Synthesis and application of hypochlorous acid fluorescence probe based on phenothiazine cinnamyl aldehyde
The fluorescent probe PTZ-CA prepared from phenothiazine cinnamaldehyde solves the problems of long synthesis steps and poor photostability of existing fluorescent probes, achieves high sensitivity and rapid response hypochlorous acid detection, and is suitable for biomedical imaging.
CN120682254APending Publication Date: 2025-09-23XIANGTAN UNIV
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Patent Information
- Application Number
- CN202510940263.9
- Authority / Receiving Office
- CN · China
- Patent Type
- Applications(China)
- Current Assignee / Owner
- Filing Date
- 2025-07-08
- Publication Date
- 2025-09-23
AI Technical Summary
Technical Problem
Existing fluorescent probes for detecting hypochlorous acid have long synthesis steps and poor photostability, which cannot meet the needs of biomedical imaging.
Method used
Phenothiazine cinnamaldehyde was used as the core structure of the fluorescent probe. Through simple synthetic steps, it reacted with 4-(dimethylamino)cinnamaldehyde and piperidine under nitrogen protection to prepare a reddish-brown fluorescent probe PTZ-CA for the detection of hypochlorous acid.
Benefits of technology
It achieves high sensitivity, fast response and good selectivity for hypochlorous acid detection, is suitable for imaging in living cells, has high photostability and a detection range from 1μM to 35μM, with a detection limit of 0.32μM.
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Abstract
The invention relates to synthesis and application of a hypochlorous acid fluorescent probe based on phenothiazine cinnamyl aldehyde. The structural formula of the probe is shown in the specification. The invention provides a preparation method for synthesizing the fluorescent probe by taking phenothiazine (PTZ) and 4-(dimethylamino) cinnamyl aldehyde as raw materials. The fluorescent probe has the characteristics of high sensitivity, quick response, low cytotoxicity and the like, and is suitable for detecting hypochlorous acid. Firstly, the fluorescent probe has weak fluorescence at the position of 560 nm, after the fluorescent probe responds to hypochlorous acid, the fluorescence at the position of 560 nm is remarkably enhanced, and good linear correlation is shown in the range of 1-35 [mu] M; in addition, the fluorescent probe has good selectivity on hypochlorous acid, and is not influenced by various kinds of cations, anions, biological mercaptan and active oxygen; furthermore, the interaction between the fluorescent probe and hypochlorous acid is rapid, and the response time is within 30s; finally, in cells, the fluorescent probe shows low toxicity and can be used for detecting hypochlorous acid in living cells.
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