Construction method of soybean GmLNK2 gene four mutants and application of soybean GmLNK2 gene four mutants in soybean disease-resistant breeding

The four mutants gmlnk2-4m of the soybean GmLNK2 gene were constructed using CRISPR/Cas9 technology, solving the problem of breeding resistance to soybean root rot, significantly improving soybean resistance to root rot, and providing candidate gene resources for disease resistance breeding.

CN121495985APending Publication Date: 2026-02-10HENAN UNIVERSITY
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Patent Information

Application Number
CN202512019781.X
Authority / Receiving Office
CN · China
Patent Type
Applications(China)
Current Assignee / Owner
Filing Date
2025-12-30
Publication Date
2026-02-10

AI Technical Summary

Technical Problem

Existing technologies are insufficient to effectively address the problem of soybean root rot, especially the challenge of precisely regulating the expression or function of soybean disease resistance-related genes through gene editing technology to cultivate new disease-resistant varieties.

Method used

Using CRISPR/Cas9 gene editing technology, three specific targets were designed targeting the soybean GmLNK2a, GmLNK2b, GmLNK2c, and GmLNK2d genes. A four-mutant of the soybean GmLNK2 gene, gmlnk2-4m, was constructed. The editing vector was introduced into soybean plants using Agrobacterium-mediated transformation to obtain a homozygous four-mutant, which significantly improved resistance to root rot.

Benefits of technology

The key role of the GmLNK2 gene family in soybean resistance to root rot was clarified. The mutant plants were more sensitive to the root rot pathogen and their growth and development were inhibited. This provides candidate gene resources for disease resistance breeding and enhances the disease resistance of soybean.

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Abstract

The invention belongs to the technical field of plant genetic engineering and disease-resistant breeding, and particularly relates to a construction method of soybean GmLNK2 gene four mutants and application of the soybean GmLNK2 gene four mutants in soybean disease-resistant breeding, by means of a CRISPR / Cas9 technology, wild soybean WS82 is used as a material, three target spots are designed for GmLNK2a, GmLNK2b, GmLNK2c and GmLNK2d genes of a GmLNK2 gene family, and simultaneous knockout is performed to obtain a mutant plant. Compared with a wild type WS82, the plant height of a mutant plant is obviously reduced, the length of a main root is obviously shortened, and the leaf area of three-out compound leaves is obviously reduced, which indicates that the mutant plant is more sensitive to pathogenic bacteria in a root rot disease nursery, the susceptibility degree is more serious, and growth and development are obviously inhibited, the key effect of the GmLNK2 gene family in soybean root rot resistance is clarified, and the application of the GmLNK2 gene family in soybean root rot resistance is developed. Important gene resources and technical support are provided for soybean disease-resistant molecular mechanism research and disease-resistant variety cultivation.
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