Application of algal NySAT2 gene in regulating crude protein and washing fiber of alfalfa
By introducing the NySAT2 gene from algae into alfalfa and optimizing it through genetic engineering, the problems of increasing crude protein and reducing detergent fiber were solved, achieving efficient breeding of alfalfa and improving its nutritional value and digestibility.
CN121874257BActive Publication Date: 2026-07-24SHANDONG UNIV +1
2 Cites 0 Cited by
Patent Information
- Application Number
- CN202610345457.9
- Authority / Receiving Office
- CN · China
- Patent Type
- Patents(China)
- Current Assignee / Owner
- Filing Date
- 2026-03-20
- Publication Date
- 2026-07-24
- Estimated Expiration
- 2046-03-20
AI Technical Summary
Technical Problem
Existing technologies make it difficult to further increase the crude protein content and reduce the content of indigestible detergent fiber without affecting the normal growth of alfalfa, so as to improve the nutritional value and digestibility of forage.
Method used
By introducing the NySAT2 gene from algae, the gene was overexpressed in alfalfa using genetic engineering techniques, including plasmid introduction, increasing gene copy number, altering promoter sequence, or linking strong promoters, to achieve efficient gene expression.
Benefits of technology
While maintaining stable growth vigor of alfalfa, it significantly increases crude protein content and reduces acid and neutral detergent fiber content, thereby improving the quality and feed value of forage.
✦ Generated by Eureka AI based on patent content.
Abstract
The present application relates to the field of plant genetic engineering technology, and particularly relates to the application of algal NySAT2 gene in regulating alfalfa crude protein and washing fiber. The present application uses gene synthesis and codon optimization technology to obtain the sequence of the coding region of the codon-optimized NySAT2 gene, constructs an algal NySAT2 gene overexpression vector, introduces the target gene into the alfalfa genome through the Agrobacterium rhizogenes-mediated genetic transformation method, and obtains the transgenic plant using the genetic transformation system. Through molecular identification and phenotype analysis, it is found that the alfalfa plants overexpressing the NySAT2 gene have a significantly increased crude protein content compared with the wild-type alfalfa plants, while the contents of acid detergent fiber and neutral detergent fiber are significantly reduced, effectively improving the forage quality and feeding value of alfalfa.
Need to check novelty before this filing date? Find Prior Art