Qi-benefiting, blood-nourishing and sleep-conditioning Yuanling paste as well as preparation method and application thereof

Abstract

The invention discloses Yulingling paste with functions of benefiting qi, nourishing blood and regulating sleep as well as a preparation method and application thereof, and belongs to the technical field of traditional Chinese medicine formulas. The Yuling ointment provided by the invention is composed of 13 medicinal and edible or beneficial Chinese herbal medicines or components, the materials have no toxic or side effect, and the Yuling ointment prepared by a specific preparation method after the raw materials are prepared according to a ratio is mild in nature, is not cold or cool or dry-heat, is deficiency of qi and blood, and is relatively weak in organism after being eaten by a certain amount of Yuling ointment. The effects of benefiting qi, nourishing blood, improving sleep quality, improving viscera health degree and absorbing and utilizing nutrient substances can be achieved within limited time, and the effects of consolidating basis and promoting the body to form a virtuous circle are fundamentally achieved.

Description

Technical Field

[0001] This invention relates to the field of traditional Chinese medicine formulation technology, and in particular to a Yuling ointment with functions of replenishing qi and blood and regulating sleep, as well as its preparation method and application. Background Technology

[0002] Qi and blood deficiency, also known as qi deficiency and blood deficiency in Traditional Chinese Medicine (TCM), is a common sub-health condition that can lead to decreased organ function, weakened immunity, and accelerated aging. Typical manifestations include: 1) Physical symptoms: aversion to cold, spontaneous sweating, dizziness and tinnitus, fatigue and weakness, palpitations and shortness of breath, reluctance to speak, cold and numb hands and feet, blurred vision, scanty menstruation, irregular menstrual cycles or even amenorrhea, pale tongue and weak pulse, developmental delay, difficulty falling asleep, easily startled and awakened, insomnia and dreaminess, frequent urination at night, susceptibility to colds, slow wound healing, and recurrent infections; 2) External observable characteristics: cloudy, yellowish, and bloodshot eyes, pale or sallow complexion, dry skin, slow hair growth, dry, brittle, and split ends, pale lips and nails, thin, brittle, and easily broken nails, and receding gums; 3) Mental state: mental confusion, poor concentration, forgetfulness, low mood or irritability.

[0003] Common causes of qi and blood deficiency include: 1) Insufficient congenital endowment, such as weak spleen and stomach, deficiency of liver and kidney, etc., which affect the generation of qi and blood; 2) Excessive consumption, such as long-term staying up late, high-intensity work, frequent childbirth or large blood loss, which leads to qi and blood overdraft; 3) Improper diet, such as dieting, unbalanced diet (such as excessive vegetarianism), overeating, which damages the spleen and stomach's digestive function and leads to insufficient qi and blood generation; 4) Chronic diseases, such as anemia, hypothyroidism, digestive system diseases, etc., which consume qi and blood in the long term. Summary of the Invention

[0004] The purpose of this invention is to provide a Yuling ointment with the functions of replenishing qi and blood and regulating sleep, as well as its preparation method and application, in order to solve the problems of traditional Yuling ointment having a single composition, weak efficacy, slow onset of action, time-consuming preparation method, and the loss of many active ingredients due to the high temperature in the traditional method, resulting in low resource utilization.

[0005] To achieve the above objectives, the present invention provides a Yuling Paste with the functions of replenishing qi and blood and regulating sleep, which is composed of the following components: American ginseng, longan pulp, black nightshade fruit, snow lotus culture, red ginseng, Kacip Fatimah, dried mulberry, prepared rehmannia root, magnesium L-threonate, γ-aminobutyric acid, Ophiopogon japonicus, Ganoderma lucidum and goose muscle peptide.

[0006] Preferably, it is composed of the following components in parts by weight: 2-3 parts American ginseng, 3-6 parts red ginseng, 7-12 parts Kacip Fatimah, 5-8 parts Rehmannia glutinosa, 2-5 parts Ganoderma lucidum, 25-30 parts snow lotus culture, 2-8 parts black nightshade fruit, 15-20 parts Ophiopogon japonicus, 10-20 parts dried mulberry, 20-30 parts longan pulp, 3-8 parts magnesium L-threonate, 3-8 parts γ-aminobutyric acid and 5-10 parts anserine peptide.

[0007] Preferably, it is composed of the following components in parts by weight: 2.5 parts American ginseng, 6 parts red ginseng, 10 parts Kacip Fatimah, 7 parts Rehmannia glutinosa, 3 parts Ganoderma lucidum, 28 parts snow lotus culture, 5 parts black nightshade fruit, 17 parts Ophiopogon japonicus, 20 parts dried mulberry, 25 parts longan pulp, 6 parts magnesium L-threonate, 5 parts γ-aminobutyric acid and 5 parts anserine peptide.

