A composition for anti-aging, brightening and shrinking pores and its use

Abstract

The present application relates to a kind of anti-aging composition and its application of brightening and shrinking pores.The composition includes the following components: halocyprid extract, purple magnolia extract, desert rose leaf cell extract, rain lily labiatae flower extract and white lily extract, and the mass ratio of the halocyprid extract, purple magnolia extract, desert rose leaf cell extract, rain lily labiatae flower extract and white lily extract is (0.2-2):(0.001-0.1):(0.001-0.1):(0.001-0.1):(0.001-0.1).The composition of the present application can effectively improve the clearance rate of collagenase, maintain the elasticity and tightness of skin, have good anti-aging and pore-shrinking effect, and can also effectively remove hydroxyl radicals, have good whitening and brightening effect.

Description

Technical Field

[0001] This invention relates to the technical field of cosmetics, specifically to an anti-aging, brightening, and pore-minimizing composition and its application. Background Technology

[0002] Traditional cosmetic compositions have long faced the bottleneck of limited efficacy, stemming from the simple stacking of active ingredients and the lack of synergistic mechanisms. Most cosmetics on the market today achieve basic efficacy by simply mixing various single-function ingredients. However, the differences in physicochemical properties between different ingredients can easily lead to the loss of active ingredients or antagonistic interactions, significantly diminishing the claimed "multi-effect" effect and often resulting in only localized benefits. For example, temperature-sensitive ingredients like Vitamin C and retinol are difficult to stabilize and absorb efficiently through the skin in conventional formulations, thus affecting the longevity of their effects. Furthermore, current technology faces the challenge of overcoming the compatibility barriers between water-soluble ingredients (such as hyaluronic acid) and fat-soluble ingredients (such as squalane), which may prevent the simultaneous achievement of multiple effects such as anti-oxidation, repair, and anti-aging.

[0003] Currently, some technical solutions have attempted to incorporate artemia extract to address this issue. The active ingredients in artemia extract include GP4G, proteins, amino acids, unsaturated fatty acids, vitamins, and minerals. Among these, GP4G and stress-relief proteins (such as HSP70) can enhance cellular energy metabolism and possess antioxidant capabilities, helping to eliminate free radicals in the skin, slow down the skin aging process, and prevent wrinkles and sagging. However, the extraction of artemia extract often employs a high-speed shear-ultrasound coupling process. If temperature control is inadequate, GP4G is prone to ring-opening degradation, and stress-relief proteins may denature, thus affecting the quality and efficacy of the resulting product. Summary of the Invention

[0004] The purpose of this invention is to overcome the shortcomings of the prior art and provide a composition for anti-aging, brightening, and shrinking pores, as well as its application.

[0005] To achieve the above objectives, the technical solution adopted by the present invention is as follows:

[0006] In a first aspect, the present invention provides an anti-aging, brightening, and pore-shrinking composition, comprising the following components: Artemia extract, Magnolia liliiflora extract, Rosa desertica leaf cell extract, Lilium lancifolium orchid extract, and Lilium lancifolium extract, wherein the mass ratio of Artemia extract, Magnolia liliiflora extract, Rosa desertica leaf cell extract, Lilium lancifolium orchid extract, and Lilium lancifolium extract is (0.2-2):(0.001-0.1):(0.001-0.1):(0.001-0.1):(0.001-0.1).

[0007] The preparation method of the brine shrimp extract includes the following steps:

[0008] S1. Select fresh brine shrimp that have been washed and drained, add 3-4 times the weight of deionized water to make a pulp, and then add alkaline protease to the brine shrimp pulp for enzymatic hydrolysis, inactivate the enzyme and sterilize to obtain the hydrolysate.

[0009] S2. Inoculate the enzymatic hydrolysate with a mixed bacterial culture of Bacillus coagulans, Lactobacillus johnsonii and Saccharomyces rouxii, and ferment at 36-38℃ for 48-60 h to obtain the fermentation product;

[0010] S3. The fermentation product is filtered to remove bacteria, and the filtrate is freeze-dried under vacuum to obtain the Artemia extract.

[0011] The preparation method of the brine shrimp extract in the composition of the present invention solves the problem of the loss of activity of active ingredients in existing brine shrimp extracts during the extraction process by enzymatic hydrolysis, mixed fermentation, and vacuum freeze-drying, as detailed below:

[0012] In step S1 of the preparation of Artemia extract, alkaline protease is used to enzymatically hydrolyze Artemia, which can hydrolyze large molecules such as stress-resistant proteins into smaller, more water-soluble active peptides. In step S2 of the preparation of Artemia extract, mixed fermentation of Bacillus coagulans, Lactobacillus johnsonii, and Saccharomyces rouxii is carried out in the hydrolysate to generate metabolites such as polysaccharides. These metabolites can form complexes with active peptides through hydrogen bonds, reducing the risk of thermal denaturation. In addition, L-lactic acid generated by Lactobacillus johnsonii can lower the pH of the system, thereby reducing the alkaline-catalyzed hydrolysis of GP4G. In step S3 of the preparation of Artemia extract, vacuum freeze-drying is used to avoid the destruction of GP4G and active peptides by high temperature.

