Bacillus coagulans and applications thereof

Abstract

The application provides a bacillus coagulans (Bacillus coagulans) Bacillus coagulans ) and application thereof. The bacillus coagulans of the application is preserved in the China General Microbiological Culture Collection Center, and has a preservation number of CGMCC No. 36786. The bacillus coagulans of the application has multiple antimicrobial lipopeptide genes and ACC deaminase genes; has strong stress resistance, high fermentation activity, and the ability to produce antimicrobial lipopeptides and secrete plant growth hormones; can effectively prevent and control various crop diseases, and can also directly promote the growth and development of crops, improve the stress resistance of crops, and promote the yield increase of crops.

Description

Technical Field

[0001] This invention relates to the field of bioengineering technology, and in particular to a Bacillus coagulans and its applications. Background Technology

[0002] Plant growth-promoting bacteria (PGPBs) are a class of microorganisms that play a crucial role in plant growth, health, and environmental remediation. As early as the beginning of the 20th century, scientists isolated strains of Bacillus and other organisms with antagonistic abilities against pathogens from soil, initiating empirical research. The 1950s to 1980s were the "golden foundation period" for understanding their mechanisms, revealing how they directly inhibit bacteria through antibiotic secretion and nutrient competition, and discovering the mechanism of "inducing systemic resistance"—activating the plant's own immunity. The concept of "plant growth-promoting bacteria" was proposed in 1980, establishing this research field. In the 1990s, the first generation of commercial products appeared, but inconsistent field results highlighted the complexity of the environment. Entering the 21st century, research entered the era of multi-omics and ecology; genome sequencing revealed the functional blueprint of strains, and the research perspective expanded from single strains to the entire root microbiome. The current cutting edge is to construct "synthetic microbial communities" and use synthetic biology to design strains with stronger functions. This research has achieved a leap from phenomenon to mechanism, from single to system, and from blind screening to precise design, providing a solid scientific and technological foundation for green agriculture.

[0003] In recent years, research on plant growth-promoting bacteria has made groundbreaking progress in mechanism analysis, technological application, and research concepts. At the mechanistic level, research has moved beyond early descriptions of single functions such as growth promotion and antibacterial activity to delve into systemic interaction networks. The focus has shifted to how these bacteria "activate" plant systemic resistance through signaling molecules and how they reshape the rhizosphere microecology, indirectly protecting plant health by regulating microbial community structure. The integration of genomics, transcriptomics, and metabolomics allows for a systematic analysis of their functional blueprint and dynamic effects. More importantly, gene editing technology has made it possible to precisely enhance functional genes, driving the creation of highly efficient engineered strains. To overcome the bottleneck of unstable field effects of single strains, cutting-edge research has shifted to synthetic microbial communities. This involves rationally combining strains with different functions (such as nitrogen fixation and biocontrol) to construct a collaborative "microbial community team," achieving more stable and powerful field efficacy. In summary, research on growth-promoting bacteria is moving from "discovery" to "design," providing a core driving force for achieving green and sustainable agriculture through precise microscopic manipulation.

[0004] Currently, growth-promoting bacteria have become an important component of green agriculture, with their market application booming, forming a diversified product system, and demonstrating significant benefits in practice. In terms of practical application effects, high-quality growth-promoting bacteria products can bring multiple benefits, such as increasing crop yield and improving fruit quality; preventing diseases and ensuring crop health; reducing the use of chemical fertilizers and pesticides, lowering usage costs, enhancing crop drought and salt tolerance, and improving soil health. The mainstream products in the market are mainly divided into three categories: 1. Biofertilizers / stimulants, with nitrogen-fixing, phosphorus-solubilizing, and potassium-solubilizing bacteria (such as rhizobia and Bacillus mucilaginosus) as the core. They convert ineffective nutrients in the environment into forms that plants can absorb through microbial metabolism, directly reducing reliance on chemical fertilizers. 2. Biopesticides / biocontrol agents, with Bacillus subtilis and Bacillus amyloliquefaciens as star strains. They effectively prevent and inhibit soil-borne and foliar diseases through mechanisms such as competition, antagonism, and induction of plant resistance, becoming a key tool for reducing chemical pesticide use. 3. Compound functional microbial agents: Representing the future trend, these agents scientifically combine multiple functional strains (such as growth promoters, disease preventers, and stress-resistant agents) to form a synergistic "microbial community team," providing a one-stop solution with more stable and comprehensive effects.

