Traditional Chinese medicine composition for improving glomerular capillary microcirculation

Abstract

The invention belongs to the technical field of traditional Chinese medicines, and particularly relates to a traditional Chinese medicine composition for improving glomerular capillary microcirculation. The traditional Chinese medicine composition is prepared from the following raw materials in parts by weight: 10-20 parts of radix paeoniae alba, 4-14 parts of lucid ganoderma, 1-11 parts of wine-treated rhubarb, 4-14 parts of ground beeltle, 7-17 parts of astragalus membranaceus, 4-14 parts of stiff silkworm, 1-11 parts of leech and 1-11 parts of honey-fried licorice root. The traditional Chinese medicine composition is used for improving glomerular capillary microcirculation, and can effectively delay the progress of chronic nephritis, protect the renal function, reduce the occurrence of complications and improve the life quality of patients. Therefore, the method has a good application prospect.

Description

TECHNICAL FIELD

[0001] The present application belongs to the technical field of traditional Chinese medicine, and particularly relates to a traditional Chinese medicine composition for improving microcirculation of glomerular capillary. BACKGROUND

[0002] Chronic nephritis is a group of glomerular diseases characterized by edema, proteinuria, hypertension, and slow progression of renal function decline. It is a continuously progressive disease and can eventually develop into end-stage renal disease. Modern Chinese medicine doctors believe that the basic pathogenesis of chronic nephritis is a combination of deficiency and excess, with deficiency being the root and the lung, spleen and kidney being responsible. Kidney deficiency is the main problem, and evil is the standard. Wet, turbid and stasis are the main problems. The treatment emphasizes the importance of supporting the right, benefiting the wet, and removing the stasis. Qi, blood and water are treated together. In recent years, the reported treatment of chronic nephritis mainly includes the methods of tonifying the kidney and invigorating the spleen, benefiting qi and nourishing yin, promoting blood circulation to remove blood stasis, removing dampness and turbidity, and resolving phlegm and resolving phlegm.

[0003] Kidney microcirculation refers to the network of small blood vessels inside the kidney, including the capillary network of the glomerulus and the blood vessels around the renal tubules. These microvessels are essential for maintaining normal kidney function. After various causes lead to kidney disease, inflammation is enhanced and oxidative stress is intensified. These factors together cause thickening of the glomerular basement membrane, glomerular sclerosis, renal tubulointerstitial fibrosis, and intrarenal vascular lesions, affecting the normal operation of kidney microcirculation. Therefore, improving kidney microcirculation can effectively delay the progression of chronic nephritis, protect kidney function, reduce the occurrence of complications, and improve the quality of life of patients. In the prior art, there are related literature reports on improving kidney microcirculation by traditional Chinese medicine. For example, CN201210386997.X discloses a traditional Chinese medicine composition capable of improving kidney microcirculation.

[0004] From the perspective of improving the effect of drug treatment and the different reactions of patients to drugs, it is still necessary to develop more new drugs for improving the microcirculation of glomerular capillary. SUMMARY

[0005] In view of the problems in the prior art, the present application provides a traditional Chinese medicine composition for improving the microcirculation of glomerular capillary.

[0006] A traditional Chinese medicine composition is prepared from the following raw materials by weight:

[0007] Radix Paeoniae Alba 10-20 parts,

[0008] Lucidum 4-14 parts,

[0009] Rhubarb 1-11 parts,

[0010] Eupolyphaga 4-14 parts,

[0011] Radix Astragali 7-17 parts,

[0012] Eupolyphaga 4-14 parts,

[0013] Leech 1-11 parts,

[0014] Roasted Licorice 1-11 parts.

[0015] Preferably, it is made from the following raw materials by weight:

[0016] White Peony Root 14-16 parts,

[0017] Lucid Ganoderma 8-10 parts,

[0018] Rhubarb 5-7 parts,

[0019] Earthworm 8-10 parts,

[0020] Milk Vetch Root 11-13 parts,

[0021] Eupolyphaga 8-10 parts,

[0022] Leech 5-7 parts,

[0023] Roasted Licorice 5-7 parts.

