Traditional Chinese medicine composition as well as preparation method and application thereof

Abstract

The invention relates to the technical field of traditional Chinese medicines, and particularly discloses a traditional Chinese medicine composition as well as a preparation method and application thereof. The traditional Chinese medicine composition comprises the following traditional Chinese medicine raw material components in parts by weight: 15-20 parts of astragalus membranaceus; 10-15 parts of prepared rehmannia root; 5-10 parts of Chinese magnoliavine fruit; 5-10 parts of lotus leaves; and 1-5 parts of honey-fried licorice root. The traditional Chinese medicine composition also comprises acanthopanax or rhodiola rosea; in addition, the traditional Chinese medicine composition can also comprise one or a mixture of more than one of cistanche, raspberry, rehmannia, rhizoma polygonati, fingered citron, spina date seed, mulberry, red date, medlar, radix fici simplicissimae, fish collagen peptide, corn peptide and elastin. The traditional Chinese medicine composition disclosed by the invention has the effects of resisting fatigue, improving sleep and / or enhancing immunity; therefore, the traditional Chinese medicine composition has important application value when being used as an active ingredient for preparing medicines or health-care products with the effects of resisting fatigue, improving sleep and / or enhancing immunity.

Description

Technical Field

[0001] This invention relates to the field of traditional Chinese medicine technology, specifically to a traditional Chinese medicine composition, its preparation method, and its application. Background Technology

[0002] With the accelerated pace of modern life, increased work pressure, and the aging population, sub-health has become a significant issue affecting public health. Chronic fatigue, sleep disorders, and weakened immunity often intertwine, forming a vicious cycle of "fatigue-insomnia-immunosuppression," significantly reducing individual quality of life and increasing the risk of infectious diseases, metabolic syndrome, and even mental health issues.

[0003] Currently, Western medicine lacks specific drugs for treating chronic fatigue, and clinical practice often employs symptomatic interventions, such as central nervous system stimulants, antidepressants, vitamin supplements, or hormone medications. However, these therapies often suffer from uncertain efficacy, significant side effects (such as insomnia, palpitations, and dependence), and a high relapse rate after discontinuation, making it difficult to meet the needs for safe, long-term, and holistic health management.

[0004] Traditional Chinese medicine (TCM) has a profound theoretical foundation and rich practical experience in the field of fatigue relief. The *Huangdi Neijing* (Yellow Emperor's Inner Classic) states that "exertion depletes qi" and that "the spleen is the source of qi and blood production," emphasizing the close relationship between fatigue and dysfunction of the spleen, kidneys, and heart. Traditional formulas such as Buzhong Yiqi Tang (Tonifying the Middle and Benefiting Qi Decoction), Shiquan Dabu Tang (Ten-Ingredient Tonic Decoction), and Shengmai San (Life-Generating Powder) demonstrate certain anti-fatigue effects in clinical practice by tonifying qi and blood, strengthening the spleen, and benefiting the kidneys.

[0005] Therefore, developing a traditional Chinese medicine composition with anti-fatigue effects using traditional Chinese herbs as raw materials has significant application value. In particular, developing a composition that not only improves fatigue but also alleviates secondary symptoms caused by fatigue (such as sleep disorders and weakened immunity) is of even greater importance. Summary of the Invention

[0006] In order to overcome at least one technical problem existing in the prior art, the present invention provides a traditional Chinese medicine composition, its preparation method and application.

[0007] The technical solution of the present invention is as follows: This invention first provides a traditional Chinese medicine composition comprising the following traditional Chinese medicine raw material components in parts by weight: Astragalus membranaceus 15-20 parts; Rehmannia glutinosa 10-15 parts; Schisandra chinensis 5-10 parts; Nelumbo nucifera leaf 5-10 parts; Glycyrrhiza uralensis 1-5 parts.

[0008] The inventors discovered in their research that a traditional Chinese medicine composition prepared from raw materials such as Astragalus membranaceus, Rehmannia glutinosa, Schisandra chinensis, lotus leaf, and Glycyrrhiza uralensis has an anti-fatigue effect.

[0009] Preferably, the traditional Chinese medicine composition comprises the following traditional Chinese medicine raw material components in parts by weight: Astragalus membranaceus 18-20 parts; Rehmannia glutinosa 10-12 parts; Schisandra chinensis 8-10 parts; Nelumbo nucifera leaf 8-10 parts; Glycyrrhiza uralensis 3-4 parts.

[0010] Preferably, the traditional Chinese medicine composition comprises the following traditional Chinese medicine raw material components in parts by weight: Astragalus membranaceus 18 parts; Rehmannia glutinosa 12 parts; Schisandra chinensis 8 parts; Nelumbo nucifera leaf 8 parts; Glycyrrhiza uralensis 4 parts.