[0005] Microbial inoculants face numerous challenges and shortcomings, primarily in maintaining their activity. From production and storage to field application, microbial inoculants are extremely sensitive to parameters such as temperature, pH, dissolved oxygen, and stirring rate during fermentation. Some strains exhibit slow growth, low bacterial counts, and low activity, failing to meet application requirements. Furthermore, live bacteria are easily inactivated by environmental factors such as high temperature, high humidity, and salinity. After application to the soil, exogenous bacteria face "acclimatization problems," struggling to colonize and compete in the complex microecology, leading to unstable and poor efficacy. Therefore, to address these challenges, it is necessary to select strains with strong stress resistance and advanced functions. Fermenting highly active strains can improve fermentation efficiency, reduce costs, and ensure stable product activity and quality. These strains should also possess multiple functions, promoting plant growth and disease resistance, such as producing antimicrobial lipopeptides and secreting plant growth hormones, thereby promoting soil health and maintaining the soil-microbe-plant interaction.

[0006] In view of this, the present invention is proposed. Summary of the Invention

[0007] The purpose of this invention is to provide a Bacillus coagulans and its application, which can be used to promote crop yield and prevent diseases.

[0008] In a first aspect, the present invention provides a Bacillus coagulans strain named GN8, wherein the Bacillus coagulans strain is classified and named... Bacillus coagulans It is deposited at the China General Microbiological Culture Collection Center, with accession number CGMCC No. 36786.

[0009] The Bacillus coagulans of the present invention ( Bacillus coagulans The strain was screened from tomato rhizosphere soil samples and named Bacillus coagulans GN8. It was deposited on November 27, 2025, at the China General Microbiological Culture Collection Center (CGMCC) with accession number CGMCC No. 36786 and address: No. 3, No. 1 Beichen West Road, Chaoyang District, Beijing.

[0010] The *Bacillus coagulans* GN8 described in this invention exhibits a wavy, round, milky-yellow, opaque colony morphology on LB agar plates, with a rough, dry, and dull surface and irregular edges. Microscopic observation under an oil immersion microscope (oil 100×10) reveals that the bacterial cells are short rods, and after Gram staining, they appear purple, confirming the bacteria as Gram-positive.

[0011] Specifically, the nucleotide sequence of the 16S rDNA of Bacillus coagulans GN8 is shown in SEQ ID NO:1.

[0012] Specifically, the Bacillus coagulans possesses multiple antimicrobial lipopeptide genes and ACC deaminase genes.

[0013] Specifically, the multiple antimicrobial lipopeptide genes include sfp, lpa-14, ituD, and fenB, and the ACC deaminase gene is acdS.

[0014] Specifically, the Bacillus coagulans has strong stress resistance, high fermentation activity, and the ability to produce antimicrobial lipopeptides and secrete plant growth hormones.

[0015] Specifically, the Bacillus coagulans has the effect of promoting crop yield and preventing diseases.

[0016] Preferably, the crops include one or more of the following: tomatoes, strawberries, cucumbers, chili peppers, peppers, celery, cauliflower, zucchini, Atractylodes lancea, citrus fruits, corn, peanuts, ginger, rice, potatoes, and sunflowers.

[0017] Preferably, the diseases include one or more of the following: bacterial wilt of tomato, wilt of tomato, damping-off of tomato, late blight of tomato, gray mold of tomato, anthracnose of chili pepper, anthracnose of pepper, root rot of celery, stem base rot of celery, downy mildew of cauliflower, bacterial soft rot of cauliflower, black spot of cauliflower, powdery mildew of zucchini, root rot of Atractylodes lancea, citrus canker, large leaf spot of corn, small spot disease of corn, and rust of corn.

[0018] Specifically, the Bacillus coagulans has antagonistic ability against Solanaceae Raulella, Fusarium oxysporum, and Rhizoctonia solani.

[0019] Preferably, the fermentation method of the Bacillus coagulans is as follows: Bacillus coagulans GN8 is inoculated into LB liquid medium, cultured at 28 ℃ and 180 r / min for 24 h to prepare fermentation seed liquid, and fermentation expansion is carried out using a DME culture machine.