[0024] Preferably, it is made from the following raw materials by weight:

[0025] White Peony Root 15 parts,

[0026] Lucid Ganoderma 9 parts,

[0027] Rhubarb 6 parts,

[0028] Earthworm 9 parts,

[0029] Milk Vetch Root 12 parts,

[0030] Eupolyphaga 9 parts,

[0031] Leech 6 parts,

[0032] Roasted Licorice 6 parts.

[0033] Preferably, the Rhubarb is Wine Rhubarb.

[0034] Preferably, it is a preparation made from the raw drug powder, water or organic solvent extract of the raw materials as active ingredients, with the addition of pharmaceutically acceptable adjuvants or auxiliary ingredients.

[0035] Preferably, the preparation is granules, pills, powders, decoction paste, tablets, capsules, solutions or dry powder sprays.

[0036] The present application also provides the use of the above-mentioned traditional Chinese medicine composition in the preparation of a medicament for improving microcirculation.

[0037] Preferably, the medicament is used for improving the microcirculation of glomerular capillary.

[0038] Chronic nephritis is a group of glomerular diseases characterized by edema, proteinuria, hypertension, and slow progression of renal dysfunction. It is a persistent progressive disease that can eventually develop into end-stage renal disease. Modern Chinese medical experts believe that the basic pathogenesis of chronic nephritis is a combination of deficiency and excess, with deficiency being the root cause. It is attributed to the lungs, spleen, and kidneys, with kidney deficiency being the main cause and evil excess being the main cause. Wet, turbid, and stasis are the main causes. Treatment emphasizes the importance of supporting the healthy, removing dampness, and removing blood stasis. Qi, blood, and water are treated together. In recent years, the reported treatment options for chronic nephritis mainly include kidney and spleen tonification, qi and yin nourishment, blood circulation, dampness removal, and phlegm and stasis removal. This prescription is composed of white peony root, ganoderma lucidum, wine rhubarb, earthworm, astragalus, silkworm chrysalis, leech, and licorice, among other herbs. It has the effects of promoting blood circulation, removing stagnation, tonifying deficiency, and removing blood stasis, and can significantly improve the microcirculation of glomerular capillary.

[0039] White peony root is bitter, sour, and slightly cold, and is meridian-entering to the liver and spleen. It has the effects of nourishing blood and yin, soothing the liver to stop pain, and calming the liver yang. It is used for irregular menstruation, abdominal pain during menstruation, metrorrhagia, spontaneous sweating, night sweats, liver qi disharmony in the lateral costal region, epigastric and abdominal pain, or pain in the limbs, liver yang hyperactivity headache or dizziness, etc. The Shennong Herbal states that it "has a bitter taste and is flat. It is used to treat abdominal pain caused by evil qi, remove blood stasis, break down accumulated masses, cold and heat, abdominal masses, stop pain, promote urination, and benefit qi." The Herbal Alternatives states that it "is used to regulate blood vessels, relax the middle, disperse evil blood, remove evil blood, remove water and qi, benefit the bladder and large and small intestines, resolve abscesses and swelling, and treat cold and heat, abdominal pain, and waist pain." The Thunder Cannon Processing Herbal states that it "is used to treat anger damaging the liver, accumulation in the chest and abdomen, and blood stasis between the waist and navel." In this prescription, white peony root regulates blood vessels, soothes the liver, relaxes the middle, promotes urination, and promotes the excretion of turbid yin such as urine, making it the monarch herb.

[0040] Ganoderma lucidum is sweet, flat, and non-toxic, and is meridian-entering to the heart, lungs, liver, and kidneys. It has the effects of benefiting qi and blood, calming the heart and mind, invigorating the stomach and kidneys, tonifying and strengthening, stopping cough and asthma, preventing cancer, and resisting cancer. It is used for deficiency, palpitation, insomnia, dizziness, fatigue, chronic cough and asthma, coronary heart disease, silicosis, and tumors, among others. The Shennong Herbal describes six types of ganoderma, "red ganoderma, bitter and flat. It is used to treat chest masses, benefit heart qi, tonify the center, increase wisdom and intelligence, and not forget. Long-term consumption can make the body light and not old, and prolong life and become a god." It is the monarch herb.

