Whitening composition and preparation method thereof

Abstract

The invention discloses a whitening composition and a preparation method thereof. The whitening composition is prepared from undecylenoyl phenylalanine, a chickweed extract, an ormosia henryi bark extract, an ascophyllum nodosum extract and a cystis ferruginea extract. The whitening composition provided by the invention has an excellent effect of inhibiting tyrosinase activity, is mild and non-irritant, and has a good popularization and application prospect.

Description

Technical Field

[0001] This invention belongs to the field of cosmetic technology, specifically relating to a whitening composition and its preparation method. Background Technology

[0002] Skin whitening and fading of blemishes is a continuously researched and focused area in the field of cosmetic technology. Skin color is primarily determined by the amount and distribution of melanin synthesized by melanocytes. In the biosynthesis of melanin, tyrosinase is the most critical rate-limiting enzyme, catalyzing the conversion of tyrosine to dopa, which is then oxidized to form melanin. Therefore, inhibiting tyrosinase activity is one of the most effective ways to achieve skin whitening and fade blemishes.

[0003] Currently, various tyrosinase inhibitors are used in skin-whitening cosmetics, such as arbutin, kojic acid, vitamin C, and its derivatives. However, these traditional skin-whitening ingredients have certain limitations in practical applications. For example, arbutin is structurally and physically unstable, easily decomposes in light, and its skin-whitening effect is only apparent at high concentrations; while kojic acid has a significant inhibitory effect, it has certain skin irritation and instability, which may lead to contact dermatitis, and it is easily oxidized and discolored in sunlight, limiting its widespread application; and vitamin C and its derivatives suffer from instability and are easily oxidized and inactivated.

[0004] Furthermore, as consumers increasingly demand higher levels of safety and gentleness in cosmetics, the market is urgently seeking highly effective yet gentle and low-irritant whitening ingredients. Many potent inhibitors are unsuitable for sensitive skin due to their potential irritation risks. Therefore, developing novel, gentle, and safe compound whitening compositions derived from natural plants has become an important direction in current cosmetic research and development.

[0005] Natural plant extracts have shown great potential in the development of skin whitening products due to their wide availability, gentle effects, and minimal side effects. However, current technologies mostly focus on the efficacy of single extracts or simply combine multiple extracts, failing to explore whether there are synergistic effects between different extracts. In particular, there are no publicly reported compositions that scientifically combine undecenoyl phenylalanine (a known melanocyte-stimulating hormone receptor antagonist) with the aforementioned specific natural extracts, optimizing the ratio to achieve excellent tyrosinase inhibition while also providing soothing, moisturizing, and non-irritating benefits.

[0006] Therefore, there is an urgent need in this field for a new technical solution that can overcome the shortcomings of existing whitening ingredients and provide a highly effective, stable, gentle and safe whitening composition. Summary of the Invention

[0007] The purpose of this invention is to overcome the shortcomings of the prior art and provide a composition that can effectively whiten skin while being gentle and non-irritating.

[0008] To achieve the above objectives, the present invention adopts the following technical solution: In a first aspect, the present invention provides a whitening composition comprising the following components in parts by weight: Undecenoyl phenylalanine: 0.1-0.5 parts; Emulsifier: 0.7-0.9 parts; Chickweed extract: 1-3 parts; Dalbergia odorifera bark extract: 3-5 parts; Laminaria japonica extract: 1-3 parts; Pteris reticulata extract: 0.5-0.7 parts; The emulsifier is at least one of cetearyl oleate, sorbitan oleate, and glyceryl stearate.

[0009] The present invention has found that the combined use of the above components has a synergistic effect, achieving excellent inhibitory efficacy against tyrosinase while being mild and non-irritating.

[0010] In the most preferred embodiment of the whitening composition of the present invention, the composition comprises the following components in parts by weight: Undecenoylphenylalanine: 0.3 parts; Emulsifier: 0.8 parts; Chickweed extract: 2 parts; Dalbergia odorifera bark extract: 4 parts; *Leptochloa crus-galli* extract: 2 parts; Pteris reticulata extract: 0.6 parts; The emulsifier is cetearyl oleate and sorbitan oleate in a mass ratio of 3:2.

[0011] The present invention has found that the mass fraction of each component in the composition affects the performance of the composition, and the composition with the best performance is obtained when the mass fraction of the components is further selected to be within the above-mentioned mass fraction.

