Animal airway response induction real-time detection system and detection method
By expressing light-sensitive proteins in neurons of specific brain regions using optogenetics and then stimulating them with light, combined with signal acquisition and processing units, real-time and accurate detection of airway responses in animals was achieved. This solved the complexity and uncertainty problems of traditional methods, improved the accuracy and reliability of detection, and provided a new experimental model for respiratory disease research.
Patent Information
- Authority / Receiving Office
- CN · China
- Patent Type
- Applications(China)
- Current Assignee / Owner
- Filing Date
- 2024-09-26
- Publication Date
- 2026-03-27
AI Technical Summary
Traditional methods for detecting airway hyperresponsiveness are complex to operate, have poor real-time performance, uncontrollable parameters, and may cause adverse reactions. They also make it difficult to accurately control the activation level and spatiotemporal distribution of the vagus nerve.
Using optogenetics, the expression of photosensitive proteins in neurons of specific brain regions and light stimulation, combined with signal acquisition and processing units, can achieve precise activation of the vagus nerve and real-time detection of airway response. The optogenetic stimulation unit provides light stimulation to the experimental subjects, the signal acquisition unit receives airway hyperresponsive signals in real time, and the processing unit performs fitting calculations to obtain lung function indicators.
This technology enables real-time and accurate detection of airway responses in animals, improving the accuracy and reliability of detection, reducing trauma and stress to experimental subjects, ensuring the reproducibility of experimental results, and providing new experimental models and detection methods.
Smart Images
Figure CN121731676A_ABST
Abstract
Description
Technical Field
[0001] This application relates to the field of life science technology, and in particular to a real-time detection system and method for inducing animal airway responses. Background Technology
[0002] Airway hyperresponsiveness (AHR) is a key characteristic of respiratory diseases such as asthma, manifested as an overreaction of the airways to various stimuli. Traditional detection methods often rely on drug provocation or physical stimulation, which are complex to operate and lack real-time performance. Airway hyperresponsiveness is an important pathological feature of respiratory diseases such as asthma and chronic obstructive pulmonary disease, characterized by excessive sensitivity and premature airway contraction to multiple stimuli. The vagus nerve, as an important component of the autonomic nervous system, plays a significant regulatory role in the contraction and relaxation of airway smooth muscle. However, traditional methods struggle to precisely control the activation level and spatiotemporal distribution of the vagus nerve, limiting its application in airway hyperresponsiveness research. Optogenetics, as an emerging biological regulatory technique, introduces photosensitive proteins (such as photopigments or photosensitive ion channels) into specific cell types through genetic engineering and uses external light sources to precisely control the electrophysiological activities of these cells. This technology offers unprecedented possibilities for the precise regulation of neural activity in living animals.
[0003] The most similar approach: Current research has attempted bronchial provocation testing: This involves administering specific provocateurs (such as cold air, pollen, histamine, or methacholine) to induce bronchoconstriction and observing the response. Provocateurs are typically administered via nebulized inhalation or intravenous injection, followed by monitoring changes in respiratory function to assess airway hypersensitivity to various irritants. Bronchodilator testing: This aims to assess airway response to β-receptor agonists or anticholinergic drugs to determine the presence of reversible airway obstruction. Patients first undergo baseline pulmonary function testing, followed by oral or inhaled bronchodilator medication and a retest. The data before and after testing are compared to determine if the airway can be effectively dilated. Pulmonary function testing: This includes a series of respiratory movement tests designed to assess lung capacity, flow rate, and gas exchange efficiency, helping to identify potential airway hyperresponsiveness. Clinically, patients may need to complete a series of instructions under the guidance of a physician, such as inhaling deeply to maximum vital capacity and then exhaling forcefully.
[0004] Previous studies using specific stimulants to induce airway hyperresponsiveness in the bronchi have certain risks, potentially causing adverse reactions such as throat discomfort, bronchospasm, allergies (e.g., skin erythema, rash, urticaria), palpitations, difficulty breathing, and even respiratory failure. Furthermore, the procedures are complex, requiring professional personnel and specialized equipment, including the use of specific stimulants and devices, demanding a high level of expertise from the operators. During the testing process, additional irritants may need to be inhaled, potentially leading to varying degrees of discomfort. Moreover, the induction of airway hyperresponsiveness using these methods is not real-time, parameters are uncontrollable, and the results are inconsistent. Summary of the Invention
[0005] Therefore, it is necessary to provide a real-time detection system and method for inducing airway hyperresponsiveness in animals, which has advantages such as real-time performance, high accuracy, good repeatability, and minimal damage to animals, to address the shortcomings of current technologies that are not real-time, have uncontrollable parameters, and unstable effects.
