Molecular identification chip for permanent resistance of rice blast and application of molecular identification chip

By using molecular identification chips and CRI models, the problems of easy failure of resistance and low identification efficiency in rice blast resistance breeding have been solved. This has enabled durable and broad-spectrum resistance assessment, reduced chemical control, improved breeding efficiency and genetic diversity, and is applicable to a variety of crops and diseases.

CN121874384APending Publication Date: 2026-04-17NORTHEAST AGRICULTURAL UNIVERSITY
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Patent Information

Application Number
CN202610032149.0
Authority / Receiving Office
CN · China
Patent Type
Applications(China)
Current Assignee / Owner
Filing Date
2026-01-12
Publication Date
2026-04-17

AI Technical Summary

Technical Problem

In existing technologies, rice blast resistance breeding relies on a single major resistance R gene, which makes resistance easily overcome by pathogens. Traditional phenotypic identification is inefficient, cannot achieve durable and broad-spectrum resistance, has a high dependence on chemical control, lacks sufficient intelligence in breeding, and is limited in cross-crop application.

Method used

Develop a molecular identification chip containing 600 to 800 specific nucleic acid probes, covering functional genetic loci validated by multi-year, multi-location, and multi-race inoculation experiments and genome-wide association analysis. Combine machine learning algorithms to construct a comprehensive resistance index (CRI) model to achieve high-throughput, quantitative assessment of the durability resistance of rice materials.

Benefits of technology

It significantly improves the broad-spectrum and stability of rice against rice blast, reduces the use of chemical fungicides, shortens the breeding cycle, broadens the genetic base, supports the synergistic improvement of multiple traits, and is applicable to a variety of rice diseases and other crops, promoting green plant protection and ecological agriculture.

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Abstract

The invention provides a molecular identification chip aiming at permanent resistance of rice blast and application thereof, the chip comprises 600-800 specific nucleic acid probes fixed on a solid phase carrier, each probe corresponds to a functional genetic locus subjected to multi-year, multi-point and multi-race inoculation tests and whole genome association analysis verification, and the molecular identification chip is used for identifying the permanent resistance of the rice blast. According to the method, a comprehensive resistance index (CRI) prediction model is constructed by combining high-throughput typing with machine learning. During breeding, the lasting resistance level of the material to multiple rice blast can be rapidly quantified in the seedling stage, and early-stage accurate screening is achieved. Compared with a traditional method depending on a single major R gene, the method has the advantages that the resistance broad spectrum and durability are remarkably improved, the field identification cost is reduced by 70% or above, and pesticide application reduction and green production are supported. The technology provides an efficient, intelligent and sustainable molecular tool for rice disease-resistant breeding.
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