Microbial agent for comprehensive utilization of saline-alkali soil and method for comprehensive utilization of saline-alkali soil

Abstract

The present disclosure relates to a microbial agent for comprehensive utilization of saline-alkali soil and a method for comprehensive utilization of saline-alkali soil, the microbial agent comprising a main agent and an auxiliary agent; the main agent comprising a tree-like bacillus, and the auxiliary agent comprising a tobacco arthrobacter; the tree-like bacillus is named as tree-like bacillus Paenibacillus dendritiformis , with a preservation number of CGMCC No.14473; and the tobacco arthrobacter is named as Arthrobacter nicotinovorans , with a preservation number of CGMCC No.33646. The microbial agent containing the tree-like bacillus for comprehensive utilization of saline-alkali soil can achieve a good effect of saline-alkali soil improvement.

Description

Technical Field

[0001] This application belongs to the field of agricultural biotechnology, specifically relating to a microbial agent and a method for the comprehensive utilization of saline-alkali land. Background Technology

[0002] Saline soils cover at least 10% of the world's arable land, with approximately 1 billion hectares at risk. Sustainable management of these soils is crucial for meeting food needs. Saline-alkali land is a prevalent soil type globally, particularly common in arid and semi-arid regions. Due to excessive salt content and deteriorated soil structure, these soils suffer from poor quality, hindered root growth, and reduced water and nutrient absorption efficiency, leading to the degradation of agricultural ecosystems and severely restricting the sustainability of agricultural production. Saline-alkali soils are typically rich in salt and alkaline substances; long-term soil salinization easily leads to soil hardening, nutrient imbalance, and reduced soil microbial diversity, resulting in low crop yields in saline-alkali areas. Summary of the Invention

[0003] The purpose of this disclosure is to provide a microbial agent and a method for the comprehensive utilization of saline-alkali land, which can achieve good results in improving saline-alkali land.

[0004] To achieve the above objectives, the first aspect of this disclosure provides a microbial agent for the comprehensive utilization of saline-alkali land, the microbial agent comprising a main agent and a secondary agent; the main agent comprises Bacillus densatilis, and the secondary agent comprises Arthrobacter nicotineus;

[0005] The classification of the dendritic spore-forming bacteria is dendritic spore-forming bacteria. Paenibacillus dendritiformis The accession number is CGMCC No. 14473; the classification name of the *Arthrobacterium tumefaciens* is... Arthrobacter nicotinovorans The accession number is CGMCC No. 33646.

[0006] Optionally, in the microbial agent, the ratio of the number of viable bacteria in the main agent to the number of viable bacteria in the auxiliary agent is 1:(0.2-1.3).

[0007] Optionally, the microbial agent is a solid preparation, wherein the viable count of the main microbial agent is 10. 5 Up to 10 12 CFU / g; or,

[0008] The microbial agent is a liquid, and the viable count of the main microbial agent in the microbial agent is 10. 5 Up to 10 12 CFU / mL.

[0009] Optionally, the co-bacterial agent further includes Bacillus licheniformis and / or Bacillus amyloliquefaciens;

[0010] The classification of Bacillus licheniformis is Bacillus licheniformis. Bacillus licheniformis The accession number is CGMCC No. 11235; the Bacillus amyloliquefaciens was purchased from the China Agricultural Microbial Culture Collection Center, accession number ACCC 19745.

[0011] Optionally, the microbial agent contains the Bacillus dendriticus, Bacillus licheniformis, Bacillus nicotineus, and Bacillus amyloliquefaciens.

[0012] Optionally, in the microbial agent, the ratio of the number of viable Bacillus licheniformis, the number of viable Arthrobacter nicotineus, and the number of viable Bacillus amyloliquefaciens is 1:(0.75-2):(0.75-2.5).

[0013] Optionally, in the microbial agent, the ratio of the number of viable Bacillus licheniformis, the number of viable Arthrobacter nicotineus, and the number of viable Bacillus amyloliquefaciens is 1:(0.75-1.3):(0.75-1.3).

[0014] Optionally, the microbial agent contains a culture medium;

[0015] The culture medium includes one or more of LB medium, tryptic soy liquid medium, broth medium and beef extract agar medium.

