Skin care composition aiming at periocular senescence problem of sensitive skin and application of skin care composition

Abstract

The invention relates to the technical field of skin care products, in particular to a skin care composition for the periocular aging problem of sensitive skin and application of the skin care composition. The skin care composition comprises the following components: acetyl hexapeptide-8, a roselle flower extract, a watermelon fruit extract and a tephrosia rubescens seed extract, and the four components act on different mutually associated physiological pathways to generate a remarkable synergistic effect. A synergistic network covering a full chain of'neural signal input-metabolic waste removal-matrix structure reconstruction-structure protection and defense 'is formed, the depth of periorbital wrinkles can be remarkably reduced, the number of periorbital wrinkles is reduced, under-eye puffiness and periorbital edema are eliminated, dark circles are faded, the skin compactness and elasticity are improved, and the eye protection effect is improved. And a comprehensive and efficient eye rejuvenation effect is achieved.

Description

Technical Field

[0001] This invention relates to the technical field of skincare products, specifically to a skincare composition for sensitive skin and its application around the eyes. Background Technology

[0002] The periocular region, being the thinnest and most delicate area of ​​human skin, possesses highly unique anatomical structures and physiological functions. The dermis in this area is only about one-third to one-quarter the thickness of other facial areas, and the connective tissue is looser with less dense collagen fiber arrangement compared to other parts of the face. This makes it less tolerant to external mechanical stress and internal physiological changes. Simultaneously, the periocular region is rich in capillary networks and lymphatic vessels, responsible for the precise delivery of nutrients and the removal of metabolic waste. However, due to the weak venous valve structure, relatively insufficient lymphatic drainage, and a weakened skin barrier function (sparse distribution of sebaceous and sweat glands, and an incomplete sebum film), the periocular region is highly susceptible to circulatory disorders and water metabolism imbalances.

[0003] From a dynamic physiological perspective, the high-frequency contraction of facial muscles such as the orbicularis oculi is essential for life activities. However, long-term, repetitive muscle movements lead to repeated skin folding, which in turn induces micro-damage to the dermal matrix structure and eventually solidifies into visible wrinkles. Furthermore, the skin around the eyes is exposed to continuous internal aging processes (such as slowed cell renewal, decline in the antioxidant defense system, and accumulation of glycation) and external environmental stresses (such as ultraviolet radiation, blue light, and pollution). These factors work together on a fragile base, synergistically leading to a series of complex periorbital aesthetic problems such as eye bags, edema, dark circles, and wrinkles. These problems are often interconnected and mutually causal. For example, poor microcirculation not only causes edema and vascular dark circles but also accelerates skin aging and abnormal pigment metabolism due to insufficient nutrient supply and accumulation of metabolic waste. Meanwhile, skin laxity and thinning caused by dermal matrix degradation deepen the shadows of structural dark circles and exacerbate the appearance of wrinkles.

[0004] Current eye care products on the market targeting these issues often focus on single or limited pathways of action for their active ingredients. For example, they may use neurotransmitter analogs to inhibit muscle contraction, vitamin K or caffeine to improve local circulation, or retinol and its derivatives to promote collagen regeneration. However, simply combining these ingredients often fails to produce significant synergistic effects and may not comprehensively address the multiple concerns around the eyes due to their limited target. For instance, while inhibiting muscle contraction can improve dynamic wrinkles, its effectiveness is limited for edema and vascular dark circles caused by circulatory disorders. Ingredients that only improve microcirculation are insufficient for repairing existing dermal matrix damage and deep wrinkles. Furthermore, many potent ingredients, such as high-concentration retinol, may irritate sensitive skin around the eyes, leading to tolerance issues.

[0005] In summary, due to the multifactorial nature of periorbital problems, a comprehensive solution is needed that can simultaneously address multiple key pathophysiological processes. Therefore, developing an eye care composition that achieves synergistic effects across multiple targets while possessing both high efficacy and gentleness has become a pressing technical challenge in this field. Summary of the Invention

[0006] The purpose of this invention is to overcome the above-mentioned defects of the prior art and provide a novel skin care composition that can improve periorbital skin problems through the synergistic effect of multiple active ingredients via multiple pathways.

[0007] To achieve the above objectives, the technical solution adopted by the present invention is as follows: In a first aspect, the present invention provides a skin care composition for sensitive skin with periorbital aging problems, comprising the following components: acetyl hexapeptide-8, hibiscus flower extract, watermelon fruit extract and edamame seed extract, wherein the weight ratio of acetyl hexapeptide-8, hibiscus flower extract, watermelon fruit extract and edamame seed extract is (0.01-5):(0.1-5):(0.1-5):(0.01-1).

