Signal processing method for cell weak-force electric signal detection

Abstract

The invention provides a signal processing method for cell weak-force electric signal detection, a cell signal detection system and detection equipment are included, a culture dish in the system is used for bearing a culture solution, the culture solution contains a plurality of cells, and an opening of the culture dish is provided with a first electrode and a second electrode which are connected to the culture solution; the first end of the control module is coupled to the first electrode and used for sending an excitation signal to the first electrode based on the configuration information; the first end of the signal acquisition module is in negative pressure contact with any cell and acquires a first signal of the cell; the signal processing module is used for acquiring a first signal corresponding to the excitation signal after the excitation signal is sent out; after first signals corresponding to the excitation signals are obtained, all the obtained first signals are averaged, and accurate electric signals and motion signals are obtained; and the weak signal analysis module is used for performing weak signal analysis on the motion signal and the electric signal. Therefore, accurate movement signals and electric signals of the cells can be detected outside the cells.

Description

A signal processing method for detecting weak electromechanical signals in cells Technical Field

[0001] This invention relates to the field of cell signal detection, and more particularly to a signal processing method for detecting weak electrochemical signals in cells. Background Technology

[0002] Cellular motility and electrical signals are crucial manifestations of cellular physiological activity, playing a vital role in health monitoring, disease diagnosis, and drug screening. Taking cardiomyocytes as an example, precise analysis of their motility and electrical signals enables effective monitoring, accurate diagnosis, and efficient drug screening for cardiac diseases such as arrhythmias and myocardial infarction.

[0003] However, since cellular motion and electrical signals are very weak and easily affected by external noise, it is very difficult to detect motion and electrical signals outside the cell in the current technology, and it is also difficult to detect accurate motion and electrical signals.

[0004] Therefore, how to accurately detect the motion and electrical signals of cells outside the cell has become a pressing technical problem that needs to be solved by existing technologies. Summary of the Invention

[0005] This invention provides a cell signal detection system and detection device, which solves the technical problem of detecting accurate motion signals and electrical signals of cells outside the cell.

[0006] According to a first aspect of the present invention, the present invention provides a cell weak electromotive force signal detection system, comprising: a culture dish for holding a culture medium containing a plurality of cells, wherein the opening of the culture dish has a first electrode and a second electrode connected to the culture medium;

[0007] A control module, the first end of which is coupled to the first electrode, is used to send an excitation signal to the first electrode based on configuration information;

[0008] The signal acquisition module has its first end in negative pressure contact with any cell.

[0009] The signal acquisition module is used to acquire a first signal of the cell, the first signal including a first motion signal and a first electrical signal;

[0010] A signal processing module has a first input terminal coupled to a second terminal of the control module and a second input terminal coupled to a second terminal of the signal acquisition module; the signal processing module is used to acquire the first signal corresponding to the excitation signal when the excitation signal is received; and

[0011] After acquiring the first signal corresponding to the excitation signal, all the acquired first signals are averaged to obtain an electrical signal and a motion signal, wherein the motion signal is the average signal of all the first motion signals of the cell, and the electrical signal is the average signal of all the first electrical signals of the cell.

[0012] A weak signal analysis module is coupled to the output of the signal processing module to receive the first motion signal and the first electrical signal.

[0013] The weak signal analysis module is used to perform weak signal analysis on the motion signal and the electrical signal respectively.

[0014] Optionally, the signal acquisition module includes a motion signal acquisition submodule and an electrical signal acquisition submodule;

[0015] The motion signal acquisition submodule forms a negative pressure contact with the cell, the second end of the motion signal acquisition submodule is coupled to the second input end of the signal processing module, and the motion signal acquisition submodule is located on one side of the opening of the culture dish;

[0016] The first end of the electrical signal acquisition submodule is coupled to the cell, and the second end of the electrical signal acquisition submodule is coupled to the third input end of the signal processing module. The electrical signal acquisition submodule is used to acquire and transmit the first electrical signal.

[0017] Optionally, the motion signal acquisition submodule includes a first probe and a deformation detection unit;

[0018] The first probe is in negative pressure contact with the cells, and the first probe is located on one side of the opening of the culture dish;

[0019] The first end of the deformation detection unit is coupled to the second input end of the signal processing module. The deformation detection unit is used to acquire and amplify the deformation of the first probe, and acquire the first motion signal of the cell based on the amplified deformation of the first probe.