[0041] Rhubarb, when processed with wine, is bitter and cold in nature. It enters the spleen, stomach, large intestine, liver, and heart meridians. It has the effects of purging stagnation, clearing heat and purging fire, detoxifying, and promoting blood circulation and removing blood stasis. It is used for intestinal stagnation, constipation, hematemesis and epistaxis due to blood heat, red eyes, sore throat, and swollen gums caused by upward flaring of fire evil, sores and burns caused by heat toxins, amenorrhea and postpartum lochia retention due to blood stasis in women, masses and accumulations, traumatic injuries and other blood stasis syndromes, jaundice, gonorrhea, and other damp-heat syndromes. The *Shennong Bencao Jing* states that rhubarb "treats blood stasis, amenorrhea, chills and fever, breaks up masses and accumulations, removes retained fluids and undigested food, cleanses the intestines and stomach, promotes metabolism, facilitates the flow of water and grains, regulates the middle burner and aids digestion, and harmonizes the five internal organs." The *Bencao Jing Jizhu* states that it "treats blood stasis, amenorrhea, chills and fever, breaks up masses and accumulations, removes retained fluids and undigested food, cleanses the intestines and stomach, promotes metabolism, and facilitates the flow of water and grains." Rhubarb, with its bitter taste and downward-draining properties, clears blockages. When yin harmonizes with yang, chills and fever cease. Masses and accumulations are tangible pathogenic factors; rhubarb can clear them away, thus breaking them up. In this formula, rhubarb excels at breaking up blood stasis and promoting metabolism, possessing the effects of activating blood circulation, removing blood stasis, and eliminating accumulations. It opens the path for blood stasis to flow downwards, allowing the transformed blood stasis to be expelled, thus achieving the effect of "removing stagnant and stagnant substances," making it an assistant herb.

[0042] Leeches are salty, bitter, and neutral in nature; they are slightly toxic. They enter the liver meridian. They have the effect of breaking up blood stasis and removing blood stasis. They are used for amenorrhea due to blood stasis, abdominal masses, and injuries caused by traumatic injuries and other conditions involving blood stasis. Leeches are particularly effective at breaking up blood stasis and removing blood stasis, and their effect is quite strong, treating amenorrhea and abdominal masses. The *Shennong Bencao Jing* states that it is "salty and neutral in nature. It mainly removes stagnant blood, blood stasis, and amenorrhea. It breaks up blood masses, treats infertility, and promotes urination." The *Changsha Yaoxie* states that it "is good at breaking up accumulated blood and can dissolve hard masses." The *Yinpian Xincan* states that it "breaks up blood stasis, unblocks meridians, and treats blood stasis." Leeches also have the effect of promoting urination and breaking up accumulations; clinically, they are often used to treat edema, such as nephrotic syndrome and ascites due to cirrhosis, and are used as an adjuvant herb.

[0043] Ground beetle is salty and cold in nature; it is slightly toxic. It enters the liver meridian. It has the effects of breaking up blood stasis, removing blood stasis, and promoting tendon and bone healing. It is used for amenorrhea, postpartum blood stasis, abdominal masses, fractures, pain due to blood stasis, and lumbar sprains. The Shennong's Classic of Materia Medica states that it "treats cold and heat in the heart and abdomen, blood stasis and abdominal masses, breaks up hard masses and removes blood stasis." Lei Gong's Explanation of the Properties of Processed Medicinal Herbs states that "ground beetle is mainly used for blood disorders. The heart governs blood, the liver stores blood, and the spleen contains blood, hence it is used in all three." This formula combines ground beetle and leech to promote blood circulation and remove blood stasis, and with rhubarb processed with wine, it aligns with the view that "without removing blood stasis, new blood cannot be generated," adding blood-activating and stasis-removing herbs as assistant herbs.