[0008] Acetyl hexapeptide-8 is a biomimetic hexapeptide derived from the SNAP-25 protein fragment. Its core mechanism of action lies in reversibly inhibiting the excessive release of catecholamines and acetylcholine at the neuromuscular junction. By competitively binding to the SNARE complex, it interferes with the fusion process of vesicles and cell membranes, thereby gently and precisely blocking the transmission of nerve signals that trigger muscle contraction. This mechanism can reduce skin folding caused by repeated and excessive contraction of facial muscles such as the orbicularis oculi from the source, effectively preventing and reducing dynamic expression lines, and providing relief for poor local circulation that may be exacerbated by muscle tension.

[0009] Roselle flower extract is rich in anthocyanins, flavonoids, and other polyphenolic active substances. Its mechanism of action is mainly manifested in its powerful antioxidant properties and inhibition of the formation of advanced glycation end products (AGEs). The polyphenolic active substances it contains can effectively scavenge free radicals and reduce oxidative stress damage. At the same time, as an active carbonyl scavenger, it blocks the non-enzymatic glycation reaction between reducing sugars and proteins (such as collagen and elastin), preventing the cross-linking, hardening, and discoloration of skin matrix proteins. Roselle flower extract helps maintain skin elasticity by protecting the integrity and function of key structural proteins in the dermis, improves dull skin tone caused by glycation, and indirectly supports the health of the skin barrier.

[0010] The mechanism of action of watermelon fruit extract focuses on improving microcirculation and lymphatic drainage around the eyes. Its active ingredients, such as citrulline, promote endothelial dilation, enhance capillary blood flow velocity and perfusion, thereby accelerating the transport of oxygen and nutrients and the removal of metabolic waste. At the same time, it can stimulate the pumping function of lymphatic vessels, effectively promoting the return of excess lymph and protein retained in the interstitial space. This dual action directly addresses the problems of vascular stasis and poor lymphatic return, and is a key pathway to quickly relieve morning or fatigue-induced edema-type eye bags and lighten bluish-purple vascular dark circles caused by hemoglobin deposition.

[0011] The extract of grey soybean seed mainly acts on the structural remodeling and strengthening of the dermis. Its core mechanism lies in significantly upregulating the expression of genes that synthesize type I and type III collagen and fibronectin in skin fibroblasts, promoting the secretion and deposition of these key extracellular matrix components. By increasing the density of the dermis and strengthening the anchoring structure of the dermal-epidermal junction, the extract of grey soybean seed can fundamentally thicken and strengthen the skin support network, thereby effectively improving static wrinkles, structural eye bags and shadows, and overall skin fragility caused by skin laxity and thinning.

[0012] Preferably, the weight ratio of acetyl hexapeptide-8, hibiscus flower extract, watermelon fruit extract and edamame seed extract is (0.1-3):(0.5-3):(0.5-3):(0.05-0.8).

[0013] More preferably, the weight ratio of acetyl hexapeptide-8, hibiscus flower extract, watermelon fruit extract and edamame seed extract is (0.5-1.5):(1-1.5):(1-2):(0.1-0.5).

[0014] Secondly, the present invention provides the application of the skin care composition described in the first aspect for addressing periorbital aging in sensitive skin in the preparation of skin care products.

[0015] Preferably, the skin care product is any one of toner, lotion, cream, mask, or freeze-dried powder.

[0016] Preferably, the amount of the skin care composition added is 1%-15% of the total weight of the skin care product.

[0017] Thirdly, the present invention provides an eye cream comprising the following ingredients by weight percentage: 1%-15% of the skin care composition described in the first aspect, 0.01%-1% of a thickener, 1%-10% of a moisturizer, 0.1%-6% of an emulsifier, 1%-15% of oils, 0.1%-2% of a preservative, and 0.01%-0.5% of a pH adjuster, with the balance being deionized water.

[0018] Preferably, the eye cream comprises the following ingredients by weight percentage: 5%-10% of the skin care composition described in the first aspect, 0.1%-0.5% thickener, 2%-8% moisturizer, 0.1%-5% emulsifier, 5%-10% oil, 0.5%-2% preservative, and 0.01%-0.3% pH adjuster, with the balance being deionized water.

[0019] More preferably, the thickener includes at least one of xanthan gum, carbomer, hydroxyethyl acrylate / sodium acryloyldimethyl taurate copolymer, ammonium acryloyldimethyl taurate / VP copolymer, and sclerotium gum.

