Miniature vacuum device of synchrotron radiation infrared spectrum for biological sample experiment

Abstract

The invention belongs to the technical field of biological sample experiments, and particularly relates to a synchrotron radiation infrared spectrum miniature vacuum device for biological sample experiments, which comprises a vacuumizing mechanism and a sealing cover, the vacuumizing mechanism comprises a tray, a sealing box, a support pad, a gas pipe, a miniature nitrogen cylinder, a gas flow controller, a miniature vacuum pump and a control valve, a sealing box is arranged at the top of the tray, a supporting pad is arranged at the bottom of an inner cavity of the sealing box, the left side and the right side of the sealing box are communicated with air pipes, and the outer end of the air pipe on the right side is connected with a miniature nitrogen cylinder. Or the sealing box is slowly filled with the nitrogen in the micro nitrogen cylinder, so that the sealing box is filled with the nitrogen, the synchrotron radiation infrared spectrum test can be conveniently carried out, result bias is avoided, a treatment mode can be selected according to requirements, and the application range is expanded.

Description

Technical Field

[0001] This invention relates to the field of biological sample experimentation technology, specifically a miniature vacuum device for biological sample experiments using synchrotron infrared spectroscopy. Background Technology

[0002] Biological samples typically refer to the flowers, leaves, stems, roots, and seeds of plants; the body fluids (such as urine, blood, saliva, bile, gastric juice, lymph, and other secretions of organisms) of animals (including humans), hair, muscles, and some tissues and organs (such as thymus, pancreas, liver, lungs, brain, stomach, and kidneys); and various microorganisms. Biological samples can be detected by synchrotron infrared spectroscopy, which can provide spectral data with a high signal-to-noise ratio. However, biological samples (such as proteins and cells) are prone to dehydration and denaturation in a conventional vacuum environment, leading to structural damage.

[0003] Because biological samples are easily affected by oxygen, carbon dioxide, and water vapor during experiments, leading to protein denaturation, synchrotron infrared spectroscopy experiments can produce result bias. In order to use infrared spectroscopy to truly reflect the characteristics of biological samples, it is urgent to design a vacuum device to reduce the influence of oxygen, carbon dioxide, water vapor, etc. Therefore, a miniature vacuum device for synchrotron infrared spectroscopy in biological sample experiments is proposed. Summary of the Invention

[0004] The purpose of this section is to outline some aspects of the embodiments of the present invention and to briefly describe some preferred embodiments. Simplifications or omissions may be made in this section, as well as in the abstract and title of this application, to avoid obscuring the purpose of these documents; however, such simplifications or omissions should not be construed as limiting the scope of the invention.

[0005] In view of the problems existing in the above and / or existing biological sample experiments, the present invention is proposed.

[0006] Therefore, the purpose of this invention is to provide a miniature vacuum device for synchrotron infrared spectroscopy in biological sample experiments. This device uses a miniature vacuum pump to create a vacuum within the sealed chamber, enabling infrared detection. Alternatively, nitrogen from a miniature nitrogen cylinder can be slowly introduced into the sealed chamber to fill it with nitrogen, facilitating synchrotron infrared spectroscopy experiments, avoiding result bias, and allowing for selection of processing methods according to requirements, thus expanding its application range.

[0007] To address the aforementioned technical problems, according to one aspect of the present invention, the present invention provides the following technical solution:

[0008] A miniature vacuum device for synchrotron infrared spectroscopy in biological sample experiments, comprising:

[0009] The vacuuming mechanism includes a tray, a sealed box, a support pad, a gas tube, a miniature nitrogen cylinder, a gas flow controller, a miniature vacuum pump, a control valve, and a gas drying tube. The sealed box is mounted on the top of the tray, and the support pad is mounted on the bottom of the inner cavity of the sealed box. Gas tubes are connected to both the left and right sides of the sealed box. The outer end of the gas tube on the right side is connected to the miniature nitrogen cylinder. A gas flow controller is mounted on the gas tube on the right side. A miniature vacuum pump is mounted at the end of the gas tube on the left side. Control valves are mounted on both the left and right gas tubes. A gas drying tube is mounted on the gas tube on the right side.