[0012] Secondly, the present invention provides the use of the whitening composition described in the first aspect in the preparation of cosmetics with whitening effects.

[0013] Preferably, the dosage form of the cosmetic includes any one of lotion, serum, cream, mask, and spray.

[0014] Thirdly, the present invention provides a whitening serum, the serum comprising the whitening composition described in the first aspect.

[0015] Preferably, the essence further includes skin conditioning agents, preservatives, thickeners, and solvents.

[0016] Preferably, the skin conditioning agent includes at least one of glycerin, hyaluronic acid, sodium hyaluronate, and panthenol.

[0017] Preferably, the preservative includes at least one of phenoxyethanol, phenoxyethanol, potassium sorbate, and 1,2-hexanediol.

[0018] Preferably, the thickener includes at least one of gellan gum, sodium alginate, carbomer, xanthan gum, and sclerotium gum.

[0019] Preferably, the solvent is deionized water.

[0020] Compared with the prior art, the beneficial effects of the present invention are as follows: This invention provides a skin-whitening composition comprising, by a specific mass ratio, undecenoyl phenylalanine, chickweed extract, Dalbergia odorifera bark extract, Laminaria japonica extract, and Pteris vittata extract. Its possible mechanism of action is as follows: The core discovery of this invention lies in the fact that the above-mentioned components, when combined in a specific ratio, produce a significant synergistic effect. The mechanism may be manifested in: Multi-pathway coverage: Undecenoyl phenylalanine is responsible for upstream signal regulation, while various plant extracts are responsible for downstream enzyme activity inhibition and anti-oxidation, forming a full-pathway, multi-target inhibition network from signal transduction to biochemical reactions, making the inhibition effect more thorough and efficient.

[0021] Complementary action sites: Different extracts may act on different sites of tyrosinase or exert their effects through different chemical mechanisms (such as competitive inhibition and copper ion chelation). Combined use can more comprehensively block the activity of tyrosinase.

[0022] Improving the skin microenvironment: The soothing and anti-inflammatory properties of algae extracts in the compound plant extracts in the composition may help improve the skin microenvironment, reduce pigmentation caused by inflammatory factors, and create favorable conditions for other ingredients to work effectively.

[0023] In summary, the whitening composition provided by this invention is not a simple additive combination of ingredients, but rather a scientifically constructed multi-layered system of action. Through the synergistic effect of three major mechanisms—signal blocking (undecenoyl phenylalanine), direct enzyme inhibition (multiple plant extracts), and antioxidant activity (multiple plant extracts)—it precisely intervenes in the melanin synthesis pathway from different dimensions, thereby achieving a highly efficient, gentle, and comprehensive whitening effect. This mechanism-based compound design is the key to the excellent results achieved by this invention. Detailed Implementation

[0024] To better understand the present invention, the present invention will be further described below with reference to specific embodiments. The terms used in "composition" and "application example" are for describing specific implementation schemes and do not constitute a limitation on the scope of protection of the present invention.

[0025] Unless otherwise specified, experimental methods in the following examples are generally performed under standard conditions or as recommended by the manufacturer. Unless otherwise stated, all measurements are by percentage and parts by mass.

[0026] Some of the raw materials and their sources are as follows: Undecenoylphenylalanine: purchased from Shanghai Saif Chemical Development Co., Ltd.; Chickweed extract: purchased from Guangzhou Jiashang Yimei Biotechnology Co., Ltd.; Extract from the bark of the rosewood tree: purchased from Shanghai Meiyan Biotechnology Co., Ltd.; Extract of *Leptochloa crus-galli*: purchased from Guangzhou Jiashang Yimei Biotechnology Co., Ltd.; Extract of *Pteris vittata*: purchased from Guangzhou Jiashang Yimei Biotechnology Co., Ltd.; Cetearyl oleate: purchased from Shanghai Ronghai Biotechnology Co., Ltd.; Sorbitan oleic acid ester: purchased from Shanghai Ronghai Biotechnology Co., Ltd.; All other raw materials and reagents are commercially available.

