A hand-made tea-beating soap and a preparation method thereof

Abstract

The present invention relates to the technical field of handmade soap, specifically a pounded tea handmade soap, which is composed of the following raw materials in parts by weight: 25-30 parts of pounded tea stock solution, 80-90 parts of grease, 10-20 parts of sodium hydroxide, 20-40 parts of water, and 1-2 parts of natural additives. In the present invention, pounded tea stock solution is added during the preparation process of the handmade soap. It is rich in natural active ingredients such as tea polyphenols and tea saponins. The vegetable oils and minerals contained in sesame, peanuts, and ginger in the raw materials used for pounded tea can relieve the tight feeling that may occur after cleaning with the soap base, and have antibacterial and antioxidant effects, improving the use effect of the handmade soap.

Description

TECHNICAL FIELD

[0001] The present application relates to the technical field of handmade soap, in particular to a tea-beating handmade soap and a preparation method thereof. BACKGROUND

[0002] With the continuous improvement of people's health consciousness and skin care concept, consumers' requirements for the safety and naturalness of personal cleaning and skin care products are increasing. As a traditional and mild cleaning product, handmade soap has gradually become one of the mainstream products in the market due to its good cleaning power and skin affinity.

[0003] However, in order to pursue a longer shelf life, richer foam shape and brighter appearance, existing handmade soap products often add preservatives, synthetic foaming agents, artificial pigments, fragrances and other chemical ingredients during production. These chemical additives can optimize the use performance and sensory experience of the product to a certain extent, but long-term use can cause many adverse effects on the skin: for sensitive skin, it can easily cause skin redness, itching, stinging and other allergic reactions; for dry skin, chemical ingredients can damage the natural sebum film on the skin surface, exacerbate water loss, and cause dry, rough and tight skin; even for healthy skin, long-term contact with chemical additives can reduce the skin's self-repairing ability and cause chronic skin problems.

[0004] At the same time, the moisturizing and hydrating performance of existing handmade soap mostly depends on the addition of synthetic glycerin, lanolin and other chemical moisturizing agents. The moisturizing effect of such ingredients is short-lived and has poor compatibility with the skin, making it difficult to improve the dryness of the skin from the root.

[0005] Tea-beating, as a traditional and characteristic beverage in China, has a long history. It is mainly made of natural raw materials such as tea leaves, peanuts and sesame seeds. It is not only rich in nutrients such as tea polyphenols, vitamins and minerals, but also contains rich natural oil components such as peanut oil and sesame oil.

[0006] However, there is currently no process that combines traditional tea-beating with the manufacture of handmade soap, and this gap in the market needs to be developed. SUMMARY

[0007] The present application aims to develop a tea-beating handmade soap made of pure natural raw materials, which has the effects of mild cleaning, soothing and moisturizing, and antibacterial and itching relief.

[0008] To achieve the above-mentioned purposes, the technical solutions adopted by the present application are as follows:

[0009] The first aspect, the present application provides a kind of tea mortar handmade soap, it is characterized by: by following weight parts raw materials: tea mortar stock solution 25-30 parts, oil 80-90 parts, sodium hydroxide 10-20 parts, water 20-40 parts, natural additive 1-2 parts.

[0010] Further, the tea mortar handmade soap is characterized in that the natural additive includes a mass ratio of 2-4:1-3 of soapberry peel powder and licorice powder, and the oil includes a mass ratio of 50-55:25-30:5-8:7-10:3-5 of camellia oil, olive oil, coconut oil, palm oil and avocado oil.

[0011] Further, the tea mortar handmade soap is characterized in that the tea mortar stock solution preparation method comprises the following steps:

[0012] S1, tea leaves, mint leaves, wormwood leaves and houttuynia cordata are selected according to weight parts, 20-30 parts of tea leaves, 5-10 parts of mint leaves, 5-10 parts of wormwood leaves and 10-20 parts of houttuynia cordata dry powder, respectively, impurities, defective products and foreign matters are removed, and then they are washed with clean water and dried to remove surface moisture, and then mixed to obtain mixture 1;

[0013] S2, 20-25 parts of white sesame, 20-25 parts of black sesame and 20-25 parts of peanuts are selected and placed in a frying pan for frying and continuous stirring, frying for 5-8 min, then taken out and placed in a ventilated place for 1-2 h, mixed to obtain mixture 2, and ginger slices of 3-5 mm are obtained and reserved;

[0014] S3, ginger slices and salt are put into a mortar, and a pestle is used to grind them clockwise until ginger juice seeps out, mixture 1 is added, and grinding is continued for 3-5 min until they are crushed into powder, then mixture 2 is added and ground for 3-5 min, until they are ground into a fine paste, and grinding is continued for 15-20 min to make all raw materials fully broken and mixed to obtain a mixture;

[0015] S4, 80-90 ℃ water is added to the mixture, stirred uniformly, and then a 200-300 mesh filter screen is used to filter out residues and fibers to obtain a tea mortar stock solution.

[0016] Further, the tea mortar handmade soap is characterized in that the oil preparation method comprises the following steps: camellia oil, olive oil, coconut oil, palm oil and avocado oil are weighed and introduced into a constant temperature water bath, the temperature is set to 45-55 ℃, after melting, they are poured into a stirring container, the stirring speed is set to 250-350 r / min, and stirring is continued for 25-40 min to obtain oil, which is kept warm for use.