[0020] More preferably, the moisturizer includes at least one of allantoin, betaine, β-glucan, trehalose, caprylyl glycol, dipropylene glycol, sodium hyaluronate, 1,3-butanediol, glycerin, D-panthenol, and ceramide.

[0021] More preferably, the emulsifier includes at least one of C14-22 alcohol, C12-20 alkyl glucoside, cetearyl glucoside, and sucrose stearate.

[0022] More preferably, the oil comprises at least one of caprylic / capric triglyceride, isononyl isononanoate, pentaerythritol tetraester, polydimethylsiloxane, stearyl alcohol, hydroxystearic acid, polymethylsilsesquioxane, and pentaerythritol distearate.

[0023] More preferably, the preservative includes at least one of 1,3-propanediol, 1,2-hexanediol, and p-hydroxyacetophenone.

[0024] More preferably, the pH adjuster includes at least one of arginine, disodium ethylenediaminetetraacetate, tromethamine, and triethanolamine.

[0025] Fourthly, the present invention provides a method for preparing the eye cream described in the third aspect, comprising the following steps: S1. Mix the thickener, humectant and deionized water evenly, heat to 82-87℃ and homogenize at 1000-1500 rpm for 3-5 minutes. After homogenization, keep warm for later use to obtain the pre-made component A. S2. Mix the oil and emulsifier, heat to 82-87℃, and homogenize at 1000-1500 rpm for 3-5 minutes. After homogenization, keep warm for later use to obtain the pre-made component B. S3. Mix the preservatives and heat to 58-62℃ to melt them, to obtain the pre-prepared component C; S4. Heat pre-prepared component A to 78-82℃, add pre-prepared component B at 200-300 rpm, stir and mix, cool to 58-62℃, add pre-prepared component C at 200-300 rpm, stir and mix, cool to 38-42℃, add all components of the skin care composition described in the first aspect at 200-300 rpm, continue stirring for 5-10 minutes, finally add pH adjuster to adjust pH, stop stirring, discharge the material, and obtain the eye cream.

[0026] Compared with the prior art, the beneficial effects of the present invention are as follows: This invention provides a skincare composition targeting periorbital aging in sensitive skin. It effectively combines acetyl hexapeptide-8, hibiscus flower extract, watermelon fruit extract, and edamame seed extract. These four ingredients work through different, interconnected physiological pathways to produce a significant synergistic effect. The resulting skincare composition can effectively alleviate and even prevent expression lines and static wrinkles around the eyes, reduce eye bags and edema, and lighten different types of dark circles. Simultaneously, it achieves multiple biological effects, including inhibiting neuromuscular contraction, promoting capillary blood flow and lymphatic drainage, inhibiting the formation of advanced glycation end products (AGEs), and stimulating the synthesis of collagen and extracellular matrix in the dermis. This fundamentally improves the youthful appearance of the periorbital skin and has good skin tolerance. Among them, acetyl hexapeptide-8 creates a favorable mechanical environment for improving microcirculation by relaxing muscles; watermelon fruit extract delivers nutrients to tissues and removes metabolic waste by accelerating circulation and lymphatic drainage, providing an optimized microenvironment for cell repair (such as collagen synthesis promoted by edamame seed extract), while directly addressing edema and vascular dark circles; hibiscus flower extract protects newly synthesized and existing collagen from damage through anti-glycation and antioxidant effects, consolidating and prolonging the structural remodeling effects of edamame seed extract, and improving skin tone. These four components do not act independently, but rather form a synergistic network covering the entire chain of "neural signal input - metabolic waste removal - matrix structure reconstruction - structural protection and defense." Ultimately, this synergistic system can significantly reduce the depth and number of wrinkles around the eyes, diminish eye bags and puffiness, lighten dark circles, and improve skin firmness and elasticity, achieving a comprehensive and efficient eye area rejuvenation effect. Attached Figure Description

[0027] Figure 1 The images show the improvement in dark circles, eye bags, and fine lines in volunteers who used the eye cream in Example 1 for 0 and 28 days, respectively, in Test Example 3. Detailed Implementation

[0028] To better illustrate the purpose, technical solution, and advantages of the present invention, the present invention will be further described below in conjunction with specific embodiments.

[0029] The sources of the raw materials used in the following examples and comparative examples are as follows: Acetyl hexapeptide-8 was purchased from Nanjing Leon Biotechnology Co., Ltd., CAS No.: 616204-22-9; Hibiscus flower extract was purchased from GREENTECH, under the brand name FLOWER ACIDS. ® ; The watermelon fruit extract was purchased from Fufeng Sinote Biotechnology Co., Ltd., and its trade name is Watermelon Extract. The edamame seed extract was purchased from Givaudan, Switzerland, under the trade name Neurophroline.