[0020] A second aspect of this invention provides a microbial agent containing the aforementioned Bacillus coagulans.

[0021] Preferably, the microbial agent uses Bacillus coagulans as the main microorganism.

[0022] Preferably, the microbial agent is a solid agent or a liquid agent.

[0023] Preferably, the microbial agent contains live cells of the Bacillus coagulans strain, freeze-dried dried cells of the Bacillus coagulans strain, immobilized cells of the Bacillus coagulans strain, or the Bacillus coagulans strain existing in any other form.

[0024] Preferably, the microbial agent is obtained by expanding the seed culture of Bacillus coagulans.

[0025] A third aspect of this invention provides the application of the aforementioned Bacillus coagulans or the aforementioned microbial agent in promoting crop yield.

[0026] Preferably, the crops include one or more of the following: tomatoes, strawberries, cucumbers, chili peppers, peppers, celery, cauliflower, zucchini, Atractylodes lancea, citrus fruits, corn, peanuts, ginger, rice, potatoes, and sunflowers.

[0027] The fourth aspect of this invention provides the application of the aforementioned Bacillus coagulans or the aforementioned microbial agent in the prevention and control of crop diseases.

[0028] Preferably, the diseases include one or more of the following: bacterial wilt of tomato, wilt of tomato, damping-off of tomato, late blight of tomato, gray mold of tomato, anthracnose of chili pepper, anthracnose of pepper, root rot of celery, stem base rot of celery, downy mildew of cauliflower, bacterial soft rot of cauliflower, black spot of cauliflower, powdery mildew of zucchini, root rot of Atractylodes lancea, citrus canker, large leaf spot of corn, small spot disease of corn, and rust of corn.

[0029] Compared with the prior art, the present invention has at least the following beneficial effects: (1) The Bacillus coagulans of the present invention can secrete a variety of antimicrobial lipopeptides and plant growth factors, such as: lipopeptide compounds Surfactins, Iturins, Fengycins, plant growth factor IAA, and stress resistance factor ACC deaminase, which can effectively prevent and control a variety of crop diseases, and can also directly promote the growth and development of crops, reduce ethylene content, improve the stress resistance of crops, and promote crop yield.

[0030] (2) The Bacillus coagulans bacterial solution after fermentation and expansion of the present invention has a strong ability to inhibit crop diseases and promote crop growth. For example, the control efficacy against three tomato diseases is greater than 60%, and the increase in plant height, stem diameter, fresh weight and chlorophyll content of tomato seedlings is 37.0%, 18.4%, 116.2% and 24.13%, respectively.

[0031] (3) Bacillus coagulans can form spores and is highly resistant to adverse environments such as dryness, oxidation, and ultraviolet light in a dormant state, making it easy to preserve and transport for a long time. It can also survive for a short time in high-temperature environments, which helps it avoid losing high activity during formulation. Its facultative anaerobic nature also allows it to be used under various fermentation and application conditions. It can grow, reproduce, and function normally in both anaerobic and aerobic environments. As an animal intestinal microorganism, it can grow in a strongly acidic environment and secrete a variety of antibacterial substances such as bacteriocins and coagulants, which can directly inhibit or kill pathogenic microorganisms.

[0032] (4) Bacillus coagulans has a mature production and fermentation process. With the advancement of fermentation technology and post-processing technology, the production cost has gradually decreased, and the number of live bacteria and stability have been continuously improved. Its microbial preparations are environmentally friendly as green and pollution-free bio-fertilizers.

[0033] Based on the characteristics and process of the above-mentioned strains, the Bacillus coagulans of the present invention can be used directly as a microbial preparation. Attached Figure Description

[0034] To more clearly illustrate the specific embodiments of the present invention or the technical solutions in the prior art, the drawings used in the description of the specific embodiments or the prior art will be briefly introduced below. Obviously, the drawings described below are some embodiments of the present invention. For those skilled in the art, other drawings can be obtained from these drawings without creative effort.

[0035] Figure 1 This is a colony morphology diagram of Bacillus coagulans GN8 provided by the present invention.

[0036] Figure 2 The image shows the microscopic morphology of Bacillus coagulans GN8 provided by this invention.