[0044] Silkworm (Bombyx mori) is salty, pungent, and neutral in nature. It enters the liver and lung meridians. It has the effects of calming wind and stopping spasms, dispelling wind and relieving pain, and detoxifying and dispersing nodules. It is used for convulsions and epilepsy caused by internal liver wind and phlegm-heat accumulation; headaches, red eyes, sore throat, toothache caused by wind-heat and liver heat; scrofula, boils, erysipelas, and itchy rashes. *Yuqiu Yaoxie* states that it "activates blood circulation and unblocks meridians, dispels wind and relieves numbness." *Depei Bencao* states that it "calms toxic phlegm caused by rebellious fire, treats malaria caused by wind-heat attacking the liver, dispels wind-induced parasitic infestations, treats wind-heat phlegm accumulation causing lockjaw, treats infantile malnutrition, and stops metrorrhagia and leukorrhea in women." In this formula, silkworm (Bombyx mori) is the assistant herb for activating blood circulation and unblocking meridians.

[0045] Astragalus is sweet and slightly warm. It enters the spleen and lung meridians. It has the effects of tonifying qi and raising yang, strengthening wei qi and consolidating the exterior, promoting tissue regeneration and detoxification, and promoting diuresis and reducing edema. It is used for syndromes of spleen and lung qi deficiency or sinking of middle qi, spontaneous sweating due to deficiency of wei qi, carbuncles that do not ulcerate or ulcers that do not heal for a long time due to insufficient qi and blood, edema and scanty urine, numbness of limbs, joint pain or hemiplegia caused by qi deficiency and blood stasis, and thirst due to qi deficiency and fluid depletion. The *Mingyi Bielu* states that it "treats wind-evil qi in the uterus of women, expels stagnant blood between the five viscera, replenishes deficiencies in men, treats emaciation due to overwork, quenches thirst, relieves abdominal pain and diarrhea, benefits qi, and nourishes yin qi." The *Bencao Jingjie* states that "the deficiencies in the human body are varied and uneven, but they are all related to qi and blood. Astragalus is sweet and warm in nature; it warms qi to replenish deficiencies in form, and replenishes essence to benefit deficiencies in essence." In this formula, astragalus replenishes deficiency and nourishes qi, using qi to generate blood, replenishing yin, moistening dry blood and generating new blood, allowing rhubarb, ground beetle, and other herbs to exert their effects of dispelling blood stasis and breaking up accumulations; it also serves as an assistant herb.

[0046] Prepared licorice root is sweet and neutral in nature. It enters the heart, lung, spleen, and stomach meridians. It has the effects of tonifying the spleen and replenishing qi, moistening the lungs and relieving cough, relieving spasms and pain, and moderating the properties of other herbs. It is used for spleen and stomach weakness, insufficient qi, shortness of breath and fatigue, poor appetite and loose stools, carbuncles and boils, food or drug poisoning, and abdominal or limb spasms and pain. The *Shennong Bencao Jing* states that it is "sweet and neutral in nature. It treats cold and heat evil qi in the five viscera and six bowels, strengthens tendons and bones, promotes muscle growth, increases strength, treats metal wounds, and detoxifies. Long-term use lightens the body and prolongs life." The *Mingyi Bielu* states that it "unblocks the meridians, benefits blood and qi, and detoxifies various poisons." In this formula, the sweetness of licorice root harmonizes the other herbs and, combined with astragalus root, tonifies deficiency and generates blood.

[0047] The formula combines both purging and tonifying approaches, with both elimination and tonification, and the medicine is potent yet not drastic. The underlying pathogenesis of the entire formula is based on blood stasis and fluid retention, with internal accumulation of stagnant blood. The treatment principle is to invigorate blood circulation, break up accumulations, tonify deficiency, and resolve blood stasis.

[0048] Traditional Chinese medicine (TCM) considers microcirculation, or "blood stasis" in TCM, to be the site of blood circulation between arterioles and venules, where blood and tissue cells exchange substances. If the renal microcirculation is impaired, the kidneys' blood metabolism function declines, directly affecting their physiological function. The worse the microcirculation, the slower the blood flow, resulting in "traffic congestion." The kidneys' function is to expel turbid yin from the body. If kidney function declines, turbid yin is not expelled in time, stagnating in the body, causing blood stasis and fluid retention, which in turn hinders the normal functioning of the kidneys, leading to nephritis.