[0030] Unless otherwise specified, all other materials and reagents used in the examples are commercially available.

[0031] The component composition (parts by weight) of the skin care compositions for sensitive skin and periorbital aging problems in Examples 1-7 of this invention is shown in Table 1 below. Each component was weighed according to the formula amount, mixed and homogenized to obtain each skin care composition.

[0032] Table 1. Components and their weight parts of the skin care compositions in Examples 1-7 Group Acetyl hexapeptide-8 (parts by weight) Hibiscus flower extract (parts by weight) Watermelon fruit extract (parts by weight) Edamame seed extract (parts by weight) Example 1 1.1 1.2 1.5 0.3 Example 2 0.5 1 1 0.1 Example 3 1.5 1.5 2 0.5 Example 4 0.1 0.5 0.5 0.05 Example 5 3 3 3 0.8 Example 6 0.01 0.1 0.1 0.01 Example 7 5 5 5 1 Comparative Example 1 The difference between Comparative Example 1 and Example 1 is that Acetyl hexapeptide-8 was not added in Comparative Example 1, and the missing amount was made up by hibiscus flower extract, watermelon fruit extract and edamame seed extract in a weight ratio of 1.2:1.5:0.3.

[0033] Comparative Example 2 The difference between Comparative Example 2 and Example 1 is that: in Comparative Example 2, hibiscus flower extract was not added, and acetyl hexapeptide-8, watermelon fruit extract and edamame seed extract in a weight ratio of 1.1:1.5:0.3 were used to make up for the missing amount.

[0034] Comparative Example 3 The difference between Comparative Example 3 and Example 1 is that watermelon fruit extract was not added in Comparative Example 3, and acetyl hexapeptide-8, hibiscus flower extract and edamame seed extract in a weight ratio of 1.1:1.2:0.3 were used to make up for the missing amount.

[0035] Comparative Example 4 The difference between Comparative Example 4 and Example 1 is that Comparative Example 4 does not add edamame seed extract, but uses acetyl hexapeptide-8, hibiscus flower extract and watermelon fruit extract in a weight ratio of 1.1:1.2:1.5 to make up for the missing amount.

[0036] Comparative Example 5 The difference between Comparative Example 5 and Example 1 is that Comparative Example 5 uses acetyl hexapeptide-8, hibiscus flower extract, watermelon fruit extract and edamame seed extract in a weight ratio of 0.3:1.2:1.5:1.1.

[0037] Comparative Example 6 The difference between Comparative Example 6 and Example 1 is that Comparative Example 6 uses acetyl hexapeptide-8, hibiscus flower extract, watermelon fruit extract and edamame seed extract in a weight ratio of 1.1:0.3:1.5:1.2.

[0038] Comparative Example 7 The difference between Comparative Example 7 and Example 1 is that Comparative Example 7 uses acetyl hexapeptide-8, hibiscus flower extract, watermelon fruit extract and edamame seed extract in a weight ratio of 1.1:1.2:0.3:1.5.

[0039] The total weight of the skin care compositions in Examples 1-7 and Comparative Examples 1-7 above is equal.

[0040] Test Example 1: Anti-wrinkle ability test of skincare composition In the process of skin anti-wrinkle and rejuvenation, type I collagen, type III collagen, and fibronectin are three core structural proteins that constitute the extracellular matrix of the dermis. Together, they form a three-dimensional network system that maintains skin strength, elasticity, and firmness, with each protein performing its specific function while working in close coordination. Therefore, this test case evaluates the anti-wrinkle ability of the skincare compositions by testing the expression promotion rate of the above three proteins by the skincare compositions prepared in Examples 1-7 and Comparative Examples 1-7.