[0020] Optionally, the first probe is a hollow microtubule probe containing a solution. The microtubule probe includes a first microtubule segment and a second microtubule segment. The first end of the first microtubule segment is in negative pressure contact with the cell, and the second end of the first microtubule segment is connected to the second microtubule segment. The solution is located in the first microtubule segment, and the first microtubule segment and the second microtubule segment have a first angle.

[0021] The deformation detection unit includes a reflective microsphere and a laser interferometer detector. The reflective microsphere is connected to the outside of the microtube probe and is located at the connection between the first microtube segment and the second microtube segment of the microtube probe.

[0022] The laser interferometer is used to emit a laser beam toward the reflective microsphere, receive the reflected beam of the laser beam, and convert the reflected beam into the first motion signal.

[0023] Optionally, the electrical signal acquisition submodule includes a second probe, a laser emitting unit, and a position detection unit;

[0024] The second probe forms a negative pressure contact with the cell, and the laser emitting unit, the position detection unit, and the second probe are all located on one side of the opening of the culture dish;

[0025] The laser emitting unit is used to emit a laser beam toward the second probe;

[0026] The input terminal of the position detection unit receives the reflected beam of the laser beam, and the output terminal of the position detection unit is coupled to the second input terminal of the signal processing unit. The position detection unit is used to convert the reflected beam into the first motion signal.

[0027] Optionally, the electrical signal acquisition submodule includes a third probe and a first wire. The third probe is a hollow microtube probe, and the microtube of the third probe is filled with a conductive solution.

[0028] The first end of the first wire is coupled to the conductive solution, and the second end of the first wire is coupled to the third input terminal of the signal processing module.

[0029] Optionally, the signal processing module includes: a motion signal storage unit, a motion signal denoising unit, an electrical signal storage unit, and an electrical signal denoising unit;

[0030] The first end of the motion signal storage unit is coupled to the second end of the motion signal acquisition submodule, the second end of the motion signal storage unit is coupled to the first end of the motion signal denoising unit, the third end of the motion signal storage unit is coupled to the second end of the control module, and the second end of the motion signal denoising unit is coupled to the first end of the weak signal processing module.

[0031] The motion signal storage unit is used to acquire and store the first motion signal of the cell each time after receiving the excitation signal, and send all the stored first motion signals to the motion signal denoising unit.

[0032] The motion signal denoising unit is used to average all the acquired first motion signals to obtain a motion signal;

[0033] The first end of the electrical signal storage unit is coupled to the second end of the electrical signal acquisition submodule, the second end of the electrical signal storage unit is coupled to the first end of the electrical signal denoising unit, the third end of the electrical signal storage unit is coupled to the second end of the control module, and the second end of the electrical signal denoising unit is coupled to the second end of the weak signal processing module.

[0034] The electrical signal storage unit is used to acquire and store the first electrical signal of the cell each time after receiving the excitation signal, and send all the stored first electrical signals to the electrical signal denoising unit.

[0035] The electrical signal denoising unit is used to average all the acquired first electrical signals to obtain an electrical signal.

[0036] Optionally, the control module further includes a drive signal submodule and an excitation submodule;

[0037] The first end of the drive signal submodule is coupled to the first end of the excitation submodule, the second end of the excitation submodule is coupled to the first electrode, and the third end of the excitation submodule is coupled to the second input end of the signal processing module.

[0038] The drive signal submodule is used to generate drive signals based on the configuration information. The drive signals include pulse quantity information and pulse waveform information contained in each excitation signal.

[0039] The excitation submodule is used to send the corresponding excitation signal to the first electrode based on the driving signal.

[0040] Optionally, the cell signal detection system includes a moving module;

[0041] The moving module is coupled to the culture dish and is used to move the culture dish to control the signal acquisition module to acquire signals from different cells.

[0042] According to a second aspect of the present invention, the present invention provides a detection device comprising the cell weak electrical signal detection system described in any of the preceding claims.