[0010] A sealing cap is located on top of the sealed box.

[0011] As a preferred embodiment of the miniature vacuum device for biological sample experiments using synchrotron infrared spectroscopy as described in this invention, the support pad includes a first glass slide, an isolation pad, and a second glass slide, with the isolation pad disposed on top of the first glass slide and the second glass slide disposed on top of the isolation pad.

[0012] As a preferred embodiment of the miniature vacuum device for biological sample experiments using synchrotron infrared spectroscopy as described in this invention, the isolation pad is composed of multiple stacked pads, with the thickness of the multiple pads increasing layer by layer.

[0013] As a preferred embodiment of the miniature vacuum device for biological sample experiments using synchrotron infrared spectroscopy as described in this invention, a heat-conducting coil is provided on the right-side gas pipe, a horn tube is provided on the left-side gas pipe, and a constant-temperature water bath is provided outside the heat-conducting coil.

[0014] As a preferred embodiment of the miniature vacuum device for biological sample experiments using synchrotron infrared spectroscopy as described in this invention, the following features: a transmission mechanism is connected inside the horn tube; the transmission mechanism includes a drive shaft, an impeller, a drive bevel gear, a transmission shaft, a driven bevel gear, a turntable, an eccentric shaft, and a connecting arm; the drive shaft is rotatably connected inside the horn tube; an impeller is located at one end of the drive shaft inside the horn tube; a drive bevel gear is located at one end of the drive shaft inside the sealed chamber; the transmission shaft is rotatably connected to the left side wall of the inner cavity of the sealed chamber; a driven bevel gear meshing with the drive bevel gear is located on the transmission shaft; turntables are located at both ends of the transmission shaft; eccentric shafts are located on the outer sides of both turntables; the eccentric shafts are located on the upper and lower sides of the turntables, respectively; connecting arms are connected to both eccentric shafts; and fan mechanisms are connected to the connecting arms.

[0015] As a preferred embodiment of the miniature vacuum device for biological sample experiments using synchrotron infrared spectroscopy as described in this invention, the sealed box has slide rails on both the front and rear side walls, and evenly distributed hinge seats on both the upper and lower sides of the slide rails. The fan mechanism includes a slide rod, a support plate, a connecting shaft, a fan plate, a sliding groove, and a hinge shaft. The slide rod is slidably connected to the slide rail, and the left end of the slide rod is rotatably connected to the connecting arm. Evenly distributed connecting shafts are provided on the slide rod. Sliding grooves for connecting shafts are opened at the top and bottom of the fan plate, and hinge shafts for connecting hinge seats are provided at both the upper and lower ends of the fan plate.

[0016] As a preferred embodiment of the miniature vacuum device for biological sample experiments using synchrotron infrared spectroscopy as described in this invention, each slide rod is connected to a connecting rod and a wind shield at its right end. One end of the connecting rod is fixed to the slide rod, and the other end of the connecting rod is provided with a wind shield.

[0017] As a preferred embodiment of the miniature vacuum device for biological sample experiments using synchrotron infrared spectroscopy as described in this invention, the following features: a positioning mechanism is connected to the drive shaft; the positioning mechanism includes a support rod, a support shaft, a clamping plate, a strip hole, a support spring, a clamping pad, and a pull rope; the support rods are symmetrically arranged on the front and rear sides of the left side wall of the sealed chamber; a support shaft is provided at the end of each support rod; a strip hole is opened at the left end of the clamping plate for rotatable connection with the support shaft; the clamping pad is connected to the right end of the clamping plate via a support spring; and a pull rope is connected between the left end of the clamping plate and the drive shaft.