[0008] Preferably, it is composed of the following components in parts by weight: 2.5 parts American ginseng, 3 parts red ginseng, 10 parts Kacip Fatimah, 7 parts Rehmannia glutinosa, 3 parts Ganoderma lucidum, 28 parts snow lotus culture, 5 parts black nightshade fruit, 17 parts Ophiopogon japonicus, 20 parts dried mulberry, 25 parts longan pulp, 6 parts magnesium L-threonate, 5 parts γ-aminobutyric acid and 10 parts anserine peptide.

[0009] Preferably, it is composed of the following components in parts by weight: 2.5 parts American ginseng, 6 parts red ginseng, 10 parts Kacip Fatimah, 7 parts Rehmannia glutinosa, 3 parts Ganoderma lucidum, 28 parts snow lotus culture, 5 parts black nightshade fruit, 17 parts Ophiopogon japonicus, 10 parts dried mulberry, 25 parts longan pulp, 6 parts magnesium L-threonate, 5 parts γ-aminobutyric acid and 10 parts anserine peptide.

[0010] The preparation method of Yuling Paste, which has the functions of replenishing qi and blood and regulating sleep as described above, includes the following steps: S1. Remove dust and impurities from American ginseng, red ginseng, Kacip Fatima, and Ganoderma lucidum, then freeze-dry them and slice, cut into pieces, crush, grind into powder, and stir-fry at low temperature. Freeze-dry snow lotus culture and grind it into powder. Mix the low-temperature stir-fried combination with snow lotus culture powder and add drinking water for ultrasonic homogenization. S2. Remove dust and impurities from longan pulp, black nightshade fruit, ophiopogon japonicus, dried mulberry, and prepared rehmannia root, cut them into small pieces, and then add drinking water for ultrasonic homogenization. S3. After ultrasonic homogenization of S1 and S2, stir and mix all the components. Then add L-magnesium threonate, γ-aminobutyric acid and chemoresine, stir and mix again. Keep the mixture at 62~65℃ for 90~120min, and then bottle it aseptically.

[0011] Preferably, in S1, the powder is passed through an 80-mesh sieve after grinding; the low-temperature frying involves mixing the sieved powders of each component at 50-65°C for 1-2 hours; and the volume ratio of drinking water added during ultrasonic homogenization to the mixture is 1:1.

[0012] Preferably, the volume of the small pieces cut in S2 is no greater than 0.125 cm. 3 The volume ratio of drinking water added to the mixture was 1:1; the ultrasonic homogenization in S1 and S2 was performed at 100 Hz for 30-40 min.

[0013] Preferably, in S3, the temperature is maintained at 62-65°C for 90-120 minutes to make the water content of the mixture 60-75%.

[0014] The application of Yuling Paste, which has the functions of replenishing qi and blood and regulating sleep, as described above, in the preparation of food products that replenish qi and blood and regulate sleep.

[0015] American ginseng, a traditional Chinese medicine, is the root of the perennial herbaceous plant *Panax quinquefolius*, belonging to the Araliaceae family. It is harvested in autumn, and after removing branches and rootlets, it is sun-dried or oven-dried to obtain raw American ginseng. Raw American ginseng has a sweet and slightly pungent taste, and is cool in nature. It enters the heart, lung, and kidney meridians, and has the effects of nourishing lung yin, clearing deficiency fire, replenishing the qi and blood of the heart and spleen, and promoting body fluid production and quenching thirst. It is used to treat chronic cough due to lung deficiency, blood loss, overwork, dry throat and thirst, and fatigue due to deficiency heat.

[0016] Longan pulp, a traditional Chinese medicine, is the fruit of the longan plant (Sapindaceae family). Dimocarpus longan The aril of Lour. is sweet and warm in nature, and enters the heart and spleen meridians. It has the effects of nourishing the heart and spleen, nourishing blood and calming the mind. It is often used for qi and blood deficiency, palpitations, forgetfulness and insomnia, and blood deficiency and chlorosis.

[0017] Black nightshade fruit is the mature fruit of black nightshade, an annual herbaceous plant belonging to the Solanaceae family and the Solanum genus. It is dark purple and contains yellow, oval, sesame-like seeds. It has both medicinal and edible value, contains anthocyanins, and is cold in nature. Its main effects include clearing heat and detoxifying, reducing swelling and promoting diuresis, and relieving cough and asthma.

[0018] Snow lotus (Saussurea involucrate (Kar. et Kir.) Sch. Bip) is a precious traditional Chinese medicine unique to Xinjiang. Uyghur medicine believes that snow lotus tonifies the kidneys, invigorates blood circulation, strengthens muscles and bones, nourishes nerves, and regulates abnormal body fluids. Traditional Chinese medicine believes that snow lotus has the effects of warming the kidneys and strengthening yang, dispelling wind and dampness, and promoting blood circulation.

