Immature bitter orange extracellular vesicle-like particle as well as preparation method and application thereof

By preparing extracellular vesicle-like nanoparticles with an average particle size of 60-120 nm and a negatively charged surface from Citrus aurantium, the problem of separating and purifying Citrus aurantium vesicle-like particles was solved, and therapeutic and preventive effects were achieved on a variety of diseases, especially liver-related diseases and mental stress-related diseases.

CN121852306APending Publication Date: 2026-04-14GUANGDONG HOSPITAL OF TRADITIONAL CHINESE MEDICINE
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Patent Information

Application Number
CN202511868983.5
Authority / Receiving Office
CN · China
Patent Type
Applications(China)
Current Assignee / Owner
Filing Date
2025-12-11
Publication Date
2026-04-14

AI Technical Summary

Technical Problem

The existing technology has not yet systematically isolated and purified the vesicle-like particles of Citrus aurantium, and its bioactivity and application potential in various liver-related diseases and mental stress-related diseases have not been fully evaluated.

Method used

A method for preparing extracellular vesicle-like nanoparticles from Citrus aurantium is provided. Through the steps of crushing, filtering, centrifugation, and resuspension, extracellular vesicle-like nanoparticles derived from Citrus aurantium with an average particle size of 60-120 nm and a negatively charged surface are obtained. These nanoparticles encapsulate active substances such as naringin, neohesperidin, naringenin, and hesperidin and have liver-targeting properties.

Benefits of technology

The prepared Citrus aurantium vesicle-like nanoparticles are stable and liver-targeting, and can significantly treat diseases such as acute liver injury, non-alcoholic fatty liver, depression, and breast cancer mediated by chronic psychological stress, providing a new approach to treatment and prevention.

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Abstract

The invention belongs to the technical field of traditional Chinese medicines, and discloses an immature bitter orange-derived extracellular vesicle-like nanoparticle, which is characterized in that the immature bitter orange-derived extracellular vesicle-like nanoparticle is prepared by the steps of crushing immature bitter orange, filtering, centrifuging and resuspending, the average particle size is 60-120 nm, and the particle size of the immature bitter orange-derived extracellular vesicle-like nanoparticle is 20-30 nm. The extracellular vesicle-like nanoparticles are used for entrapment of at least one of naringin, neohesperidin, naringenin and hesperidin. The extracellular vesicle-like nanoparticles derived from immature bitter oranges are simple in preparation method, have liver targeting and good stability, and can be used for preparing medicines for preventing and / or treating liver injury, depression and breast cancer.
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