[0027] The components and their mass fractions of the whitening composition are as follows: Composition 1 Composed of the following components by mass: Undecenoylphenylalanine: 0.3 parts; Emulsifier: 0.8 parts; Chickweed extract: 2 parts; Dalbergia odorifera bark extract: 4 parts; *Leptochloa crus-galli* extract: 2 parts; Pteris reticulata extract: 0.6 parts; The emulsifiers are cetearyl oleate and sorbitan oleate in a mass ratio of 3:2.

[0028] Composition 2 Composed of the following components by mass: Undecenoylphenylalanine: 0.1 parts; Emulsifier: 0.7 parts; Chickweed extract: 1 part; Dalbergia odorifera bark extract: 3 parts; *Leptochloa crus-galli* extract: 1 part; Pteris reticulata extract: 0.5 parts; The emulsifiers are cetearyl oleate and sorbitan oleate in a mass ratio of 3:2.

[0029] Composition 3 Composed of the following components by mass: Undecenoylphenylalanine: 0.5 parts; Emulsifier: 0.9 parts; Chickweed extract: 3 parts; Dalbergia odorifera bark extract: 5 parts; *Leptochloa crus-galli* extract: 3 parts; Fern-like winged algae extract: 0.7 parts.

[0030] The emulsifiers are cetearyl oleate and sorbitan oleate in a mass ratio of 3:2.

[0031] Composition ① Unlike Composition 1, this composition lacks the extract of Chickweed. The missing mass fractions are made up by extracts of Dalbergia odorifera bark, Laminaria japonica extract, and Pteridophyta fasciata extract in a mass ratio of 4:2:0.6. The remaining components and their mass fractions are the same as those in Composition 1.

[0032] Composition ② Unlike Composition 1, this composition lacks the extract of Dalbergia odorifera bark. The missing mass fractions are made up by extracts of Stellaria media, Laminaria japonica, and Pteridophyta fasciata in a mass ratio of 2:2:0.6. The remaining components and their mass fractions are the same as those in Composition 1.

[0033] Composition ③ Unlike Composition 1, this composition lacks the extract of *Leptochloa crus-galli*. The missing mass fractions are made up by extracts of *Stellaria media*, *Dalbergia odorifera* bark, and *Pteris vittata* in a mass ratio of 2:4:0.6. The remaining components and their mass fractions are the same as those in Composition 1.

[0034] Composition ④ Unlike Composition 1, it lacks the extract of *Pteris vittata*, and the missing mass fractions are made up by extracts of *Stellaria media*, *Dalbergia odorifera* bark, and *Pteris vittata* in a mass ratio of 2:4:2. The remaining components and their mass fractions are the same as those in Composition 1.

[0035] Composition ⑤ Unlike Composition 1, it lacks undecenoyl phenylalanine and emulsifier, and the missing parts by weight are made up by extracts of Stellaria media, Dalbergia odorifera bark extract, Laminaria japonica extract and Pteridophyta fasciata extract in a mass ratio of 2:4:2:0.6. The remaining components and their parts by weight are the same as those in Composition 1.

[0036] Composition ⑥ Unlike Composition 1, it lacks the extracts of Chickweed, Dalbergia odorifera bark, Laminaria japonica, and Pteridophyta fasciata. The missing components are made up by undecylenoyl phenylalanine and emulsifier in a mass ratio of 0.3:0.8. The emulsifier composition is the same as that of Composition 1, and the remaining components and their mass proportions are the same as those of Composition 1.

[0037] Composition ⑦ Undecenoylphenylalanine: 0.3 parts; Emulsifier: 0.8 parts; Chickweed extract: 0.6 parts; Dalbergia odorifera bark extract: 2 parts; *Leptochloa crus-galli* extract: 2 parts; Pteris reticulata extract: 4 parts; The emulsifier is cetearyl oleate and sorbitan oleate in a mass ratio of 3:2.

[0038] The preparation method of the above composition 1 is as follows: Weigh each component according to the above composition formula, mix them evenly, seal, and store in the dark.

[0039] The preparation methods of compositions 2-3 and ①-⑦ are the same as those of composition 1.

[0040] An essence base, its components, mass percentages, and preparation method are as follows: Glycerin: 4wt% Sodium hyaluronate: 0.1 wt% Fresh ketone: 0.5 wt% 1,2-Hexanediol: 0.5 wt%; Sclerotium sclerotium gum: 1 wt%; Add deionized water to bring the total to 100 wt%.

