A hydrogel composition for three-dimensional culture of tumor cells and a preparation method thereof
By combining GelMA and PLGA-PEG-PLGA, the pH of the tumor cell culture microenvironment is dynamically adjusted and expandable pores are formed, solving the problem that existing hydrogel materials cannot simultaneously meet the requirements of biomimetic biological activity and acidic microenvironment in tumor cell culture, and achieving a three-dimensional culture effect with high stability and high accuracy.
Patent Information
- Application Number
- CN202610281739.7
- Authority / Receiving Office
- CN · China
- Patent Type
- Patents(China)
- Current Assignee / Owner
- Filing Date
- 2026-03-10
- Publication Date
- 2026-09-18
- Estimated Expiration
- 2046-03-10
AI Technical Summary
Existing hydrogel materials cannot simultaneously meet the triple requirements of biomimetic bioactivity, dynamic mechanical microenvironment, and characteristic acidic microenvironment, resulting in poor batch stability of in vitro 3D models, poor maintenance of tumor phenotype, and low accuracy of drug screening.
A combination of methacrylamide gelatin (GelMA) and polylactic acid glycol copolymer (PLGA-PEG-PLGA) was used to generate lactic acid through the biodegradation of PLGA-PEG-PLGA, dynamically adjusting the microenvironment pH to 6.5-6.9 to simulate the acidic conditions of tumors, and forming an expandable porous structure during the degradation process.
It enables long-term stable culture of tumor cells, provides a biomimetic three-dimensional growth space, supports the formation and invasion of tumor spheroids, and improves the accuracy of drug screening and cell survival rate.