[0006] To solve the above problems, this application adopts the following technical solution:
[0007] One of the objectives of this application is to provide a real-time detection system for inducing airway responses in animals, comprising:
[0008] The optogenetic stimulation unit is used to irradiate the photosensitive proteins of the experimental subject with a light source of a preset wavelength to stimulate neurons in a specific brain region of the experimental subject.
[0009] The signal acquisition unit is used to receive the airway hyperresponsiveness signal generated by the experimental subject after receiving light stimulation in real time, and to collect the lung function data of the experimental subject.
[0010] The processing unit is used to fit and calculate the collected data to obtain indicators of lung function and its capacity, thereby enabling real-time detection of airway hyperresponsiveness in animals.
[0011] In some embodiments, a light-sensitive protein gene is introduced into neurons in a specific brain region of the experimental subject, causing them to express the light-sensitive protein.
[0012] In some embodiments, a light-sensitive protein gene is introduced into neurons in a specific brain region of the experimental subject, causing them to express the light-sensitive protein, specifically:
[0013] The marker is delivered to the lungs of the experimental subject. After being taken up by the vagal nerve endings in the lungs, the marker is transported retrogradely along the nerve axon to the vagal nerve nuclei in the brainstem, so as to mark the vagal neurons projecting to the lungs.
[0014] The labeled vagal neurons are activated using optogenetics or other neuronal activation methods. By controlling the expression of the light-sensitive protein in neurons of specific brain regions, a recombinant plasmid containing the light-sensitive protein gene is constructed and transduced into the vagal ganglion or related neurons distributed along the vagus nerve of the experimental subject using a viral vector.
[0015] In some embodiments, the marker includes fluorescent gold or horseradish peroxidase, the photosensitive protein gene includes ChR2, and the viral vector includes AAV.
[0016] In some embodiments, the optogenetic stimulation unit can modulate a light source of a preset wavelength according to the modulation signal generated by the preset waveform, frequency, amplitude and duty cycle of the vagus neuron, and use the light source of the preset wavelength to irradiate the photosensitive protein of the experimental subject.
[0017] In some embodiments, the signal acquisition unit receives the rapid airway compression and closure phenomenon generated by the experimental subject after receiving light stimulation in real time, converts the acquired lung function data of the experimental subject into an electrical signal, and then obtains airway hyperresponsiveness based on the voltage value of the electrical signal.
[0018] In some embodiments, the processing unit receives the airway hyperresponsiveness generated by the experimental subject after receiving the light stimulation in real time and calculates indicators to measure lung function by body volume plethysmography, including lung volume, flow rate, and gas exchange efficiency.
[0019] In some embodiments, the processing unit includes an airway pressure tester implanted in the airway of the experimental subject. The stress interpolation generated by the airway contraction and relaxation is acquired by the processing unit, which then converts the acquired electrical signal into a digital signal for the experimenter to read, thereby enabling real-time detection of airway hyperresponsiveness in animals.
[0020] In some embodiments, a camera unit is also included for observing and recording the experimental subject, including rodents.
[0021] A second objective of this application is to provide a detection method for the aforementioned real-time detection system for inducing animal airway responses, comprising the following steps:
[0022] The light-sensitive protein of the experimental subject is illuminated by a light source of a preset wavelength, thereby stimulating neurons in a specific brain region of the experimental subject with light.
[0023] The system receives the airway hyperresponsiveness signal generated by the experimental subject after receiving light stimulation in real time, and collects the lung function data of the experimental subject.
[0024] The collected data are fitted and calculated to obtain indicators of lung function and its capacity, enabling real-time detection of airway hyperresponsiveness in animals.