[0043] A signal processing method for detecting weak electrochemical signals in cells, applied to a weak electrochemical signal detection system, includes the following steps:

[0044] A culture dish holds a culture medium containing a plurality of cells, and the opening of the culture dish has a first electrode and a second electrode connected to the culture medium;

[0045] A control module, the first end of which is coupled to the first electrode, the control module sends an excitation signal to the first electrode based on configuration information;

[0046] The signal acquisition module has its first end in negative pressure contact with any cell.

[0047] The signal acquisition module acquires a first signal from the cell, the first signal including a first motion signal and a first electrical signal;

[0048] A signal processing module has its first input terminal coupled to the second terminal of the control module, and its second input terminal coupled to the second terminal of the signal acquisition module; when the signal processing module receives the excitation signal, it acquires the first signal corresponding to the excitation signal; and

[0049] After acquiring the first signal corresponding to the excitation signal, all the acquired first signals are averaged to obtain an electrical signal and a motion signal, wherein the motion signal is the average signal of all the first motion signals of the cell, and the electrical signal is the average signal of all the first electrical signals of the cell.

[0050] A weak signal analysis module is coupled to the output of the signal processing module to receive the first motion signal and the first electrical signal.

[0051] The weak signal analysis module performs weak signal analysis on the motion signal and the electrical signal respectively.

[0052] Compared with the prior art, the technical solution of the embodiments of the present invention has the following beneficial effects:

[0053] This invention provides a system, device, and signal processing method for detecting weak electrochemical signals in cells. The system includes a culture dish containing a plurality of cells, with a first electrode and a second electrode connected to the culture medium at the opening of the dish. A control module is coupled to the first electrode and sends an excitation signal to the first electrode based on configuration information. A signal acquisition module forms a negative pressure contact with any cell and acquires the cell's first signal. A signal processing module acquires the first signal corresponding to the excitation signal after it is emitted, and averages all acquired first signals to obtain precise electrical and motion signals. A weak signal analysis module performs weak signal analysis on the motion and electrical signals respectively. Thus, this invention enables the precise detection of cell motion and electrical signals outside the cell. Attached Figure Description

[0054] To more clearly illustrate the technical solutions in the embodiments of the present invention or the prior art, the drawings used in the description of the embodiments or the prior art will be briefly introduced below. Obviously, the drawings described below are only some embodiments of the present invention. For those skilled in the art, other drawings can be obtained based on these drawings without creative effort.

[0055] Figure 1 is a schematic diagram of the structure of a first type of weak electrical signal detection system for cells provided in an embodiment of the present invention;

[0056] Figure 2 is a schematic diagram of the structure of a second type of weak electrical signal detection system for cells provided in an embodiment of the present invention;

[0057] Figure 3 is a schematic diagram of the structure of a third type of weak electrical signal detection system for cells provided in an embodiment of the present invention;

[0058] Figure 4 is a schematic diagram of the structure of a fourth type of weak electrical signal detection system for cells provided in an embodiment of the present invention;

[0059] Figure 5 is a schematic diagram of the structure of a fifth type of weak electrical signal detection system for cells provided in an embodiment of the present invention;

[0060] Figure 6 is a schematic diagram of a signal detection module according to an embodiment of the present invention;

[0061] Figure 7 is a schematic diagram of the structure of a control module provided in an embodiment of the present invention;

[0062] Figure 8 is a schematic diagram of the structure of a mobile module provided in an embodiment of the present invention. Detailed Implementation

[0063] The technical solutions of the embodiments of the present invention will be clearly and completely described below with reference to the accompanying drawings. Obviously, the described embodiments are only some embodiments of the present invention, and not all embodiments. Based on the embodiments of the present invention, all other embodiments obtained by those of ordinary skill in the art without creative effort are within the scope of protection of the present invention.

[0064] The terms “first,” “second,” “third,” “fourth,” etc. (if present) in the specification, claims, and accompanying drawings of this invention are used to distinguish similar objects and are not necessarily used to describe a particular order or sequence. It should be understood that such data can be interchanged where appropriate so that embodiments of the invention described herein can be implemented in orders other than those illustrated or described herein. Furthermore, the terms “comprising” and “having,” and any variations thereof, are intended to cover a non-exclusive inclusion; for example, a process, method, system, product, or apparatus that comprises a series of steps or units is not necessarily limited to those steps or units explicitly listed, but may include other steps or units not explicitly listed or inherent to such processes, methods, products, or apparatus.

