Prussian blue nano test strip for detecting African horse pestivirus antibody as well as preparation method and application of Prussian blue nano test strip

By developing antibody test strips based on Prussian blue nanoparticles, and utilizing the specific reaction of the VP7 protein, the problem of rapid detection of African horse sickness virus antibodies at the grassroots level has been solved, achieving a simple and highly sensitive detection effect.

CN121978329APending Publication Date: 2026-05-05ANIMAL AND PLANT & FOOD DETECTION CENTER JIANGSU ENTRY EXIT INSPECTION AND QUARANTINE BUREAU +1
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Patent Information

Application Number
CN202610198212.8
Authority / Receiving Office
CN · China
Patent Type
Applications(China)
Current Assignee / Owner
Filing Date
2026-02-11
Publication Date
2026-05-05

AI Technical Summary

Technical Problem

Existing technologies are difficult to widely apply at the grassroots level for rapid and accurate detection of African horse sickness virus, and laboratory testing methods are limited by equipment and professional personnel.

Method used

An antibody test strip based on Prussian blue nanoparticles was developed. It utilizes the specific reaction of VP7 protein to achieve rapid detection through immunochromatography. The antibody detection is performed using a recombinant protein fragment of African horse sickness virus VP7 ΔN1-129 and a monoclonal antibody.

Benefits of technology

It enables rapid, simple, and sensitive detection of African horse sickness virus antibodies, suitable for grassroots and field testing, improving the specificity and sensitivity of the test and reducing the occurrence of false negative results.

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Abstract

The invention belongs to the technical field of biology, and particularly relates to a Prussian blue nano test strip for detecting an African horse pestivirus antibody as well as a preparation method and application of the Prussian blue nano test strip. A sample pad, a combination pad, a chromatography detection membrane and a water absorption pad are sequentially fixed on the supporting bottom plate; the chromatography detection membrane is provided with a detection line and a quality control line, the detection line is coated with staphylococcus A protein, and the quality control line is coated with a monoclonal antibody of African horse pestivirus VP7 recombinant protein; and the combination pad is coated with an African horse pestivirus VP7 delta N1-129 recombinant protein fragment coupled with Prussian blue nanoparticles. According to the invention, Prussian blue nano is used as a coupling tracer, and the specific reaction of an antigen and an antibody is fully utilized to detect the African horse pestivirus antibody. After the conjugate is combined with a corresponding antibody, a result can be clearly observed, and the conjugate is good in stability, high in sensitivity and strong in specificity, has a clinical application function and huge development potential, and is an extremely ideal antibody test strip detection material.
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