[0041] The preparation method of the essence base is as follows: S1: Heat deionized water, sclerotium gum, glycerin, sodium hyaluronate, and ketone to 80°C and stir until homogeneous to obtain mixture 1; S2: Cool mixture 1 to 45°C, add 1,2-hexanediol and stir until homogeneous, then continue cooling to 30°C to obtain the essence matrix.

[0042] Preparation of a whitening serum: Serum 1 Includes the following components by mass percentage: Composition 1:3wt% The serum base was replenished to 100 wt%; Preparation method of serum 1: The serum base and the composition are mixed evenly to obtain the serum.

[0043] Serum 2 Unlike serum 1, composition 2 is used to replace composition 1 with an equal mass percentage, while the remaining components, mass percentages, and preparation methods are the same as those in serum 1.

[0044] Serum 3 Unlike serum 1, composition 3 is used to replace composition 1 with an equal mass percentage, while the remaining components, mass percentages, and preparation methods are the same as those in serum 1.

[0045] Serum 4 Includes the following components by mass percentage: Composition 1: 10 wt% The serum base was replenished to 100 wt%; The preparation method for serum 4 is the same as that for serum 1.

[0046] Serum 5 Includes the following components by mass percentage: Composition 1:1wt% The serum base was replenished to 100 wt%; The preparation method for serum 5 is the same as that for serum 1.

[0047] Serum ① Unlike serum 1, composition 1 is replaced with composition ① of equal mass percentage, while the remaining components, mass percentages, and preparation methods are the same as those in serum 1.

[0048] Serum ② Unlike serum 1, composition 2 is replaced with composition 1 in the same mass percentage, while the remaining components, mass percentages, and preparation methods are the same as those in serum 1.

[0049] Serum ③ Unlike serum 1, composition 3 is used to replace composition 1 with an equal mass percentage, while the remaining components, mass percentages, and preparation methods are the same as those in serum 1.

[0050] Essence ④ Unlike serum 1, composition 4 is replaced with composition 1 in the same mass percentage, while the remaining components, mass percentages, and preparation methods are the same as those in serum 1.

[0051] Essence ⑤ Unlike serum 1, composition 5 is used to replace composition 1 with an equal mass percentage, while the remaining components, mass percentages, and preparation methods are the same as those in serum 1.

[0052] Essence ⑥ Unlike serum 1, composition 6 is replaced with composition 1 in the same mass percentage, while the remaining components, mass percentages, and preparation methods are the same as those in serum 1.

[0053] Essence ⑦ Unlike serum 1, composition 7 is replaced with composition 1 in the same mass percentage, while the remaining components, mass percentages, and preparation methods are the same as those in serum 1.

[0054] Experiment 1: Effect of the composition on tyrosinase In the biosynthesis of melanin in the skin, tyrosinase is a key enzyme that acts on tyrosine, which spontaneously undergoes a series of reactions to ultimately form melanin. Tyrosinase catalyzes the production of melanin from tyrosine in a pH 6.8 buffer solution, and its absorbance can be measured at 450 nm. Raw materials that inhibit tyrosinase activity can reduce the catalytic effect of tyrosinase, thereby lowering the absorbance. The inhibitory effect of the sample on tyrosinase activity can be assessed based on changes in absorbance. Melanin production by melanocytes in the skin is the main cause of skin darkening and pigmentation. Therefore, this experiment uses tyrosinase as a testing tool; after drug treatment, the skin-whitening effect of the samples is evaluated by measuring tyrosinase activity.

[0055] Test sample: Dilute the composition (compositions 1-3, ①-⑦) to 2 g / L with sterile deionized water to obtain the test sample, which is then tested.

[0056] Positive control group: kojic acid (100 μg / mL).

[0057] Blank control group: sterile deionized water.

[0058] Test method: Add 50 μL of test sample diluent and 20 μL of 200 U / mL tyrosinase solution to a 96-well plate, vortex to mix, and incubate at 37 °C for 10 min. Then add 130 μL of L-tyrosine solution, vortex to mix, and incubate at 37 °C for 15 min. OD is then measured. 450 The nm value is used to calculate the tyrosinase inhibition rate according to the formula.

[0059] Tyrosinase inhibition rate (%) = (1 - (T - T0) / (C - CO)) × 100% In the formula: T: Absorbance of the sample tube, i.e., the absorbance of the solution after the sample reacts with tyrosinase; T0: Sample background absorbance; C: The average of three absorbance readings from the enzyme reaction tube, i.e., the absorbance of the reaction between tyrosinase and L-tyrosine without the addition of a sample; C0: Solvent background absorbance.