[0011] Preferably, the traditional Chinese medicine composition further includes Rhodiola rosea.

[0012] Further research by the inventors revealed that the anti-fatigue effect of a traditional Chinese medicine composition prepared by adding Rhodiola rosea to traditional Chinese medicine raw materials such as Astragalus membranaceus, Rehmannia glutinosa, Schisandra chinensis, lotus leaf, and prepared licorice root was further improved compared to a traditional Chinese medicine composition prepared using the same raw materials.

[0013] Preferably, the weight of Rhodiola rosea is 8-12 parts.

[0014] Most preferably, the amount of Rhodiola rosea is 10 parts by weight.

[0015] Preferably, the traditional Chinese medicine composition further includes Acanthopanax senticosus.

[0016] Further research by the inventors revealed that the anti-fatigue effect of a traditional Chinese medicine composition prepared by adding Acanthopanax senticosus to raw materials such as Astragalus membranaceus, Rehmannia glutinosa, Schisandra chinensis, lotus leaf, and prepared licorice root was further improved compared to a traditional Chinese medicine composition prepared using raw materials such as Astragalus membranaceus, Rehmannia glutinosa, Schisandra chinensis, lotus leaf, and prepared licorice root.

[0017] Preferably, the weight of Acanthopanax senticosus is 8-12 parts.

[0018] Preferably, the weight of Acanthopanax senticosus is 10 parts.

[0019] Preferably, the traditional Chinese medicine composition further includes Rhodiola rosea and Acanthopanax senticosus.

[0020] Further research by the inventors revealed that the anti-fatigue effect of a herbal composition prepared by adding Rhodiola rosea and Acanthopanax senticosus to traditional Chinese medicine raw materials such as Astragalus membranaceus, Rehmannia glutinosa, Schisandra chinensis, Nelumbo nucifera leaf, and Glycyrrhiza uralensis was significantly higher than that of a herbal composition prepared by adding Rhodiola rosea or Acanthopanax senticosus alone. Furthermore, the simultaneous addition of Rhodiola rosea and Acanthopanax senticosus to the same raw materials synergistically enhanced the anti-fatigue effect of the prepared herbal composition.

[0021] Preferably, the weight parts of Rhodiola rosea and Acanthopanax senticosus are 8-12 parts.

[0022] Most preferably, the weight parts of Rhodiola rosea and Acanthopanax senticosus are 10 parts.

[0023] Furthermore, the herbal raw materials in the aforementioned herbal composition can also be compounded with other raw materials that have anti-fatigue, sleep-improving, and / or immune-enhancing effects.

[0024] Preferably, the raw materials for preparing the traditional Chinese medicine composition further include one or more of the following: Cistanche deserticola, raspberry, Rehmannia glutinosa, Polygonatum sibiricum, Citrus medica, Ziziphus jujuba var. spinosa, mulberry, red date, wolfberry, Ficus hirta, fish collagen peptide, corn peptide, and elastin.

[0025] Preferably, the preparation method of the traditional Chinese medicine composition includes the following steps: Take Chinese medicinal materials, mix them, and then pulverize them to obtain a mixed powder of Chinese medicinal materials; The mixed powder of Chinese herbal raw materials is extracted with an ethanol-water solution. After extraction, the extract is separated, concentrated and dried to obtain the extract. The extract is then used to obtain the Chinese herbal composition.

[0026] Preferably, the ratio of the mixed powder of Chinese herbal raw materials to the aqueous ethanol solution is 1 kg: 5-10 L.

[0027] Preferably, the ratio of the mixed powder of Chinese herbal raw materials to the aqueous ethanol solution is 1 kg: 8 L.

[0028] Preferably, the volume fraction of ethanol in the aqueous ethanol solution is 50-80%.

[0029] Most preferably, the volume fraction of ethanol in the aqueous ethanol solution is 70%.

[0030] Preferably, the extraction is performed by heating and reflux extraction, and the extraction time is 1-2 hours.

[0031] Preferably, the method for preparing the traditional Chinese medicine composition is characterized by comprising the following steps: Take Astragalus membranaceus, Rehmannia glutinosa (processed), Schisandra chinensis, Nelumbo nucifera leaf, and Glycyrrhiza uralensis (processed), mix them together, and then pulverize them to obtain mixed powder of Chinese medicinal materials A; Take the mixed powder of Chinese herbal raw materials A and extract it with ethanol aqueous solution. After the extraction is completed, separate the extract, concentrate and dry the extract to obtain extract A. Rhodiola rosea and Acanthopanax senticosus were mixed and pulverized to obtain mixed powder of Chinese medicinal materials B; Take the mixed powder of Chinese herbal raw materials B and extract it with a mixed solvent. After the extraction is completed, separate the extract, concentrate and dry the extract to obtain extract B. The traditional Chinese medicine composition is obtained by mixing mixture A and mixture B evenly.