[0025] The present application adopts the above technical solution, and its beneficial effects are as follows:
[0026] The real-time detection system and method for induced airway response in animals provided in this application utilizes an optogenetic stimulation unit to irradiate photosensitive proteins in experimental subjects with a light source of a preset wavelength, thereby stimulating neurons in specific brain regions of the subjects. A signal acquisition unit receives the airway hyperresponsiveness signals generated by the subjects after receiving light stimulation in real time and collects lung function data. A processing unit performs fitting calculations on the collected data to derive indicators of lung function and its capacity, achieving real-time detection of airway hyperresponsiveness in animals. This application employs optogenetic technology, allowing for precise temporal and spatial control of vagal nerve activation, enabling real-time detection of airway responses in animals and improving the accuracy and reliability of experimental results. Standardized experimental procedures and light source parameter settings ensure the reproducibility of experimental results. The minimally invasive photostimulation method reduces trauma and stress responses to experimental subjects. Gene editing technology controls the expression of photosensitive proteins, avoiding the complexity and uncertainty of traditional methods, providing a new experimental model and detection method for the study of respiratory diseases such as asthma. The above detection system and method are not only applicable to the study of airway hyperresponsiveness diseases but can also be extended to the study of other diseases related to vagal nerve activity. Attached Figure Description
[0027] To more clearly illustrate the technical solutions of the embodiments of this application, the drawings used in the description of the embodiments of this application or the prior art will be briefly introduced below. Obviously, the drawings described below are only some embodiments of this application. For those skilled in the art, other drawings can be obtained based on these drawings without creative effort.
[0028] Figure 1 This is a schematic diagram of the animal airway response induction real-time detection system provided in Embodiment 1 of the present invention.
[0029] Figure 2 This is a schematic diagram of non-invasive real-time detection of airway hyperresponsiveness before and after light stimulation, as provided in Embodiment 1 of the present invention.
[0030] Figure 3 This is a schematic diagram of real-time detection of invasive airway hyperresponsiveness before and after light stimulation, as provided in Embodiment 1 of the present invention.
[0031] Figure 4The flowchart shows the steps of the detection method of the real-time detection system for inducing animal airway response provided in Embodiment 2 of the present invention. Detailed Implementation
[0032] The embodiments of this application are described in detail below. Examples of these embodiments are shown in the accompanying drawings, wherein the same or similar reference numerals denote the same or similar elements or elements having the same or similar functions throughout. The embodiments described below with reference to the accompanying drawings are exemplary and intended to explain this application, and should not be construed as limiting this application.
[0033] In the description of this application, it should be understood that the terms "upper", "lower", "horizontal", "inner", "outer", etc., indicate the orientation or positional relationship based on the orientation or positional relationship shown in the accompanying drawings, and are only for the convenience of describing this application and simplifying the description, and do not indicate or imply that the device or element referred to must have a specific orientation, or be constructed and operated in a specific orientation, and therefore should not be construed as a limitation of this application.
[0034] Furthermore, the terms "first" and "second" are used for descriptive purposes only and should not be construed as indicating or implying relative importance or implicitly specifying the number of technical features indicated. Thus, a feature defined as "first" or "second" may explicitly or implicitly include one or more of that feature. In the description of this application, "multiple" means two or more, unless otherwise explicitly specified.
[0035] To make the objectives, technical solutions, and advantages of this application clearer, the following detailed description is provided in conjunction with the accompanying drawings and embodiments.
[0036] Example 1
[0037] Please see Figure 1 This is a schematic diagram of a real-time detection system for inducing animal airway responses, provided in an embodiment of this application. It includes an optogenetic stimulation unit 110, a signal acquisition unit 120, and a processing unit 130. The function of each component is described in detail below.
[0038] The optogenetic stimulation unit 110 is used to irradiate the photosensitive protein of the experimental subject with a light source of a preset wavelength to stimulate neurons in a specific brain region of the experimental subject.
[0039] In this embodiment, a photosensitive protein gene is introduced into neurons in a specific brain region of the experimental subject, causing them to express the photosensitive protein.
[0040] Specifically, the marker is delivered to the lungs of the experimental subject. After being taken up by the vagal nerve endings in the lungs, the marker is transported retrogradely along the nerve axons to the vagal nerve nuclei in the brainstem, thereby marking the vagal neurons projecting to the lungs. The marked vagal neurons are then activated using optogenetics or other neuronal activation methods. By controlling the expression of the light-sensitive protein in neurons of specific brain regions, a recombinant plasmid containing the light-sensitive protein gene is constructed and transduced into the vagal ganglion or related neurons distributed along the vagus nerve of the experimental subject using a viral vector.