[0019] Red ginseng, also known as red ginseng, has a sweet and slightly bitter taste, a slightly cold nature, and a strong aroma. It has excellent effects in replenishing vital energy, strengthening yang and preventing collapse. It is often used for lung deficiency cough, spleen deficiency with chronic diarrhea and dysentery, stomach cold due to deficiency of the middle jiao, dizziness due to blood deficiency, and shortness of breath due to kidney deficiency.

[0020] Kacip Fatimah, botanically known as Labisia Pumila, is a common herb that grows in the tropical rainforests of Southeast Asia. According to available information, Kacip Fatimah is rich in phytoestrogens and saponins, which can regulate menstruation, alleviate menstrual cramps, and also have potential effects such as anti-oxidation, anti-inflammation, and endocrine regulation.

[0021] Mulberries are a sweet and sour fruit rich in anthocyanins, which have antioxidant, blood-nourishing, iron-deficiency anemia-preventing, vision-improving, bowel-regulating, spleen-strengthening, digestion-aiding, and thirst-quenching effects.

[0022] Rehmannia glutinosa, a traditional Chinese medicine, is the fresh or dried tuberous root of the plant Rehmannia glutinosa Libosch., belonging to the Scrophulariaceae family. It can be used fresh or processed. Depending on the processing method, processed Rehmannia glutinosa is divided into raw Rehmannia glutinosa (sun-dried) and prepared Rehmannia glutinosa (steamed). Prepared Rehmannia glutinosa has the effects of nourishing blood and replenishing essence, benefiting the liver and kidneys, with a focus on nourishing liver blood. It also improves heart function and nourishes the kidneys. It is used to improve pale complexion, dizziness, palpitations, and scanty menstruation caused by blood deficiency, and to regulate lower back and knee weakness, tinnitus, and premature graying of hair caused by liver and kidney deficiency.

[0023] Ophiopogon japonicus, the dried tuberous root of the Liliaceae plant Ophiopogon japonicus (also known as Liriope muscari), was included in the list of substances that are traditionally used as both food and Chinese medicine in 2024. It has a sweet and slightly bitter taste, is slightly cold in nature, and enters the heart, lung, and stomach meridians. It has the effects of nourishing yin and promoting body fluids, moistening the lungs and relieving cough, clearing the heart and relieving irritability, and moistening the intestines and promoting bowel movements. Animal experiments have shown that Ophiopogon japonicus extract can enhance immunity and delay cellular oxidative damage. It is used for symptoms such as thirst and dry cough with hemoptysis due to deficiency of lung and stomach yin, palpitations and easy fright due to deficiency of heart yin, and heat damage to body fluids in the later stages of febrile diseases.

[0024] Reishi mushroom is a traditional medicinal fungus. It is sweet in taste and neutral to slightly warm in nature. It enters the heart, lung, liver, and kidney meridians. It has the effects of nourishing heart blood, benefiting heart qi, and calming the mind. It can be used to treat symptoms such as restlessness, insomnia, palpitations, dreaminess, forgetfulness, fatigue, and poor appetite caused by insufficient qi and blood and malnourishment of the heart. It enters the lung meridian to nourish lung qi, warm the lungs, resolve phlegm, stop cough, and relieve asthma. It has the effect of nourishing qi and blood and is often used to treat symptoms such as weakness and shortness of breath, loss of appetite, cold hands and feet, or irritability and dry mouth.

[0025] Imidazole dipeptides are a class of compounds formed by the peptide bond linking histidine and β-alanine, containing an imidazole ring (a five-membered heterocycle with two nitrogen atoms). Typical examples of imidazole dipeptides include carnosine and anserine. Anserine (β-ca-alanine-1-methyl-L-histidine) is a naturally occurring water-soluble dipeptide found in the skeletal muscle and brain tissue of vertebrates. Its chemical structure features methylation modification, ensuring stability in low pH environments. Studies have shown that it has regulatory effects on uric acid metabolism (inhibiting xanthine oxidase activity to reduce uric acid synthesis, while promoting renal tubular uric acid excretion and lowering blood uric acid concentration), neurometabolism (regulating central histamine metabolism, lowering cortisol concentration, and alleviating exercise-induced fatigue), antioxidant effects, and cell protection.

[0026] L-Magnesium threonate is an organic magnesium compound with high bioavailability due to its unique structure. It not only replenishes the body's magnesium levels—a crucial function for maintaining normal physiological functions such as muscle contraction, nerve signal transmission, and electrolyte balance—but also crosses the blood-brain barrier to act on the central nervous system. Studies have shown that L-Magnesium threonate can enhance synaptic plasticity, reduce the risk of neuroinflammation, and has potential regulatory effects on mood problems such as anxiety and depression. It can also help relax nerves, effectively relieving insomnia and shallow sleep, shortening sleep onset time, and prolonging the deep sleep stage. Furthermore, research indicates that L-Magnesium threonate can indirectly reduce free radical damage, support glucose metabolism, and improve insulin sensitivity.