[0013] The functions of each component in the composition of the present invention are as follows:

[0014] Artemia extract contains small molecule active peptides, GP4G, and other components. Small molecule active peptides can promote collagen synthesis, increase skin elasticity, and tighten the skin around pores. GP4G can enhance cellular energy metabolism and improve mitochondrial respiration by activating AMPK / PGC-1α, indirectly accelerating the barrier repair rate. Artemia extract can also reduce UVB-induced ROS, achieving antioxidant effects.

[0015] The main components of magnolia extract are magnolin and flavonoids, which can inhibit tyrosinase activity, reduce melanin production, and brighten the skin. They can also protect cell DNA and protein structure, promote collagen synthesis to increase elasticity, reduce wrinkles, and prevent pore enlargement.

[0016] The main components of desert rose leaf cell extract are polysaccharides, flavonoids, and phenolic acids, which can enhance cell resistance, improve skin's tolerance to the external environment, promote metabolism, accelerate the shedding of aging cells and the generation of new cells. Among them, polysaccharides have a moisturizing effect, which can increase skin moisture content, improve water-oil balance, prevent dull skin tone, and improve dryness and roughness caused by enlarged pores.

[0017] The main components of the extract of Lilium lancifolium are alkaloids, polysaccharides, and amino acids, which can inhibit the activity of matrix metalloproteinases (MMPs), reduce collagen degradation, promote stratum corneum metabolism, and repair the skin barrier.

[0018] The main components of white lily extract are lily polysaccharides, saponins, and vitamin C derivatives. It can inhibit melanin transport mediated by protease-activated receptor-2 (PAR-2), reduce epidermal melanin deposition, and brighten the skin. Its polysaccharide and saponin components can promote the renewal of the stratum corneum, help the normal shedding of aged keratinocytes, and prevent pores from becoming clogged. At the same time, the extract has good moisturizing and antioxidant properties, which can improve dry and rough skin and enhance its luster and softness.

[0019] Preferably, the mass ratio of the Artemia extract, Magnolia liliiflora extract, Rosa desertica leaf cell extract, Lilium lancifolium orchid extract and Lilium lancifolium extract is (0.6-1.5):(0.02-0.05):(0.05-0.07):(0.03-0.08):(0.005-0.02).

[0020] Preferably, in step S1 of preparing the artichoke extract, the amount of alkaline protease added is 2000-3000 U / g of artichoke mass, the enzymatic hydrolysis temperature is 40-50℃, the pH is 8.5-9.0, and the time is 40-60 min; the enzyme inactivation sterilization is carried out by microwave enzyme inactivation sterilization, the microwave power is 300W-800W, and the time is 5-10 min.

[0021] Preferably, in step S2 of preparing the artichoke extract, the inoculation amount of the mixed bacterial solution is 5-10% v / v of the enzymatic hydrolysate, and the total viable count of the mixed bacterial solution is 1.5 × 10⁻⁶. 8 -2×10 9 CFU / mL, the ratio of viable Bacillus coagulans, Lactobacillus johnsonii and Saccharomyces rouxii in the mixed bacterial solution is 1:(0.1-0.5):(1.5-3).

[0022] Preferably, in step S3 of preparing the Artemia extract, the filtration sterilization involves filtering the fermentation product through a 0.22 μm filter membrane for sterilization.

[0023] In a second aspect, the present invention provides the application of the anti-aging, brightening, and pore-minimizing composition of the first aspect in the preparation of cosmetics, wherein the cosmetics are toners, creams, masks, serums, or sprays, and the amount of the composition added is 0.5%-3% of the total mass of the cosmetics.

[0024] Thirdly, the present invention provides an essence comprising the following ingredients by weight percentage: 0.5%-3% of the anti-aging, brightening, and pore-minimizing composition of the first aspect, 0.1%-1% of a moisturizer, 0.3%-3.6% of a skin conditioning agent, 0.05%-0.5% of a thickener, 0.05%-0.1% of a preservative, and 0.01%-0.3% of a pH adjuster, with the balance being deionized water.

[0025] Preferably, the moisturizer is at least one selected from allantoin, betaine, β-glucan, trehalose, glycerin, and sodium hyaluronate.

[0026] Preferably, the skin conditioning agent is at least one of dipeptide-15, ribose, nicotinamide adenine dinucleotide, and trisodium fructose diphosphate.

[0027] Preferably, the thickener is xanthan gum and / or carbomer.

[0028] Preferably, the preservative is 1,2-hexanediol and / or p-hydroxyacetophenone.