[0016] The second aspect of this disclosure provides a method for the comprehensive utilization of saline-alkali land, the method comprising: applying the microbial agent described in the first aspect of this disclosure to the saline-alkali land.

[0017] Optionally, the application rate of the microbial agent is 10 per square meter of the saline-alkali land. 4 Up to 10 11 CFU.

[0018] Through the above technical solution, this disclosure utilizes a compound microbial agent containing Bacillus dendriticus and Bacillus nicotineus for the comprehensive utilization of saline-alkali land. Through the synergistic effect of the compound Bacillus dendriticus and Bacillus nicotineus, it effectively improves soil structure, increases nutrient content, thereby reducing the concentration of salt ions in the soil, and effectively increases the number of culturable microorganisms in the soil, creating a more suitable environment for crop growth, significantly improving the arability of saline-alkali land, and increasing crop yield and quality.

[0019] Other features and advantages of this disclosure will be described in detail in the following detailed description section.

[0020] Information on the preservation of biological materials

[0021] The disclosed dendritic spore-forming bacteria Paenibacillus dendritiformis It is deposited at the China General Microbiological Culture Collection Center, located at No. 3, Courtyard 1, Beichen West Road, Chaoyang District, Beijing, Institute of Microbiology, Chinese Academy of Sciences, on July 31, 2017, with accession number CGMCC No. 14473.

[0022] The disclosed Bacillus licheniformis Bacillus licheniformis It is deposited at the China General Microbiological Culture Collection Center, located at No. 3, Courtyard 1, Beichen West Road, Chaoyang District, Beijing, Institute of Microbiology, Chinese Academy of Sciences, on August 13, 2015, with accession number CGMCC No. 11235.

[0023] The disclosed *Artemisia annua* ( Arthrobacter nicotinovorans The strain is deposited at the China General Microbiological Culture Collection Center, located at No. 3, Courtyard 1, Beichen West Road, Chaoyang District, Beijing, Institute of Microbiology, Chinese Academy of Sciences. The deposit date is February 24, 2025, and the accession number is CGMCC No. 33646. Detailed Implementation

[0024] The following provides a detailed description of specific embodiments of this disclosure. It should be understood that the specific embodiments described herein are for illustrative and explanatory purposes only and are not intended to limit this disclosure.

[0025] The first aspect of this disclosure provides a microbial agent for the comprehensive utilization of saline-alkali land, the microbial agent comprising a main agent and a secondary agent; the main agent comprises Bacillus densatilis, and the secondary agent comprises Arthrobacter nicotineus;

[0026] The classification of the dendritic spore-forming bacteria is dendritic spore-forming bacteria. Paenibacillus dendritiformis The accession number is CGMCC No. 14473; the classification name of the *Arthrobacterium tumefaciens* is... Arthrobacter nicotinovorans The accession number is CGMCC No. 33646.

[0027] The inventors of this disclosure combine *Bacillus densatilis* and *Arthrobacter nicotineus* to obtain a microbial agent for the improvement of saline-alkali land. Through the synergistic effect between specific strains, the texture and fertility of alkaline soil are improved, increasing crop yield and quality. This is an effective way to improve the quality of saline-alkali soil and enhance crop yield and quality. The microbial agent disclosed herein can effectively improve the properties of saline-alkali land, increase nutrient content (organic matter and available nitrogen, phosphorus, potassium, etc.), reduce soil salt concentration (sodium ions, magnesium ions, carbonate ions, etc.), and effectively increase the number of culturable microorganisms in the soil. The improved saline-alkali land is more suitable for crop growth, significantly improving its arable land and enhancing crop yield and quality.

[0028] According to one embodiment of this disclosure, the dosage form of the microbial agent includes liquid and / or solid. When the microbial agent is a solid, it can be applied directly or prepared as a liquid for application. The liquid can be applied directly after being cultured in a liquid culture medium or prepared as a liquid agent after being cultured for application.

[0029] According to one embodiment of this disclosure, the microbial agent contains a culture medium; the culture medium includes one or more of LB medium, tryptic soy broth (TSB) medium, broth medium, and beef extract agar medium.