[0032] Preferably, the ratio of the mixed powder of Chinese herbal raw materials A to the aqueous ethanol solution is 1 kg: 5-10 L.

[0033] Preferably, the ratio of the mixed powder of Chinese herbal raw materials A to the aqueous ethanol solution is 1 kg: 8 L.

[0034] Preferably, the volume fraction of ethanol in the aqueous ethanol solution is 50-80%.

[0035] Most preferably, the volume fraction of ethanol in the aqueous ethanol solution is 70%.

[0036] Preferably, the ratio of the mixed powder of Chinese herbal raw materials B to the mixed solvent is 1kg:5-10L.

[0037] Preferably, the ratio of the mixed powder of Chinese herbal raw materials B to the mixed solvent is 1 kg: 8 L.

[0038] Preferably, the mixed solvent consists of cyclohexane and acetone.

[0039] In further research, the inventors discovered that the selection of extraction solvents for Rhodiola rosea and Acanthopanax senticosus is crucial in the preparation of the traditional Chinese medicine composition of this invention. Compared with the traditional Chinese medicine composition prepared by extracting Rhodiola rosea and Acanthopanax senticosus alone with a mixed solvent composed of cyclohexane and acetone, the traditional Chinese medicine composition prepared by extracting Rhodiola rosea and Acanthopanax senticosus together with Astragalus membranaceus, Rehmannia glutinosa, Schisandra chinensis, Nelumbo nucifera leaf and Glycyrrhiza uralensis in an ethanol-water solution can further significantly improve the anti-fatigue effect of the prepared traditional Chinese medicine composition.

[0040] Preferably, the volume ratio of cyclohexane to acetone is 1:1-3.

[0041] Most preferably, the volume ratio of cyclohexane to acetone is 1:2.

[0042] Preferably, the extraction is performed by heating and reflux extraction, and the extraction time is 1-2 hours.

[0043] The present invention also provides an application of the above-mentioned traditional Chinese medicine composition in the preparation of products with anti-fatigue effects.

[0044] The present invention also provides an application of the above-mentioned traditional Chinese medicine composition in the preparation of a product with sleep-improving effects.

[0045] The present invention also provides an application of the above-mentioned traditional Chinese medicine composition in the preparation of products with immune-enhancing effects.

[0046] Preferably, the product is a medicine or health product.

[0047] Beneficial Effects: This invention provides a novel traditional Chinese medicine composition, and research shows that this composition has anti-fatigue effects. Furthermore, from the perspective of traditional Chinese medicine principles, Astragalus membranaceus and Acanthopanax senticosus tonify the spleen and lung qi, strengthen the kidneys and enhance the body's anti-fatigue ability; Rehmannia glutinosa nourishes the kidneys and replenishes essence, nourishes blood and calms the mind, nourishing the body's vital energy from the "foundation of life," improving insomnia and restlessness; Schisandra chinensis astringes the lungs and nourishes the kidneys, calms the mind and soothes the spirit, restrains excessive yang to aid sleep, and protects vital energy; Nelumbo nucifera leaf clears heat and eliminates dampness, invigorates the spleen and stomach, prevents stagnation from tonifying, ensuring the tonic is not cloying, and allows clear yang to rise to promote mental clarity; Rhodiola rosea invigorates qi and blood, combats fatigue and unblocks the meridians, improving fatigue and sleep disorders caused by qi deficiency and blood stasis; Glycyrrhiza uralensis tonifies the middle jiao and invigorates qi, harmonizes the other herbs, assists the heart and restores the pulse, and synergistically enhances the overall regulatory function of the formula. The composition of this invention achieves anti-fatigue, sleep improvement, and enhanced immunity through dual tonification of the spleen and kidneys, simultaneous regulation of qi and blood, nourishment of the mind and spirit, and orderly balance of clear and turbid substances.

[0048] Since the traditional Chinese medicine composition described in this invention has anti-fatigue, sleep-improving and / or immune-enhancing effects, using the traditional Chinese medicine composition described in this invention as an active ingredient to prepare drugs or health products with anti-fatigue, sleep-improving and / or immune-enhancing effects has important application value. Detailed Implementation

[0049] The present invention will be further explained below with reference to specific embodiments, but the embodiments do not limit the scope of protection of the present invention.