[0029] Preferably, the pH adjuster is arginine and / or tromethamine.

[0030] Preferably, the skin conditioning agent comprises the following raw materials in weight percentages: dipeptide-15 0.1%-1.5%, ribose 0.2%-2%, nicotinamide adenine dinucleotide 0.003%-0.03%, and trisodium fructose diphosphate 0.001-0.01%.

[0031] Fourthly, the present invention provides a method for preparing the essence in the third aspect, comprising the following steps:

[0032] (1) Mix the humectant, thickener and part of deionized water, homogenize at 75-85℃, add the preservative, stir evenly to obtain a mixture;

[0033] (2) When the temperature of the mixture in S1 drops to 35-45℃, add the components of the anti-aging, brightening and shrinking pore composition, skin conditioning agent, remaining deionized water and pH adjuster, stir evenly to obtain the essence.

[0034] Compared with the prior art, the beneficial effects of the present invention are as follows:

[0035] (1) In the preparation method of Artemia extract, the present invention solves the problem of the damage to the activity of active ingredients in existing Artemia extract during the extraction process by adopting enzymatic hydrolysis + mixed bacterial fermentation + vacuum freeze drying, and prepares Artemia extract with antioxidant, anti-aging and repair effects.

[0036] (2) When the Artemia extract obtained in this invention is combined with Magnolia lilii extract, Rosa desertica leaf cell extract, Lilium lancifolium extract and Lilium lancifolium extract in the composition of this invention, it can effectively improve the collagenase clearance rate, maintain the elasticity and firmness of the skin, and play a good anti-aging and pore shrinking effect. It can also effectively remove hydroxyl free radicals and has a good whitening and brightening effect. Detailed Implementation

[0037] To better illustrate the purpose, technical solution, and advantages of the present invention, the present invention will be further described below in conjunction with specific embodiments.

[0038] The sources used in the following embodiments and comparative examples are as follows:

[0039] Artemia: from Qixiangcuo Salt Lake in Shuanghu County, Nagqu City, Tibet Autonomous Region;

[0040] Bacillus coagulans: purchased from China General Microbiological Culture Collection Center, accession number CGMCC1.10823;

[0041] Lactobacillus johnsonii: purchased from China General Microbiological Culture Collection Center, accession number CGMCC 1.3260;

[0042] Saccharomyces rouxii: purchased from China General Microbiological Culture Collection Center, accession number CGMCC 2.2926;

[0043] Magnolia liliiflora extract: Manufacturer: Xinyang Mufan Biotechnology Co., Ltd., Model: MF-007111;

[0044] Desert Rose Leaf Cell Extract: Manufacturer: Beijing Lingbao Technology Co., Ltd., Product No.: P0123401;

[0045] Power Plus Peru Orchid W Extract: Manufacturer: Shanghai Shengyu Meike Biotechnology Co., Ltd., trade name: Peruvian Black Orchid Cell Culture;

[0046] White lily extract: The manufacturer is Shanghai Shengyu Meike Biotechnology Co., Ltd., and the product name is white lily cell culture.

[0047] Unless otherwise specified, all other materials and reagents used in the examples are commercially available.

[0048] Examples 1-5 and Comparative Examples 1-5

[0049] Specifically, the preparation method of the brine shrimp extract includes the following steps:

[0050] S1. Select fresh brine shrimp that have been washed and drained, add 3.5 times their weight of deionized water to make a pulp, and then add alkaline protease to the brine shrimp pulp for enzymatic hydrolysis, followed by enzyme inactivation and sterilization to obtain the enzymatic hydrolysate; wherein, the amount of alkaline protease added is 25000 U / g brine shrimp mass, the enzymatic hydrolysis temperature is 45℃, the pH is 8.5, and the time is 45 min; the enzyme inactivation and sterilization are carried out by microwave inactivation and sterilization, with a microwave power of 600W and a time of 8 min;

[0051] S2. Inoculate the enzymatic hydrolysate with a mixed bacterial culture of Bacillus coagulans, Lactobacillus johnsonii, and Saccharomyces rouxii, and ferment at 37°C for 56 hours to obtain the fermentation product; wherein the inoculation amount of the mixed bacterial culture is 8% v / v of the enzymatic hydrolysate, and the total viable count of the mixed bacterial culture is 1×10⁻⁶. 9 CFU / mL, the ratio of viable Bacillus coagulans, Lactobacillus johnsonii, and Saccharomyces rouxii in the mixed bacterial solution is 1:0.3:2.4;

[0052] S3. The fermentation product is filtered through a 0.22μm filter membrane for sterilization, and the filtrate is freeze-dried under vacuum to obtain the Artemia extract.

[0053] The components and their masses of the anti-aging, brightening and pore-shrinking compositions of Examples 1-5 and Comparative Examples 1-5 are shown in Table 1. The total mass of each composition is equal.