[0037] Figure 3 Phylogenetic tree of the 16S rDNA gene of Bacillus coagulans GN8 provided by the present invention.

[0038] Figure 4 The image shows the antagonistic plate diagrams of Bacillus coagulans GN8 against Raulella solanaceae, Fusarium oxysporum, and Rhizoctonia solani provided by this invention; from left to right, these are the antagonistic plates of GN8 against Raulella solanaceae, Fusarium oxysporum, and Rhizoctonia solani.

[0039] Figure 5 The image shows the detection results of the surfactant production characteristics of Bacillus coagulans GN8 provided by this invention.

[0040] Figure 6 The image shows the detection results of IAA production characteristics of Bacillus coagulans GN8 provided by the present invention; the left is the blank control, and the right is GN8 bacterial solution with Salkowski colorimetric solution.

[0041] Figure 7 The following figures illustrate the control results of Bacillus coagulans GN8 against tomato diseases provided by this invention; from left to right, they show the control results of GN8 against Solanaceae Raulella, Fusarium oxysporum, and Rhizoctonia solani; in each figure, left: only pathogens were inoculated, right: GN8 inoculated with both bacterial solution and pathogens.

[0042] Figure 8 The figure shows the growth-promoting effect of Bacillus coagulans GN8 on tomatoes provided by this invention. Detailed Implementation

[0043] It should be noted that the following detailed descriptions are illustrative and intended to provide further explanation of this application. Unless otherwise specified, all technical and scientific terms used herein have the same meaning as commonly understood by one of ordinary skill in the art to which this application pertains.

[0044] It should be noted that the terminology used herein is for the purpose of describing particular embodiments only and is not intended to limit the exemplary embodiments according to this application. As used herein, the singular form includes the plural form unless the context clearly indicates otherwise. Furthermore, it should be understood that when the terms "comprising" and / or "including" are used in this specification, they indicate the presence of features, steps, operations, devices, components, and / or combinations thereof.

[0045] The technical solution of the present invention will be clearly and completely described below with reference to the embodiments. Obviously, the described embodiments are only some embodiments of the present invention, and not all embodiments. Based on the embodiments of the present invention, all other embodiments obtained by those skilled in the art without creative effort are within the scope of protection of the present invention.

[0046] Example 1 Isolation of strains Soil samples were collected from the rhizosphere of tomatoes at a depth of 5-15 cm using the five-point method. Each soil sample was then diluted 10-fold using the plate dilution method to prepare 10 separate samples. -4 ~10 -6 For serial dilution, take 100 μL of the dilution and spread it on LB medium. Incubate at 37°C for 24 h. Pick colonies with different morphological and color characteristics for purification. Transfer the purified single colonies to LB slant and store at 4°C for later use.

[0047] Example 2: Identification and classification of strain species (1) Morphological identification Morphological identification of strain GN8: Observe the colony morphology of strain GN8 on LB agar plates by streaking. The colonies of strain GN8 are all wavy and round, milky yellow, opaque, with a rough, dry, and dull surface, and irregular edges. Figure 1 ).

[0048] The morphology of strain GN8 was observed under an oil immersion microscope (oil 100×10). The bacterial cells were short rod-shaped and turned purple after Gram staining, indicating that it was identified as a Gram-positive bacterium. Figure 2 ).

[0049] (2) Molecular biological identification (phylogenetic tree of 16S rDNA gene) Sequence analysis of the 16S rDNA gene (SEQ ID NO:1) of strain GN8 was performed, and homology alignment (Blastn) was performed with the nucleic acid sequence in GenBank. The strain was preliminarily identified as Bacillus coagulans. Bacillus coagulans () Figure 3 ).

[0050]

[0051] Example 3: Detection of the ability of bacterial strains to antagonize pathogens The antagonistic ability of bacterial strains against *Raulella solani*, *Fusarium oxysporum*, and *Rhizoctonia solani* was tested using the filter paper disc method and the plate confrontation method. Figure 4 The results showed that Bacillus coagulans GN8 had a clear gap between itself and Solanaceae Raulella, Fusarium oxysporum, and Rhizoctonia solani. Its inhibition rates against these pathogens were 49.01%, 62.15%, and 63.14%, respectively, which effectively inhibited the growth of these pathogens.