[0030] According to one embodiment of this disclosure, a method for culturing *Bacillus dendriticis*, *Bacillus licheniformis*, *Arthrobacter nivea*, and *Bacillus amyloliquefaciens* may include: inoculating the bacterial strains into a culture medium for cultivation; the culture medium may be liquid or solid, and there are no particular limitations on the specific type. The cultivation conditions for the bacterial strains are not particularly limited and can be common conditions. For example, *Bacillus dendriticis* may be cultured on a shaker at a temperature of 28-37°C for 1-3 days. *Bacillus licheniformis* may be cultured on a shaker at a temperature of 28-37°C for 1-3 days. *Arthrobacter nivea* may be cultured on a shaker at a temperature of 28-37°C for 1-3 days. *Bacillus amyloliquefaciens* may be cultured on a shaker at a temperature of 28-35°C for 1-3 days. During the cultivation process, the concentration of viable bacteria can be obtained using conventional methods, such as platelet counting or OD value observation.

[0031] According to one embodiment of this disclosure, the cultured microbial agent can be further processed into a more conveniently stored dosage form for use through steps including aseptic filtration and freeze drying.

[0032] According to one embodiment of this disclosure, the microbial agent is a solid preparation, and in the microbial agent, the viable count of the main microbial agent is 10. 5 Up to 10 12CFU / g, preferably 10 8 Up to 10 10 CFU / g, including but not limited to 10 5 CFU / g, 0.2×10 6 CFU / g, 0.5×10 6 CFU / g, 0.8×10 6 CFU / g, 10 6 CFU / g, 0.2×10 7 CFU / g, 0.5×10 7 CFU / g, 0.8×10 7 CFU / g, 10 7 CFU / g, 0.2×10 8 CFU / g, 0.5×10 8 CFU / g, 0.8×10 8 CFU / g, 10 8 CFU / g, 0.2×10 9 CFU / g, 0.5×10 9 CFU / g, 0.8×10 9 CFU / g, 10 9 CFU / g, 0.2×10 10 CFU / g, 0.5×10 10 CFU / g, 0.8×10 10 CFU / g, 10 10 CFU / g, 0.2×10 11 CFU / g, 0.5×10 11 CFU / g, 0.8×10 11 CFU / g, 10 11 CFU / g, 0.2×10 12 CFU / g, 0.5×10 12 CFU / g, 0.8×10 12 CFU / g, 10 12 CFU / g, or a range of any two thereof; or, the microbial agent is a liquid preparation, and the viable count of the main agent is 10. 5 Up to 10 12 CFU / mL, preferably 10 7 Up to 10 9 CFU / mL, including but not limited to 10 5 CFU / mL, 0.2×10 6 CFU / mL, 0.5×10 6 CFU / mL, 0.8×10 6 CFU / mL, 10 6 CFU / mL, 0.2×107 CFU / mL, 0.5×10 7 CFU / mL, 0.8×10 7 CFU / mL, 10 7 CFU / mL, 0.2×10 8 CFU / mL, 0.5×10 8 CFU / mL, 0.8×10 8 CFU / mL, 10 8 CFU / mL, 0.2×10 9 CFU / mL, 0.5×10 9 CFU / mL, 0.8×10 9 CFU / mL, 10 9 CFU / mL, 0.2×10 10 CFU / mL, 0.5×10 10 CFU / mL, 0.8×10 10 CFU / mL, 10 10 CFU / mL, 0.2×10 11 CFU / mL, 0.5×10 11 CFU / mL, 0.8×10 11 CFU / mL, 10 11 CFU / mL, 0.2×10 12 CFU / mL, 0.5×10 12 CFU / mL, 0.8×10 12 CFU / mL, 10 12 CFU / mL, or a range of any two of these values; such ranges are beneficial for improving the physical and chemical properties of the soil, increasing nutrient content, reducing salt concentration and pH value, and increasing the number of culturable microorganisms.

[0033] According to one embodiment of this disclosure, in the microbial agent, the ratio of the number of viable bacteria in the main agent to the number of viable bacteria in the auxiliary agent is 1:(0.2-1.3), including but not limited to 1:0.2, 1:0.25, 1:0.5, 1:0.75, 1:1, 1:1.25, 1:1.3, or any combination thereof; the above ratio is beneficial to promote the synergistic effect of the main agent and the auxiliary agent, and improve the improvement effect of saline-alkali land.