[0049] Traditional Chinese medicine (TCM) can improve renal microcirculation, alter glomerular filtration rate, and enhance blood circulation and detoxification, making it widely used for impaired kidney function. Extracts from *Eupolyphaga sinensis* (a type of worm) have antithrombotic and anticoagulant effects. Hirudin and 70% hirudin alcohol extract both have antithrombotic and thrombolytic effects; *Eupolyphaga sinensis* and leeches can also lower blood lipids. Rhubarb (processed with wine), *Eupolyphaga sinensis*, and other TCM herbs can combat hypercoagulability, regulate the balance of the thromboxane-prostaglandin system, improve microcirculation, improve blood rheological parameters, bidirectionally regulate intracellular cyclic nucleotides, scavenge free radicals, and reduce kidney damage caused by free radicals.

[0050] In summary, the present application provides a new traditional Chinese medicine composition, animal experiments show that the traditional Chinese medicine composition can effectively improve the microcirculation. The traditional Chinese medicine composition is used for improving the glomerular capillary microcirculation, which can effectively delay the progression of chronic nephritis, protect the kidney function, reduce the occurrence of complications, and improve the quality of life of patients. Therefore, the present application has good application prospect.

[0051] Obviously, according to the above content of the present application, according to the ordinary technical knowledge and common means in the art, other various forms of modification, replacement or change can be made without departing from the above technical idea of the present application.

[0052] The above content of the present application will be further described in detail through the following embodiment form. However, it should not be understood that the scope of the above subject matter of the present application is limited to the following examples. Any technology realized based on the above content of the present application belongs to the scope of the present application. DETAILED DESCRIPTION

[0053] In the following examples and experimental examples, the reagents and raw materials not specifically described are commercially available.

[0054] Example 1 Traditional Chinese medicine composition

[0055] The traditional Chinese medicine composition of the present embodiment is prepared by using the following raw materials:

[0056] Radix Paeoniae Alba 15g, Ganoderma lucidum 9g, Rhei Radix et Rhizoma Praeparata cum Pino 6g, Eupolyphaga 9g, Radix Astragali 12g, Bombyx Batryticatus 9g, Hirudo 6g, Radix Glycyrrhizae Praeparata cum Pino 6g.

[0057] The processing method of the Rhei Radix et Rhizoma Praeparata cum Pino is performed according to the standard of Chinese Pharmacopoeia, specifically: taking Rhei Radix et Rhizoma, mixing with yellow rice wine, soaking, frying, taking out, and cooling.

[0058] The preparation process is as follows: taking each medicine according to the prescription, adding 10 times the volume of water, decocting for 60min, decocting for 3 times, combining the decocting liquid, and concentrating to 2.4g crude drug / mL, to obtain the product.

[0059] In the following experiments, the traditional Chinese medicine composition prepared in the present embodiment is referred to as the present application.

[0060] Example 2 Traditional Chinese medicine composition

[0061] The traditional Chinese medicine composition of the present embodiment is prepared by using the following raw materials:

[0062] Radix Paeoniae Alba 10g, Ganoderma lucidum 14g, Rhei Radix et Rhizoma Praeparata cum Pino 1g, Eupolyphaga 14g, Radix Astragali 7g, Bombyx Batryticatus 14g, Hirudo 1g, Radix Glycyrrhizae Praeparata cum Pino 11g.

[0063] The preparation process of the present embodiment is the same as that of example 1.

[0064] The Chinese medicine composition of Example 3

[0065] The Chinese medicine composition of the present example is prepared by using the following raw materials:

[0066] Radix Paeoniae Alba 20g, Ganoderma lucidum 4g, Rhei Radix et Rhizoma 11g, Eupolyphaga 4g, Astragalus 17g, Bombyx Batryticatus 4g, Hirudo 11g, Radix Glycyrrhizae 1g.

[0067] The preparation process of the present example is the same as that of Example 1.