[0054] The preparation method of the anti-aging, brightening, and pore-shrinking compositions of Examples 1-5 and Comparative Examples 1-5 includes the following steps:

[0055] Mix all components thoroughly to obtain the anti-aging, brightening, and pore-shrinking composition.

[0056] Table 1. Components and their mass ratios in the anti-aging, brightening, and pore-minimizing compositions of each group.

[0057]

[0058] Example 6

[0059] The only difference between Example 6 and Example 1 is that the preparation method of the brine shrimp extract includes the following steps:

[0060] S1. Select fresh brine shrimp that have been washed and drained, add 3 times their weight of deionized water to make a pulp, and then add alkaline protease to the brine shrimp pulp for enzymatic hydrolysis, followed by enzyme inactivation and sterilization to obtain the enzymatic hydrolysate; wherein, the amount of alkaline protease added is 2000 U / g brine shrimp mass, the enzymatic hydrolysis temperature is 50℃, the pH is 8.5, and the time is 60 min; the enzyme inactivation and sterilization are carried out by microwave inactivation and sterilization, with a microwave power of 300W and a time of 10 min;

[0061] S2. A mixed bacterial culture of Bacillus coagulans, Lactobacillus johnsonii, and Saccharomyces rouxii is inoculated into the enzymatic hydrolysate, and fermented at 36°C for 60 hours to obtain the fermentation product; wherein the inoculation amount of the mixed bacterial culture is 5% v / v of the enzymatic hydrolysate, and the total viable count of the mixed bacterial culture is 1.5 × 10⁻⁶. 8 CFU / mL, wherein the live count ratio of Bacillus coagulans, Lactobacillus johnsonii, and Saccharomyces rouxii in the mixed bacterial solution is 1:0.1:1.5;

[0062] S3. The fermentation product is filtered through a 0.22μm filter membrane for sterilization, and the filtrate is freeze-dried under vacuum to obtain the Artemia extract.

[0063] Example 7

[0064] The only difference between Example 7 and Example 1 is that the preparation method of the brine shrimp extract includes the following steps:

[0065] S1. Select fresh brine shrimp that have been washed and drained, add 4 times their weight of deionized water to make a pulp, and then add alkaline protease to the brine shrimp pulp for enzymatic hydrolysis, followed by enzyme inactivation and sterilization to obtain the enzymatic hydrolysate; wherein, the amount of alkaline protease added is 3000 U / g brine shrimp mass, the enzymatic hydrolysis temperature is 40℃, the pH is 9.0, and the time is 40 min; the enzyme inactivation and sterilization are carried out by microwave inactivation and sterilization, with a microwave power of 800W and a time of 5 min;

[0066] S2. Inoculate the enzymatic hydrolysate with a mixed bacterial culture of Bacillus coagulans, Lactobacillus johnsonii, and Saccharomyces rouxii, and ferment at 38°C for 48 hours to obtain the fermentation product; wherein the inoculation amount of the mixed bacterial culture is 10% v / v of the enzymatic hydrolysate, and the total viable count of the mixed bacterial culture is 2 × 10⁻⁶. 9 CFU / mL, the ratio of viable Bacillus coagulans, Lactobacillus johnsonii and Saccharomyces rouxii in the mixed bacterial solution is 1:0.5:3;

[0067] S3. The fermentation product is filtered through a 0.22μm filter membrane for sterilization, and the filtrate is freeze-dried under vacuum to obtain the Artemia extract.

[0068] Example 8

[0069] The only difference between Example 8 and Example 1 is that in step S2 of preparing the Artemia extract, the ratio of viable Bacillus coagulans, Lactobacillus johnsonii, and Saccharomyces rouxii in the mixed bacterial solution is 0.3:1:2.4.

[0070] Example 9

[0071] The only difference between Example 9 and Example 1 is that in step S2 of preparing the Artemia extract, the ratio of viable Bacillus coagulans, Lactobacillus johnsonii, and Saccharomyces rouxii in the mixed bacterial solution is 2.4:0.3:1.

[0072] Example 10

[0073] The only difference between Example 10 and Example 1 is that in step S2 of preparing the Artemia extract, the ratio of viable Bacillus coagulans, Lactobacillus johnsonii, and Saccharomyces rouxii in the mixed bacterial solution is 1:2.4:0.3.

