741 poplar PtoWRKY24 gene promoter responding to multiple stress and application of 741 poplar PtoWRKY24 gene promoter

By introducing the PtoWRKY24 gene promoter into poplar, the problem of the lack of poplar stress-resistant gene promoters that respond to multiple stresses in the existing technology was solved, the stress resistance of poplar under multiple stresses was enhanced, and a theoretical basis for molecular breeding was provided.

CN120843518APending Publication Date: 2025-10-28HEBEI AGRICULTURAL UNIV.
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Patent Information

Application Number
CN202510998860.7
Authority / Receiving Office
CN · China
Patent Type
Applications(China)
Current Assignee / Owner
Filing Date
2025-07-21
Publication Date
2025-10-28

AI Technical Summary

Technical Problem

The existing technology lacks poplar stress resistance gene promoters that can specifically respond to multiple biotic/abiotic stresses, which limits the progress of molecular breeding for improving poplar stress resistance.

Method used

The PtoWRKY24 gene promoter and its recombinant vector are provided. They are introduced into plant cells by Agrobacterium transformation, and their stress response activity is analyzed in combination with the reporter gene GUS to verify their specific response function under multiple stresses.

Benefits of technology

The study verified that the PtoWRKY24 promoter has specific responsiveness to salt stress, drought, hormone stress, and pest stress, providing a theoretical basis for molecular breeding to improve the stress resistance of poplar trees and enhancing the stress response of plants.

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Abstract

The invention provides a 741 poplar PtoWRKY24 gene promoter responding to multiple stress and application of the 741 poplar PtoWRKY24 gene promoter, and belongs to the technical field of molecular breeding. According to the invention, a PtoWRKY24 promoter is heterologously expressed in a model plant tobacco, and a reporter gene GUS is combined, so that the activity change of the promoter under stress conditions of high salt, drought, cotton bollworm, hormone and the like is analyzed, and whether the promoter has a specific stress response function is verified. The invention provides a new clue for knowing the stress resistance function and molecular mechanism of the PtoWRKY24 gene, lays a theoretical foundation for improving the stress resistance and adaptability of the poplar through molecular breeding, and has important significance for promoting plant stress resistance molecular breeding and ecological restoration.
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