[0065] The technical solution of the present invention will be described in detail below with reference to specific embodiments. These specific embodiments can be combined with each other, and the same or similar concepts or processes may not be described again in some embodiments.

[0066] As described in the background section, this invention aims to solve the technical problem of detecting precise motion and electrical signals of cells outside the cell. The following will provide a detailed description in conjunction with existing technologies.

[0067] Prior to filing this application, the applicant conducted extensive research on techniques for detecting cell signals.

[0068] One existing cell detection method uses patch-clamp technology to detect the electrical signals of cells. Specifically, this technique involves making a small opening in the cell membrane and inserting a probe into the cell to obtain a high signal-to-noise ratio electrical signal. However, this method not only damages the cells but also contaminates the probes, limiting the detection capability to one probe per cell.

[0069] Another existing method for cell detection is to detect the electrical signals of cells using microelectrode array technology. Specifically, this technique involves placing the first electrode in contact with the cell membrane. This method can acquire the electrical signals of cells without piercing the cell membrane. However, the electrical signals acquired in this way have relatively high noise, and therefore, the acquired electrical signals are not accurate enough.

[0070] It is evident that existing technologies can only obtain electrical signals, and have difficulty obtaining motion signals.

[0071] To address the aforementioned problems, this invention provides a cell weak electrophysiological signal detection system. The system includes a culture dish containing a culture medium with several cells. The opening of the culture dish has a first electrode and a second electrode connected to the culture medium. A control module is coupled to the first electrode and sends an excitation signal to the first electrode based on configuration information. A signal acquisition module forms a negative pressure contact with any cell and acquires the cell's first signal. A signal processing module acquires the first signal corresponding to the excitation signal after it is emitted. After acquiring the first signal, all acquired first signals are averaged to obtain accurate electrical and motion signals. A weak signal analysis module performs weak signal analysis on the motion and electrical signals respectively. Thus, this invention achieves accurate detection of cell motion and electrical signals outside the cell.

[0072] To make the above-mentioned objectives, features and beneficial effects of the present invention more apparent and understandable, specific embodiments of the present invention will be described in detail below with reference to the accompanying drawings.

[0073] Please refer to Figure 1. An embodiment of the present invention provides a cellular weak electromechanical signal detection system, comprising:

[0074] A culture dish 100 is used to hold a culture medium 110, the culture medium 110 containing a plurality of cells 120, and the opening of the culture dish 100 has a first electrode 130 and a second electrode 140 connected to the culture medium 110.

[0075] A control module 200, the first end of which is coupled to the first electrode 130, is used to send an excitation signal to the first electrode 130 based on configuration information.

[0076] The first end of the signal acquisition module 300 forms a negative pressure contact with any one of the cells 120;

[0077] The signal acquisition module 300 is used to acquire a first signal of the cell 120, the first signal including a first motion signal and a first electrical signal;

[0078] A signal processing module 400, with its first terminal coupled to the second terminal of the control module 200 and its second terminal coupled to the second terminal of the signal acquisition module 300; the signal processing module 400 is used to acquire the first signal corresponding to the excitation signal when the excitation signal is received; and

[0079] After acquiring the first signal corresponding to the excitation signal, all the acquired first signals are averaged to obtain an electrical signal and a motion signal, wherein the electrical signal is the average signal of all the first electrical signals of the cell 120, and the motion signal is the average signal of all the first motion signals of the cell 120.

[0080] The weak signal analysis module 500 is coupled to the output terminal of the signal processing module 400 and receives the first motion signal and the first electrical signal.

[0081] The weak signal analysis module 500 is used to perform weak signal analysis on the motion signal and the electrical signal respectively.

[0082] A signal processing method for detecting weak electrochemical signals in cells, applied to a weak electrochemical signal detection system, includes the following steps:

[0083] A culture dish 100 holds a culture medium containing a plurality of cells;

[0084] The control module 200 sends an excitation signal to the first electrode 130 based on the configuration information;

[0085] The signal acquisition module 300 acquires a first signal of the cell, the first signal including a first motion signal and a first electrical signal;

[0086] When the signal processing module 400 receives the excitation signal, it acquires the first signal corresponding to the excitation signal; and after acquiring the first signal corresponding to the excitation signal, it performs average processing on all the acquired first signals to obtain an electrical signal and a motion signal, wherein the motion signal is the average signal of all the first motion signals of the cell, and the electrical signal is the average signal of all the first electrical signals of the cell.