[0050] Example 1: Preparation of Traditional Chinese Medicine Composition The raw materials of traditional Chinese medicine are composed of the following parts by weight: Astragalus membranaceus 18 parts; Rehmannia glutinosa 12 parts; Schisandra chinensis 8 parts; Nelumbo nucifera leaf 8 parts; Glycyrrhiza uralensis 4 parts.

[0051] Preparation method: (1) Take Chinese herbal raw materials such as Astragalus membranaceus, Rehmannia glutinosa, Schisandra chinensis, lotus leaf and prepared licorice root, mix them and then pulverize them to obtain a mixed powder of Chinese herbal raw materials; (2) Take the mixed powder of Chinese herbal raw materials, heat it to boiling with ethanol aqueous solution and extract for 1.5h. After the extraction is completed, separate the extract, concentrate and dry the extract to obtain the extract, and take the extract to obtain the Chinese herbal composition; wherein, the ratio of the mixed powder of Chinese herbal raw materials to the ethanol aqueous solution is 1kg:8L; the volume fraction of ethanol in the ethanol aqueous solution is 70%.

[0052] Example 2 Preparation of Traditional Chinese Medicine Composition Composition of Chinese medicinal materials by weight: Astragalus membranaceus 18 parts; Rehmannia glutinosa 12 parts; Schisandra chinensis 8 parts; Nelumbo nucifera leaf 8 parts; Rhodiola rosea 10 parts; Glycyrrhiza uralensis 4 parts.

[0053] Preparation method: (1) Take Chinese herbal raw materials such as Astragalus membranaceus, Rehmannia glutinosa, Schisandra chinensis, Nelumbo nucifera leaf, Rhodiola rosea and Glycyrrhiza uralensis, mix them and then pulverize them to obtain a mixed powder of Chinese herbal raw materials; (2) Take the mixed powder of Chinese herbal raw materials, heat it to boiling with ethanol aqueous solution and extract for 1.5h. After the extraction is completed, separate the extract, concentrate and dry the extract to obtain the extract, and take the extract to obtain the Chinese herbal composition; wherein, the ratio of the mixed powder of Chinese herbal raw materials to the ethanol aqueous solution is 1kg:8L; the volume fraction of ethanol in the ethanol aqueous solution is 70%.

[0054] Example 3 Preparation of Traditional Chinese Medicine Composition Composition of Chinese medicinal materials by weight: Astragalus membranaceus 18 parts; Rehmannia glutinosa 12 parts; Schisandra chinensis 8 parts; Nelumbo nucifera leaf 8 parts; Acanthopanax senticosus 10 parts; Glycyrrhiza uralensis 4 parts.

[0055] Preparation method: (1) Take Chinese herbal raw materials such as Astragalus membranaceus, Rehmannia glutinosa, Schisandra chinensis, Nelumbo nucifera leaf, Acanthopanax senticosus and Glycyrrhiza uralensis, mix them and then pulverize them to obtain a mixed powder of Chinese herbal raw materials; (2) Take the mixed powder of Chinese herbal raw materials, heat it to boiling with ethanol aqueous solution and extract for 1.5h. After the extraction is completed, separate the extract, concentrate and dry the extract to obtain the extract, and take the extract to obtain the Chinese herbal composition; wherein, the ratio of the mixed powder of Chinese herbal raw materials to the ethanol aqueous solution is 1kg:8L; the volume fraction of ethanol in the ethanol aqueous solution is 70%.

[0056] Example 4 Preparation of Traditional Chinese Medicine Composition Composition of Chinese medicinal materials by weight: Astragalus membranaceus 18 parts; Rehmannia glutinosa 12 parts; Schisandra chinensis 8 parts; Nelumbo nucifera leaf 8 parts; Rhodiola rosea 10 parts; Acanthopanax senticosus 10 parts; Glycyrrhiza uralensis 4 parts.

[0057] Preparation method: (1) Take Chinese herbal raw materials such as Astragalus membranaceus, Rehmannia glutinosa, Schisandra chinensis, Nelumbo nucifera leaf, Rhodiola rosea, Acanthopanax senticosus and Glycyrrhiza uralensis, mix them and then pulverize them to obtain a mixed powder of Chinese herbal raw materials; (2) Take the mixed powder of Chinese herbal raw materials, heat it to boiling with ethanol aqueous solution and extract for 1.5h. After the extraction is completed, separate the extract, concentrate and dry the extract to obtain the extract, and take the extract to obtain the Chinese herbal composition; wherein, the ratio of the mixed powder of Chinese herbal raw materials to the ethanol aqueous solution is 1kg:8L; the volume fraction of ethanol in the ethanol aqueous solution is 70%.