[0052] This shows that strain GN8 has a strong ability to resist pathogens, including bacterial wilt, Fusarium wilt, and damping-off of tomatoes, indicating that strain GN8 has good potential for disease prevention and suppression.

[0053] Example 4: Detection of the ability of strains to produce antimicrobial lipopeptides and secrete phytokinins Strain GN8 has the ability to produce antimicrobial lipopeptides and secrete plant growth hormones. This ability can be demonstrated by the surfactant assay and the 3-indoleacetic acid (IAA) colorimetric assay, which is beneficial for promoting plant growth.

[0054] (1) Detection method for surfactant production (oil ring method) The selected GN8 strain was inoculated into LB liquid medium and cultured at 28 °C and 180 r / min on a shaker for 48 h. The cells were removed by centrifugation at 10000 r / min for 15 min, and the fermentation supernatant was filtered through a 0.22 μm pore size filter for sterilization. A suitable amount of sterile water was added to a petri dish, followed by 100 μL of liquid paraffin to form an oil film on the water surface. Then, 10 μL of fermentation supernatant was added to the center of the oil film. The central oil film was pushed outwards to form a circle, the size of which was directly proportional to the surfactant content. Each treatment was repeated three times. The results are shown in the figure below. Figure 5 The average diameter of the oil ring formed by the fermentation supernatant of strain GN8 in the oil removal experiment was 6.2 cm, indicating that strain GN8 can secrete a large number of surface-active metabolites under fermentation conditions.

[0055] (2) Detection method for IAA (Salkowski colorimetric method) The selected GN8 strain was inoculated into LB liquid medium containing L-tryptophan (100 mg / L) and cultured at 28°C and 180 rpm for 24 h. Then, 50 μL of the supernatant was dropped onto a white ceramic plate, and an equal volume of Salkowski's colorimetric solution was added and mixed thoroughly. The white ceramic plate was then placed at room temperature in the dark for 30 min. Results are shown below. Figure 6Using uninoculated LB liquid medium as a blank control, the solution inoculated with GN8 bacteria turned red, indicating that the GN8 strain has the ability to produce IAA.

[0056] This shows that strain GN8 has the ability to produce surfactants and plant growth hormones, which can prevent disease infection and promote plant growth and development.

[0057] Example 5: Detection of multiple antimicrobial lipopeptide genes and ACC deaminase genes in strains The coding and regulatory genes of three important families of antimicrobial lipopeptides—surfactin, iturin, and fengycin—were selected. sfp, lpa-14, ituD, fenB ), ACC deaminase structural gene acdS PCR amplification was performed on the target gene. Primers were designed based on the homologous genes in GenBank and synthesized by Beijing Qingke Biotechnology Co., Ltd. The primer sequences are shown in Table 1. Total DNA was extracted from each strain using a DNA extraction kit (Beijing Kangrun Chengye Biotechnology Co., Ltd.). PCR was performed in a 25 μL system, using the strain's DNA genome as a template. Reaction conditions: amplification... sfp , LPA-14 and acdS The following conditions were met: 95 °C for 2 min; 95 °C for 15 s, 72 °C for 15 s, 72 °C for 12 s, 30 cycles; 72 °C for 5 min; detection. ituD The following conditions were met: 95 °C for 2 min; 95 °C for 15 s, 72 °C for 15 s, 72 °C for 19 s, 30 cycles; 72 °C for 5 min; detection. fenB The cycles were: 95 °C, 2 min; 95 °C, 15 s, 72 °C, 15 s, 72 °C, 27 s, 32 cycles; 72 °C, 5 min. After identification of the PCR products by agarose gel electrophoresis, the target fragment was recovered and ligated into the cloning vector pMD19-T, transformed into E. coli DH5α, and plasmids were extracted from several randomly selected positive transformants. Sequencing was performed by Beijing Qingke Biotechnology Co., Ltd.

