Multiple primer combination and method for identifying mycobacteria and respiratory tract pathogenic bacteria and detecting drug-resistant genes of mycobacteria and respiratory tract pathogenic bacteria

By using multiple primer combinations and high-throughput sequencing technology, rapid and comprehensive identification and drug resistance gene detection of mycobacteria and respiratory pathogens have been achieved, solving the problems of long time consumption and low sensitivity in existing technologies and providing an efficient detection solution.

CN122012685APending Publication Date: 2026-05-12SICHUAN ACADEMY OF MEDICAL SCI SICHUAN PROVINCIAL PEOPLES HOSPITAL +1
0 Cites 0 Cited by

Patent Information

Application Number
CN202610304755.3
Authority / Receiving Office
CN · China
Patent Type
Applications(China)
Current Assignee / Owner
Filing Date
2026-03-13
Publication Date
2026-05-12

AI Technical Summary

Technical Problem

Existing methods for detecting mycobacteria and respiratory pathogens are time-consuming and have low sensitivity, failing to meet the needs of rapid clinical diagnosis and unable to comprehensively detect multiple mycobacteria and drug resistance genes.

Method used

By employing multiple primer combinations and methods, including nucleic acid extraction, reverse transcription, multiplex targeted amplification, adapter ligation, and library purification, combined with high-throughput sequencing technology, we can achieve the identification of mycobacteria and respiratory pathogens and the comprehensive screening of their drug resistance genes.

Benefits of technology

It enables comprehensive identification of 5 types of Mycobacterium tuberculosis, 34 types of non-tuberculous mycobacteria, and 55 types of respiratory pathogens, covering 2,000 drug resistance gene loci. It has a short detection cycle, high sensitivity and specificity, and is suitable for various sample types.

✦ Generated by Eureka AI based on patent content.
Patent Text Reader

Abstract

The invention relates to the technical field of detection, and provides a multiple primer combination and method for identifying mycobacteria and respiratory tract pathogenic bacteria and detecting drug-resistant genes of the mycobacteria and the respiratory tract pathogenic bacteria, and the nucleotide sequence of the primer combination comprises sequences as shown in SEQ ID NO: 1-SEQ ID NO: 380; the method comprises the following steps: nucleic acid extraction: extracting DNA and RNA in a sample as templates; reverse transcription: reversely transcribing the RNA into DNA (Deoxyribonucleic Acid); carrying out multi-target amplification, and configuring multi-PCR amplification; linker connection and library purification; and performing machine sequencing and data analysis. According to the application, pathogen identification is more comprehensive and accurate, and five kinds of mycobacterium tuberculosis, 34 kinds of nontuberculous mycobacteria and 55 kinds of common respiratory tract pathogenic bacteria are included; 2000 drug-resistant gene loci of clinically used anti-tuberculosis first-line and second-line drugs are comprehensively covered; all drug-resistant gene loci recommended by WHO and a guide can be comprehensively detected; the sensitivity and the specificity are high.
Need to check novelty before this filing date? Find Prior Art