[0087] The weak signal analysis module 500 receives and analyzes the first motion signal and the first electrical signal.

[0088] As can be seen, this system sends an excitation signal to the cell through the control module. After sending the excitation signal, the signal acquisition module makes negative pressure contact with the cell, thereby acquiring the cell's first motion signal and first electrical signal without damaging the cell. Then, the signal processing module, upon receiving the first motion signal and first electrical signal, averages all of them to obtain the electrical signal and motion signal respectively, thus removing noise from the first motion signal and first electrical signal. This allows the weak signal processing module to obtain the cell's precise motion signal and electrical signal. This achieves the precise detection of cell motion and electrical signals outside the cell.

[0089] As a preferred embodiment, please refer to FIG2, the signal acquisition module 300 includes a motion signal acquisition submodule 310 and an electrical signal acquisition submodule 320;

[0090] The first end of the motion signal acquisition submodule 310 forms a negative pressure contact with the cell 120, the second end of the motion signal acquisition submodule 310 is coupled to the second input end of the signal processing module 400, and the motion signal acquisition submodule 310 is located on one side of the opening of the culture dish 100.

[0091] The first end of the electrical signal acquisition submodule 320 is coupled to the first probe 310, and the second end of the electrical signal acquisition submodule 320 is coupled to the third input end of the signal processing module 400. The electrical signal acquisition submodule 320 is used to acquire and transmit the first electrical signal.

[0092] As one implementation, please refer to Figure 3. The motion signal acquisition submodule 310 includes a deformation detection unit 311 and a first probe 312.

[0093] The first probe 312 is in negative pressure contact with the cells 120, and the first probe 312 is located on one side of the opening of the culture dish 100;

[0094] The first end of the deformation detection unit 311 is coupled to the second input end of the signal processing module 400. The deformation detection unit 311 is used to acquire and amplify the deformation of the first probe 312, and acquire the first motion signal of the cell 120 based on the amplified deformation of the first probe 312.

[0095] In a specific implementation, the position of the first probe 312 remains unchanged when detecting the motion signal of the same cell.

[0096] In the process of acquiring the first motion signal and the first electrical signal of the cell 120, the present invention only requires the first probe 312 to be in negative pressure contact with the cell 120. The first probe 312 does not damage the cell membrane, so the cell is not damaged and the first probe is not contaminated. The first probe can be reused, saving costs.

[0097] As an example, as shown in Figure 3, the first probe 312 can be coupled to the signal processing module 400. It should be understood that the first probe 312 can also be coupled to the control module 200 or a displacement module to control the movement or fixation of the first probe 312; this invention does not impose specific limitations here.

[0098] As a specific implementation, please continue to refer to Figure 3. The first probe 312 shown is a hollow microtubule probe containing a solution 3121. The microtubule probe includes a first microtubule segment 3122 and a second microtubule segment 3123. The first end of the first microtubule segment 3122 is in negative pressure contact with the cell 120, and the second end of the first microtubule segment 3122 is connected to the second microtubule segment 3123. The solution 3121 is located in the first microtubule segment 3122, and the first microtubule segment 3122 and the second microtubule segment 3123 have a first included angle.

[0099] The deformation detection unit 311 includes a reflective microsphere 3111 and a laser interferometer 3112. The reflective microsphere 3111 is connected to the outside of the microtube probe and is located at the connection between the first microtube segment 3122 and the second microtube segment 3123 of the microtube probe.

[0100] The laser interferometer 3112 is used to emit a laser beam to the reflective microsphere 3111, receive the reflected beam of the laser beam, and convert the reflected beam into the first motion signal.

[0101] As another implementation, please refer to FIG4. The motion signal acquisition submodule 310 may also include a second probe 313, a laser emitting unit 314 and a position detection unit 315.

[0102] The first end of the second probe 313 is in negative pressure contact with the cell, and the laser emitting unit 314, the position detection unit 315 and the second probe 313 are all located on one side of the opening of the culture dish 100;

[0103] The laser emitting unit 314 is used to emit a laser beam toward the second probe 313;

[0104] The input terminal of the position detection unit 315 receives the reflected beam of the laser beam, and the output terminal of the position detection unit 315 is coupled to the second input terminal of the signal processing module 400. The position detection unit 315 is used to convert the laser beam into the first motion signal.