[0058] Table 1 GN8 Amplification sfp , LPA-14 , ituD , fenB and acdS Gene similarity

[0059] Example 6: Fermentation Capacity Test of Strains The selected strain GN8 was transferred to LB liquid medium and cultured at 28 ℃ with shaking at 180 r / min for 24 h to prepare a fermentation seed culture. Fermentation expansion was then carried out using a DME culture machine. The culture was prepared at a rate of 5 × 10⁻⁶... 7 Add an appropriate amount of seed culture (cfu / mL) to the inoculation chamber, add Bacillus aeruginosa culture medium to the fermenter, and bring the volume to 25 L with water. Start the equipment and run it automatically for 23 hours. After fermentation, perform Gram staining and microscopic examination of the fermentation broth, and determine the bacterial count using a serial dilution method.

[0060] The GN8 fermentation cell count was measured to be 3.2 × 10⁻⁶. 9 cfu / mL, contamination rate less than 0.5%, Gram positive, rod-shaped, no spores formed, single cell size approximately (0.7~1.0) μm × (2.2 × 3.1) μm.

[0061] Experimental Example 1: Control of Tomato Diseases by Strain GN8 The effects of the strain on disease control in tomato seedlings were verified using a nutrient soil culture system.

[0062] Tomato seeds: Soak in 95% alcohol for 1 min, soak in 2.5% sodium hypochlorite for 3 min, and rinse with sterile water 4-5 times. Place the soaked seeds on a moistened gauze, put them in a petri dish, and incubate at 25 ℃ for 3 days.

[0063] Experimental strain: GN8, LB liquid medium, 28℃, 180rpm, cultured for 24 h; the bacterial solution was centrifuged at 8000 rpm for 10 min to obtain bacterial sludge, washed once with physiological saline and then resuspended, OD600 was adjusted to 0.5, and 2 mL / strain was inoculated.

[0064] Pathogen inoculation: Inoculate the roots of tomato seedlings when they have 1-2 true leaves. Dilute *Raylordia solani* to a concentration of 1×10⁻⁶. 8 CFU / mL, inoculate 0.5 mL per tomato seedling; cut Fusarium oxysporum and Rhizoctonia solani culture medium into 1 cm square pieces, and place 3 pieces at the base of the tomato seedlings; one day after inoculation with pathogens, inoculate with the fermentation broth of the test strain, diluted to 5 × 10⁻⁶. 8 CFU / mL, 0.5 mL per tomato seedling was inoculated at the base of the seedling; plants not inoculated with GN8 bacterial solution served as a blank control.

[0065] After treatment, the samples were placed in an artificial climate chamber at 25°C and 70% humidity, with 16 hours of light followed by 8 hours of darkness, and appropriate water was added every two days. After 25-30 days of cultivation, the disease incidence index was measured. Results are shown below. Figure 7As shown in Table 2, strain GN8 exhibits a control efficacy of over 60% against all three tomato diseases, indicating that strain GN8 has a significant control effect on tomato diseases.

[0066] Table 2. Disease control efficacy of GN8 bacterial inoculation treatment for various diseases.

[0067] Experimental Example 2: Strain GN8 promotes the growth of tomato seedlings The effects of the bacterial strain on the growth of tomato seedlings were verified using a nutrient soil culture system.

[0068] Tomato seeds: Soak in 95% alcohol for 1 min, soak in 2.5% sodium hypochlorite for 3 min, and rinse with sterile water 4-5 times. Place the soaked seeds on a moistened gauze, put them in a petri dish, and incubate at 25 ℃ for 3 days.

[0069] Experimental strain: GN8, LB liquid medium, 28℃, 180rpm, cultured for 24 h; the bacterial solution was centrifuged at 8000 rpm for 10 min to obtain bacterial sludge, washed once with physiological saline and then resuspended, OD600 was adjusted to 0.5, and 2 mL / strain was inoculated.

[0070] After treatment, the seedlings were placed in an artificial climate chamber at 25°C and 70% humidity, with 16 hours of light followed by 8 hours of darkness, and watered appropriately every two days. The seedlings were cultured for 25-30 days, and their growth was then assessed. Results are shown below. Figure 8 According to Table 3, strain GN8 increased the plant height, stem diameter, fresh weight and chlorophyll content of tomato seedlings by 37.0%, 18.4%, 116.2% and 24.13%, respectively, indicating that strain GN8 can significantly promote the growth of tomato seedlings.