[0060] The results of tyrosinase inhibition rate are shown in Table 1.

[0061] Table 1 Effect of the composition on tyrosinase Test sample Tyrosinase inhibition rate (%) Composition 1 53.2 Composition 2 50.4 Composition 3 52.9 Composition ① 41.2 Composition ② 37.5 Composition ③ 43.6 Composition ④ 45.1 Composition ⑤ 30.3 Composition ⑥ 14.5 Composition ⑦ 47.3 Positive control group 95.7 Blank control group / Note: " / " indicates no inhibitory effect.

[0062] As shown in Table 1, comparing the results of compositions 1-3 and ①-⑦ with the blank control group, the compositions exhibit significant tyrosinase inhibition rates. Comparing compositions 1-3 with composition ⑦, it is evident that compositions obtained with different mass ratios of undecenoyl phenylalanine, chickweed extract, *Dalbergia odorifera* bark extract, *Leptochloa crus-galli* extract, and *Dryopteris fasciatus* extract achieve different levels of tyrosinase activity inhibition. The compositions obtained using undecenoyl phenylalanine, chickweed extract, *Dalbergia odorifera* bark extract, *Leptochloa crus-galli* extract, and *Dryopteris fasciatus* extract within the mass range specified in this invention yielded… The obtained composition showed a better inhibition rate against tyrosinase. Comparing composition 1 with compositions ①-④, it can be seen that there is a significant synergistic effect between the extracts of *Stellaria media*, *Dalbergia odorifera* bark, *Leptochloa crus-galli*, and *Pteridophora spp.*, and their combined use can significantly enhance the inhibitory effect of the composition on tyrosinase. Comparing composition 1 with compositions ⑤-⑥, it can be seen that there is a significant synergistic effect between undecenoyl phenylalanine and the extracts of *Stellaria media*, *Dalbergia odorifera* bark, *Leptochloa crus-galli*, and *Pteridophora spp.*, and their combined use can significantly enhance the inhibitory effect of the composition on tyrosinase.

[0063] Experiment 2: Effect of the composition on hyaluronidase activity Samples to be tested: Compositions 1-3, ①-⑦; Preparation of the test sample: The test sample was prepared into a 3wt% mixture using distilled water.

[0064] Reagent preparation: Hyaluronidase solution: concentration 500 U / mL, freshly prepared and used, using acetate buffer as solvent; Sodium hyaluronate solution: concentration 0.5 mg / mL, using acetate buffer as solvent; Acetic acid buffer: Mix 4.8 mL of 0.2 mol / L acetic acid and 45.2 mL of 0.2 mol / L sodium acetate, dilute to 100 mL, and prepare an acetate buffer solution with pH=5.6; Acetylacetone solution: Mix 50 mL of 1.0 mol / L sodium carbonate solution and 3.5 mL of acetylacetone thoroughly (prepare fresh before use); P-DAB reagent: 0.8 g of p-dimethylaminobenzaldehyde is dissolved in a mixture of 15 mL concentrated hydrochloric acid and 15 mL anhydrous ethanol, and mixed thoroughly. The mass concentration of the concentrated hydrochloric acid is 37%. CaCl2 solution: concentration 2.5 mol / L; NaOH solution: concentration 5 mol / L.

[0065] (1) Take four clean test tubes and label them A, B, C and D respectively. Add 50 μL of the sample to be tested to each of test tubes A and B, add 50 μL of distilled water to each of test tubes C and D, add 50 μL of 500 U / mL hyaluronidase solution to each of test tubes A and C, and add 50 μL of pH=5.6 acetate buffer to each of test tubes B and D. Place the test tubes in a 37 ℃ incubator for 20 min.

[0066] (2) Add 10 μL of 2.5 mol / L CaCl2 solution to each of the four test tubes, and then place them in a 37 ℃ incubator for 20 min.

[0067] Add 10 μL of solution and then incubate at 37 ℃ for 20 min.

[0068] (3) Add 50 μL of 0.5 mg / mL sodium hyaluronate solution to test tubes A and C, and add 50 μL of pH 5.6 acetate buffer to test tubes B and D. Place the test tubes in a 37 ℃ incubator for 40 min, and then take them out and let them stand at room temperature for 10 min.