[0105] As shown in Figure 4, the laser emitting unit 314 only needs to be able to emit a laser beam to the position detection unit, so that the position detection unit 315 receives the reflected beam of the laser beam after passing through the first probe 313. The laser emitting unit can be connected to any module in this invention, or it can be not connected to any module in this invention; this invention is not limited thereto.

[0106] The position probe unit 315 is a photodiode spot detector (PSD).

[0107] As a specific embodiment, please refer to Figure 5. The electrical signal acquisition submodule 320 includes a third probe 321 and a first wire 322. The third probe 321 is a hollow microtube probe, and the microtube of the third probe 321 is filled with a conductive solution 3211.

[0108] The first end of the first wire 322 is coupled to the conductive solution 3211, and the second end of the first wire 322 is coupled to the third input terminal of the signal processing module 400.

[0109] In the process of acquiring the first motion signal and the first electrical signal of the cell 120, the present invention only requires the third probe 321 to come into negative pressure contact with the cell 120. The third probe 321 does not damage the cell membrane, so the cell is not damaged and the first probe is not contaminated. The third probe 321 can be reused, saving costs.

[0110] As one specific implementation, please refer to Figure 6. The signal processing module 400 includes: a motion signal storage unit 411, a motion signal denoising unit 412, an electrical signal storage unit 421, and an electrical signal denoising unit 422.

[0111] The first end of the motion signal storage unit 411 is coupled to the second end of the motion signal acquisition submodule 310, the second end of the motion signal storage unit 411 is coupled to the first end of the motion signal denoising unit 412, the third end of the motion signal storage unit 411 is coupled to the second end of the control module 200, and the second end of the motion signal denoising unit 412 is coupled to the first end of the weak signal processing module 500.

[0112] The motion signal storage unit 411 is used to acquire and store the first motion signal of the cell 120 each time after receiving the excitation signal, and send all the stored first motion signals to the motion signal denoising unit 412.

[0113] The motion signal denoising unit 412 is used to average all the acquired first motion signals to obtain a motion signal;

[0114] The first end of the electrical signal storage unit 421 is coupled to the second end of the electrical signal acquisition submodule 320, the second end of the electrical signal storage unit 421 is coupled to the first end of the electrical signal noise reduction unit 422, the third end of the electrical signal storage unit 421 is coupled to the second end of the control module 200, and the second end of the electrical signal noise reduction unit 422 is coupled to the second end of the weak signal processing module 500.

[0115] The electrical signal storage unit 421 is used to acquire and store the first electrical signal of the cell 120 each time after receiving the excitation signal, and send all the stored first electrical signals to the electrical signal denoising unit 422.

[0116] The electrical signal denoising unit 422 is used to average all the acquired first electrical signals to obtain an electrical signal.

[0117] As one implementation, please refer to FIG7, the control module 200 further includes a drive signal submodule 210 and an excitation submodule 220;

[0118] The first end of the drive signal submodule 210 is coupled to the first end of the excitation submodule 220, the second end of the excitation submodule 220 is coupled to the first electrode 130, and the third end of the excitation submodule 220 is coupled to the second input end of the signal processing module 400.

[0119] The drive signal submodule 210 is used to generate a drive signal based on the configuration information. The drive signal includes information on the number of pulses contained in each excitation signal and information on the pulse waveform.

[0120] The excitation submodule 220 is used to send the corresponding excitation signal to the first electrode 130 based on the driving signal.

[0121] As one implementation, please refer to Figure 8, the cell signal detection system further includes a moving module 600;

[0122] The moving module 600 is coupled to the culture dish 100 and is used to move the culture dish 100 to control the signal acquisition module 300 to acquire signals from different cells 120.

[0123] However, this invention is not limited to this. As another embodiment, the mobile device 600 can also be coupled to the signal acquisition module 300. By controlling the movement of the signal acquisition module 300 when acquiring different cells, the signal acquisition module 300 can acquire signals from different cells 120. Further details of this invention will not be elaborated upon here.