[0041] The cell line used was human dermal fibroblasts (HDF) (Guangzhou Genio Biotechnology Co., Ltd.). The test conditions were: incubator temperature 37±1℃, humidity 90±5%, carbon dioxide 5±1%. Cells were cultured and treated according to groups, followed by testing. The test methods are as follows: (1) Sample preparation: The skin care compositions prepared in Examples 1-7 and Comparative Examples 1-7 were dissolved in DMSO and then diluted with DMEM medium. The final experimental concentration was 0.1% (v / v). (2) Resuscitating HDF cells: The cell suspension was seeded into 96-well cell culture plates at a density of 2 × 10⁶ cells per well. 3 Add 100 μL of DMEM medium containing 10% (v / v) fetal bovine serum and 1% penicillin-streptomycin solution to each well and revive and culture for 24 h; (3) Sample feeding: Discard the supernatant, add 100 μL of DMEM medium to each well of the blank group; add 100 μL of DMEM medium containing 0.1% (v / v) sample to each well of the sample group, culture for 24 h, collect cells from each group, discard the medium, wash twice with PBS; add 100 μL of TRIzol lysis buffer to each well, scrape the cells, transfer them to EP tubes, and store at -80℃ for later use; (4) Protein content detection by ELISA: The content of type I and type III collagen and fibronectin in each group was detected by human type I collagen (Col I) ELISA kit (Shanghai Enzyme-Link Biotechnology Co., Ltd., catalog number ml057630), human type III collagen (Col III) ELISA kit (Shanghai Jianglai Biotechnology Co., Ltd., JL14257-48T) and human cell fibronectin (cFn) ELISA kit (Shanghai Keaibo Biotechnology Co., Ltd., CB11460-Hu); (5) Calculate the protein expression promotion rate according to the following formula. The data are presented as average values. The results are shown in Table 2. Protein expression promotion rate = (protein expression level) 样品组 - Protein expression level 空白组 / Protein expression level 空白组 ×100%; Table 2. Test data on the anti-wrinkle ability of skincare compositions Group Type I collagen expression promotion rate (%) Type III collagen expression promotion rate (%) Fibronectin expression promotion rate (%) Example 1 83.4 88.6 77.9 Example 2 80.7 86.5 74.6 Example 3 81.2 85.3 75.8 Example 4 77.9 83.7 72.6 Example 5 78.6 83.1 73.0 Example 6 76.5 81.2 70.9 Example 7 75.8 80.7 71.3 Comparative Example 1 55.1 58.6 50.8 Comparative Example 2 49.2 55.4 47.6 Comparative Example 3 51.7 53.2 45.4 Comparative Example 4 54.9 58.1 51.0 Comparative Example 5 73.0 77.5 69.2 Comparative Example 6 71.4 75.8 67.5 Comparative Example 7 72.6 74.3 66.7 Table 2 shows the data on the promotion rates of type I collagen expression, type III collagen expression, and fibronectin expression in Examples 1 and Comparative Examples 1-4. It is evident that the skincare composition prepared using a combination of four ingredients—acetyl hexapeptide-8, hibiscus flower extract, watermelon fruit extract, and edamame seed extract—significantly improved the promotion rates of type I collagen expression, type III collagen expression, and fibronectin expression compared to the skincare composition prepared using any three of these ingredients. This indicates that acetyl hexapeptide-8, hibiscus flower extract, watermelon fruit extract, and edamame seed extract have a synergistic effect.

[0042] Based on the data in Table 2 regarding the promotion rates of type I collagen expression, type III collagen expression, and fibronectin expression in Examples 1-7 and Comparative Examples 5-7, the synergistic effect of the skincare composition is better when the weight ratio of acetyl hexapeptide-8, hibiscus flower extract, watermelon fruit extract, and edamame seed extract is (0.01-5):(0.1-5):(0.1-5):(0.01-1). Furthermore, the skincare composition exhibits the best effect when the weight ratio of acetyl hexapeptide-8, hibiscus flower extract, watermelon fruit extract, and edamame seed extract is 1.1:1.2:1.5:0.3.

[0043] Application Example 1-7 and Comparative Application Example 1-8 The skincare compositions of Examples 1-7 and Comparative Examples 1-7 were added to the eye cream at a concentration of 10 wt% to obtain the eye creams of Application Examples 1-7 and Comparative Application Examples 1-7. The formulations are shown in Table 3.

[0044] The preparation methods of the eye creams in Application Examples 1-7 and Comparative Application Examples 1-7 include the following steps: S1. Mix the thickener, humectant and deionized water evenly, heat to 85°C and homogenize at 1200 rpm for 4 minutes. After homogenization, keep warm for later use to obtain the pre-made component A. S2. Mix the oil and emulsifier, heat to 85°C, and homogenize at 1200 rpm for 4 minutes. After homogenization, keep warm for later use to obtain the pre-made component B. S3. Mix the preservatives and heat to 62°C to melt them, to obtain the pre-prepared component C. S4. Heat pre-prepared component A to 80°C, add pre-prepared component B at 250 rpm, stir and mix, cool to 60°C, add pre-prepared component C at 250 rpm, stir and mix, cool to 40°C, add all components of the skin care composition at 250 rpm, continue stirring for 8 minutes, finally add pH adjuster to adjust pH, stop stirring, discharge the material, and obtain the eye cream.