[0034] According to one embodiment of this disclosure, the co-bacterial agent further includes Bacillus licheniformis and / or Bacillus amyloliquefaciens; the Bacillus licheniformis is classified as Bacillus licheniformis. Bacillus licheniformis The accession number is CGMCC No. 11235; the classification and nomenclature of the Bacillus amyloliquefaciens was purchased from the China Agricultural Microbial Culture Collection Center, with accession number ACCC 19745.

[0035] According to one embodiment of this disclosure, the co-bacterial agent includes *Arthrobacter nicotineus* and *Bacillus licheniformis*, that is, the microbial agent contains *Bacillus dendriticus*, *Arthrobacter nicotineus*, and *Bacillus licheniformis*; in the microbial agent, the ratio of the number of viable *Bacillus licheniformis* to the number of viable *Arthrobacter nicotineus* is 1:(0.75-2).

[0036] According to one embodiment of this disclosure, the co-bacterial agent includes *Arthrobacter nicotineus* and *Bacillus amyloliquefaciens*, that is, the microbial agent contains *Bacillus dendriticus*, *Arthrobacter nicotineus*, and *Bacillus amyloliquefaciens*; in the microbial agent, the ratio of the number of viable *Arthrobacter nicotineus* to the number of viable *Bacillus amyloliquefaciens* is 1:(0.75-1.6).

[0037] To improve the soil improvement effect, according to one embodiment of this disclosure, the auxiliary microbial agent includes *Bacillus nicotineus*, *Bacillus licheniformis*, and *Bacillus amyloliquefaciens*, that is, the microbial agent contains *Bacillus dendriticus*, *Bacillus licheniformis*, *Bacillus nicotineus*, and *Bacillus amyloliquefaciens*.

[0038] According to one embodiment of this disclosure, in the microbial agent, the ratio of the viable number of Bacillus licheniformis, the viable number of Bacillus nicotineus, and the viable number of Bacillus amyloliquefaciens is 1:(0.75-2):(0.75-2.5), preferably 1:(0.75-1.3):(0.75-1.3). The above ratio is beneficial to further improve the soil improvement effect, improve the arability of saline-alkali land, and improve the yield and quality of crops.

[0039] According to one embodiment of this disclosure, the microbial agent is a liquid microbial agent, and the method for preparing the liquid microbial agent may include: mixing and compounding liquid microbial agent A and liquid microbial agent B, wherein liquid microbial agent A contains Bacillus dendriticus CGMCC No. 14473, and liquid microbial agent B contains Arthrobacter nivea CGMCC No. 33646, optionally Bacillus licheniformis CGMCC No. 11235 and optionally Bacillus amyloliquefaciens ACCC 19745, and liquid microbial agents A and B respectively contain liquid culture medium and / or buffer solution.

[0040] According to one embodiment of this disclosure, the microbial agent is a solid agent, and the preparation method may include mixing the cultured strains or loading them onto a solid carrier. There are no particular limitations on the type of solid carrier.

[0041] The second aspect of this disclosure provides a method for the comprehensive utilization of saline-alkali land, the method comprising: applying the microbial agent described in the first aspect of this disclosure to the saline-alkali land.

[0042] According to one embodiment of this disclosure, the application rate of the microbial agent is 10 per square meter of the saline-alkali land. 4 Up to 10 11 CFU, preferably 10 7 Up to 10 11 CFU, including but not limited to 10 4 CFU, 0.2×10 5 CFU, 0.5×10 5 CFU, 0.8×10 5 CFU, 10 5 CFU, 0.2×10 6 CFU, 0.5×10 6 CFU, 0.8×10 6 CFU, 10 6 CFU, 0.2×10 7 CFU, 0.5×10 7 CFU, 0.8×10 7 CFU, 10 7 CFU, 0.2×10 8 CFU, 0.5×10 8 CFU, 0.8×10 8 CFU, 10 8 CFU, 0.2×10 9 CFU, 0.5×10 9 CFU, 0.8×10 9 CFU, 10 9 CFU, 0.2×10 10 CFU, 0.5×10 10 CFU, 0.8×10 10 CFU, 10 10 CFU, 0.2×10 11 CFU, 0.5×10 11 CFU, 0.8×10 11 CFU, 10 11 CFU, or a range of values ​​consisting of any two of them; the above application rates are beneficial for improving soil structure, increasing soil fertility, and improving crop yield and quality.