[0071] Table 3 Comparison of GN8 inoculated and uninoculated treatments

[0072] Experimental Example 3: Strain GN8 promotes tomato yield increase This experiment further verified the actual yield-increasing effect of GN8 on tomatoes. The effects of GN8 on the biological traits of tomatoes after application of the fermentation liquid are shown in Table 4: the marketable fruit weight per tomato plant after GN8 treatment was 1.85 kg, significantly higher than the 1.76 kg in the control group. Yield and benefit analysis are shown in Table 5: compared with the control group, the yield increased by 276.5 kg per mu (approximately 0.067 hectares), with a yield increase rate of 5.11%. Based on the average market price of tomatoes at 0.52 yuan / kg, the income increase per mu was 143.8 yuan, with an input-output ratio of 3.99. Therefore, GN8, through on-site fermentation and application, can maximize the protection of the inoculum activity and metabolites, thereby ensuring increased tomato yield and income.

[0073] Table 4 Comparison of biological traits of tomatoes

[0074] Table 5 Output and Benefit Analysis

[0075] Experimental Example 4: Strain GN8 promotes increased yields of cash crops and food crops Using a "functional strain-on-site fermentation-live bacteria application" technology model, GN8 was screened in multiple regions across the country, covering a wide range of soil types, and field trials were conducted on various cash crops and food crops. The results are shown in Table 6: cash crops saw yield increases of 4%–38% and a decrease in disease incidence of 2%–62%; food crops saw yield increases of 5%–25% and a decrease in disease incidence of 3%–22%. Field trials fully demonstrate that GN8 plays a positive and significant role in promoting crop yield and controlling diseases.

[0076] Table 6 Analysis of Crop Yield Increase and Disease Incidence

[0077] Finally, it should be noted that the above embodiments are only used to illustrate the technical solutions of the present invention, and not to limit them; although the present invention has been described in detail with reference to the foregoing embodiments, those skilled in the art should understand that modifications can still be made to the technical solutions described in the foregoing embodiments, or equivalent substitutions can be made to some or all of the technical features; and these modifications or substitutions do not cause the essence of the corresponding technical solutions to deviate from the scope of the technical solutions of the embodiments of the present invention.

Claims

1. A type of Bacillus coagulans ( Bacillus coagulans ), characterized in that, Its accession number is CGMCC No.36786.

2. The Bacillus coagulans according to claim 1, characterized in that, The nucleotide sequence of the Bacillus coagulans 16S rDNA is shown in SEQ ID NO:

1.

3. The Bacillus coagulans according to claim 1, characterized in that, The Bacillus coagulans possesses multiple antimicrobial lipopeptide genes and ACC deaminase genes.

4. The Bacillus coagulans according to claim 3, characterized in that, The multiple antimicrobial lipopeptide gene includes sfp , LPA-14 , ituD and fenB The ACC deaminase gene is acdS .

5. The Bacillus coagulans according to claim 1, characterized in that, The Bacillus coagulans possesses strong stress resistance, high fermentation activity, and the ability to produce antimicrobial lipopeptides and secrete plant growth hormones.

6. The Bacillus coagulans according to claim 1, characterized in that, The Bacillus coagulans has the effect of promoting crop yield and preventing diseases.

7. The Bacillus coagulans according to claim 6, characterized in that, The crops include one or more of the following: tomatoes, strawberries, cucumbers, chili peppers, peppers, celery, cauliflower, zucchini, Atractylodes lancea, citrus fruits, corn, peanuts, ginger, rice, potatoes, and sunflowers.

8. The Bacillus coagulans according to claim 6, characterized in that, The diseases mentioned include one or more of the following: bacterial wilt of tomato, wilt of tomato, damping-off of tomato, late blight of tomato, gray mold of tomato, anthracnose of chili pepper, anthracnose of pepper, root rot of celery, stem base rot of celery, downy mildew of cauliflower, bacterial soft rot of cauliflower, black spot of cauliflower, powdery mildew of zucchini, root rot of Atractylodes lancea, citrus canker, large leaf spot of corn, small spot disease of corn, and rust of corn.

9. A microbial inoculant, characterized in that, The microbial agent contains Bacillus coagulans as described in any one of claims 1-8.

10. The use of Bacillus coagulans as described in any one of claims 1-8 or the microbial agent as described in claim 9 in promoting crop yield and preventing diseases.

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