L-threonine aldolase mutant and application thereof in synthesis of L-serine

By directionally modifying L-threonine aldolase, a mutant containing specific amino acid mutation sites was constructed, solving the problems of insufficient catalytic activity, poor substrate tolerance, and low thermal stability. This enabled the efficient catalytic synthesis of L-serine from glycine and formaldehyde, making it suitable for industrial production.

CN121874166APending Publication Date: 2026-04-17BAODING JIUFU BIOCHEMICAL CO LTD +1
3 Cites 0 Cited by

Patent Information

Application Number
CN202512019448.9
Authority / Receiving Office
CN · China
Patent Type
Applications(China)
Current Assignee / Owner
Filing Date
2025-12-30
Publication Date
2026-04-17

AI Technical Summary

Technical Problem

Existing L-threonine aldolases have insufficient catalytic activity, poor tolerance to high-concentration substrates, and low thermal stability, making it difficult to meet the needs of large-scale industrial production.

Method used

By directionally modifying wild-type L-threonine aldolase, mutants containing specific amino acid mutation sites are constructed to improve catalytic activity, substrate tolerance, and thermal stability, thus forming L-threonine aldolase mutants or their active fragments.

Benefits of technology

It significantly improved the catalytic activity and thermal stability of L-threonine aldolase, enhanced its tolerance to high-concentration substrates, and increased the yield and production efficiency of L-serine, making it suitable for industrial applications.

✦ Generated by Eureka AI based on patent content.
Patent Text Reader

Abstract

The invention provides an L-threonine aldolase mutant and application thereof in synthesis of L-serine, the amino acid sequence of the L-threonine aldolase mutant comprises at least one mutation selected from the following mutations compared with a wild type amino acid sequence as shown in SEQ ID NO: 2: 1) serine S at the 8th site is mutated into arginine R or cysteine C; the L-threonine aldolase mutant provided by the invention has the advantages that the L-threonine aldolase mutant has a synergistic regulation effect through combination of different mutation sites, the capability of synthesizing L-serine by L-threonine aldolase is obviously improved, and the L-threonine aldolase mutant has better thermal stability and formaldehyde tolerance.
Need to check novelty before this filing date? Find Prior Art