[0018] As a preferred embodiment of the miniature vacuum device for biological sample experiments using synchrotron infrared spectroscopy as described in this invention, the sealing cover includes a cover plate, track grooves, a cleaning strip, a first fixing plate, a corrugated pipe, a connecting pipe, a second fixing plate, and a return spring. An air hole is provided on the rear side wall of the inner cavity of the sealing box. The rear end of the cover plate is hinged to the sealing box. Track grooves are provided on both the left and right sides of the top of the cover plate. A cleaning strip is slidably connected between the track grooves. A first fixing plate is provided on the top of the cleaning strip. A corrugated pipe is provided on the right end of the first fixing plate. The right end of the corrugated pipe is connected to the air hole via the connecting pipe. A second fixing plate is provided on the right end of the top of the cover plate. The right end of the corrugated pipe is fixed to the second fixing plate. A return spring is provided between the cleaning strip and the track groove.

[0019] As a preferred embodiment of the miniature vacuum device for biological sample experiments using synchrotron infrared spectroscopy as described in this invention, wherein: in the working state of the transmission mechanism, the slide rods on both sides move in opposite directions.

[0020] Compared with existing technologies, this invention places biological samples in a sealed box and uses a micro vacuum pump to create a vacuum inside the box, enabling infrared detection. Alternatively, nitrogen from a micro nitrogen cylinder is slowly introduced into the sealed box to fill it with nitrogen, facilitating synchrotron radiation infrared spectroscopy experiments, avoiding result bias, and allowing for selection of processing methods according to needs, thus expanding the application range. Attached Figure Description

[0021] To more clearly illustrate the technical solutions of the embodiments of the present invention, the present invention will be described in detail below with reference to the accompanying drawings and detailed embodiments. Obviously, the drawings described below are only some embodiments of the present invention. For those skilled in the art, other drawings can be obtained based on these drawings without creative effort. Wherein:

[0022] Figure 1 This is a schematic diagram of the axial structure of the present invention;

[0023] Figure 2 This is a schematic diagram of the internal structure of the present invention;

[0024] Figure 3 This is a schematic diagram of the vacuum pumping mechanism of the present invention;

[0025] Figure 4 This is a schematic diagram of the support pad structure of the present invention;

[0026] Figure 5 This is a schematic diagram of the sealing cap structure of the present invention;

[0027] Figure 6 This is a schematic diagram of the transmission mechanism structure of the present invention;

[0028] Figure 7 This is an enlarged structural schematic diagram of the fan mechanism of the present invention;

[0029] Figure 8 This is a schematic diagram of the positioning mechanism of the present invention.

[0030] In the diagram: 100 Vacuuming mechanism, 110 Tray, 120 Sealing box, 121 Slide rail, 122 Hinge, 123 Air vent, 130 Support pad, 131 First glass slide, 132 Isolation pad, 133 Second glass slide, 140 Gas pipe, 141 Heat-conducting coil, 142 Horn tube, 150 Miniature nitrogen cylinder, 160 Gas flow controller, 170 Miniature vacuum pump, 180 Control valve, 190 Gas drying tube, 200 Sealing cap, 210 Cover plate, 220 Track groove, 230 Cleaning strip, 240 First fixing plate, 250 Corrugated pipe, 260 Connecting pipe, 270 Second fixing plate. 280 Return spring, 300 Constant temperature water bath, 400 Transmission mechanism, 410 Drive shaft, 420 Impeller, 430 Drive bevel gear, 440 Transmission shaft, 450 Driven bevel gear, 460 Turntable, 470 Eccentric shaft, 480 Connecting arm, 500 Fan mechanism, 510 Slide rod, 520 Support plate, 530 Connecting shaft, 540 Fan plate, 550 Slide groove, 560 Hinge shaft, 570 Connecting rod, 580 Wind shield, 600 Positioning mechanism, 610 Support rod, 620 Support shaft, 630 Clamping plate, 640 Strip hole, 650 Support spring, 660 Clamping pad, 670 Pull rope. Detailed Implementation

[0031] To make the above-mentioned objects, features and advantages of the present invention more apparent and understandable, the specific embodiments of the present invention will be described in detail below with reference to the accompanying drawings.

[0032] Many specific details are set forth in the following description in order to provide a full understanding of the invention. However, the invention may also be practiced in other ways different from those described herein, and those skilled in the art can make similar extensions without departing from the spirit of the invention. Therefore, the invention is not limited to the specific embodiments disclosed below.