[0069] (4) Add 50 μL of distilled water, 10 μL of 5 mol / L NaOH solution and 50 μL of acetylacetone solution to the four test tubes respectively. Place the test tubes in a water bath for 30 min of boiling water, then in an ice bath for 10 min, and finally at room temperature for 10 min.

[0070] (5) Add 100 μL of P-DAB reagent to each test tube, and then measure the absorbance at 530 nm using an enzyme-linked immunosorbent assay (ELISA) reader. Hyaluronidase inhibition rate / % = ; In the formula: A is the OD value of the solution in test tube A; B is the OD value of test tube B; C is the OD value of the solution in test tube C; and D is the OD value of the solution in test tube D.

[0071] Table 2 Effect of the composition on hyaluronidase activity Group Hyaluronidase inhibition rate / % Composition 1 63.2 Composition 2 62.5 Composition 3 62.3 Composition ① 40.3 Composition ② 39.6 Composition ③ 44.2 Composition ④ 43.5 Composition ⑤ 33.7 Composition ⑥ 7.4 Composition ⑦ 50.6 According to the results of compositions 1-3 and 1-7 in Table 2, the compositions defined in this invention have a certain inhibitory effect on hyaluronidase, indicating that they have a certain soothing effect. Specifically, comparing the results of composition 1 with those of compositions 1-4, it can be seen that when the five components defined in the compositions of this invention are used together in the mass proportions defined in this invention, the inhibitory effect on hyaluronidase is greatly enhanced, indicating that there is a significant synergistic effect among the four components in the compositions of this invention. Comparing the results of composition 1 with those of compositions 5-6, it can be seen that the undecenoyl phenylalanine provided by this invention... Composition ⑥ does not show significant inhibitory effect on hyaluronidase. The extracts of Stellaria media, Dalbergia odorifera bark, Laminaria japonica, and Pteridophytum comosum (Composition ⑤) have certain inhibitory effects on hyaluronidase. However, when the two are used in combination in the mass fractions specified in this invention (Composition 1), the inhibition rate of hyaluronidase is higher, indicating that there is a significant synergistic effect between the two. Comparing the results of Compositions 1-3 with Composition ⑦, it can be seen that when the components in the compositions specified in this invention are used in combination within the mass fraction range specified in this invention, the inhibitory effect on hyaluronidase activity is better.

[0072] Experiment 3: Human Efficacy Test 3-1: Serum Safety Test The irritation of cosmetics was evaluated using the 2015 Cosmetic Safety Technical Specifications as a reference standard. The test method was a skin patch test, and the test was conducted on volunteers aged 16-65 years who were randomly distributed. Each volunteer was assigned to all test products, for a total of 30 volunteers.

[0073] Test substances: serums 1-5, serums ①-④, ⑦, serum base.

[0074] Test Method: The volunteer's back was divided into 13 test areas using tape. The test substance was placed in a patch applicator (0.020-0.025g). The patch applicator containing the test substance was then covered with non-irritating cloth-based adhesive tape over the test area. One sample was used for each test area. The patch was gently pressed with the palm of the hand to ensure even adhesion to the skin surface. The application was left on for 24 hours. The patch applicator was removed 30 minutes later, and the skin reaction was observed after the pressure marks disappeared. If the result was negative, the results were repeated at 24 and 48 hours post-pattern testing.

[0075] Evaluation criteria: Grade 0: Negative reaction; Grade 1: Suspicious reaction, with only slight erythema; Grade 2: Weak positive reaction, erythema, infiltration, edema, and possible papules; Grade 3: Strong positive reaction, erythema, infiltration, edema, papules may be present, and the reaction may extend beyond the test area; Grade 4: Extremely positive reaction, with obvious erythema, severe infiltration, edema, confluent herpes, and reaction extending beyond the test area.

[0076] Test results: All subjects had negative skin reactions.

[0077] 3-2 Human Efficacy Test Test samples: serums 1-5, serums ①-④, ⑦, serum base.

[0078] Test area: Full face.

[0079] Methods: Volunteers aged 30-50 years with skin whitening needs were selected as subjects and randomly assigned to one test sample per group, with 10 subjects per group. During the 28-day trial, subjects used the test sample once a day, with each application consisting of 3 mL.