[0074] Comparative Example 7

[0075] The only difference between Comparative Example 7 and Example 1 is that the preparation method of the artemia extract in Comparative Example 7 includes the following steps:

[0076] S1. Select fresh brine shrimp that have been washed and drained, add 3.5 times their weight of deionized water to make a pulp, and then add alkaline protease to the brine shrimp pulp for enzymatic hydrolysis, followed by enzyme inactivation and sterilization to obtain the enzymatic hydrolysate; wherein, the amount of alkaline protease added is 25000 U / g brine shrimp mass, the enzymatic hydrolysis temperature is 55℃, the pH is 8.5, and the time is 45 min; the enzyme inactivation and sterilization are carried out by microwave inactivation and sterilization, with a microwave power of 600W and a time of 8 min;

[0077] S2. Filter the enzymatic hydrolysate through a 0.22 μm filter membrane, and freeze-dry the filtrate under vacuum to obtain the Artemia extract.

[0078] Comparative Example 8

[0079] The only difference between Comparative Example 8 and Example 1 is that in step S2 of preparing the Artemia extract in Comparative Example 8, instead of inoculating with Bacillus coagulans, a mixed bacterial culture of Lactobacillus johnsonii and Saccharomyces rouxii was inoculated, and fermentation was carried out at 37°C for 56 hours to obtain the fermentation product; wherein, the inoculation amount of the mixed bacterial culture was 8% v / v of the enzymatic hydrolysate, and the total viable count of the mixed bacterial culture was 1×10⁻⁶. 9 The CFU / mL ratio of viable Lactobacillus johnsonii to Saccharomyces rouxii in the mixed bacterial solution was 0.3:2.4.

[0080] Comparative Example 9

[0081] The only difference between Comparative Example 9 and Example 1 is that in step S2 of preparing the Artemia extract in Comparative Example 9, instead of inoculating with Lactobacillus johnsonii, a mixed bacterial culture of Bacillus coagulans and Saccharomyces rouxii is inoculated, and fermentation is carried out at 37°C for 56 hours to obtain the fermentation product; wherein, the inoculation amount of the mixed bacterial culture is 8% v / v of the enzymatic hydrolysate, and the total viable count of the mixed bacterial culture is 1×10⁻⁶. 9 The CFU / mL ratio of viable Bacillus coagulans to Saccharomyces rouxii in the mixed bacterial solution is 1:2.4.

[0082] Comparative Example 10

[0083] The only difference between Comparative Example 10 and Example 1 is that in step S2 of preparing the Artemia extract in Comparative Example 10, instead of inoculating with *Saccharomyces rouxii*, a mixed bacterial culture of *Bacillus coagulans* and *Lactobacillus johnsonii* was inoculated, and fermentation was carried out at 37°C for 56 hours to obtain the fermentation product; wherein, the inoculation amount of the mixed bacterial culture was 8% v / v of the enzymatic hydrolysate, and the total viable count of the mixed bacterial culture was 1 × 10⁻⁶. 9 The CFU / mL ratio of viable Bacillus coagulans to Lactobacillus johnsonii in the mixed bacterial solution is 1:0.3.

[0084] Performance testing

[0085] Test Example 1: Test of the composition's ability to scavenge hydroxyl radicals

[0086] The testing of the composition's ability to scavenge hydroxyl radicals includes the following steps:

[0087] First, the compositions of Examples 1-10 and Comparative Examples 1-10 were diluted with deionized water to prepare a test sample solution with a mass concentration of 2%.

[0088] 1 mL of 0.4 mmol / L crystal violet, 3 mL of 0.2 mol / L pH 7.4 phosphate buffer, 1 mL of 0.001 mol / L FFeSO4 solution, and 1 mL of the test sample solution were added to a 10 mL centrifuge tube, respectively. Finally, 1 mL of 30% H2O2 solution was added to initiate the reaction. After reacting at room temperature for 1 hour, the mixture was centrifuged at 12000 rpm for 5 minutes. The absorbance of the supernatant at 510 nm was recorded as C. The absorbance when distilled water was used instead of the test sample solution was recorded as C1. The absorbance when distilled water was used instead of both the test sample solution and the H2O2 solution was recorded as C2. The average value was taken from three parallel operations. The scavenging rate of hydroxyl radicals was calculated as (C - C1) / (C2 - C1). The data are shown in Table 2. Hydroxyl radicals are the only free radicals that can directly trigger the production of melanin in the skin. The higher the scavenging rate of hydroxyl radicals, the better the whitening and brightening effect of the composition.

[0089] Test Example 2: Test on the Inhibition of Collagenase Activity by the Composition

[0090] The test for the composition's inhibition of collagenase activity includes the following steps:

[0091] Preparation of test sample solution: The compositions of Examples 1-10 and Comparative Examples 1-10 were mixed with Tris-HCl buffer (pH = 7.5 ± 0.2, containing 5 mmol / mL CaCl2) to prepare a 1 mg / mL test sample solution;

[0092] Preparation of positive control solution: Tetracycline hydrochloride was mixed with Tris-HCl buffer (pH = 7.5 ± 0.2, containing 5 mmol / mL CaCl2) to prepare a 1 mg / mL positive control solution;

