Pyridinium fluorescent probe compound, preparation method thereof and application of pyridinium fluorescent probe compound in cell mitochondrial staining
By synthesizing the pyridine salt fluorescent probe compound Y1OPY, the problem of labeling mitochondria in live cells has been solved, achieving low-toxicity and high-efficiency fluorescence imaging, which is suitable for mitochondrial research in live cells.
CN121698798APending Publication Date: 2026-03-20SHAOXING RES INST OF ZHEJIANG UNIV
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Patent Information
- Application Number
- CN202511701384.4
- Authority / Receiving Office
- CN · China
- Patent Type
- Applications(China)
- Current Assignee / Owner
- Filing Date
- 2025-11-19
- Publication Date
- 2026-03-20
AI Technical Summary
Technical Problem
Existing technologies struggle to efficiently and specifically label mitochondria without damaging living cells, and existing fluorescent probes lose their targeting ability in fixed cells.
Method used
A pyridine salt fluorescent probe compound, Y1OPY, was designed and synthesized via a specific synthetic pathway. It was then used to target and label mitochondrial membrane potential in living cells and imaged using a laser confocal microscope.
Benefits of technology
It enables rapid and specific labeling of mitochondria in live cells under low cytotoxic conditions, with clear laser confocal imaging, suitable for long-term live cell studies, reducing light damage and improving imaging resolution and signal-to-noise ratio.
✦ Generated by Eureka AI based on patent content.
Abstract
The invention discloses a pyridinium fluorescent probe compound as well as a preparation method and application thereof in cell mitochondrial staining, and belongs to the technical field of biomedical detection. The compound is formed by combining a 5-cyano indanone derivative and pyridinium salt, and the synthesis method is simple. The compound Y1OPy has large Stokes shift and low cytotoxicity, can specifically target living cell mitochondria, presents clear mitochondrial network structure fluorescence imaging, and does not have specific targeting to fixed cells, and the probe is suitable for real-time, dynamic and high-resolution fluorescence imaging of living cell mitochondria, and has wide application prospects. The method has a wide application prospect in the field of biomedical research.
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