[0080] Data Collection: A skin red-melanin analyzer was used to assess the changes in skin melanin content in the test areas of the subjects before and after the application of the test sample. The measured values ​​were directly proportional to the melanin content. Before applying the test sample, the melanin content in the test area was measured and recorded as M0. On the first day after the experiment ended, i.e., day 29, the melanin content in the test area was measured and recorded as M1. The rate of change in melanin content X / % was calculated as (M0-M1) / M0×100%, and the results are shown in Table 3.

[0081] Table 3 Human Efficacy Test Test sample Melanin content change rate X / % Serum 1 30.4 Serum 2 27.6 Serum 3 29.3 Serum 4 33.6 Serum 5 23.5 Serum ① 22.6 Serum ② 19.7 Serum ③ 23.3 Essence ④ 23.9 Essence ⑦ 24.2 serum base -0.03 According to the results in Table 3, comparing serums 1-5 with the serum matrix, it is clear that the serum matrix in this invention does not affect the rate of change in melanin content. The reason why the serum can improve the melanin content in the skin is due to the addition of the composition. Comparing serums 1-3 with serum ⑦, it is evident that the whitening effects achieved by compositions with different mass ratios of undecenoyl phenylalanine, chickweed extract, rosewood bark extract, *Paederia scandens* extract, and *Pteris vittata* extract are different. Using undecenoyl phenylalanine within the mass range of this invention... The composition obtained from undecenoyl phenylalanine, chickweed extract, rosewood bark extract, *Leptochloa crus-galli* extract, and *Pteris vittata* extract can more effectively reduce the melanin content in the skin. A comparison of serum 1 and serums 4-5 shows that the dosage of the composition specified in this invention is more cost-effective. A comparison of serum 1 and serums ①-④ shows that the chickweed extract, rosewood bark extract, *Leptochloa crus-galli* extract, and *Pteris vittata* extract in the composition have a significant synergistic effect, and their combined use can significantly enhance the whitening effect of the serum.

[0082] The embodiments described above are some, but not all, of the embodiments of this application. The detailed description of the embodiments of this application is not intended to limit the scope of the claimed application, but merely to illustrate selected embodiments. All other embodiments obtained by those skilled in the art based on the embodiments of this application without inventive effort are within the scope of protection of this application.

Claims

1. A whitening composition, characterized in that, The composition comprises the following components in parts by weight: Undecenoyl phenylalanine: 0.1-0.5 parts; Emulsifier: 0.7-0.9 parts; Chickweed extract: 1-3 parts; Dalbergia odorifera bark extract: 3-5 parts; Laminaria japonica extract: 1-3 parts; Pteris reticulata extract: 0.5-0.7 parts; The emulsifier is at least one of cetearyl oleate, sorbitan oleate, and glyceryl stearate.

2. The composition according to claim 1, characterized in that, The composition comprises the following components in parts by weight: Undecenoylphenylalanine: 0.3 parts; Emulsifier: 0.8 parts; Chickweed extract: 2 parts; Dalbergia odorifera bark extract: 4 parts; *Leptochloa crus-galli* extract: 2 parts; Pteris reticulata extract: 0.6 parts; The emulsifier is cetearyl oleate and sorbitan oleate in a mass ratio of 3:

2.

3. The use of the composition as described in claim 1 or 2 in the preparation of cosmetics with whitening effects.

4. The application as described in claim 3, characterized in that, The dosage form of the cosmetic includes any one of lotion, serum, cream, mask, and spray.

5. A whitening serum, characterized in that, The serum comprises the whitening composition as described in claim 1 or 2.

6. The essence as described in claim 5, characterized in that, The serum also includes skin conditioning agents, preservatives, thickeners, and solvents.

7. The essence as described in claim 6, characterized in that, The skin conditioning agent includes at least one of glycerin, hyaluronic acid, sodium hyaluronate, and panthenol.

8. The essence as described in claim 6, characterized in that, The preservatives include at least one of phenoxyethanol, phenoxyethanol, potassium sorbate, and 1,2-hexanediol.

9. The essence as described in claim 6, characterized in that, The thickener includes at least one of gellan gum, sodium alginate, carbomer, xanthan gum, and sclerotinia gum.

10. The essence as described in claim 6, characterized in that, The solvent is deionized water.