Kit and method of infection for efficient infection of aav 3d organoids
By using the synergistic effect of a detachable culture scaffold and a specific culture medium, the low efficiency and complex operation of existing AAV-infected 3D organoids methods have been solved, achieving efficient virus delivery and organoid survival. It is applicable to a variety of organoids and automated platforms and is compatible with common instruments on the market.
Patent Information
- Application Number
- CN202511251059.2
- Authority / Receiving Office
- CN · China
- Patent Type
- Patents(China)
- Current Assignee / Owner
- Filing Date
- 2025-06-11
- Publication Date
- 2026-07-24
- Estimated Expiration
- 2045-06-11
AI Technical Summary
Existing methods for AAV-infected 3D organoids suffer from problems such as complex operation, long time consumption, low infection efficiency, low infection success rate, and easy organoid death, making it difficult to achieve efficient virus delivery while maintaining the three-dimensional structure and physiological polarity of the organoids.
A kit and corresponding infection method, including a removable culture scaffold device and a specific AAV infection-specific culture medium, were used to maintain the three-dimensional morphology and activity of organoids by utilizing the synergistic effect of the TLTD infection-promoting system and low-concentration DHA-S components, combined with viral infection under dynamic conditions.
It significantly improves AAV infection efficiency, has high organoid survival rate, is suitable for efficient transduction of trace viral resources, is suitable for experimental scenarios with limited clinical sample size, is easy to operate and applicable to 3D organoids from various sources, is compatible with common 96-well plate instruments on the market, and is suitable for medium- to high-throughput experimental environments.