[0124] In summary, this invention provides a weak electrical signal detection system and device for cells. The system sends an excitation signal to the cell via a control module. After sending the excitation signal, a signal acquisition module makes negative pressure contact with the cell, acquiring the cell's first motion signal and first electrical signal without damaging the cell. Then, a cell detection module receives the first motion signal and first electrical signal, and averages all of them to obtain the electrical signal and motion signal respectively, removing noise from both signals. This allows the weak signal processing module to obtain accurate motion and electrical signals from the cell. This achieves accurate detection of cell motion and electrical signals outside the cell.

[0125] In addition, the present invention also provides a detection device, including the cell signal detection system described in any of the above claims.

[0126] As an example, this detection device can be used as equipment in drug development. Of course, it should be understood that the present invention is not limited thereto, and the detection device can also be used as other types of equipment that require a cell signal detection system.

[0127] While the present invention has been disclosed above, it is not limited thereto. Any person skilled in the art can make various modifications and alterations without departing from the spirit and scope of the invention; therefore, the scope of protection of the present invention should be determined by the scope defined in the claims.

Claims

1. A cellular weak electrophysiological signal detection system, characterized in that, include: A culture dish for holding a culture medium containing a plurality of cells, wherein the opening of the culture dish has a first electrode and a second electrode connected to the culture medium; A control module, the first end of which is coupled to the first electrode, is used to send an excitation signal to the first electrode based on configuration information; The signal acquisition module has its first end in negative pressure contact with any cell. The signal acquisition module is used to acquire a first signal of the cell, the first signal including a first motion signal and a first electrical signal; the signal processing module has a first input terminal coupled to a second terminal of the control module, and a second input terminal coupled to a second terminal of the signal acquisition module. The signal processing module is used to obtain the first signal corresponding to the excitation signal when the excitation signal is received; And after acquiring the first signal corresponding to the excitation signal, the first signals are averaged to obtain an electrical signal and a motion signal, wherein the motion signal is the average of all first motion signals of the cell, and the electrical signal is the average of all first electrical signals of the cell; a weak signal analysis module is coupled to the output of the signal processing module to receive the first motion signal and the first electrical signal. The weak signal analysis module is used to perform weak signal analysis on the motion signal and the electrical signal respectively.

2. The cell weak electrophysiological signal detection system as described in claim 1, characterized in that, The signal acquisition module includes a motion signal acquisition submodule and an electrical signal acquisition submodule; the first end of the motion signal acquisition submodule forms a negative pressure contact with the cell, the second end of the motion signal acquisition submodule is coupled to the second input end of the signal processing module, and the motion signal acquisition submodule is located on one side of the opening of the culture dish; The first end of the electrical signal acquisition submodule is coupled to the cell, and the second end of the electrical signal acquisition submodule is coupled to the third input end of the signal processing module. The electrical signal acquisition submodule is used to acquire and transmit the first electrical signal.

3. The cell weak electrophysiological signal detection system as described in claim 1, characterized in that, The motion signal acquisition submodule includes a first probe and a deformation detection unit; the first probe is in negative pressure contact with the cell and is located on one side of the opening of the culture dish; the first end of the deformation detection unit is coupled to the second input end of the signal processing module, and the deformation detection unit is used to acquire and amplify the deformation of the first probe, and acquire the first motion signal of the cell based on the amplified deformation of the first probe.

4. The cell weak electrophysiological signal detection system as described in claim 3, characterized in that, The first probe shown is a hollow microtubule probe containing a solution. The microtubule probe includes a first microtubule segment and a second microtubule segment. The first end of the first microtubule segment is in negative pressure contact with the cell, and the second end of the first microtubule segment is connected to the second microtubule segment. The solution is located in the first microtubule segment, and the first microtubule segment and the second microtubule segment have a first angle. The deformation detection unit includes a reflective microsphere and a laser interferometer. The reflective microsphere is connected to the outside of the microtubule probe and is located at the connection between the first microtubule segment and the second microtubule segment of the microtubule probe. The laser interferometer is used to emit a laser beam to the reflective microsphere, receive the reflected beam of the laser beam, and convert the reflected beam into the first motion signal.