[0058] Example 5 Preparation of Traditional Chinese Medicine Composition Composition of Chinese medicinal materials by weight: Astragalus membranaceus 18 parts; Rehmannia glutinosa 12 parts; Schisandra chinensis 8 parts; Nelumbo nucifera leaf 8 parts; Rhodiola rosea 10 parts; Acanthopanax senticosus 10 parts; Glycyrrhiza uralensis 4 parts.

[0059] Preparation method: (1) Take Astragalus membranaceus, Rehmannia glutinosa, Schisandra chinensis, lotus leaf and prepared licorice root, mix them together and then pulverize them to obtain mixed powder of Chinese herbal raw materials A; (2) Take the mixed powder of Chinese herbal raw materials A, heat it to boiling with an ethanol aqueous solution and extract for 1.5 h. After extraction, separate the extract, concentrate and dry the extract to obtain extract A; wherein, the material-liquid ratio of the mixed powder of Chinese herbal raw materials A to the ethanol aqueous solution is 1 kg: 8 L; the volume fraction of ethanol in the ethanol aqueous solution is 70%; (3) Take Rhodiola rosea and Acanthopanax senticosus, mix them and then crush them to obtain mixed powder of Chinese herbal raw materials B; (4) Take the mixed powder of Chinese herbal raw materials B, heat it to boiling with a mixed solvent and extract it for 1.5 hours. After the extraction is completed, separate the extract, concentrate and dry the extract to obtain extract B; wherein, the ratio of the mixed powder of Chinese herbal raw materials B to the mixed solvent is 1 kg: 8 L; the mixed solvent is composed of cyclohexane and acetone in a volume ratio of 1:2. (5) The traditional Chinese medicine composition is obtained by mixing mixture A and mixture B evenly.

[0060] Comparative Example 1: Preparation of Traditional Chinese Medicine Composition Composition of Chinese medicinal materials by weight: Astragalus membranaceus 18 parts; Rehmannia glutinosa 12 parts; Schisandra chinensis 8 parts; Nelumbo nucifera leaf 8 parts; Rhodiola rosea 10 parts; Acanthopanax senticosus 10 parts; Glycyrrhiza uralensis 4 parts.

[0061] Preparation method: (1) Take Astragalus membranaceus, Rehmannia glutinosa, Schisandra chinensis, lotus leaf and prepared licorice root, mix them together and then pulverize them to obtain mixed powder of Chinese herbal raw materials A; (2) Take the mixed powder of Chinese herbal raw materials A, heat it to boiling with an ethanol aqueous solution and extract for 1.5 h. After extraction, separate the extract, concentrate and dry the extract to obtain extract A; wherein, the material-liquid ratio of the mixed powder of Chinese herbal raw materials A to the ethanol aqueous solution is 1 kg: 8 L; the volume fraction of ethanol in the ethanol aqueous solution is 70%; (3) Take Rhodiola rosea and Acanthopanax senticosus, mix them and then crush them to obtain mixed powder of Chinese herbal raw materials B; (4) Take the mixed powder of Chinese herbal raw materials B, heat it to boiling with solvent and extract it for 1.5 hours. After the extraction is completed, separate the extract, concentrate and dry the extract to obtain extract B; wherein, the ratio of the mixed powder of Chinese herbal raw materials B to the solvent is 1 kg: 8 L; the solvent is cyclohexane. (5) The traditional Chinese medicine composition is obtained by mixing mixture A and mixture B evenly.

[0062] Comparative Example 2: Preparation of Traditional Chinese Medicine Composition Composition of Chinese medicinal materials by weight: Astragalus membranaceus 18 parts; Rehmannia glutinosa 12 parts; Schisandra chinensis 8 parts; Nelumbo nucifera leaf 8 parts; Rhodiola rosea 10 parts; Acanthopanax senticosus 10 parts; Glycyrrhiza uralensis 4 parts.

[0063] Preparation method: (1) Take Astragalus membranaceus, Rehmannia glutinosa, Schisandra chinensis, lotus leaf and prepared licorice root, mix them together and then pulverize them to obtain mixed powder of Chinese herbal raw materials A; (2) Take the mixed powder of Chinese herbal raw materials A, heat it to boiling with an ethanol aqueous solution and extract for 1.5 h. After extraction, separate the extract, concentrate and dry the extract to obtain extract A; wherein, the material-liquid ratio of the mixed powder of Chinese herbal raw materials A to the ethanol aqueous solution is 1 kg: 8 L; the volume fraction of ethanol in the ethanol aqueous solution is 70%; (3) Take Rhodiola rosea and Acanthopanax senticosus, mix them and then crush them to obtain mixed powder of Chinese herbal raw materials B; (4) Take the mixed powder of Chinese herbal raw materials B, heat it to boiling with a solvent and extract it for 1.5 hours. After the extraction is completed, separate the extract, concentrate and dry the extract to obtain extract B; wherein, the ratio of the mixed powder of Chinese herbal raw materials B to the solvent is 1 kg: 8 L; the solvent is acetone. (5) The traditional Chinese medicine composition is obtained by mixing mixture A and mixture B evenly.

