Dual-mode cooperative detection method for unsaturated fatty acid in Baijiu

By using a modified C18 solid-phase extraction column and low-temperature anti-oxidation derivatization technology, combined with gas chromatography-mass spectrometry and high-performance liquid chromatography-quadrupole time-of-flight mass spectrometry, the problem of simultaneous detection of unsaturated fatty acids in baijiu was solved. This achieved high-fidelity quantification of macro-components and high-sensitivity detection of trace components, making it suitable for batch detection of baijiu with different aroma types and alcohol contents.

CN121994970APending Publication Date: 2026-05-08JING BRAND
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Patent Information

Application Number
CN202610305811.5
Authority / Receiving Office
CN · China
Patent Type
Applications(China)
Current Assignee / Owner
Filing Date
2026-03-13
Publication Date
2026-05-08

AI Technical Summary

Technical Problem

Existing technologies cannot simultaneously achieve high-fidelity quantification of easily oxidizable macro-components and high-sensitivity detection of trace easily oxidizable components in the same baijiu sample. Furthermore, they suffer from matrix interference and oxidative degradation issues, failing to meet the needs of batch and standardized testing in the baijiu industry.

Method used

Selective enrichment and matrix purification were achieved using modified C18 solid-phase extraction columns, combined with low-temperature anti-oxidative derivatization and dual-mode synergistic detection. Palmitic acid, oleic acid, linoleic acid, and α-linolenic acid were detected using gas chromatography-mass spectrometry (GC-MS) and high-performance liquid chromatography-quadrupole time-of-flight mass spectrometry (HPLC-QFS-MS). Derivatization reactions were carried out at low temperatures using composite derivatization reagents and antioxidant auxiliaries, and the results were calibrated using internal and external standard methods.

Benefits of technology

This method enables efficient and simultaneous detection of unsaturated fatty acids in baijiu (Chinese liquor), effectively removing matrix interference, reducing oxidation loss, and improving the accuracy and stability of detection. It is suitable for batch detection of baijiu with different aroma types and alcohol contents.

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Abstract

The invention discloses a dual-mode cooperative detection method for unsaturated fatty acid in Baijiu, and relates to the technical field of food detection. According to the method, aiming at the characteristic of a high alcohol ester matrix of the white spirit and the detection pain point that unsaturated fatty acid is easy to oxidize and large in content span, selective enrichment and matrix purification are performed on a white spirit sample through a silanization modified C18 solid-phase extraction small column, and then low-temperature anti-oxidation derivatization is completed through matching of a composite derivatization reagent and an anti-oxidation system. Then, the palmitic acid, the oleic acid and the linoleic acid are quantified by adopting a gas chromatography-mass spectrometry method, meanwhile, the alpha-linolenic acid is directly detected by adopting a high performance liquid chromatography-quadrupole time-of-flight mass spectrometry method, and finally, results are calibrated and output by combining an internal standard method and an external standard method. According to the method, efficient purification of the Baijiu matrix is achieved, the oxidation loss rate of alpha-linolenic acid is controlled within 3%, high-precision quantification of constant components and high-sensitivity detection of trace components can be synchronously achieved, the method is suitable for detection of full-flavor and full-alcoholic-degree Baijiu, stability and universality are excellent, and reliable technical support is provided for Baijiu quality evaluation.
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