an application of PtoERD3 gene structure variation in evaluation of poplar lignin content, the structure variation is located in an upstream promoter region of a poplar PtoERD3 gene, is a 54bp chromosome structure variation SV fragment, and has a nucleotide sequence as shown in SEQ ID NO.1. The invention also discloses an application of the PtoERD3 gene structure variation in evaluation of poplar lignin content. By measuring the structural variation, the lignin content of the poplar can be accurately judged, superior plants with high-quality wood quality characters can be accurately and efficiently screened in the early growth stage of the poplar, the breeding period is effectively shortened, and a theoretical support is provided for molecular design breeding of the poplar wood quality.
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