[0033] Secondly, the present invention is described in detail with reference to the schematic diagrams. When detailing the embodiments of the present invention, for ease of explanation, the cross-sectional views illustrating the device structure may be partially enlarged, not according to the usual scale. Furthermore, the schematic diagrams are merely examples and should not limit the scope of protection of the present invention. In addition, actual fabrication should include three-dimensional spatial dimensions of length, width, and depth.

[0034] To make the objectives, technical solutions, and advantages of the present invention clearer, the embodiments of the present invention will be described in further detail below with reference to the accompanying drawings.

[0035] This invention provides a miniature vacuum device for synchrotron infrared spectroscopy in biological sample experiments. It uses a miniature vacuum pump to evacuate the sealed chamber to achieve infrared detection, or it slowly fills the sealed chamber with nitrogen from a miniature nitrogen cylinder to facilitate synchrotron infrared spectroscopy experiments, avoids result bias, and allows for selection of processing methods according to needs, thus expanding the application range. Its main components include: a vacuum pumping mechanism 100 and a sealing cover 200.

[0036] The vacuuming mechanism 100 includes a tray 110, a sealed box 120, a support pad 130, a gas pipe 140, a miniature nitrogen cylinder 150, a gas flow controller 160, a miniature vacuum pump 170, and a control valve 180. The sealed box 120 is set on the top of the tray 110, and the support pad 130 is set at the bottom of the inner cavity of the sealed box 120. The left and right sides of the sealed box 120 are connected to the gas pipe 140. The outer end of the right gas pipe 140 is connected to the miniature nitrogen cylinder 150. The gas flow controller 160 is set on the right gas pipe 140, and the miniature vacuum pump 170 is set at the end of the left gas pipe 140. The control valve 180 is set on both the left and right gas pipes 140, and the gas drying pipe 190 is set on the right gas pipe 140.

[0037] The biological sample is placed at the top center of the support pad 130. The control valve 180 at the micro vacuum pump 170 is turned on, and the control valve 180 at the micro nitrogen cylinder 150 is turned off. The micro vacuum pump 170 works to pump air and create a vacuum in the sealed box 120.

[0038] Open the control valve 180 at the micro vacuum pump 170 and simultaneously open the control valve 180 at the micro nitrogen cylinder 150. While pumping air, the micro nitrogen cylinder 150 fills the sealed box 120 with nitrogen to remove the air from the sealed box 120. At the same time, nitrogen is injected into the sealed box 120 to balance the air pressure, so that the sealed box 120 is filled with nitrogen. The gas is dried through the gas drying tube 190 to reduce internal moisture. The gas drying tube 190 is located close to the sealed box 120 and can effectively remove moisture from the gas tube 140 to keep it dry. Then close the control valves 180 on both sides to prevent the internal nitrogen from being lost.

[0039] Nitrogen can help maintain the activity of biological samples under certain conditions. The core principle is to use the inertness of nitrogen to isolate oxygen and moisture, thereby reducing factors that cause sample inactivation, such as oxidation and microbial contamination.

[0040] The sealing cover 200 is located on the top of the sealing box 120, and the sealing box 120 is sealed by the sealing cover 200. A sealing gasket is provided between the sealing cover 200 and the sealing box 120 to prevent leakage and ensure the stability of the vacuum state.

[0041] The support pad 130 includes a first glass slide 131, an isolation pad 132, and a second glass slide 133. The isolation pad 132 is placed on top of the first glass slide 131, and the second glass slide 133 is placed on top of the isolation pad 132. The first glass slide 131 is a BAF2 glass slide with a thickness of 1 mm. Ten isolation pads 132 are placed on the first glass slide 131, with a thickness of 0.1 to 1 mm. The number of pads can be selected according to the needs, which is convenient for adjustment. The second glass slide 133 is placed on the isolation pad 132. The second glass slide 133 is a BAF2 glass slide with a thickness of 1 mm. Holes are drilled on both sides of the second glass slide 133 for fixing and connecting pipes of a miniature vacuum device and a simple nitrogen gas supply device.