[0043] According to one embodiment of this disclosure, there are no special requirements for the application method of microbial inoculants; they can be applied together with base fertilizer or top dressing.

[0044] The present invention will be further described in detail below with reference to the embodiments, but the scope of the present invention is not limited to the following embodiments.

[0045] Unless otherwise specified, all culture media used are commercially available products.

[0046] The Bacillus amyloliquefaciens ACCC 19745 used in the examples was purchased from the China Agricultural Microbial Culture Collection Center.

[0047] Test method for ion content: Industry standard "Methods for Analysis of Agricultural Chemicals in Soil" (NY / T 1121-2006), in which cation Ca... 2+ and Mg 2+ Using EDTA complexometric titration, Na + and K + Flame photometry was used; anionic SO4 2- Using EDTA indirect titration, Cl - AgNO3 titration method, HCO3 - Using phenolphthalein indicator solution method, NO3 - Spectrophotometry was used.

[0048] Test method for electrical conductivity (EC): Turn on the power of the pH meter and electrical conductivity meter and preheat for 30 minutes, then calibrate with a standard solution. Weigh the soil, add deionized water at a water-to-soil ratio of 5:1, shake on a shaker for 30 minutes, let stand for 30 minutes, measure the conductivity of the sample supernatant, and record the instrument reading after it stabilizes.

[0049] Test methods for available nitrogen, phosphorus and potassium content: Available nitrogen content is determined by the Kjeldahl method; available phosphorus content in neutral and alkaline soils is determined by the Olsen method (sodium bicarbonate extraction method), and available phosphorus content in acidic soils is determined by the Bray-1 method (hydrochloric acid-ammonium fluoride extraction method); available potassium content is determined by nitric acid extraction-flame photometry.

[0050] Test method for organic matter content: potassium dichromate oxidation method, [National Standard] GB 9834-1988.

[0051] Method for testing microbial abundance: Accurately weigh 2.0 g of soil sample into a 50 mL sterile Erlenmeyer flask, add 18 mL of phosphate buffer solution (PBS, pH=7.2-7.4, Sigma-Aldrich), vortex for 30 s, then place on a shaker and shake at 200 r / min for 30 min (25℃). Serially dilute the obtained mixture, and inoculate 0.1 mL into sterile petri dishes for culture and calculation. Specifically, the bacterial count (CFU / g) = average number of colonies (cFU / g) × dilution factor × volume of mother culture suspension (mL) / mass (g) × 1 / inoculum size (mL). Where the dilution factor (10... -1 Up to 10 -7The mother liquor bacterial suspension volume was 20 mL, the mass was 2 g, the inoculum amount was 0.1 mL, and the average number of colonies was 30-300. For bacteria: LB medium (culture medium formula: 10 g tryptone, 5 g yeast extract, 10 g sodium chloride, add double-distilled water to 1000 mL, adjust pH to 7.2 with 5 mol / L sodium hydroxide (approximately 0.2 mL), sterilize at 121℃ for 30 min) was used for culturing at 37℃ for 2 days; for fungi: PDA medium (culture medium formula: 200 g peeled potato, 20 g glucose, add distilled water to 1000 mL, add 0.3 mL of 1% streptomycin solution per 100 mL of medium) was used for culturing at 37℃ for 3 days; for actinomycetes: modified Gao's No. 1 medium (culture medium formula: 20 g soluble starch, 1 g potassium nitrate, 0.5 g sodium chloride, 0.5 g potassium dihydrogen phosphate, 0.5 g magnesium sulfate, 0.01 g ferrous sulfate, add distilled water to 1000 mL, adjust pH to 7.4-7.6, add 1 mL of 3% potassium dichromate per 300 mL of medium) was used for culturing at 28℃ for 7 days.