[0027] Gamma-aminobutyric acid (GABA) is an important and widely distributed inhibitory neurotransmitter in the mammalian brain. It can reduce neuronal excitability, maintain the dynamic balance of the nervous system, prevent excessive brain activity, prevent abnormal discharges (such as epileptic seizures), and help the brain remain stable under stress or stimulation. It also plays a role in stabilizing mood, improving sleep, and relieving anxiety.

[0028] Yu Ling Gao is a traditional nourishing food therapy recipe originating from the Qing Dynasty's "Sui Xi Ju Diet Manual". It is made with longan pulp and American ginseng as the main ingredients and is made through a steaming process (traditionally requiring more than 40 hours of steaming). Its core effects are to replenish qi and blood and nourish the heart and spleen. It is suitable for symptoms such as fatigue, insomnia, and pale complexion caused by insufficient qi and blood.

[0029] Therefore, the specific technical effects of the Jade Spirit Paste provided by this invention, which has the functions of replenishing qi and blood and regulating sleep, are as follows: (1) The Jade Spirit Paste provided by the present invention consists of 13 ingredients. First, it achieves the effect of nourishing blood and qi by using a specific ratio of American ginseng, longan pulp, red ginseng, mulberry, prepared rehmannia root, ophiopogon root, and ganoderma. Then, it regulates the body fluid and endocrine system by using black nightshade fruit, snow lotus culture, Kacip Fatimah, and goose muscle peptide, thereby achieving the effect of promoting the circulation of qi and blood. Furthermore, through American ginseng (enters the heart, lung, and kidney meridians, nourishing / tonifying the heart and spleen), longan pulp (tonifying the heart and spleen), snow lotus culture (warming the kidneys), red ginseng (tonifying the spleen and stomach, strengthening the kidneys and consolidating yang), mulberry (improving eyesight and strengthening the spleen), prepared rehmannia root (benefiting the liver and kidneys, improving heart function), ophiopogon root (enters the heart, lung, and stomach meridians, moistening the lungs, nourishing the stomach, and moistening the intestines), ganoderma lucidum (enters the heart, lung, liver, and kidneys, tonifying the heart, warming the lungs, and benefiting lung qi), and goose muscle peptide (protecting the kidneys), various internal organs are nourished. On the one hand, the hematopoietic function of hematopoietic organs is improved, directly utilizing nutrients to generate blood. On the other hand, the digestive system's ability to digest food and absorb and utilize nutrients is improved. Under good blood and qi circulation, a continuous supply of nutrients is provided to hematopoietic organs, thereby increasing the body's hematopoietic, blood-nourishing, and qi-tonifying effects. After the body obtains sufficient blood and qi, it will in turn nourish various organs, thus forming a virtuous cycle in the body's operation, full of vitality.

[0030] (2) Traditional Chinese medicine believes that the liver stores blood, governs the free flow of qi and blood, regulates emotions, and nourishes tendons and eyes. It plays a crucial role in maintaining the circulation of qi and blood, regulating emotions, and promoting digestion and absorption. If the liver's function of free flow of qi and blood is impaired, it can lead to irritability, anger, or depression. The kidneys store essence, regulate growth and aging, and govern reproductive function. Kidney deficiency can cause abnormal emotions such as fear and anxiety. Essence and blood are mutually generative, and the liver and kidneys work together to maintain reproductive, skeletal, and brain functions.

[0031] On the one hand, improved liver and kidney health reduces the frequency and duration of negative emotions such as anxiety, fear, and depression, leading to more stable emotions. This not only benefits the health of internal organs but also promotes faster sleep onset and longer deep sleep, thereby improving sleep function and quality. On the other hand, the effects of ingredients like black nightshade fruit, Kacip Fatimah, mulberry, Ophiopogon japonicus, L-threonate magnesium, and anserine peptide lower oxidative stress levels, alleviate neuroinflammation, protect neurons from damage, and allow damaged neurons to slowly repair themselves, effectively ensuring neuronal function and achieving a calming effect (studies have shown that oxidative stress is a significant factor in sleep disorders, and excessive reactive oxygen species can damage neurons). Furthermore, the effects of longan pulp, snow lotus culture, Ophiopogon japonicus, Ganoderma lucidum, L-threonate magnesium, γ-aminobutyric acid, and anserine peptide nourish nerves, regulate nerve metabolism, relieve irritability, and calm the mind, further facilitating rapid sleep onset. Furthermore, the effects of American ginseng, black nightshade fruit, red ginseng, ophiopogon japonicus, and ganoderma lucidum replenish lung qi, warm the lungs and resolve phlegm, and moisten the lungs to relieve cough, ensuring sufficient oxygen supply to the body during sleep and promoting relaxation. With the gradual improvement of organ health, replenishment of qi and blood, normalization of nerve metabolism, reduction of damage, and emotional stability, a significant improvement in sleep quality is inevitable.