[0041] In this embodiment, retrograde vagal labeling is performed using retrograde markers with high affinity and low toxicity, such as fluorescent gold or horseradish peroxidase. The markers are precisely delivered to the lungs of the experimental subjects via intratracheal injection, intravenous injection, or a combination of localized injection. After being taken up by the vagal nerve endings in the lungs, the markers are transported retrogradely along the nerve axons to the vagal nuclei in the brainstem, achieving precise labeling of the vagal neurons projecting to the lungs.
[0042] In this embodiment, a suitable photosensitive protein gene is selected, such as ChR2 (a photosensitive ion channel from bacteria), which opens cation channels under blue light irradiation, causing cell depolarization. The ChR2 gene is inserted into a viral vector (such as AAV) to construct a recombinant virus. AAV vectors are widely used in optogenetic research due to their low immunogenicity, high tissue specificity, and non-pathogenicity. The recombinant virus is amplified in an appropriate cell line and purified to obtain a high-titer viral suspension. The viral suspension is precisely introduced into the vagus ganglion or neurons distributed along the vagus nerve of the experimental subjects via stereotactic injection or nasal instillation.
[0043] It should be noted that in practice, different light-sensitive protein genes can be used to further improve the accuracy and specificity of measurements and adapt to different experimental needs and application scenarios.
[0044] It is understood that this embodiment controls the expression of photosensitive proteins through gene editing technology, thus avoiding the complexity and uncertainty of traditional methods.
[0045] It should be noted that, due to the characteristics of vagus neurons, the optogenetic stimulation unit 100 can modulate a light source of a preset wavelength according to the modulation signal generated by the preset waveform, frequency, amplitude and duty cycle of the vagus neurons, and use the light source of the preset wavelength to irradiate the photosensitive protein of the experimental subject.
[0046] In this embodiment, the photostimulation unit 110 includes a light source of appropriate wavelength (such as a 473nm blue laser) and an optical fiber coupling device to ensure that the light source can accurately irradiate the vagus nerve region of the experimental subject. Appropriate parameters such as light intensity, duration, and frequency are set according to the photosensitivity characteristics of ChR2 and experimental requirements. The experimental subject is placed in a specially designed experimental device, and the light source is guided to the vagus nerve region for photostimulation via optical fiber.
[0047] It is understood that this embodiment ensures the repeatability of experimental results through standardized experimental procedures and light source parameter settings; and the localized, minimally invasive light stimulation method reduces trauma and stress response to the experimental subjects.
[0048] In this embodiment, the experimental subject is placed in a specific light stimulation unit 110, and the vagus nerve region is irradiated with a light source of specific wavelength and intensity to activate neurons expressing photosensitive proteins. By adjusting the light source parameters (such as light intensity, duration, frequency, etc.), precise control of vagus nerve activity can be achieved. The photosensitive proteins are irradiated with light of a preset wavelength to photostimulate neurons in the specific brain region. Simultaneously, the physiological responses and behavioral changes of the experimental subject are recorded.
[0049] It is understood that this embodiment uses optogenetic technology to allow precise control of vagal nerve activation in time and space, enabling real-time measurement of airway responses in animals, thus improving the accuracy and reliability of the measurement and experimental results.
[0050] The signal acquisition unit 120 is used to receive the airway hyperresponsive signal generated by the experimental subject after receiving light stimulation in real time, and to collect the lung function data of the experimental subject.
[0051] Specifically, the signal acquisition unit 120 receives the rapid airway compression and closure phenomenon generated by the experimental subject after receiving light stimulation in real time, converts the lung function data of the experimental subject into an electrical signal, and then obtains the airway hyperresponsiveness based on the voltage value of the electrical signal.
[0052] Furthermore, the signal acquisition unit 120 can also record the correspondence between waveform, frequency, amplitude, duty cycle generation and the response amount of airway hyperresponsiveness at a preset wavelength.
[0053] It is understood that the signal acquisition unit 120 can also acquire and process data from the respiratory measurement device and the optogenetic unit 110. The signal acquisition unit 120 may include a data logger, signal processor, or data analysis software to monitor and analyze the respiration and nerve cell activity of experimental animals in real time. In practice, the design of the data acquisition device should consider the speed and accuracy of data processing. It should be able to record and process large amounts of data in real time and output the processing results promptly to control the feedback function of the system.