[0093] Preparation of collagenase solution: Mix type I collagenase with Tris-HCl buffer (pH = 7.5 ± 0.2, containing 5 mmol / mL CaCl2) to prepare type I collagenase Tris-HCl solution, which is the collagenase solution;

[0094] Substrate solution preparation: Prepare a 0.5 mg / mL solution by mixing the substrate FALGPA with Tris-HCl buffer (pH = 7.5 ± 0.2, containing 5 mmol / mL CaCl2);

[0095] The following were set up: blank group, control group, sample group, and sample blank group. The specific feeding content is as follows:

[0096] Sample blank group: 60 μL of test sample solution + 140 μL of Tris-HCl buffer + 40 μL of substrate solution;

[0097] Sample group: 60 μL of sample solution to be tested + 140 μL of collagenase solution + 40 μL of substrate solution;

[0098] Control group: 60 μL Tris-HCl buffer + 140 μL collagenase solution + 40 μL substrate solution;

[0099] Blank group: 200 μL Tris-HCl buffer + 40 μL substrate solution. After placing each group in a 37℃ water bath for 20 min, the absorbance change of each component at 330 nm was measured. Each group was measured three times on average, and the average value was taken. The inhibition rate of collagenase by the sample groups was calculated using the following formula:

[0100] .

[0101] The results are shown in Table 2. Collagenase is an enzyme that can break down collagen. Inhibiting its activity can reduce collagen degradation, and by inhibiting collagen breakdown, it helps maintain skin elasticity and firmness. Therefore, the higher the collagenase clearance rate, the better the anti-aging performance of the composition.

[0102] Table 2. Results of efficacy tests on the composition

[0103] Group / Performance Hydroxyl radical scavenging rate / % Collagenase clearance rate / % Example 1 96.19 98.32 Example 2 94.20 97.29 Example 3 95.66 98.40 Example 4 91.43 93.50 Example 5 92.16 95.98 Example 6 93.01 96.53 Example 7 95.42 97.60 Example 8 88.15 93.77 Example 9 86.23 91.94 Example 10 85.79 90.52 Comparative Example 1 67.51 75.84 Comparative Example 2 75.46 77.46 Comparative Example 3 73.07 83.65 Comparative Example 4 78.43 80.28 Comparative Example 5 79.31 85.20 Comparative Example 6 83.67 86.93 Comparative Example 7 68.20 76.58 Comparative Example 8 71.95 82.36 Comparative Example 9 72.56 81.43 Comparative Example 10 70.82 79.90 Positive control group / 98.46

[0104] As shown in Table 2, combining the data from Examples 1-7 and Comparative Example 6, the whitening, brightening, and anti-aging properties of the composition in Comparative Example 6 were significantly reduced. This may be because the proportion of Artemia extract in Comparative Example 6 was too low, which affected the synergistic effect of each component. It can be seen that when the mass ratio of Artemia extract, Magnolia lily extract, Rosa desertica leaf cell extract, Lilium lancifolium orchid extract, and Lilium lancifolium extract in the composition is in the range of (0.2-2):(0.001-0.1):(0.001-0.1):(0.001-0.1):(0.001-0.1), the composition has a better whitening, brightening, and anti-aging effect. The performance of Examples 4-5 was also slightly lower than that of Examples 1-3, indicating that when the mass ratio of the Artemia extract, Magnolia lily extract, Rosa desertica leaf cell extract, Lilium lancifolium orchid extract and Lilium leucantha cell culture was (0.6-1.5):(0.02-0.05):(0.05-0.07):(0.03-0.08):(0.005-0.02), the brightening and anti-aging effects of the composition were at a better level.

[0105] Based on the data from Examples 1 and 8-10 in Table 2, it can be seen that the whitening, brightening, and anti-aging effects of the compositions in Examples 8-10 are lower than those in Example 1. This indicates that, under the premise of the same total number of live bacteria, fermentation with Bacillus coagulans, Lactobacillus johnsonii, and Saccharomyces rouxii in a live bacteria ratio of 1:(0.1-0.5):(1.5-3) can produce compositions with better whitening and anti-aging effects.

[0106] Based on the data from Example 1 and Comparative Examples 1-5 in Table 2, it can be seen that when one of the following is missing from the composition: Artemia extract, Magnolia liliiflora extract, Rosa desertica leaf cell extract, Lilium lancifolium extract, and Lilium lancifolium extract, the whitening, brightening, and anti-aging effects of the composition are significantly reduced. This indicates that the above five components synergistically enhance the whitening, brightening, and anti-aging effects of the composition.

[0107] Based on the data from Example 1 and Comparative Examples 7-10 in Table 2, it can be seen that the whitening, brightening, and anti-aging effects of the compositions in Comparative Examples 7-10 were significantly reduced. Specifically, the Artemia extract in Comparative Example 7 did not undergo a fermentation process during preparation, while Comparative Examples 8-10 lacked one of Bacillus coagulans, Lactobacillus johnsonii, or Saccharomyces rouxii, respectively. This indicates that Bacillus coagulans, Lactobacillus johnsonii, and Saccharomyces rouxii synergistically fermented Artemia, maximizing the yield, stability, and whitening and anti-aging effects of the components.

