Bacillus subtilis gl57 and application of rain-resistant bacterial agent

By using Bacillus subtilis GL57 and its prepared rain-resistant bacterial agent, the problems of resistance and colonization of maize leaf spot disease controlled by chemical agents have been solved, achieving highly efficient biological control in areas with frequent rainfall, and improving maize yield and health.

CN121320152BActive Publication Date: 2026-06-02INSTITUTE OF ENVIRONMENT AND SUSTAINABLE DEVELOPMENT IN AGRICULTURE CAAS
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Patents(China)
Current Assignee / Owner
INSTITUTE OF ENVIRONMENT AND SUSTAINABLE DEVELOPMENT IN AGRICULTURE CAAS
Filing Date
2025-10-17
Publication Date
2026-06-02

AI Technical Summary

Technical Problem

Existing chemical methods for controlling maize leaf spot have problems such as increased pathogen resistance, damage to soil microbial communities, and pesticide residues. Furthermore, biocontrol bacteria are difficult to colonize under rainwater washout, affecting the control effect.

Method used

Using Bacillus subtilis GL57 and its prepared rain-resistant bacterial agent, the colonization ability of the bacterial agent under rainwater runoff was improved by compounding xanthan gum, chitosan and diatomaceous earth. Combined with fermentation process optimization, a bacterial agent with significant antibacterial activity was prepared.

Benefits of technology

In areas with frequent rainfall, it significantly improved the colonization rate and control effect of biocontrol bacteria on leaves, reduced the disease index, and improved the agronomic traits and yield of maize.

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Abstract

The application discloses a bacillus subtilis GL57, rain erosion resistant bacterial agent and application thereof, and belongs to the technical field of agricultural microorganisms. The preservation number of the bacillus subtilis GL57 is CGMCC No. 35080. It is found through verification that the strain GL57 has excellent inhibiting effect on dicladiodendron communes, and the strain GL57 has bacteriostatic activity. The bacterial agent is obtained through a two-stage temperature control method combined with a carrier complex rain erosion resistant agent process. In a field condition, the GL57 rain erosion resistant bacterial agent is applied to corn leaves, and has a significant biological control effect on corn small spot, and it is indicated that the GL57 and the bacterial agent provided by the application have good application potential and prospect in the prevention and treatment of gramineous plant diseases in a corn small spot epidemic area caused by frequent rainfall.
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