[0054] The processing unit 130 is used to fit and calculate the collected data to obtain indicators of lung function and its capacity, thereby realizing real-time detection of airway hyperresponsiveness in animals.
[0055] Please see Figure 2 This is a schematic diagram of non-invasive real-time detection of airway hyperresponsiveness before and after light stimulation, provided in this embodiment.
[0056] In this embodiment, the processing unit 130 receives the airway hyperresponsiveness generated by the experimental subject after receiving the light stimulation in real time and calculates indicators for measuring lung function by body volume plethysmography. The indicators include lung volume, flow rate and gas exchange efficiency.
[0057] Furthermore, a pulmonary function testing instrument 131 (such as the Penh system) is used to monitor changes in airway resistance in mice before and after light stimulation, and airway responsiveness is assessed by measuring parameters such as respiratory rate and tidal volume; the signal acquisition unit 120 includes a data logger 121, a signal processor 122 or data analysis software, etc., to monitor and analyze the respiration and nerve cell activity of experimental animals in real time.
[0058] Please see Figure 3 This is a schematic diagram of real-time detection of invasive airway hyperresponsiveness before and after light stimulation, provided in this embodiment.
[0059] In this embodiment, the processing unit 130 includes an airway pressure tester 131, which is implanted into the airway of the experimental subject. The stress interpolation generated by the airway contraction and relaxation is acquired by the processing unit 130, which then converts the acquired electrical signal into a digital signal for the experimental personnel to read, thereby achieving real-time detection of airway hyperresponsiveness in animals. The signal acquisition unit 120 includes a data logger 121, a signal processor 122, or data analysis software, etc., to monitor and analyze the respiration and nerve cell activity of experimental animals in real time.
[0060] It is understandable that invasive procedures, such as endotracheal intubation and mechanical ventilation, are performed when necessary to more accurately measure parameters such as airway pressure and flow.
[0061] Furthermore, the real-time detection system for inducing animal airway responses provided in the above embodiments of this application also includes a camera unit (not shown in the figure). The camera unit is used to observe and record the experimental subject to better understand the overall movement and posture of the animal's response. The experimental subject includes rodents, such as mice.
[0062] The real-time detection system for induced airway responses in animals provided in Embodiment 1 of this application utilizes optogenetic technology to allow precise temporal and spatial control of vagal nerve activation, enabling real-time detection of animal airway responses and improving the accuracy and reliability of experimental results. Standardized experimental procedures and light source parameter settings ensure the reproducibility of experimental results. The minimally invasive photostimulation method reduces trauma and stress on experimental subjects. Gene editing technology controls the expression of photosensitive proteins, avoiding the complexity and uncertainty of traditional methods, providing a new experimental model and detection method for the study of respiratory diseases such as asthma. The aforementioned detection system and method are not only applicable to the study of airway hyperresponsiveness diseases but can also be extended to the study of other diseases related to vagal nerve activity.
[0063] Example 2
[0064] Please see Figure 4 The flowchart below shows the steps of the detection method of the real-time detection system for inducing animal airway response provided in Embodiment 2, which includes the following steps:
[0065] S110: Irradiate the photosensitive protein of the experimental subject with a light source of a preset wavelength to stimulate neurons in a specific brain region of the experimental subject with light.
[0066] S120: Receives in real time the airway hyperresponsiveness signal generated by the experimental subject after receiving light stimulation, and collects the lung function data of the experimental subject;
[0067] S130: The collected data is fitted and calculated to obtain indicators of lung function and its capacity, enabling real-time detection of airway hyperresponsiveness in animals.
[0068] The detailed implementation of the real-time detection method for inducing animal airway response provided in this embodiment can be found in Embodiment 1, and will not be repeated here.
[0069] The real-time detection method for induced airway responses in animals provided in Embodiment 1 of this application utilizes optogenetic technology to allow precise temporal and spatial control of vagal nerve activation, enabling real-time detection of animal airway responses and improving the accuracy and reliability of experimental results. Standardized experimental procedures and light source parameter settings ensure the reproducibility of experimental results. The minimally invasive photostimulation method reduces trauma and stress on experimental subjects. Gene editing technology controls the expression of photosensitive proteins, avoiding the complexity and uncertainty of traditional methods, providing a new experimental model and detection method for the study of respiratory diseases such as asthma. The above detection system and method are not only applicable to the study of airway hyperresponsiveness diseases but can also be extended to the study of other diseases related to vagal nerve activity.
