Device for removing background fluorescence of tissue sample

Abstract

The invention relates to a device for removing background fluorescence of a tissue sample, the device comprises a first shell, a light source module, a sample module, a cooling module and a control module, the light source module and the cooling module are both mounted in an inner cavity of the first shell; the sample module can mix a tissue sample with a reducing agent and then fix the mixture in an inner cavity of the first shell, and the reducing agent comprises a metal hydride reducing agent; the light source module can emit a light source for irradiating the sample module, and the light source and the reducing agent act on the tissue sample to remove background fluorescence of the tissue sample; the cooling module is used for cooling the inner cavity of the first shell; the control module is connected with the cooling module and the light source module. According to the scheme, background fluorescence of a tissue sample can be obviously removed, the background is cleaner after the treated tissue is subjected to antibody staining, and the signal fidelity is improved; the removal effect is thorough, background fluorescence cannot be recovered, and the stability is high; the method basically has no influence on tissue morphology, is basically suitable for all tissues of animals and plants, is not limited by types, and is high in universality.