[0108] Application Examples 1, 8-10; Comparative Application Examples 1, 7-10

[0109] The anti-aging, brightening, and pore-minimizing compositions of Examples 1, 8-10 and Comparative Examples 1, 7-10 were added to the serum at a concentration of 1 wt% to obtain the serums of Application Examples 1, 8-10 and Comparative Application Examples 1, 7-10. The formulations are shown in Table 3.

[0110] The preparation methods of the serums in Application Examples 1, 8-10, and Comparative Application Examples 1, 7-10 include the following steps:

[0111] S1. Mix the humectant, thickener and 1 / 2 volume of deionized water, homogenize at 80°C, then add the preservative and stir until homogeneous to obtain the mixture.

[0112] S2. When the temperature of the mixture in S1 drops to 40°C, add the components of the anti-aging, brightening, and pore-shrinking composition, skin conditioning agent, remaining deionized water, and pH adjuster, and stir until homogeneous to obtain the essence.

[0113] Table 3. Serum formulations for Application Examples 1, 8-10 and Comparative Application Examples 1, 7-10

[0114]

[0115] Comparative Application Example 11

[0116] Compared to Application Example 11, the serum did not contain the anti-aging, brightening, and pore-minimizing composition. Instead, an equal amount of deionized water was used, and the preparation method was the same as in Application Example 1.

[0117] It should be noted that the serial numbers of the above comparative application examples are for matching with the examples and comparative examples; only 10 groups of serums were actually tested.

[0118] Test Example 3: Pore-minimizing efficacy test of serum on the human body

[0119] Sixty Asian adults aged 18-40 with enlarged pores (average pore diameter ≥ 0.1 mm, determined through pre-testing) and no serious skin diseases were randomly divided into 10 groups of 6 participants each. Volunteers applied samples (approximately 1 mL each of application examples 1, 8-10, and control examples 1, 7-11) to their entire face twice daily, morning and evening. Data was collected on day 0 and day 28. After each visit, volunteers washed their faces with facial cleanser and sat quietly for 30 minutes in an air-conditioned room at 21±1℃ and 50±10% humidity. The diameter of pores on the outer side of the nasolabial folds was measured using a Digimi C800 skin image analyzer. The evaluation parameter was calculated using the following formula: Skin pore diameter improvement rate (%) = (Mean skin pore diameter before use - Mean skin pore diameter after use) / Mean skin pore diameter before use × 100%. A higher skin pore diameter improvement rate indicates a better pore-shrinking effect of the serum. The data is shown in Table 4.

[0120] Table 4. Pore-minimizing efficacy test of serums on human body

[0121] Group / Performance Improvement rate of skin pore diameter (%) Application Example 1 46.5 Application Example 8 43.4 Application Example 9 41.3 Application Example 10 39.7 Comparative Application Example 1 23.3 Comparative Application Example 7 29.6 Comparative Application Example 8 36.2 Comparative Application Example 9 37.4 Comparative Application Example 10 35.7 Comparative application example 11 (blank control group) 7.8

[0122] Table 4 shows that, combining the data from Application Example 1 and Comparative Application Examples 7-10, the pore-shrinking ability of the serum in Comparative Application Example 7 is significantly lower than that of Application Example 1 and Comparative Application Examples 8-10. This may be because the Artemia extract in the serum of Comparative Application Example 7 is not fermented, lacking the newly added polysaccharides and other metabolites from fermentation, thus failing to address the issue of impaired activity of the active ingredients, and therefore unable to effectively enhance dermal support, barrier repair, and pore-shrinking effects. Compared to Application Example 1, the mixed bacterial solutions in Comparative Application Examples 8-10 lack one of Bacillus coagulans, Lactobacillus johnsonii, or Saccharomyces rouxii, respectively. The absence of one strain may disrupt the synergistic effect between microorganisms, affecting the fermentation effect. This indicates that the Artemia extract obtained by the synergistic fermentation of Bacillus coagulans, Lactobacillus johnsonii, and Saccharomyces rouxii can achieve the dual effects of dermal support and barrier repair.

[0123] Based on the data from Application Examples 1 and 8-10, it can be seen that when the total number of viable bacteria in the Artemia extract is the same, using Bacillus coagulans, Lactobacillus johnsonii, and Saccharomyces rouxii in a viable bacteria ratio of 1:(0.1-0.5):(1.5-3) can make the essence more effective in shrinking pores.

[0124] In summary, the composition of the present invention has good anti-aging, brightening and pore-shrinking effects.