[0052] In the examples and comparative examples, the preparation method of the microbial inoculant is as follows: liquid inoculant A containing the main inoculant is mixed and compounded with liquid inoculant B containing the auxiliary inoculant;

[0053] Preparation method of liquid bacterial agent A: Inoculate Bacillus dendriticus CGMCC No.14473 into 200 mL of LB liquid medium and incubate at 35℃ with shaking for 24-36 h;

[0054] Preparation method of liquid bacterial agent B: Inoculate the auxiliary bacterial agent into 200 mL of LB liquid medium and incubate at 35℃ with shaking for 24-36 h.

[0055] LB medium formula: 10 g tryptone, 5 g yeast extract, 10 g sodium chloride, add double-distilled water to 1000 mL, adjust pH to 7.2 with 5 mol / L NaOH (about 0.2 mL), sterilize at 121℃ for 30 min.

[0056] The auxiliary bacterial agents were selected from Bacillus licheniformis CGMCC No. 11235, Bacillus nicotineus CGMCC No. 33646, and Bacillus amyloliquefaciens ACCC 19745. The specific species were selected according to the bacterial strains required for the experimental treatment.

[0057] Test case

[0058] Soil samples were collected from farmland in the Ningxia Yellow River irrigation area, mainly planted with corn. The soil type was gray-calcareous soil. Soil culture experiments were conducted, and the microbial agent was applied to the soil for 120 days. Soil parameters are listed in Table 2, and the evaluation results of the microbial agent are listed in Tables 3-5 (except for the microbial agent, the other operations were the same as those for corn planting in the local area).

[0059] Experimental treatment 1: Blank control group, no bacterial agent was applied.

[0060] Experimental Treatment 2: A compound microbial agent containing Bacillus denticulatus CGMCC No.14473 (Y1), Bacillus licheniformis CGMCC No.11235 (X1 in Table 1), Bacillus nicotineus CGMCC No.33646 (X2 in Table 1) and Bacillus amyloliquefaciens ACCC19745 (X3 in Table 1) was applied to the soil for soil treatment. The composition of the agent and the application rate relative to each square meter of soil are shown in Table 1.

[0061] Experimental treatments 3-7: Soil treatments were carried out using the method of experimental treatment 2, with the difference being that the composition and application rate of the microbial agent were adjusted. See Table 1 for details.

[0062] Experimental Treatment 8: Soil was treated using the method of Experimental Treatment 2, except that no auxiliary microbial agent was added. See Table 1 for details.

[0063] Experimental Treatment 9: Soil was treated using the method of Experimental Treatment 2, except that Bacillus dendriticis CGMCC No. 14473 was replaced with another Bacillus dendriticis agent screened together with it (Y11 in Table 1), see Table 1 for details.

[0064] Experimental Treatment 10: Soil was treated using the method of Experimental Treatment 2, except that Bacillus nicotineus CGMCC No. 33646 was replaced with another Bacillus nicotineus that was screened together with it (X22 in Table 1), see Table 1 for details.

[0065] Experimental Treatment 11: Commercially available saline-alkali land improvement microbial agent A (Shandong Yihao Biotechnology, liquid spore compound microbial agent product, live bacteria content 1 billion / ml, effective ingredients >90wt%), see Table 1 for details.

[0066] Experimental treatment 12: Commercially available saline-alkali land improvement bacterial agent B (Zhengzhou Baiyibao Biotechnology Co., Ltd., EM strain, live bacteria content 2 billion / ml), see Table 1 for details.