[0045] Table 3. Eye cream formulations of Application Examples 1-7 and Comparative Application Examples 1-7

[0046] Compare with application example 8 (blank application example) The difference between Comparative Application Example 8 and Application Example 1 is that the eye cream in Comparative Application Example 8 does not contain the skin care composition, but uses an equal amount of deionized water instead of the skin care composition, and the preparation method is the same as in Application Example 1.

[0047] Test Example 2: Human Skin Patch Test To rigorously verify the high safety and gentleness of the eye cream prepared using the skin care composition of this application on sensitive skin, this study designed and implemented an enhanced human occlusive patch test.

[0048] The trial recruited 160 volunteers aged 20 to 50 (50% of whom reported having sensitive skin), with an equal number of men and women, and randomly divided into 16 groups of 10 each. All participants signed informed consent forms. To simulate the barrier damage commonly seen in sensitive skin, the trial pre-existed a more stringent testing environment by repeatedly tearing medical 3M tape five times on the flexor surface of the participants' arms. Subsequently, equal amounts of 0.020 g of test eye cream samples (Application Examples 1-7, Control Application Examples 1-7, Control Application Example 8 (blank application example) and purified water (negative control group) were placed in 10-well patch applicators and applied to the pre-treated skin with hypoallergenic tape for 24 hours. At 0, 24 and 48 hours after the patch was removed, a professional assessor observed and recorded the skin reaction according to the adverse reaction level (see Table 4). The final safety judgment standard was: if the average irritation score of the application group was not higher than that of the blank control group, and no adverse reaction of grade 2 or above occurred, it was proven that the product was safe and non-irritating and suitable for sensitive skin.

[0049] Table 4. Adverse skin reaction grades Rating levels Skin reaction 0 negative reaction 1 Suspicious reaction, only slight erythema 2 Weak positive reaction (erythema reaction): erythema, infiltration, edema, and papules may be present. 3 Strong positive reaction (herpes reaction): erythema, infiltration, edema, papules; the reaction may extend beyond the test area. 4 Extremely strong positive reaction (confluent herpes simplex reaction): obvious erythema, severe infiltration, edema, confluent herpes simplex; reaction extends beyond the test area. The results of the human skin patch test showed that the skin reactions of volunteers in the application examples 1-7 and the control examples 1-8 were observed at 0 h, 24 h and 48 h. No adverse skin reactions were observed in any of them, and all were negative reactions. This indicates that the eye cream prepared by the skin care composition provided by the present invention is safe and does not irritate human skin.

[0050] Test Example 3: Human Efficacy Test Experimental Methods: Based on the principles of human efficacy evaluation in the "Cosmetic Safety Technical Specifications" (2015 edition), this experiment aims to scientifically evaluate the improvement effect of the samples (eye creams prepared in Application Examples 1-7 and Comparative Application Examples 1-8) on healthy subjects with moderate to severe eye bags, edema, dark circles and wrinkles.

[0051] Subjects: 150 subjects (10 per group) aged 35-55 years in good health were recruited. All subjects signed informed consent forms. Exclusion criteria included serious systemic diseases, recent eye surgery or injections, and allergies to the ingredients.

[0052] Directions for use: After cleansing your face in the morning and evening, take 0.5g of the sample and apply it to both eye areas. Gently massage until absorbed. Continue use for 28 days.

[0053] Testing: Volunteers in each group underwent instrument testing on day 0 (D0 baseline period) and day 28 (D28 trial endpoint). All tests were conducted after the volunteers cleaned their faces with a uniform cleanser and sat quietly for 30 minutes in the experimental environment (temperature 20±1℃, humidity 50±10%).

[0054] Efficacy indicators (objective instrument measurement): a) Eye bags: Quantitative analysis of volume changes (mm³) in the lower eyelid area using a 3D facial imaging system (VECTRA M3); b) Dark circles: Analysis of dark circles under the eyes using VISIA-CR imaging; c) Wrinkles: Dynamic wrinkles: Recorded using a high-speed camera system under standardized smile inducement, and the average depth of wrinkles in the outer canthus area was analyzed by professional software; Fine lines under the eyes: Analysis of the area of ​​fine lines under the eyes using VISIA-CR imaging; d) Periocular firmness and elasticity: Measurement of the skin firmness parameter F4 and skin elasticity parameter R2 of the fundus using a Cutometer (MPA580, Courage and Khazaka, Germany).