[0032] (3) In addition, among the components provided by the present invention, American ginseng is cool in nature, longan pulp is warm in nature, black nightshade fruit is cold in nature, prepared rehmannia root is slightly warm in nature, snow lotus is warm in nature, red ginseng is warm in nature, mulberry is cold in nature, ophiopogon japonicus is slightly cold in nature, and lingzhi is neutral to warm in nature. Moreover, all the materials used in the present invention are medicinal and edible or beneficial Chinese herbal medicines or ingredients. The materials themselves have no toxic side effects. The components are prepared in proportion and combined with a specific preparation method, so that the prepared Yuling paste is mild in nature, neither cold nor hot. People with qi and blood deficiency and weak body can eat a certain amount of Yuling paste to achieve the effects of replenishing qi and blood, improving sleep quality, improving the health of internal organs and promoting the absorption and utilization of nutrients within a limited time, thereby fundamentally achieving the effect of strengthening the body and nourishing the source.

[0033] (4) The preparation method provided by the present invention makes full use of the advanced instruments and equipment brought about by the development of science and technology, effectively controls the temperature, and releases nutrients to the maximum extent through grinding and ultrasonic homogenization. While greatly improving the utilization of raw materials, it can also use advanced equipment to retain the active ingredients in the raw materials to the maximum extent through low temperature, and achieve the effect of sterilization.

[0034] The technical solution of the present invention will be further described in detail below with reference to the accompanying drawings and embodiments. Attached Figure Description

[0035] To more clearly illustrate the technical solutions of the embodiments of the present invention, the drawings used in the description of the embodiments of the present invention will be briefly introduced below. Obviously, the drawings described below are only some embodiments of the present invention. For those skilled in the art, other drawings can be obtained based on these drawings without creative effort.

[0036] Figure 1 These are mice with qi and blood deficiency that were fed with Yuling ointment (comparative example 1) for 4 weeks in the efficacy test 1 of this invention. Figure 2 The mice with qi and blood deficiency were fed the Yuling ointment prepared in Example 3 for 4 weeks in the efficacy test 1 of this invention. Detailed Implementation

[0037] The technical solution of the present invention will be further described below with reference to the accompanying drawings and embodiments.

[0038] To make the objectives, technical solutions, and advantages of this application clearer, more thorough, and more complete, the technical solutions of the present invention will be clearly and completely described below with reference to the accompanying drawings and embodiments. The following detailed descriptions are all illustrations of embodiments, intended to provide further detailed explanation of the present invention. Unless otherwise specified, all technical terms used in this invention have the same meaning as commonly understood by one of ordinary skill in the art to which this application pertains.

[0039] The instruments, equipment, and raw materials used in the embodiments were all obtained through commercial means.

[0040] Example 1 The specific steps for preparing 300g of Yuling Paste, which has the functions of replenishing qi and blood and regulating sleep, are as follows: S1. Remove dust and impurities from American ginseng, red ginseng, Kacip Fatimah, Rehmannia glutinosa, and Ganoderma lucidum, then freeze-dry them and sequentially slice, dice, crush, and grind them into powder. Freeze-dry the snow lotus culture, break it into small particles, grind it into powder, and pass each powder through an 80-mesh sieve. To prepare 300g of Yuling Paste with the function of replenishing qi and blood and regulating sleep, weigh the following sieved powders: 2.5g American ginseng, 6g red ginseng, 10g Kacip Fatimah, 7g Rehmannia glutinosa, 3g Ganoderma lucidum, and 28g snow lotus culture. Mix them in a mixer at 50℃ for 1 hour.

[0041] Add an equal volume of drinking water to the mixture and homogenize using ultrasound at 100 Hz for 30 minutes.

[0042] S2. Remove dust and impurities from the black nightshade fruit, ophiopogon japonicus, dried mulberry, and longan pulp, and cut them into small pieces (no larger than 0.125cm in volume). 3 Weigh the following ingredients: 5g of black nightshade fruit, 17g of ophiopogon japonicus, 20g of dried mulberry, and 25g of longan pulp. Mix them together and add an equal volume of drinking water. Then, homogenize the mixture using ultrasonication at 100Hz for 30 minutes.