[0064] Comparative Example 3: Preparation of Traditional Chinese Medicine Composition Composition of Chinese medicinal materials by weight: Astragalus membranaceus 18 parts; Rehmannia glutinosa 12 parts; Schisandra chinensis 8 parts; Nelumbo nucifera leaf 8 parts; Rhodiola rosea 10 parts; Acanthopanax senticosus 10 parts; Glycyrrhiza uralensis 4 parts.

[0065] Preparation method: (1) Take Astragalus membranaceus, Rehmannia glutinosa, Schisandra chinensis, lotus leaf and prepared licorice root, mix them together and then pulverize them to obtain mixed powder of Chinese herbal raw materials A; (2) Take the mixed powder of Chinese herbal raw materials A, heat it to boiling with an ethanol aqueous solution and extract for 1.5 h. After extraction, separate the extract, concentrate and dry the extract to obtain extract A; wherein, the material-liquid ratio of the mixed powder of Chinese herbal raw materials A to the ethanol aqueous solution is 1 kg: 8 L; the volume fraction of ethanol in the ethanol aqueous solution is 70%; (3) Take Rhodiola rosea and Acanthopanax senticosus, mix them and then crush them to obtain mixed powder of Chinese herbal raw materials B; (4) Take the mixed powder of Chinese herbal raw materials B, heat it to boiling with a solvent and extract it for 1.5 hours. After the extraction is completed, separate the extract, concentrate and dry the extract to obtain extract B; wherein, the ratio of the mixed powder of Chinese herbal raw materials B to the solvent is 1 kg: 8 L; the solvent is methanol. (5) The traditional Chinese medicine composition is obtained by mixing mixture A and mixture B evenly.

[0066] Comparative Example 4: Preparation of Traditional Chinese Medicine Composition Composition of Chinese medicinal materials by weight: Astragalus membranaceus 18 parts; Rehmannia glutinosa 12 parts; Schisandra chinensis 8 parts; Nelumbo nucifera leaf 8 parts; Rhodiola rosea 10 parts; Acanthopanax senticosus 10 parts; Glycyrrhiza uralensis 4 parts.

[0067] Preparation method: (1) Take Astragalus membranaceus, Rehmannia glutinosa, Schisandra chinensis, lotus leaf and prepared licorice root, mix them together and then pulverize them to obtain mixed powder of Chinese herbal raw materials A; (2) Take the mixed powder of Chinese herbal raw materials A, heat it to boiling with an ethanol aqueous solution and extract for 1.5 h. After extraction, separate the extract, concentrate and dry the extract to obtain extract A; wherein, the material-liquid ratio of the mixed powder of Chinese herbal raw materials A to the ethanol aqueous solution is 1 kg: 8 L; the volume fraction of ethanol in the ethanol aqueous solution is 70%; (3) Take Rhodiola rosea and Acanthopanax senticosus, mix them and then crush them to obtain mixed powder of Chinese herbal raw materials B; (4) Take the mixed powder of Chinese herbal raw materials B, heat it to boiling with a mixed solvent and extract it for 1.5 hours. After the extraction is completed, separate the extract, concentrate and dry the extract to obtain extract B; wherein, the ratio of the mixed powder of Chinese herbal raw materials B to the mixed solvent is 1 kg: 8 L; the mixed solvent is composed of cyclohexane and methanol with a volume ratio of 1:2. (5) The traditional Chinese medicine composition is obtained by mixing mixture A and mixture B evenly.

[0068] Experimental Example: Fatigue Resistance Experiment One hundred and twenty male KM mice weighing 20±2g were randomly divided into 10 groups of 12 mice each. The experimental group was administered the test Chinese medicine composition by gavage once a day at a dose of 10mg / kg. The control group was administered an equal volume of physiological saline by gavage. After four weeks of continuous gavage, a lead wire weighing 10% of the mice's body weight was attached to the tail of each group of mice. The mice were then placed in a water tank at a temperature of 25℃ and swam. The swimming time of each group of mice was recorded. The swimming time refers to the time from when the mouse enters the water until it is exhausted and its head is completely submerged and cannot float. The results are shown in Table 1.