[0125] Finally, it should be noted that the above embodiments are only used to illustrate the technical solutions of the present invention and are not intended to limit the scope of protection of the present invention. Although the present invention has been described in detail with reference to preferred embodiments, those skilled in the art should understand that modifications or equivalent substitutions can be made to the technical solutions of the present invention without departing from the essence and scope of the technical solutions of the present invention.

Claims

1. A composition for anti-aging, brightening, and pore-minimizing, characterized in that, The product comprises the following components: Artemia extract, Magnolia liliiflora extract, Rosa desertica leaf cell extract, Lilium lancifolium orchid extract, and Lilium lancifolium extract, wherein the mass ratio of Artemia extract, Magnolia liliiflora extract, Rosa desertica leaf cell extract, Lilium lancifolium orchid extract, and Lilium lancifolium extract is (0.6-1.5):(0.02-0.05):(0.05-0.07):(0.03-0.08):(0.005-0.02). The preparation method of the brine shrimp extract includes the following steps: S1. Select fresh brine shrimp that have been washed and drained, add 3-4 times the weight of deionized water and pulp them to obtain brine shrimp slurry. Add alkaline protease to the brine shrimp slurry for enzymatic hydrolysis, inactivate the enzyme and sterilize to obtain the hydrolysate. S2. Inoculate the enzymatic hydrolysate with a mixed bacterial culture of Bacillus coagulans, Lactobacillus johnsonii, and Saccharomyces rouxii, and ferment at 36-38℃ for 48-60 h to obtain the fermentation product; wherein, the inoculation amount of the mixed bacterial culture is 5-10% v / v of the enzymatic hydrolysate, the total viable count of the mixed bacterial culture is 1.5×10⁸-2×10⁹ CFU / mL, and the viable count ratio of Bacillus coagulans, Lactobacillus johnsonii, and Saccharomyces rouxii in the mixed bacterial culture is 1:(0.1-0.5):(1.5-3); the Bacillus coagulans was purchased from the China General Microbiological Culture Collection Center (CGMCC) with accession number CGMCC 1.10823; Lactobacillus johnsonii was purchased from the China General Microbiological Culture Collection Center (CGMCC) with accession number CGMCC 1.3260; and Saccharomyces rouxii was purchased from the China General Microbiological Culture Collection Center (CGMCC) with accession number CGMCC 2.2926; S3. The fermentation product is filtered to remove bacteria, and the filtrate is freeze-dried under vacuum to obtain the Artemia extract.

2. The anti-aging, brightening, and pore-minimizing composition as described in claim 1, characterized in that, In step S1 of the preparation of the Artemia extract, the amount of alkaline protease added is 2000-3000 U / g Artemia mass, the enzymatic hydrolysis temperature is 40-50℃, the pH is 8.5-9.0, and the time is 40-60 min.

3. The use of the anti-aging, brightening, and pore-minimizing composition according to any one of claims 1-2 in the preparation of cosmetics, characterized in that, The cosmetic is a toner, cream, mask, serum, or spray, and the amount of the composition added is 0.5%-3% of the total mass of the cosmetic.

4. An essence, characterized in that, The raw materials include the following percentages by weight: 0.5%-3% of the anti-aging, brightening, and pore-shrinking composition according to any one of claims 1-2, 0.1%-1% of a moisturizer, 0.3%-3.6% of a skin conditioning agent, 0.05%-0.5% of a thickener, 0.05%-0.1% of a preservative, and 0.01%-0.3% of a pH adjuster, with the balance being deionized water.

5. The essence as described in claim 4, characterized in that, The raw material is selected from at least one of (a)-(e): (a) The moisturizer is at least one of allantoin, betaine, β-glucan, trehalose, glycerin and sodium hyaluronate; (b) The skin conditioning agent is at least one of dipeptide-15, ribose, nicotinamide adenine dinucleotide and trisodium fructose diphosphate; (c) The thickener is xanthan gum and / or carbomer; (d) The preservative is 1,2-hexanediol and / or p-hydroxyacetophenone; (e) The pH adjuster is arginine and / or tromethamine.

6. The essence as described in claim 4, characterized in that, The skin conditioning agent comprises the following ingredients in weight percentages: dipeptide-15 0.1%-1.5%, ribose 0.2%-2%, nicotinamide adenine dinucleotide 0.003%-0.03%, and trisodium fructose diphosphate 0.001-0.01%.

7. The method for preparing the essence according to claim 4, characterized in that, Includes the following steps: (1) Mix the humectant, thickener and part of deionized water, homogenize at 75-85℃, add the preservative, stir evenly to obtain a mixture; (2) When the temperature of the mixture in S1 drops to 35-45℃, add the components of the anti-aging, brightening and shrinking pore composition, skin conditioning agent, remaining deionized water and pH adjuster, stir evenly to obtain the essence.

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