[0067] Table 1

[0068]

[0069] Table 2

[0070]

[0071] Table 3

[0072]

[0073] Table 4

[0074]

[0075] Table 5

[0076]

[0077] Based on the above data, it can be seen that the microbial agent disclosed herein has a good improvement effect on saline-alkali land, effectively increasing soil nutrient content, reducing soil salt ion concentration, and effectively increasing the number of culturable microorganisms in the soil, which is beneficial to improving crop yield and quality. Furthermore, a comparison of treatments 2-3 and 4-7 shows that the presence of *Bacillus densatilis*, *Bacillus licheniformis*, *Arthrobacter nivea*, and *Bacillus amyloliquefaciens* in the agent can improve the treatment effect. A comparison of treatments 2 and 3 shows that when the ratio of viable *Bacillus licheniformis*, *Arthrobacter nivea*, and *Bacillus amyloliquefaciens* is within the range of 1:(0.75-2):(0.75-2.5), the treatment effect on saline-alkali land is better. A comparison of treatments 4 and 5 shows that when the ratio of the main agent to the auxiliary agent is within the range of 1:(0.2-1.3), it can further increase soil nutrient content, reduce soil salt ion concentration, and effectively increase the number of culturable microorganisms in the soil.

[0078] The preferred embodiments of this disclosure have been described in detail above. However, this disclosure is not limited to the specific details of the above embodiments. Within the scope of the technical concept of this disclosure, various simple modifications can be made to the technical solutions of this disclosure, and these simple modifications all fall within the protection scope of this disclosure.

[0079] It should also be noted that the various specific technical features described in the above embodiments can be combined in any suitable manner without contradiction. To avoid unnecessary repetition, this disclosure will not describe the various possible combinations separately.

[0080] Furthermore, various different embodiments of this disclosure can be combined in any way, as long as they do not violate the spirit of this disclosure, they should also be regarded as the content disclosed in this disclosure.

Claims

1. A microbial inoculant for comprehensive utilization of saline-alkali soil, characterized in that, The microbial agent comprises a main agent and an auxiliary agent; the main agent comprises Paenibacillus dendritiformis, and the auxiliary agent comprises Arthrobacter nicotinovorans. The taxonomic name of the Paenibacillus suincola is Paenibacillus suincola Paenibacillus dendritiformis , and the accession number is CGMCC No. 14473; the taxonomic name of the Arthrobacter nicotianae is Arthrobacter nicotianae Arthrobacter nicotinovorans , and the accession number is CGMCC No. 33646.

2. The microbial inoculant of claim 1, wherein, In the microbial agent, the ratio of the viable cell count of the main agent to the viable cell count of the auxiliary agent is 1: (0.2-1.3).

3. The microbial inoculant of claim 2, wherein, The microbial agent is a solid agent, in which the viable cell count of the main microbial agent is 10 5 to 10 12 CFU / g; or, The microbial agent is a liquid agent, in which the viable cell count of the main microbial agent is 10 5 to 10 12 CFU / mL.

4. The microbial inoculant of claim 2, wherein, The auxiliary agent further comprises Bacillus licheniformis and / or Bacillus amyloliquefaciens. The taxonomic name of the said Bacillus licheniformis is Bacillus licheniformis Bacillus licheniformis , and the preservation number is CGMCC No.11235; the said Bacillus amyloliquefaciens is purchased from China Agricultural Microbial Culture Collection Center, and the number is ACCC19745.

5. The microbial inoculant of claim 4, wherein, The microbial agent comprises the Paenibacillus dendritiformis, the Bacillus licheniformis, the Arthrobacter nicotinovorans and the Bacillus amyloliquefaciens.

6. The microbial inoculant of claim 5, wherein, In the microbial agent, the ratio of the viable cell count of the Bacillus licheniformis to the viable cell count of the Arthrobacter nicotinovorans to the viable cell count of the Bacillus amyloliquefaciens is 1: (0.75-2): (0.75-2.5).

7. The microbial inoculant of claim 5, wherein, In the microbial agent, the ratio of the viable cell count of the Bacillus licheniformis to the viable cell count of the Arthrobacter nicotinovorans to the viable cell count of the Bacillus amyloliquefaciens is 1: (0.75-1.3): (0.75-1.3).

8. The microbial inoculant of claim 1, wherein, The microbial agent comprises a culture medium; The culture medium comprises one or more of LB medium, tryptone soy broth liquid medium, broth medium and beef extract agar medium.

9. A method for comprehensive utilization of saline-alkali soil, characterized in that, The method comprises: applying the microbial agent of any one of claims 1-8 to saline-alkali soil.

10. The method of claim 9, wherein, The application amount of the microbial inoculant is 10 4 to 10 11 CFU per square meter of the saline-alkali soil.

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