[0069] Table 1. Experimental results of the anti-fatigue effects of the traditional Chinese medicine composition of this invention. The Chinese medicine composition to be tested Swimming time (min) control group - 71.2±4.4 Experimental group 1 The traditional Chinese medicine composition prepared in Example 1 80.6±4.9 Experimental group 2 The traditional Chinese medicine composition prepared in Example 2 86.1±5.7 Experimental group 3 The traditional Chinese medicine composition prepared in Example 3 90.2±5.5 Experimental group 4 The traditional Chinese medicine composition prepared in Example 4 107.5±7.1 Experimental group 5 The traditional Chinese medicine composition prepared in Example 5 139.8±10.4 Experimental group 6 The traditional Chinese medicine composition prepared in Comparative Example 1 113.5±8.8 Experimental group 7 The traditional Chinese medicine composition prepared in Comparative Example 2 120.4±8.5 Experimental group 8 The traditional Chinese medicine composition prepared in Comparative Example 3 117.6±8.1 Experimental group 9 The traditional Chinese medicine composition prepared in Comparative Example 4 118.9±9.3 As can be seen from the experimental results in Table 1, the swimming time of mice in experimental group 1 was significantly longer than that in the control group. This indicates that the traditional Chinese medicine composition prepared by the present invention using raw materials such as Astragalus membranaceus, Rehmannia glutinosa, Schisandra chinensis, lotus leaf and Glycyrrhiza uralensis has an anti-fatigue effect.

[0070] As can be seen from the experimental results in Table 1, the swimming time of mice in experimental groups 2 and 3 was significantly longer than that of mice in experimental group 1. This indicates that the anti-fatigue effect of the traditional Chinese medicine composition prepared by adding Rhodiola rosea or Acanthopanax senticosus to the raw materials such as Astragalus membranaceus, Rehmannia glutinosa, Schisandra chinensis, Nelumbo nucifera, and Glycyrrhiza uralensis is further improved compared to the traditional Chinese medicine composition prepared by adding the raw materials such as Astragalus membranaceus, Rehmannia glutinosa, Schisandra chinensis, Nelumbo nucifera, and Glycyrrhiza uralensis.

[0071] As shown in Table 1, the swimming time of mice in experimental group 4 was significantly longer than that in experimental groups 2 and 3. This indicates that the anti-fatigue effect of the herbal composition prepared by adding Rhodiola rosea and Acanthopanax senticosus to the raw materials such as Astragalus membranaceus, Rehmannia glutinosa, Schisandra chinensis, Nelumbo nucifera, and Glycyrrhiza uralensis is significantly higher than that of the herbal composition prepared by adding Rhodiola rosea or Acanthopanax senticosus alone. Furthermore, the simultaneous addition of Rhodiola rosea and Acanthopanax senticosus to the raw materials such as Astragalus membranaceus, Rehmannia glutinosa, Schisandra chinensis, Nelumbo nucifera, and Glycyrrhiza uralensis can synergistically enhance the anti-fatigue effect of the prepared herbal composition.

[0072] As can be seen from the experimental results in Table 1, the swimming time of mice in experimental group 5 was significantly higher than that in experimental group 4. This indicates that the selection of extraction solvents for Rhodiola rosea and Acanthopanax senticosus is crucial in the preparation of the traditional Chinese medicine composition of this invention. The traditional Chinese medicine composition prepared by extracting Rhodiola rosea and Acanthopanax senticosus alone using a mixed solvent composed of cyclohexane and acetone can significantly improve the anti-fatigue effect of the prepared traditional Chinese medicine composition compared to the traditional Chinese medicine composition prepared by extracting Rhodiola rosea and Acanthopanax senticosus together with Astragalus membranaceus, Rehmannia glutinosa, Schisandra chinensis, Nelumbo nucifera leaf and Glycyrrhiza uralensis in an ethanol aqueous solution.

[0073] As shown in Table 1, although the swimming time of mice in experimental groups 6-9 was improved compared to that in experimental group 4, the improvement was much smaller than that in experimental group 5. This indicates that only when Rhodiola rosea and Acanthopanax senticosus are extracted individually using a mixed solvent composed of cyclohexane and acetone can the anti-fatigue effect be significantly enhanced compared to the anti-fatigue effect obtained by extracting Rhodiola rosea and Acanthopanax senticosus together with Astragalus membranaceus, Rehmannia glutinosa, Schisandra chinensis, Nelumbo nucifera leaf, and Glycyrrhiza uralensis in an ethanol-water solution. However, the anti-fatigue effect of the anti-fatigue effect obtained by extracting Rhodiola rosea and Acanthopanax senticosus individually using a mixed solvent composed of other solvents cannot be significantly enhanced.

