Ribosomal protein n' mutant and application thereof

By editing the priA gene in Escherichia coli using gene editing technology, the N' mutant ribosomal protein PriA Y190D was obtained, which solved the problem of insufficient production of human milk oligosaccharides and significantly increased the yield of LNT II, ​​LNnT, LNT, 3'-SL and 6'-SL.

CN121874171BActive Publication Date: 2026-06-26TIANJIN UNIV OF SCI & TECH
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Patents(China)
Current Assignee / Owner
TIANJIN UNIV OF SCI & TECH
Filing Date
2026-03-17
Publication Date
2026-06-26

AI Technical Summary

Technical Problem

In existing technologies, the production of human milk oligosaccharides is insufficient, especially as the growth and production capacity of strains are limited in the later stages of fermentation.

Method used

By editing the priA gene in Escherichia coli using gene editing technology, the ribosomal protein N' mutant PriA Y190D was obtained, which increased the production of human milk oligosaccharides.

Benefits of technology

The N' mutant PriA Y190D significantly increased the production of LNT II, ​​LNnT, LNT, 3'-SL and 6'-SL by 28.6%, 50%, 92.3%, 13.8% and 20%, respectively.

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Abstract

The application belongs to the technical field of genetic engineering, and particularly relates to a ribosomal protein N' mutant and application thereof. The ribosomal protein N' mutant is subjected to Y190D mutation. When the ribosomal protein N' expressed by a production strain is subjected to Y190D mutation, the production of LNT II is increased by 28.6%, the production of LNnT is increased by 50%, the production of LNT is increased by 92.3%, the production of 3'-SL is increased by 13.8%, and the production of 6'-SL is increased by 20%. It can be seen that after the 190th amino acid of PriA is changed from tyrosine to aspartic acid, the production capacity is improved. When the ribosomal protein N' mutant is applied to an Escherichia coli for producing human milk oligosaccharides, the production of human milk oligosaccharides is significantly improved.
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