[0070] The above are merely preferred embodiments of this application, and only specifically describe the technical principles of this application. These descriptions are only for explaining the principles of this application and should not be construed as limiting the scope of protection of this application in any way. Based on this explanation, any modifications, equivalent substitutions, and improvements made within the spirit and principles of this application, as well as other specific embodiments of this application that can be conceived by those skilled in the art without creative effort, should be included within the scope of protection of this application.
Claims
1. A real-time detection system for inducing airway responses in animals, characterized in that, include: The optogenetic stimulation unit is used to irradiate the photosensitive proteins of the experimental subject with a light source of a preset wavelength to stimulate neurons in a specific brain region of the experimental subject. The signal acquisition unit is used to receive the airway hyperresponsiveness signal generated by the experimental subject after receiving light stimulation in real time, and to collect the lung function data of the experimental subject. The processing unit is used to fit and calculate the collected data to obtain indicators of lung function and its capacity, thereby enabling real-time detection of airway hyperresponsiveness in animals.
2. The real-time detection system for inducing animal airway responses as described in claim 1, characterized in that, The experiment subjects had a light-sensitive protein gene introduced into neurons in a specific brain region, causing them to express the light-sensitive protein.
3. The real-time detection system for inducing animal airway responses as described in claim 2, characterized in that, The experiment subjects had a light-sensitive protein gene introduced into neurons in a specific brain region, causing them to express the light-sensitive protein, specifically: The marker is delivered to the lungs of the experimental subject. After being taken up by the vagal nerve endings in the lungs, the marker is transported retrogradely along the nerve axon to the vagal nerve nuclei in the brainstem, so as to mark the vagal neurons projecting to the lungs. The labeled vagal neurons are activated using optogenetics or other neuronal activation methods. By controlling the expression of the light-sensitive protein in neurons of specific brain regions, a recombinant plasmid containing the light-sensitive protein gene is constructed and transduced into the vagal ganglion or related neurons distributed along the vagus nerve of the experimental subject using a viral vector.
4. The real-time detection system for inducing animal airway responses as described in claim 3, characterized in that, The markers include fluorescent gold or horseradish peroxidase, the photosensitive protein gene includes ChR2, and the viral vector includes AAV.
5. The real-time detection system for inducing animal airway responses as described in claim 3, characterized in that, The optogenetic stimulation unit can modulate a light source of a preset wavelength according to the modulation signal generated by the preset waveform, frequency, amplitude and duty cycle of the vagus neuron, and use the light source of the preset wavelength to irradiate the photosensitive protein of the experimental subject.
6. The real-time detection system for inducing animal airway responses as described in claim 5, characterized in that, The signal acquisition unit receives the rapid airway compression and closure phenomenon generated by the experimental subject after receiving light stimulation in real time, and converts the lung function data of the experimental subject into an electrical signal, and then obtains the airway hyperresponsiveness based on the voltage value of the electrical signal.
7. The real-time detection system for inducing animal airway responses as described in claim 6, characterized in that, The processing unit receives the airway hyperresponsiveness generated by the experimental subject after receiving the light stimulation in real time and calculates indicators to measure lung function by body volume plethysmography. The indicators include lung volume, flow rate, and gas exchange efficiency.
8. The real-time detection system for inducing animal airway responses as described in claim 6, characterized in that, The processing unit includes an airway pressure tester, which is implanted into the airway of the experimental subject. The stress interpolation generated by the airway contraction and relaxation is acquired by the processing unit, which then converts the acquired electrical signal into a digital signal for the experimental personnel to read, thereby achieving real-time detection of airway hyperresponsiveness in animals.
9. The real-time detection system for inducing animal airway responses as described in claim 1, characterized in that, It also includes a camera unit for observing and recording the experimental subjects, including rodents.
10. A detection method for the real-time detection system for inducing animal airway responses as described in claim 1, characterized in that, Includes the following steps: The light-sensitive protein of the experimental subject is illuminated by a light source of a preset wavelength, thereby stimulating neurons in a specific brain region of the experimental subject with light. The system receives the airway hyperresponsiveness signal generated by the experimental subject after receiving light stimulation in real time, and collects the lung function data of the experimental subject. The collected data are fitted and calculated to obtain indicators of lung function and its capacity, enabling real-time detection of airway hyperresponsiveness in animals.