[0074] Example 6 Preparation of Traditional Chinese Medicine Composition Composition of Chinese medicinal materials by weight: Astragalus membranaceus 18 parts; Rehmannia glutinosa 12 parts; Schisandra chinensis 8 parts; Nelumbo nucifera leaf 8 parts; Glycyrrhiza uralensis (processed) 4 parts; Cistanche deserticola 10 parts; Rubus idaeus 10 parts; Rehmannia glutinosa 8 parts; Polygonatum sibiricum 8 parts; Citrus medica 8 parts; Ziziphus jujuba var. spinosa 5 parts; Morus alba 5 parts; Jujube 5 parts; Lycium barbarum 5 parts; Ficus hirta 5 parts.

[0075] Preparation method: (1) Take the following Chinese medicinal materials: Astragalus membranaceus, Rehmannia glutinosa, Schisandra chinensis, Nelumbo nucifera leaf, Glycyrrhiza uralensis (processed), Cistanche deserticola, Rubus idaeus, Rehmannia glutinosa, Polygonatum sibiricum, Citrus medica, Ziziphus jujuba var. spinosa, Morus alba, Jujube, Lycium barbarum, and Ficus hirta, mix them together and then pulverize them to obtain a mixed powder of Chinese medicinal materials. (2) Take the mixed powder of Chinese herbal raw materials, heat it to boiling with ethanol aqueous solution and extract for 1.5h. After the extraction is completed, separate the extract, concentrate and dry the extract to obtain the extract, and take the extract to obtain the Chinese herbal composition; wherein, the ratio of the mixed powder of Chinese herbal raw materials to the ethanol aqueous solution is 1kg:8L; the volume fraction of ethanol in the ethanol aqueous solution is 70%.

[0076] Example 7 Preparation of the composition The composition is obtained by mixing the traditional Chinese medicine composition with fish collagen peptides evenly; wherein the weight ratio of the traditional Chinese medicine composition to the fish collagen peptides is 1:3; the traditional Chinese medicine composition is the composition prepared according to any one of Examples 1-6.

[0077] Example 8 Preparation of the composition The composition is obtained by mixing the traditional Chinese medicine composition with elastin evenly; wherein the weight ratio of the traditional Chinese medicine composition to elastin is 1:3; the traditional Chinese medicine composition is the composition prepared according to any one of Examples 1-6.

[0078] Example 9 Preparation of the composition The composition is obtained by mixing the traditional Chinese medicine composition with fish collagen peptide, corn peptide and elastin evenly; wherein the weight ratio of the traditional Chinese medicine composition to fish collagen peptide, corn peptide and elastin is 1:1:1:1; the traditional Chinese medicine composition is the composition prepared according to any one of Examples 1-6.

Claims

1. A traditional Chinese medicine composition, characterized in that, The traditional Chinese medicine raw material components comprise the following weight parts: Radix Astragali 15-20 parts; Radix Rehmanniae Preparata 10-15 parts; Schisandra chinensis 5-10 parts; Lotus leaf 5-10 parts; Radix Glycyrrhizae Preparata 1-5 parts.

2. The traditional Chinese medicine composition according to claim 1, characterized in that, The traditional Chinese medicine raw material components comprise the following weight parts: Radix Astragali 18-20 parts; Radix Rehmanniae Preparata 10-12 parts; Schisandra chinensis 8-10 parts; Lotus leaf 8-10 parts; Radix Glycyrrhizae Preparata 3-4 parts.

3. The traditional Chinese medicine composition according to claim 1, characterized in that, The traditional Chinese medicine raw material components comprise the following weight parts: Radix Astragali 18 parts; Radix Rehmanniae Preparata 12 parts; Schisandra chinensis 8 parts; Lotus leaf 8 parts; Radix Glycyrrhizae Preparata 4 parts.

4. The traditional Chinese medicine composition according to claim 1, characterized in that, The traditional Chinese medicine composition further comprises Rhodiola.

5. The traditional Chinese medicine composition according to claim 1, characterized in that, The traditional Chinese medicine composition further comprises Acanthopanax.

6. The preparation method of the traditional Chinese medicine composition according to any one of claims 1-5, characterized in that, The steps comprise: Traditional Chinese medicine raw materials are taken, mixed, and then pulverized to obtain a traditional Chinese medicine raw material mixed powder; The traditional Chinese medicine raw material mixed powder is extracted with an ethanol aqueous solution, and the extraction is separated after the extraction is completed. The extraction is concentrated and dried to obtain an extract, and the extract is taken to obtain the traditional Chinese medicine composition.

7. Use of the traditional Chinese medicine composition of any one of claims 1-5 in the preparation of a product having an anti-fatigue effect.

8. Use of the traditional Chinese medicine composition of any one of claims 1-5 in the preparation of a product having a sleep improving effect.

9. Use of the traditional Chinese medicine composition of any one of claims 1-5 in the preparation of a product having an immune enhancing effect.