[0042] Because the nitrogen gas discharged from the miniature nitrogen cylinder 150 is at a low temperature when it rapidly expands from a high-pressure environment to a low-pressure environment, when the low-temperature gas directly enters the sealed box 120, the water vapor remaining in the sealed box 120 will condense and adhere to the inner wall of the sealed box 120 and be difficult to discharge. Therefore, a heat-conducting coil 141 is installed on the right gas pipe 140, and a horn tube 142 is installed on the left gas pipe 140. A constant temperature water bath 300 is installed outside the heat-conducting coil 141.

[0043] The gas flowing through the heat-conducting coil 141 is heated by the constant temperature water bath 300, so that the constant temperature gas is introduced into the sealed box 120, which avoids water vapor condensation and adhesion to the inner wall of the sealed box 120, and facilitates the complete discharge of internal water vapor.

[0044] Because nitrogen is introduced through a single port, it cannot quickly and evenly fill the sealed box 120. Therefore, a transmission mechanism 400 is connected inside the horn tube 142. The transmission mechanism 400 includes a drive shaft 410, an impeller 420, a drive bevel gear 430, a transmission shaft 440, a driven bevel gear 450, a turntable 460, an eccentric shaft 470, and a connecting arm 480. The drive shaft 410 is rotatably connected inside the horn tube 142, and the impeller 420 is located at one end of the drive shaft 410 inside the horn tube 142. A drive bevel gear 430 is provided at one end inside the sealed box 120. A transmission shaft 440 is rotatably connected to the left side wall of the inner cavity of the sealed box 120. A driven bevel gear 450 that meshes with the drive bevel gear 430 is provided on the transmission shaft 440. Turntables 460 are provided at both ends of the transmission shaft 440. An eccentric shaft 470 is provided on the outer side of both turntables 460. The eccentric shafts 470 are located on the upper and lower sides of the turntables 460 respectively. A connecting arm 480 is connected to each eccentric shaft 470. A fan mechanism 500 is connected to the connecting arm 480.

[0045] Specifically, when the internal air is extracted, the internal airflow quickly passes through the flow horn tube 142 to form a pressurized flow. The pressurized airflow drives the impeller 420 to rotate. The impeller 420 drives the drive bevel gear 430 to rotate through the drive shaft 410. The drive bevel gear 430 meshes and drives the driven bevel gear 450 to rotate. The driven bevel gear 450 drives the turntable 460 to rotate through the transmission shaft 440. The turntable 460 drives the connecting arm 480 to rock through the eccentric shaft 470. The connecting arm 480 drives the fan mechanism 500 to work, fanning the internal airflow so that nitrogen can be quickly and evenly filled.

[0046] Specifically, the front and rear side walls of the inner cavity of the sealed box 120 are provided with slide rails 121, and the upper and lower sides of the slide rails 121 are provided with evenly distributed hinge seats 122. The fan mechanism 500 includes a slide rod 510, a support plate 520, a connecting shaft 530, a fan plate 540, a slide groove 550 and a hinge shaft 560. The slide rod 510 is slidably connected to the slide rail 121, and the left end of the slide rod 510 is rotatably connected to the connecting arm 480. The slide rod 510 is provided with evenly distributed connecting shafts 530. The top and bottom of the fan plate 540 are provided with slide grooves 550 that connect to the connecting shafts 530. The upper and lower ends of the fan plate 540 are provided with hinge shafts 560 that connect to the hinge seats 122.

[0047] When the connecting arm 480 moves, it pulls the slide bar 510 to move. The two slide bars 510 on both sides move in opposite directions. When the slide bar 510 moves, it drives the fan plate 540 to swing back and forth on the hinge seat 122 through the connecting shaft 530. The back and forth swinging fan plate 540 drives the airflow to make nitrogen evenly fill the sealed box 120.

[0048] Since nitrogen gas is discharged into the sealed box 120 through the gas pipe 140, the discharge direction is unidirectional and cannot be evenly filled. Therefore, the right end of the slide rod 510 is connected to the connecting rod 570 and the wind shield 580. One end of the connecting rod 570 is fixed to the slide rod 510, and the other end of the connecting rod 570 is equipped with the wind shield 580. When the two slide rods 510 move in opposite directions, the wind shield 580 alternately blocks the outlet of the gas pipe 140. When the front side is blocked, the gas pipe 140 exhausts from the rear side, and when the rear side is blocked, the gas pipe 140 exhausts from the front side, so that the airflow is alternately discharged into the sealed box 120, changing the exhaust direction and making the nitrogen gas evenly fill the sealed box 120.