[0068] The Chinese medicine composition of Example 4

[0069] The Chinese medicine composition of the present example is prepared by using the following raw materials:

[0070] Radix Paeoniae Alba 16g, Ganoderma lucidum 8g, Rhei Radix et Rhizoma 7g, Eupolyphaga 8g, Astragalus 13g, Bombyx Batryticatus 8g, Hirudo 7g, Radix Glycyrrhizae 5g.

[0071] The preparation process of the present example is the same as that of Example 1.

[0072] The Chinese medicine composition of Example 5

[0073] The Chinese medicine composition of the present example is prepared by using the following raw materials:

[0074] Radix Paeoniae Alba 14g, Ganoderma lucidum 10g, Rhei Radix et Rhizoma 5g, Eupolyphaga 10g, Astragalus 11g, Bombyx Batryticatus 10g, Hirudo 5g, Radix Glycyrrhizae 7g.

[0075] The preparation process of the present example is the same as that of Example 1.

[0076] The technical scheme of the present application is further illustrated by experiments. In the following experiments, the de-protein-in-urine formula is prepared according to the method of Example 1.

[0077] Study on the effect of the Chinese medicine composition of Experimental Example 1 on the microcirculation of mouse ear

[0078] I. Experimental method

[0079] 1. Experimental grouping and administration

[0080] KM mice, 40, half male and female, 18-22g, were randomly divided into 5 groups: normal control group, positive drug group (drug and dose: aspirin enteric-coated tablets 250mg / kg), low and high dose groups of urine protein removing recipe (drug and dose: 10, 20 times of the clinical dose). The positive control group was given aspirin enteric-coated tablets with a concentration of 25mg / mL, and the low and high dose groups of the present recipe were given the extract of the present recipe with a concentration of 1.2g crude drug / mL and 2.4g crude drug / mL, respectively; the gavage volume was 0.1ml / 10g body weight. The normal control group was given distilled water, and the gavage volume was 0.1mL / 10g body weight. Once a day, continuous gavage for 3 days, 1h after administration in the morning of the last day (12h fasting without water), the experiment started.

[0081] Effect on the ear microcirculation of normal mice

[0082] The mice were anesthetized by intraperitoneal injection of chloral hydrate (350mg / kg), the mouse hair on the ear was removed with medical rubber cement, the anesthetized mice were fixed on the mouse observation table with the abdomen down, the ear support height was adjusted to make the mouse's auricle spread flat on the ear support, and 1-2 drops of camphor oil were added on the auricle, then the observation table was placed on the microscope stage, and the changes of ear microcirculation of mice in each group before and after administration were observed under transmitted light, including the diameter of auricular arteriole, the diameter of auricular venule, the number of vascular cross and the amount of capillary opening.

[0083] The test results were statistically analyzed by SPSS22 software, and the differences between groups were analyzed by one-way ANOVA.

[0084] II. Experimental results

[0085] 1. Effect on the diameter of auricular venule of normal mice

[0086] As shown in Table 1, after the normal mice were given the extract of the present recipe by gavage, the diameter of auricular venule increased with the increase of dose and the extension of time, and there was a statistical difference before and after administration (P<0.05, P<0.01); compared with the blank group, the aspirin enteric-coated tablets group and the high and low dose groups of urine protein removing recipe had statistical differences in increasing the diameter of auricular venule and the change rate of auricular venule diameter (P<0.05).

[0087] Table 1 Effect of the present recipe on the diameter of auricular venule of normal mice (x±s, n=10)

[0088]

[0089] Note: compared with the self before administration: * P<0.05, ** P<0.01; compared with the blank group at the same time point: # P<0.05, ##P < 0.01.

[0090] 2. Effect on the ear arteriole diameter of normal mice

[0091] As shown in Table 2, the ear arteriole diameter of normal mice increased with the increase of the dose and the extension of time after the oral administration of the extract of the present application, and there was a statistical difference (P < 0.01) compared with that before administration. Compared with the blank group, the low and high dose groups of the present application and the aspirin enteric-coated tablets had statistical differences (P < 0.01, P < 0.05) in increasing the ear arteriole diameter and the arteriole diameter change rate.

[0092] Table 2 Effect of the present application on the ear arteriole diameter of normal mice (x ± s, n = 10)

[0093]

[0094] Note: Compared with the self before administration: * P < 0.05, ** P < 0.01; compared with the blank group at the same time point: # P < 0.05, ## P < 0.01.