5. The cellular weak electromechanical signal detection system as described in claim 2, characterized in that, The electrical signal acquisition submodule includes a second probe, a laser emitting unit, and a position detection unit; the second probe forms a negative pressure contact with the cell, and the laser emitting unit, the position detection unit, and the second probe are all located on one side of the opening of the culture dish; the laser emitting unit is used to emit a laser beam towards the second probe beam; the input end of the position detection unit receives the reflected beam of the laser beam, and the output end of the position detection unit is coupled to the second input end of the signal processing unit; the position detection unit is used to convert the reflected beam into the first motion signal.

6. The cell weak electromechanical signal detection system as described in claim 2, characterized in that, The electrical signal acquisition submodule includes a third probe and a first wire. The third probe is a hollow microtube probe, and the microtube of the third probe is filled with a conductive solution. The first end of the first wire is coupled to the conductive solution, and the second end of the first wire is coupled to the third input terminal of the signal processing module.

7. The cellular weak electrophysiological signal detection system as described in claim 2, characterized in that, The signal processing module includes: a motion signal storage unit, a motion signal denoising unit, an electrical signal storage unit, and an electrical signal denoising unit; a first end of the motion signal storage unit is coupled to a second end of the motion signal acquisition submodule, a second end of the motion signal storage unit is coupled to a first end of the motion signal denoising unit, a third end of the motion signal storage unit is coupled to a second end of the control module, and a second end of the motion signal denoising unit is coupled to a first end of the weak signal processing module; the motion signal storage unit is used to acquire and store the first motion signal of the cell each time after receiving the excitation signal, and send all the stored first motion signals to the motion signal denoising unit; the motion signal denoising unit... The unit is used to average all the acquired first motion signals to obtain a motion signal; the first end of the electrical signal storage unit is coupled to the second end of the electrical signal acquisition submodule, the second end of the electrical signal storage unit is coupled to the first end of the electrical signal denoising unit, the third end of the electrical signal storage unit is coupled to the second end of the control module, and the second end of the electrical signal denoising unit is coupled to the second end of the weak signal processing module; the electrical signal storage unit is used to acquire and store the first electrical signal of the cell each time after receiving the excitation signal, and send all the stored first electrical signals to the electrical signal denoising unit; the electrical signal denoising unit is used to average all the acquired first electrical signals to obtain an electrical signal.

8. The cellular weak electrophysiological signal detection system as described in claim 1, characterized in that, The control module further includes a drive signal submodule and an excitation submodule; the first end of the drive signal submodule is coupled to the first end of the excitation submodule, the second end of the excitation submodule is coupled to the first electrode, and the third end of the excitation submodule is coupled to the second input end of the signal processing module. The drive signal submodule is used to generate drive signals based on the configuration information. The drive signals include pulse quantity information and pulse waveform information contained in each excitation signal. The excitation submodule is used to send the corresponding excitation signal to the first electrode based on the driving signal.

9. The cellular weak electrophysiological signal detection system as described in claim 1, characterized in that, The cell signal detection system includes a moving module; the moving module is coupled to the culture dish and is used to move the culture dish to control the signal acquisition module to acquire signals from different cells.

10. A testing device, characterized in that, The system includes the cell weak electrical signal detection system according to any one of claims 1 to 9.

11. A signal processing method for detecting weak electrical signals in cells, applied to the weak electrical signal detection system of cells according to claim 1, comprising the following steps: A culture dish holds a culture medium containing a plurality of cells, and the opening of the culture dish has a first electrode and a second electrode connected to the culture medium; A control module, the first end of which is coupled to the first electrode, the control module sends an excitation signal to the first electrode based on configuration information; The signal acquisition module has its first end in negative pressure contact with any cell. The signal acquisition module acquires a first signal from the cell, the first signal including a first motion signal and a first electrical signal; the signal processing module has a first input terminal coupled to a second terminal of the control module, and a second input terminal coupled to a second terminal of the signal acquisition module; When the signal processing module receives the excitation signal, it acquires the first signal corresponding to the excitation signal. After acquiring the first signal corresponding to the excitation signal, the first signals are averaged to obtain an electrical signal and a motion signal, wherein the motion signal is the average of all first motion signals of the cell, and the electrical signal is the average of all first electrical signals of the cell; a weak signal analysis module is coupled to the output of the signal processing module to receive the first motion signal and the first electrical signal; the weak signal analysis module performs weak signal analysis on the motion signal and the electrical signal respectively.

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