Method for detecting prostate cancer marker GSTP1 hypermethylation promoter DNA in urine

By modifying a bridge-shaped tetrahedral DNA probe onto a liquid-gate graphene transistor sensor, GSTP1 hypermethylation in urine can be identified, solving the sensitivity and cost problems of urine detection in existing technologies and realizing efficient, portable, and non-invasive diagnosis of prostate cancer.

CN121629045APending Publication Date: 2026-03-10HUBEI UNIV
0 Cites 0 Cited by

Patent Information

Application Number
CN202411234351.9
Authority / Receiving Office
CN · China
Patent Type
Applications(China)
Current Assignee / Owner
Filing Date
2024-09-04
Publication Date
2026-03-10

AI Technical Summary

Technical Problem

Existing technologies are insufficient for the detection of highly sensitive, rapid, low-cost, and portable GSTP1 hypermethylated promoter DNA in urine for prostate cancer, hindering the clinical translation of non-invasive prostate cancer diagnosis.

Method used

A liquid-gate graphene transistor sensor is used. By modifying the gate surface with a bridge-shaped tetrahedral DNA probe, the hypermethylation of the GSTP1 promoter after bisulfite treatment is identified. The high carrier mobility and biocompatibility of graphene are utilized to detect methylated DNA in urine and realize electrical signal conversion.

Benefits of technology

It achieves a detection limit as low as 10-18M, a wide linear detection range, is suitable for complex artificial urine environments and clinical samples, is simple and convenient to operate, has a fast detection time, and possesses high sensitivity and good stability.

✦ Generated by Eureka AI based on patent content.
Patent Text Reader

Abstract

The invention provides a method for rapidly and sensitively detecting GSTP1 (Glutathione S Transferase Protein 1) hypermethylated promoter DNA (Deoxyribose Nucleic Acid) of prostatic cancer in urine. Based on the liquid gate graphene transistor sensor, a bridge-shaped tetrahedral DNA probe is modified to the surface of a gold gate through a gold-sulfur bond, and a hypermethylated GSTP1 promoter converted by hydrosulphite can be selectively captured. Wherein DNA is treated by hydrosulfite, so that unmethylated cytosine (C) can be converted into uracil (U), and methylated cytosine (5-mC) is kept unchanged. According to the bridge-shaped tetrahedron DNA, due to the influence of electrostatic repulsion and space structure between two tetrahedrons, the transverse distance between curled ssDNA is easily controlled to improve the hybridization efficiency and reduce Debye shielding, the detection limit can be as low as 10-18 M, the wide linear detection range of 10 <-18 >-10 <-8 > M can be achieved, and the detection efficiency is greatly improved. The kit has a good detection effect in complex artificial urine and clinical practical sample detection, can effectively distinguish clinical urine samples of prostatic cancer patients and control groups, and has clinical application value.
Need to check novelty before this filing date? Find Prior Art