[0043] S3. After ultrasonic homogenization of S1 and S2, stir and mix all components. Then add 6g of L-threonate magnesium, 5g of γ-aminobutyric acid and 10g of chemoresine, and stir and mix again. Stir the mixture at 65 (±2)℃ and 200 rpm for about 100 minutes until the water content of the mixture is about 60~75%. Cool to room temperature in an aseptic environment and bottle.

[0044] Example 2 A Yuling ointment with the functions of replenishing qi and blood and regulating sleep was prepared. The composition ratio and preparation method were the same as in Example 1, except that the ultrasonic homogenization conditions in steps S1 and S2 were 100Hz ultrasonic homogenization for 60 minutes.

[0045] Example 3 To prepare 300g of Yuling Ointment with functions of replenishing qi and blood and regulating sleep, the component ratio and preparation method were the same as in Example 2, except that in step S1, each powder was passed through an 80-mesh sieve and then mixed at 50°C for 2 hours.

[0046] Example 4 To prepare 300g of Yuling Ointment with functions of replenishing qi and blood and regulating sleep, the component ratio and preparation method were the same as in Example 2, except that in step S1, each powder was passed through an 80-mesh sieve and then mixed at 60°C for 1 hour.

[0047] Example 5 Yuling Paste, with the function of replenishing qi and blood and regulating sleep, was prepared in 300g using the same method as in Example 3. The amounts of each component were as follows: 2.5g American ginseng, 6g red ginseng, 10g Kacip Fatimah, 7g Rehmannia glutinosa, 3g Ganoderma lucidum, 28g snow lotus culture, 5g black nightshade fruit, 17g Ophiopogon japonicus, 20g dried mulberry, 25g longan pulp, 6g L-threonate magnesium, 5g γ-aminobutyric acid, and 5g serotonin.

[0048] Example 6 Yuling Paste, with the function of replenishing qi and blood and regulating sleep, was prepared in 300g using the same method as in Example 3. The amounts of each component were as follows: 2.5g American ginseng, 3g red ginseng, 10g Kacip Fatimah, 7g Rehmannia glutinosa, 3g Ganoderma lucidum, 28g snow lotus culture, 5g black nightshade fruit, 17g Ophiopogon japonicus, 20g dried mulberry, 25g longan pulp, 6g L-threonate magnesium, 5g γ-aminobutyric acid, and 10g goose muscle peptide.

[0049] Example 7 Yuling Paste, with the function of replenishing qi and blood and regulating sleep, was prepared in 300g using the same method as in Example 3. The amounts of each component were as follows: 2.5g American ginseng, 6g red ginseng, 10g Kacip Fatimah, 7g Rehmannia glutinosa, 3g Ganoderma lucidum, 28g snow lotus culture, 5g black nightshade fruit, 17g Ophiopogon japonicus, 10g dried mulberry, 25g longan pulp, 6g magnesium L-threonate, 5g γ-aminobutyric acid, and 10g gonioskin peptide.

[0050] Comparative Example 1 The ingredients list of the purchased Yuling Paste shows that it consists only of longan pulp and American ginseng.

[0051] Comparative Example 2 A Yuling ointment with the functions of replenishing qi and blood and regulating sleep was prepared. The composition ratio and preparation method were the same as in Example 2, except that the mixture in steps S1 and S3 was mixed at 100°C for 1 hour.

[0052] Comparative Example 3 A Yuling ointment with the functions of replenishing qi and blood and regulating sleep was prepared. The composition ratio and preparation method were the same as those of Comparative Example 2, except that the ultrasound step was omitted.

[0053] The component information of Examples 1 to 7 and Comparative Examples 1 to 3 is shown in Table 1.

[0054] Table 1. Component information in Examples 1-7 and Comparative Examples 1-3

[0055] Note: Examples in the table Example ,right Indicates a comparative proportion Effect Test 1 The Yuling ointment from Examples 1-7, Comparative Example 1 (purchased), and Comparative Example 2 and Comparative Example 3 (prepared) were subjected to mouse experiments, as detailed below: S1. Under sterile conditions, approximately 259 μL of blood (about 1 / 6 of the total blood volume) was collected from healthy 4-week-old Kunming mice (20±2g) via venous sampling to prepare a mouse model of qi and blood deficiency caused by massive blood loss. The qi and blood deficiency model mice were randomly divided into 10 groups, with 6 mice in each group, half male and half female.

[0056] S2. One group of mice was randomly selected and fed with Yuling Paste purchased from Examples 1 to 7, Comparative Example 1, and Comparative Example 3, respectively. Each mouse was fed 0.2g of Yuling Paste daily, with normal diet and water. Mice that were not subjected to modeling treatment and were fed normally served as the control group.