[0055] The calculation formulas for each test indicator are as follows: Dark circle improvement rate (%) = (dark circles) D0 -Dark circles D28 Dark circles D0 ×100%; In the formula: Dark circles D0 Data on dark circles under the eyes on day 0; Dark circles D28 Data on dark circles under the eyes on day 28; Dynamic wrinkle improvement rate (%) = (Dynamic wrinkle) D0 -Dynamic texture D28 ) / Dynamic Texture D0 ×100%; In the formula: Dynamic texture D0 This is the dynamic texture data for day 0; Dynamic texture D28 This is the dynamic texture data for day 28; Fine line improvement rate (%) = (fine lines D0 Fine lines D28 Fine lines D0 ×100%; In the formula: Fine lines D0 This is the fine line data for day 0; Fine lines D28 This is the data for fine lines on day 28; Improvement rate of eye bags (%) = (eye bags) D0 - Eye bagsD28 ) / eye bags D0 ×100%; In the formula: eye bags D0 Data on eye bags on day 0; eye bags D28 Data on under-eye bags on day 28; F4 value improvement rate (%) = (F4 value) D0 -F4 value D28 ) / F4 value D0 ×100%; In the formula: F4 value D0 This is the F4 value data for day 0; F4 value D28 This is the F4 value data for day 28; R² improvement rate (%) = (R² value) D28 -R2 value D0 R2 value D0 ×100%; In the formula: R2 value D0 This is the R² value data for day 0; R2 value D28 The R² value data is for day 28. The calculation results are averaged, as shown in Table 5.

[0056] Table 5. Human efficacy test data for each group of eye creams Group Dark circle improvement rate (%) Improvement rate of dynamic wrinkles (%) Fine line improvement rate (%) Improvement rate of eye bags (%) F4 value improvement rate (%) R2 improvement rate (%) Application Example 1 32.6 23.4 27.0 25.9 28.7 30.1 Application Example 2 30.8 22.1 26.3 24.6 26.3 27.8 Application Example 3 31.2 22.9 25.8 25.2 27.0 28.9 Application Example 4 29.9 20.8 24.2 22.8 24.8 26.5 Application Example 5 29.0 21.2 23.4 23.1 25.6 25.7 Application Example 6 27.1 19.3 21.1 20.7 23.5 23.4 Application Example 7 28.5 18.7 22.5 21.3 22.9 24.3 Comparative Application Example 1 14.4 7.8 10.4 10.2 9.4 10.5 Comparative Application Example 2 12.8 10.2 8.6 9.4 10.0 8.7 Comparative Application Example 3 13.1 9.7 7.3 7.7 10.9 11.8 Comparative Application Example 4 11.9 9.4 9.7 8.5 8.1 8.4 Comparative Application Example 5 18.5 15.6 19.2 18.9 15.8 18.2 Comparative Application Example 6 19.7 16.9 18.7 17.6 16.9 17.5 Comparative Application Example 7 21.1 17.2 16.8 15.7 17.3 19.6 Comparative Application Example 8 0.8 0.5 0.6 0.4 0.2 0.3 Table 5 shows the improvement rates of dark circles, dynamic wrinkles, fine lines, eye bags, F4, and R2 in Application Example 1 and Comparative Application Examples 1-4. The results indicate that the eye cream prepared using a skincare composition combining acetyl hexapeptide-8, hibiscus flower extract, watermelon fruit extract, and edamame seed extract exhibits high improvement rates in dark circles, dynamic wrinkles, under-eye fine lines, and eye puffiness. This demonstrates that the four ingredients—acetyl hexapeptide-8, hibiscus flower extract, watermelon fruit extract, and edamame seed extract—work synergistically, enhancing the skincare composition's ability to improve eye skin problems such as dark circles, wrinkles, and eye bags.

[0057] Based on the data in Table 5 regarding the improvement rates of dark circles, dynamic wrinkles, fine lines, eye bags, F4, and R2 in Application Examples 1-7 and Comparative Application Examples 5-7, it can be concluded that the synergistic effect of the skincare composition is better when the weight ratio of acetyl hexapeptide-8, hibiscus flower extract, watermelon fruit extract, and edamame seed extract is (0.01-5):(0.1-5):(0.1-5):(0.01-1); and the skincare composition has the best effect when the weight ratio of acetyl hexapeptide-8, hibiscus flower extract, watermelon fruit extract, and edamame seed extract is 1.1:1.2:1.5:0.3.

[0058] Finally, it should be noted that the above embodiments are only used to illustrate the technical solutions of the present invention and are not intended to limit the scope of protection of the present invention. Although the present invention has been described in detail with reference to preferred embodiments, those skilled in the art should understand that modifications or equivalent substitutions can be made to the technical solutions of the present invention without departing from the essence and scope of the technical solutions of the present invention.

