Application of feruloyl putrescine in preparation of deoxynivalenol
By adding ferulic acid putrescine to the culture medium, the expression of the TRI gene and the formation of toxin-producing bodies in Fusarium graminearum were promoted, which solved the problem of unstable DON preparation and achieved efficient and stable DON preparation, meeting the needs of large-scale and standardized production.
Patent Information
- Application Number
- CN202511713860.4
- Authority / Receiving Office
- CN · China
- Patent Type
- Applications(China)
- Current Assignee / Owner
- Filing Date
- 2025-11-21
- Publication Date
- 2025-12-19
- Estimated Expiration
- 2045-11-21
AI Technical Summary
Existing technologies are insufficient for the efficient and stable preparation of deoxynivalenol (DON) under controlled conditions. Furthermore, traditional methods are costly and have unstable yields, making it difficult to meet the needs of large-scale and standardized applications.
Feruloyl putrescine was used as a culture medium additive to promote the expression of TRI genes in Fusarium graminearum, especially TRI1, TRI5 and TRI12 genes, to form toxin-producing bodies. The liquid toxin-producing culture medium formulation was optimized, with the concentration of feruloyl putrescine ranging from 30 μM to 300 μM.
This study achieved stable and significantly increased DON production, eliminating dependence on crop growth cycles and contamination environments, providing a reliable guarantee for the standardized and large-scale preparation of DON, and revealing the deep mechanism of action of ferulic acid putrescine on DON synthesis.