[0057] Four weeks later, the mice were weighed and ranked according to the density and shine of their fur. The results are shown in Table 2. Mice fed with the Yuling ointment prepared in Example 1 were designated as Group 1, and so on. A photograph of the mice in Group 1 is shown below. Figure 1 As shown, the mouse photographs of Example 3 group are as follows. Figure 2 As shown.

[0058] Table 2. Mouse body weight statistics

[0059] The results indicate that the Yuling ointment prepared in Examples 1-7 has a better effect on replenishing qi and blood than the Yuling ointment in the comparative examples. Extending the mixing time of the powder at 50°C or increasing it to 60°C in step S1 resulted in a better-effect Yuling ointment. This may be because a longer mixing time or a slightly higher processing temperature makes the powder material more potent, leading to better absorption and utilization of the active ingredients by the body. Comparing with Comparative Example 2, it can be seen that when the mixing temperature reaches 100°C (traditional Yuling ointment requires steaming for more than 40 hours, using steam generated from boiling water, the temperature of which is higher than 100°C), this may be one of the reasons why the effect in Comparative Example 1 differs significantly from that in Examples 2-4. Extending the ultrasonic homogenization time further improves the effect of the prepared Yuling ointment. Based on the results of Comparative Example 3, it is speculated that appropriately extending the ultrasonic homogenization time may facilitate the release of active ingredients.

[0060] Effect Test 2 The Yuling Ointment from Examples 1-7, Comparative Example 1 (purchased), and Comparative Example 2 and Comparative Example 3 (prepared) were used in volunteer trials, as detailed below: Twenty female patients aged 40-50 years old, diagnosed with Qi and Blood deficiency by Traditional Chinese Medicine, were recruited. All patients had no other underlying medical conditions. Before the trial, volunteers were informed of the composition of the Yuling Paste they would consume and signed an informed consent form. Each volunteer selected either the Yuling Paste prepared in the example case or the comparative case, or purchased by lottery.

[0061] For the first 10 days, take 5g / 60kg before breakfast and 5g / 60kg half an hour before bedtime daily. If no adverse reactions occur, for the next 20 days, take 10g / 60kg before breakfast and 10g / 60kg half an hour before bedtime daily. If adverse reactions occur, stop taking the medication immediately or reduce the dosage to 3g / 60kg before breakfast and 3g / 60kg half an hour before bedtime daily. During the trial, volunteers promised not to stay up late (falling asleep after 11:30 PM) and to wear a sleep monitoring bracelet while sleeping.

[0062] During the trial, patients paid attention to and recorded the information required in Table 2. The inventors compiled the recorded information every 10 days. The data statistics results for each volunteer are shown in Table 3. The number of times stool was formed was the total number of times during the period. The percentage of deep sleep (%) and the duration of deep sleep (minutes) were the average values ​​during the period.

[0063] Table 3 Volunteer Trial Data

[0064] Note: Examples in the table Example of consumption The prepared Jade Spirit Ointment has the effect of Indicates the proportion of consumption Jade Spirit Ointment (prepared or purchased) As can be seen from the statistical data in Table 3, the results of the experiment in Effect Test 2 are basically consistent with those in Effect Test 1. Furthermore, no adverse reactions were observed in any of the volunteers during the experiment, indicating that the Yuling plaster prepared using the appropriate proportions of each component and the matching preparation method is mild in nature, neither too cold nor too hot, and the dosage is also appropriately set.

[0065] Therefore, the Yuling Paste provided by this invention is composed of 13 kinds of medicinal and edible or beneficial Chinese herbal medicines or ingredients. The materials themselves have no toxic side effects. After the raw materials are prepared in proportion, the Yuling Paste is prepared by a specific preparation method. It is mild in nature, neither cold nor hot. People with qi and blood deficiency and weak body can achieve the effects of replenishing qi and blood, improving sleep quality, improving the health of internal organs and the absorption and utilization of nutrients within a limited time by consuming a certain amount of Yuling Paste. It fundamentally achieves the effect of strengthening the body and promoting the formation of a virtuous cycle in the body.

[0066] Finally, it should be noted that the above embodiments are only used to illustrate the technical solutions of the present invention and not to limit them. Although the present invention has been described in detail with reference to preferred embodiments, those skilled in the art should understand that modifications or equivalent substitutions can still be made to the technical solutions of the present invention, and these modifications or equivalent substitutions cannot cause the modified technical solutions to deviate from the spirit and scope of the technical solutions of the present invention.

Claims

1. A Yuling ointment with functions of replenishing qi and blood and regulating sleep, characterized in that, It is composed of the following components: American ginseng, longan pulp, black nightshade fruit, snow lotus culture, red ginseng, Kacip Fatimah, dried mulberry, prepared rehmannia root, magnesium L-threonate, γ-aminobutyric acid, Ophiopogon japonicus, Ganoderma lucidum and goose muscle peptide.

