Enhancer regulatory elements for transcriptional regulation of human ltf gene expression and uses

By identifying and constructing transcriptional regulatory elements and vectors specific to the human LTF gene, the problem of low LTF expression efficiency in bovine mammary glands has been solved, achieving high-efficiency expression and supporting the development of mammary gland bioreactors and functional dairy products.

CN120866319BActive Publication Date: 2026-05-22NORTHWEST A & F UNIV
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Patent Information

Application Number
CN202511006832.9
Authority / Receiving Office
CN · China
Patent Type
Patents(China)
Current Assignee / Owner
Filing Date
2025-07-22
Publication Date
2026-05-22
Estimated Expiration
2045-07-22

AI Technical Summary

Technical Problem

Existing technologies cannot explain the efficient expression of human lactoferrin (LTF) in bovine mammary glands. There is a lack of systematic discovery and validation of human-specific regulatory elements, and existing expression vectors rely on species-conserved elements, making it difficult to achieve high LTF expression at the human level.

Method used

Using enhancer regulatory elements and vectors that regulate the transcriptional expression of the human LTF gene, key regulatory sequences for extremely high expression of LTF in human milk were identified through multi-omics analysis such as ATAC-seq and ChIP-seq. The pGL4.17 and pcDNA3.1 vectors were constructed to achieve efficient cross-species expression.

Benefits of technology

It significantly activates reporter gene expression in bovine mammary epithelial cells, increasing expression efficiency to over 90% of the natural human level, reducing R&D cycle and cost, providing an efficient expression tool, and supporting the development of mammary bioreactors and functional dairy products.

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Abstract

The application discloses an enhancer regulatory element for transcriptional regulation and expression of a human LTF gene and application thereof, wherein the sequence of the regulatory element is SEQ ID No. 1. Experiments prove that the human-specific regulatory element can significantly activate the expression of a reporter gene (up to 14.93 times) in a bovine mammary epithelial cell line (MACT), breaks through the limitation of a species-conserved element in the prior art, and an expression system designed based on the element can realize efficient expression of LTF at a human level, thereby providing a new tool for mammary gland bioreactors and development of functional dairy products.
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