[0049] Since the biological sample needs to be placed in the center, a positioning mechanism 600 is connected to the drive shaft 440. The positioning mechanism 600 includes a support rod 610, a support shaft 620, a clamping plate 630, a slotted hole 640, a support spring 650, a clamping pad 660, and a pull rope 670. The support rods 610 are symmetrically arranged on the front and rear sides of the left side wall of the inner cavity of the sealing box 120. The support shaft 620 is provided at the end of each support rod 610. The left end of the clamping plate 630 has a slotted hole 640 that is rotatably connected to the support shaft 620. The right end of the clamping plate 630 is connected to the clamping pad 660 through the support spring 650. The pull rope 670 is connected between the left end of the clamping plate 630 and the drive shaft 440.

[0050] When the drive shaft 440 rotates, it winds the pull rope 670. When the pull rope 670 winds, it pulls one end of the clamping plate 630, causing the clamping plate 630 to rotate on the support shaft 620, so that the two clamping plates 630 move toward the center of the biological sample.

[0051] Since external light source illumination is required, the sealing cover 200 includes a cover plate 210, a track groove 220, a cleaning strip 230, a first fixing plate 240, a corrugated pipe 250, a connecting pipe 260, a second fixing plate 270, and a return spring 280. An air hole 123 is provided on the rear side wall of the inner cavity of the sealing box 120. The rear end of the cover plate 210 is hinged to the sealing box 120. Track grooves 220 are provided on both the left and right sides of the top of the cover plate 210. The cleaning strip 230 is slidably connected between the track grooves 220. A first fixing plate 240 is provided on the top of the cleaning strip 230. A corrugated pipe 250 is provided on the right end of the first fixing plate 240. The right end of the corrugated pipe 250 is connected to the air hole 123 via the connecting pipe 260. A second fixing plate 270 is provided on the right end of the top of the cover plate 210. The right end of the corrugated pipe 250 is fixed to the second fixing plate 270. A return spring 280 is provided between the cleaning strip 230 and the track groove 220.

[0052] During the evacuation process, a negative pressure is formed inside the sealed box 120, which causes the bellows 250 to retract. The retracted bellows 250 drives the cleaning strip 230 to slide on the track groove 220, and the sliding cleaning strip 230 is used to clean the top of the cover plate 210.

[0053] In specific usage;

[0054] When using the vacuum detection method, the biological sample is placed at the top center of the support pad 130. The control valve 180 at the micro vacuum pump 170 is turned on, and the control valve 180 at the micro nitrogen cylinder 150 is turned off. The micro vacuum pump 170 works to evacuate the air, creating a vacuum inside the sealed box 120. During vacuuming, a negative pressure is formed inside the sealed box 120, causing the bellows 250 to retract. The retracted bellows 250 drives the cleaning strip 230 to slide on the track groove 220. The sliding cleaning strip 230 is used to clean the top of the cover plate 210, making it easier for the light source to illuminate the experiment.

[0055] When using the nitrogen-filled detection method, open the control valve 180 at the micro vacuum pump 170 and simultaneously open the control valve 180 at the micro nitrogen cylinder 150. While evacuating the air, the micro nitrogen cylinder 150 fills the sealed box 120 with nitrogen, evacuating the air from the sealed box 120. At the same time, nitrogen is injected into the sealed box 120 to balance the air pressure, so that the sealed box 120 is filled with nitrogen. The gas is then dried through the gas drying tube 190 to reduce internal moisture. The gas drying tube 190 is located close to the sealed box 120 and can effectively remove moisture from the gas tube 140 to keep it dry. After that, close the control valves 180 on both sides to prevent the internal nitrogen from being lost.