2. The Yuling Ointment with Qi-tonifying, blood-nourishing, and sleep-regulating functions according to claim 1, characterized in that, It consists of the following components by weight: 2-3 parts American ginseng, 3-6 parts red ginseng, 7-12 parts Kacip Fatimah, 5-8 parts Rehmannia glutinosa, 2-5 parts Ganoderma lucidum, 25-30 parts snow lotus culture, 2-8 parts black nightshade fruit, 15-20 parts Ophiopogon japonicus, 10-20 parts dried mulberry, 20-30 parts longan pulp, 3-8 parts magnesium L-threonate, 3-8 parts γ-aminobutyric acid and 5-10 parts anserine peptide.

3. The Yuling Ointment with Qi-tonifying, blood-nourishing, and sleep-regulating functions according to claim 1, characterized in that, It consists of the following components by weight: 2.5 parts American ginseng, 6 parts red ginseng, 10 parts Kacip Fatimah, 7 parts Rehmannia glutinosa, 3 parts Ganoderma lucidum, 28 parts snow lotus culture, 5 parts black nightshade fruit, 17 parts Ophiopogon japonicus, 20 parts dried mulberry, 25 parts longan pulp, 6 parts magnesium L-threonate, 5 parts γ-aminobutyric acid and 5 parts anserine peptide.

4. The Yuling Ointment with Qi-tonifying, blood-nourishing, and sleep-regulating functions according to claim 1, characterized in that, It consists of the following components by weight: 2.5 parts American ginseng, 3 parts red ginseng, 10 parts Kacip Fatimah, 7 parts Rehmannia glutinosa, 3 parts Ganoderma lucidum, 28 parts snow lotus culture, 5 parts black nightshade fruit, 17 parts Ophiopogon japonicus, 20 parts dried mulberry, 25 parts longan pulp, 6 parts magnesium L-threonate, 5 parts γ-aminobutyric acid and 10 parts anserine peptide.

5. The Yuling Ointment with Qi-tonifying, blood-nourishing, and sleep-regulating functions according to claim 1, characterized in that, It consists of the following components by weight: 2.5 parts American ginseng, 6 parts red ginseng, 10 parts Kacip Fatimah, 7 parts Rehmannia glutinosa, 3 parts Ganoderma lucidum, 28 parts snow lotus culture, 5 parts black nightshade fruit, 17 parts Ophiopogon japonicus, 10 parts dried mulberry, 25 parts longan pulp, 6 parts magnesium L-threonate, 5 parts γ-aminobutyric acid and 10 parts anserine peptide.

6. The preparation method of Yuling Ointment with Qi-tonifying, blood-nourishing, and sleep-regulating functions as described in any one of claims 1 to 5, characterized in that, The steps are as follows: S1. Remove dust and impurities from American ginseng, red ginseng, Kacip Fatima, and Ganoderma lucidum, then freeze-dry them and slice, cut into pieces, crush, grind into powder, and stir-fry at low temperature. Freeze-dry snow lotus culture and grind it into powder. Mix the low-temperature stir-fried combination with snow lotus culture powder and add drinking water for ultrasonic homogenization. S2. Remove dust and impurities from longan pulp, black nightshade fruit, ophiopogon japonicus, dried mulberry, and prepared rehmannia root, cut them into small pieces, and then add drinking water for ultrasonic homogenization. S3. After ultrasonic homogenization of S1 and S2, stir and mix all the components. Then add L-magnesium threonate, γ-aminobutyric acid and chemoresine, stir and mix again. Keep the mixture at 62~65℃ for 90~120min, and then bottle it aseptically.

7. The preparation method of Yuling Ointment with Qi-tonifying, blood-nourishing, and sleep-regulating functions according to claim 6, characterized in that: S1 is ground into powder and then sieved through an 80-mesh sieve; low-temperature frying involves mixing the sieved powders of each component at 50-65℃ for 1-2 hours; during ultrasonic homogenization, the volume ratio of drinking water added to the mixture is 1:

1.

8. The preparation method of Yuling Ointment with Qi-tonifying, blood-nourishing, and sleep-regulating functions according to claim 6, characterized in that: The small pieces cut from S2 should not exceed 0.125 cm in volume. 3 The volume ratio of drinking water added to the mixture was 1:1; the ultrasonic homogenization in S1 and S2 was performed at 100 Hz for 30-40 min.

9. The preparation method of Yuling Ointment with Qi-tonifying, blood-nourishing, and sleep-regulating functions according to claim 6, characterized in that: In S3, maintain the temperature at 62-65℃ for 90-120 minutes to reduce the water content of the mixture to 60-75%.

10. The application of Yuling Paste, as described in any one of claims 1 to 5, for the preparation of food products that replenish qi and blood and regulate sleep.

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