[0095] 3. Effect on the ear capillary open number of normal mice

[0096] As shown in Table 3, the statistical number of the ear capillary open number also increased with the increase of the dose and the extension of time compared with that before administration, and there was a statistical difference (P < 0.05, P < 0.01). Compared with the blank group, the aspirin enteric-coated tablet group and the low and high dose groups of the present application had statistical differences (P < 0.05, P < 0.01) in the number of the ear capillary.

[0097] Table 3 Effect of the present application on the ear capillary open number of normal mice (x ± s, n = 10)

[0098]

[0099] Note: Compared with the self before administration: * P < 0.05, ** P < 0.01; compared with the blank group at the same time point: # P < 0.05, ## P < 0.01.

[0100] 4. Effect on the ear capillary open number of normal mice

[0101] As seen from Table 4, compared with before administration, the statistical number of auricle capillary cross number increases with the increase of dose and the extension of time, and has statistical difference (P<0.05, P<0.01); compared with the blank group, the change of auricle capillary cross number counted by the high-dose group of the prescription of the application has statistical difference (P<0.05, P<0.01).

[0102] Table 4 Effect of the prescription of the application on the auricle capillary cross number of normal mice (x±s, n=10)

[0103]

[0104] Note: compared with before administration by itself: * P<0.05, ** P<0.01; compared with the blank group at the same time point: # P<0.05, ## P<0.01.

[0105] As can be seen from the above experimental results, the traditional Chinese medicine composition provided by the application can increase the diameter of capillary and arteriole, can increase the number of capillary opening amount, and can increase the number of capillary cross, therefore, the traditional Chinese medicine composition of the application has the effect of improving microcirculation.

[0106] As can be seen from the above examples and experimental examples, the application provides a new traditional Chinese medicine composition, which has the effect of improving microcirculation. The traditional Chinese medicine composition is used for improving the glomerular capillary microcirculation, can effectively delay the progression of chronic nephritis, protect the kidney function, reduce the occurrence of complications, and improve the life quality of patients. Therefore, the application has good application prospect.

Claims

1. A traditional Chinese medicine composition, characterized in that, It is made from the following ingredients by weight: 10-20 parts of white peony root 4-14 portions of Ganoderma lucidum 1-11 parts rhubarb 4-14 parts of ground beetle Astragalus membranaceus 7-17 parts 4-14 portions of silkworm pupae Leeches 1-11 parts Prepared licorice root, 1-11 parts.

2. The traditional Chinese medicine composition according to claim 1, characterized in that, It is made from the following ingredients by weight: 14-16 parts of white peony root 8-10 portions of Ganoderma lucidum 5-7 parts rhubarb 8-10 parts of ground beetle Astragalus membranaceus 11-13 parts 8-10 portions of silkworm pupae Leeches 5-7 parts Prepared licorice root, 5-7 parts.

3. The traditional Chinese medicine composition according to claim 2, characterized in that, It is made from the following ingredients by weight: 15 parts of white peony root 9 portions of Ganoderma lucidum 6 parts rhubarb 9 portions of ground beetle 12 portions of Astragalus membranaceus 9 portions of silkworm pupae 6 portions of leeches Prepared licorice root, 6 parts.

4. The traditional Chinese medicine composition according to any one of claims 1-3, characterized in that: The rhubarb mentioned is wine-processed rhubarb.

5. The traditional Chinese medicine composition according to any one of claims 1-3, characterized in that: It is a formulation prepared by adding pharmaceutically acceptable excipients or auxiliary ingredients to the active ingredients of the raw material's original powder, water or organic solvent extract.

6. The traditional Chinese medicine composition according to claim 5, characterized in that: The preparations are granules, pills, powders, decoctions, tablets, capsules, solutions, or spray-dried powders.

7. Use of the traditional Chinese medicine composition according to any one of claims 1-6 in the preparation of a medicament for improving microcirculation.

8. The use according to claim 9, characterized in that: The drug is used to improve glomerular capillary microcirculation.

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