Claims

1. A skincare composition targeting periorbital aging in sensitive skin, characterized in that, It includes the following components: acetyl hexapeptide-8, hibiscus flower extract, watermelon fruit extract and edamame seed extract, wherein the weight ratio of acetyl hexapeptide-8, hibiscus flower extract, watermelon fruit extract and edamame seed extract is (0.01-5):(0.1-5):(0.1-5):(0.01-1).

2. The skincare composition for sensitive skin and periorbital aging as described in claim 1, characterized in that, The weight ratio of acetyl hexapeptide-8, hibiscus flower extract, watermelon fruit extract, and edamame seed extract is (0.1-3):(0.5-3):(0.5-3):(0.05-0.8).

3. The skincare composition for sensitive skin and periorbital aging as described in claim 1, characterized in that, The weight ratio of acetyl hexapeptide-8, hibiscus flower extract, watermelon fruit extract and edamame seed extract is (0.5-1.5):(1-1.5):(1-2):(0.1-0.5).

4. The use of the skincare composition for sensitive skin and periorbital aging problems according to any one of claims 1-3 in the preparation of skincare products.

5. The application of the skincare composition for sensitive skin and periorbital aging as described in claim 4 in the preparation of skincare products, characterized in that, The skincare product is any one of toner, lotion, cream, mask, or freeze-dried powder, and the amount of the skincare composition added is 1%-15% of the total weight of the skincare product.

6. An eye cream, characterized in that, The ingredients comprise the following ingredients by weight percentage: 1%-15% of the skincare composition according to any one of claims 1-3, 0.01%-1% thickener, 1%-10% moisturizer, 0.1%-6% emulsifier, 1%-15% oil, 0.1%-2% preservative, and 0.01%-0.5% pH adjuster, with the balance being deionized water.

7. The eye cream as described in claim 6, characterized in that, The ingredients comprise the following ingredients by weight percentage: 5%-10% of the skincare composition according to any one of claims 1-3, 0.1%-0.5% thickener, 2%-8% moisturizer, 0.1%-5% emulsifier, 5%-10% oil, 0.5%-2% preservative, and 0.01%-0.3% pH adjuster, with the balance being deionized water.

8. The eye cream as described in any one of claims 6-7, characterized in that, The raw material is selected from at least one of (a)-(f): (a) The thickener comprises at least one of xanthan gum, carbomer, hydroxyethyl acrylate / sodium acryloyldimethyl taurate copolymer, ammonium acryloyldimethyl taurate / VP copolymer and sclerotium gum; (b) The moisturizer comprises at least one of allantoin, betaine, beta-glucan, trehalose, caprylyl glycol, dipropylene glycol, sodium hyaluronate, 1,3-butanediol, glycerin, D-panthenol and ceramide; (c) The emulsifier comprises at least one of C14-22 alcohol, C12-20 alkyl glucoside, cetearyl glucoside and sucrose stearate; (d) The oil comprises at least one of caprylic / capric triglyceride, isononyl isononanoate, pentaerythritol tetraester, polydimethylsiloxane, stearyl alcohol, hydroxystearic acid, polymethylsilsesquioxane and pentaerythritol distearate; (e) The preservative includes at least one of 1,3-propanediol, 1,2-hexanediol and p-hydroxyacetophenone; (f) The pH adjuster includes at least one of arginine, disodium ethylenediaminetetraacetate, tromethamine and triethanolamine.

9. The method for preparing the eye cream according to any one of claims 6-8, characterized in that, Includes the following steps: S1. Mix the thickener, humectant and deionized water evenly, heat to 82-87℃ and homogenize at 1000-1500 rpm for 3-5 minutes. After homogenization, keep warm for later use to obtain the pre-made component A. S2. Mix the oil and emulsifier, heat to 82-87℃, and homogenize at 1000-1500 rpm for 3-5 minutes. After homogenization, keep warm for later use to obtain the pre-made component B. S3. Mix the preservatives and heat to 58-62℃ to melt them, to obtain the pre-prepared component C; S4. Heat pre-prepared component A to 78-82℃, add pre-prepared component B at 200-300 rpm, stir and mix, cool to 58-62℃, add pre-prepared component C at 200-300 rpm, stir and mix, cool to 38-42℃, add each component of the skin care composition according to any one of claims 1-3 at 200-300 rpm, continue stirring for 5-10 minutes, finally add pH adjuster to adjust pH to 6.0, stop stirring, discharge the material, and obtain the eye cream.

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