[0056] The gas flowing through the heat-conducting coil 141 is heated by a constant-temperature water bath 300, allowing the constant-temperature gas to enter the sealed box 120. This prevents water vapor condensation and adhesion to the inner wall of the sealed box 120, facilitating the complete removal of internal water vapor. When the internal air is extracted, the internal airflow rapidly passes through the flow horn tube 142, creating a pressurized flow. The pressurized airflow drives the impeller 420 to rotate. The impeller 420 drives the drive bevel gear 430 to rotate via the drive shaft 410. The drive bevel gear 430 meshes with and drives the driven bevel gear 450 to rotate. The driven bevel gear 450 is driven by the transmission shaft... 440 drives the turntable 460 to rotate, and the turntable 460 drives the connecting arm 480 to rock through the eccentric shaft 470. The connecting arm 480 drives the fan mechanism 500 to work, fanning the internal airflow so that nitrogen can be quickly and evenly filled. When the drive shaft 440 rotates, it winds the pull rope 670. When the pull rope 670 winds, it pulls one end of the clamping plate 630, causing the clamping plate 630 to rotate on the support shaft 620, so that the two clamping plates 630 move towards the biological sample in the center. During the detection, the installation position of the corrugated pipe 250 is moved to the side of the cover plate 210 to avoid blocking the external infrared light and affecting the detection.

[0057] Two detection methods are used to effectively expand the detection range.

[0058] Although the present invention has been described above with reference to embodiments, various modifications can be made and components can be replaced with equivalents without departing from the scope of the invention. In particular, as long as there is no structural conflict, the features in the disclosed embodiments can be combined with each other in any manner. The lack of an exhaustive description of these combinations in this specification is merely for the sake of brevity and resource conservation. Therefore, the present invention is not limited to the specific embodiments disclosed herein, but includes all technical solutions falling within the scope of the claims.

Claims

1. A miniature vacuum device for synchrotron infrared spectroscopy in biological sample experiments, characterized in that, include: The vacuum mechanism (100) includes a tray (110), a sealed box (120), a support pad (130), a gas pipe (140), a miniature nitrogen cylinder (150), a gas flow controller (160), a miniature vacuum pump (170), a control valve (180), and a gas drying tube (190). The sealed box (120) is installed on the top of the tray (110), and the support pad (130) is installed at the bottom of the inner cavity of the sealed box (120). The left and right sides of the sealed box (120) are connected to the gas pipe (140). The outer end of the right gas pipe (140) is connected to the miniature nitrogen cylinder (150). The gas flow controller (160) is installed on the right gas pipe (140). The miniature vacuum pump (170) is installed at the end of the left gas pipe (140). The control valve (180) is installed on both the left and right gas pipes (140), and the gas drying tube (190) is installed on the right gas pipe (140). A sealing cap (200) is disposed on top of the sealing box (120).

2. The miniature vacuum device for synchrotron infrared spectroscopy in biological sample experiments according to claim 1, characterized in that, The support pad (130) includes a first glass slide (131), an isolation pad (132), and a second glass slide (133). The isolation pad (132) is disposed on the top of the first glass slide (131), and the second glass slide (133) is disposed on the top of the isolation pad (132).

3. The miniature vacuum device for synchrotron infrared spectroscopy in biological sample experiments according to claim 2, characterized in that, The isolation pad (132) is composed of multiple pads stacked together, with the thickness of the multiple pads increasing layer by layer.

4. The miniature vacuum device for synchrotron infrared spectroscopy in biological sample experiments according to claim 1, characterized in that, A heat-conducting coil (141) is provided on the right-side air pipe (140), and a horn tube (142) is provided on the left-side air pipe (140). A constant temperature water tank (300) is provided outside the heat-conducting coil (141).

