Chinese grass turtle egg protein peptide extract with anti-oxidation effect as well as preparation method and application of Chinese grass turtle egg protein peptide extract

Chinese pond turtle egg protein peptides were prepared by ultrasound-assisted papain method and Sephadex G-25 dextran gel column chromatography, which solved the problem of unclear composition and structure of Chinese pond turtle egg protein peptides, and achieved efficient antioxidant function, which has broad application prospects.

CN121824680APending Publication Date: 2026-04-10ZHEJIANG UNIV OF TECH +1
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Patent Information

Application Number
CN202610065587.7
Authority / Receiving Office
CN · China
Patent Type
Applications(China)
Current Assignee / Owner
Filing Date
2026-01-19
Publication Date
2026-04-10

AI Technical Summary

Technical Problem

Existing research has focused little on protein peptides from Chinese pond turtle eggs, and there are no reports on the preparation of protein peptides from Chinese pond turtle eggs, clarifying their composition and structure, as well as their antioxidant activity, which limits their high-value utilization.

Method used

Chinese pond turtle egg protein peptides were prepared using an ultrasound-assisted papain method. Six peptide segments with high content were identified by LC-MS/MS. High-purity Chinese pond turtle egg protein peptide extracts were prepared by Sephadex G-25 dextran gel column chromatography, preserving their antioxidant activity.

Benefits of technology

The prepared Chinese pond turtle egg protein peptide extract has significant ABTS radical, DPPH radical, and hydroxyl radical scavenging ability and iron ion reducing ability, showing significant antioxidant effects and providing an antioxidant strategy.

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Abstract

The invention relates to an anti-oxidation Chinese grass turtle egg protein peptide extract as well as a preparation method and application thereof, and belongs to the technical field of biological preparation. The invention relates to a Chinese grass turtle egg protein peptide extract, which comprises peptide fragments with amino acid sequences as shown in SEQ ID NO.1-6. The preparation method comprises the steps of separation, enzymolysis, purification and the like, and specifically comprises the following steps: separating out egg white, freeze-drying, preparing egg white crude protein peptide hydrolysate by using an ultrasonic-assisted enzymolysis method, and finally purifying, freezing and drying to obtain protein peptide powder. The Chinese grass turtle egg protein peptide extract is prepared by adopting an ultrasonic-assisted papain method, the obtained polypeptide is relatively high in purity and clear in main component and structure, the activity is well maintained, and the Chinese grass turtle egg protein peptide extract has the capabilities of removing ABTS free radicals, DPPH free radicals and hydroxyl free radicals and enhancing iron ion reduction, and has a remarkable antioxidant effect.
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