5. A miniature vacuum device for synchrotron infrared spectroscopy in biological sample experiments according to claim 4, characterized in that, A transmission mechanism (400) is connected inside the horn tube (142). The transmission mechanism (400) includes a drive shaft (410), an impeller (420), a drive bevel gear (430), a transmission shaft (440), a driven bevel gear (450), a turntable (460), an eccentric shaft (470), and a connecting arm (480). The drive shaft (410) is rotatably connected inside the horn tube (142). The impeller (420) is located at one end of the drive shaft (410) inside the horn tube (142), and the drive shaft (410) is located at the other end of the drive shaft (410) inside the sealed box (120). A bevel gear (430) is provided. The drive shaft (440) is rotatably connected to the left side wall of the inner cavity of the sealed box (120). A driven bevel gear (450) that meshes with the drive bevel gear (430) is provided on the drive shaft (440). Turntables (460) are provided at both ends of the drive shaft (440). An eccentric shaft (470) is provided on the outer side of both ends of the turntables (460). The eccentric shafts (470) are located on the upper and lower sides of the turntables (460) respectively. A connecting arm (480) is connected to each eccentric shaft (470). A fan mechanism (500) is connected to the connecting arm (480).

6. A miniature vacuum device for synchrotron infrared spectroscopy in biological sample experiments according to claim 5, characterized in that, The sealing box (120) has slide rails (121) on both the front and rear sides of the inner cavity. The slide rails (121) have evenly distributed hinge seats (122) on both the upper and lower sides. The fan mechanism (500) includes a slide rod (510), a support plate (520), a connecting shaft (530), a fan plate (540), a sliding groove (550), and a hinge shaft (560). The slide rod (510) is slidably connected to the slide rail (121). The left end of the slide rod (510) is rotatably connected to the connecting arm (480). The slide rod (510) has evenly distributed connecting shafts (530). The top and bottom of the fan plate (540) are provided with sliding grooves (550) that connect to the connecting shafts (530). The upper and lower ends of the fan plate (540) are provided with hinge shafts (560) that connect to the hinge seats (122).

7. A miniature vacuum device for synchrotron infrared spectroscopy in biological sample experiments according to claim 6, characterized in that, Each slide rod (510) is connected to a connecting rod (570) and a wind shield (580) at its right end. One end of the connecting rod (570) is fixed to the slide rod (510), and the other end of the connecting rod (570) is provided with a wind shield (580).

8. A miniature vacuum device for synchrotron infrared spectroscopy in biological sample experiments according to claim 5, characterized in that, A positioning mechanism (600) is connected to the drive shaft (440). The positioning mechanism (600) includes a support rod (610), a support shaft (620), a clamping plate (630), a strip hole (640), a support spring (650), a clamping pad (660), and a pull rope (670). The support rod (610) is symmetrically arranged on the front and rear sides of the left side wall of the inner cavity of the sealed box (120). The support shaft (620) is provided at the end of each support rod (610). The left end of the clamping plate (630) is provided with a strip hole (640) that is rotatably connected to the support shaft (620). The right end of the clamping plate (630) is connected to the clamping pad (660) through the support spring (650). The pull rope (670) is connected between the left end of the clamping plate (630) and the drive shaft (440).

9. A miniature vacuum device for synchrotron infrared spectroscopy in biological sample experiments according to claim 1, characterized in that, The sealing cover (200) includes a cover plate (210), a track groove (220), a cleaning strip (230), a first fixing plate (240), a corrugated pipe (250), a connecting pipe (260), a second fixing plate (270), and a return spring (280). The rear wall of the inner cavity of the sealing box (120) is provided with an air hole (123). The rear end of the cover plate (210) is hinged to the sealing box (120). Track grooves (220) are provided on both the left and right sides of the top of the cover plate (210), and the track grooves (220) slide between each other. A cleaning strip (230) is connected, a first fixing plate (240) is provided on the top of the cleaning strip (230), a corrugated pipe (250) is provided on the right end of the first fixing plate (240), the right end of the corrugated pipe (250) is connected to the air hole (123) through a connecting pipe (260), a second fixing plate (270) is provided on the top right end of the cover plate (210), the right end of the corrugated pipe (250) is fixed on the second fixing plate (270), and a return spring (280) is provided between the cleaning strip (230) and the track groove (220).

10. A miniature vacuum device for synchrotron infrared spectroscopy in biological sample experiments according to claim 6, characterized in that, When the transmission mechanism (400) is in operation, the slide rods (510) on both sides move in opposite directions.

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