[0017] Further, the tea mortar handmade soap is characterized in that it is prepared by the following method:

[0018] S1: Weigh sodium hydroxide and water, add them into a stirring kettle, stir and mix them, then let them stand still. After the temperature drops to 35 - 40 °C, obtain mixture 3 for standby.

[0019] S2: Weigh oil and fat, add it into mixture 3, set the rotation speed to 400 - 350 r / min, and continuously stir for 1 - 2 h to obtain mixture 4.

[0020] S3: Weigh the raw pounded tea liquid, add it into mixture 4, continuously stir for 30 min, then add natural additives and continue to stir for 25 min to obtain soap liquor.

[0021] S4: Pour the soap liquor into a mold, let it stand still for 24 - 48 h. After cooling and forming, demold to obtain a handmade soap blank, put it into a thermostatic oven, set the temperature to 20 - 25 °C, and the relative humidity to 40% - 60%, and continuously carry out the aging treatment for 4 - 6 weeks, then cut it into pounded tea handmade soaps.

[0022] By combining the traditional pounded tea process with the preparation of handmade soap, the obtained pounded tea handmade soap has high safety, is mild and non - irritating, moisturizes and nourishes, and has multiple functions of antibacterial and antioxidant. Through detection, the highest free radical scavenging rate of the pounded tea handmade soap provided by the present invention can reach 72.5%, and the antibacterial rates against common bacteria such as Staphylococcus aureus, Escherichia coli, and Propionibacterium acnes are 93.5%, 90.2%, and 92.8% respectively. Specific embodiments

[0023] In order to make the purpose, technical solutions and advantages of the present application clearer, the following further details the present application in combination with embodiments. It should be understood that the specific embodiments described herein are only used to explain the present application and are not used to limit the present application. The reagents not specifically described in detail in the present application are all conventional reagents and can be obtained from commercial channels; the methods not specifically described in detail are all conventional experimental methods and can be known from the prior art.

[0024] It should be noted that the terms "first", "second", etc. in the specification, claims and drawings of the present invention are used to distinguish similar objects and do not necessarily describe a specific order or sequence, nor do they play a substantial limiting role on the subsequent technical features. It should be understood that such data can be interchanged under appropriate circumstances so that the embodiments of the present invention described here can be implemented in an order other than those illustrated or described here. In addition, the terms "comprising" and "having" and any variations thereof are intended to cover non - exclusive inclusion. For example, a process, method, system, product or device comprising a series of steps or units does not necessarily have to be limited to those steps or units clearly listed, but may include other steps or units not clearly listed or inherent to these processes, methods, products or devices.

[0025] For a better understanding of the present invention rather than limiting the scope thereof, all numbers representing amounts, percentages, and other numerical values used in this application should be understood to be modified by the word "about" in all cases. Therefore, unless otherwise specified, the numerical parameters listed in the specification and the appended claims are approximate values, which may vary depending on the desired properties to be obtained. Each numerical parameter should at least be regarded as obtained based on the reported significant figures and by the conventional rounding method.

[0026] The hand-made pounded tea soap of the present invention is composed of the following raw materials in parts by weight: 25 - 30 parts of pounded tea stock solution, 80 - 90 parts of grease, 10 - 20 parts of sodium hydroxide, 20 - 40 parts of water, and 1 - 2 parts of natural additive.

[0027] In some embodiments, the hand-made pounded tea soap includes sapindus peel powder and licorice powder with a mass ratio of 2 - 4:1 - 3, and the grease includes camellia oil, olive oil, coconut oil, palm oil, and avocado oil with a mass ratio of 50 - 55:25 - 30:5 - 8:7 - 10:3 - 5.

[0028] Among them, the sapindus peel powder has natural saponins with mild foaming power; tea polyphenols have antioxidant properties; licorice powder can anti-inflammatory and soothe; the extracts of mugwort and houttuynia cordata have antibacterial effects; coconut oil is rich in medium-chain fatty acids (such as lauric acid), which can form a thin hydrophobic film on the skin surface to reduce water loss in the stratum corneum. At the same time, its molecular structure is easy to penetrate into the stratum corneum to help soften dry skin.

[0029] In some embodiments, the preparation method of the pounded tea stock solution in the hand-made pounded tea soap includes the following steps:

[0030] S1. Select 20 - 30 parts of tea leaves, 5 - 10 parts of mint leaves, 5 - 10 parts of mugwort leaves, and 10 - 20 parts of dried houttuynia cordata powder by weight, and screen them separately to remove impurities, defective products, and foreign objects. After thoroughly washing with clean water and drying or baking to remove surface moisture, mix them to obtain mixture 1;

[0031] S2. Pretreatment: Select 20 - 25 parts of white sesame seeds, 20 - 25 parts of black sesame seeds, and 20 - 25 parts of peanuts and place them in a frying pan for frying and keep turning. After frying for 5 - 8 minutes, take them out and place them in a ventilated place for 1 - 2 hours, and mix them to obtain mixture 2. Cut ginger into ginger slices with a thickness of 3 - 5 mm and set aside;

[0032] S3. Grinding: Put the ginger slices and salt into a mortar, and grind them clockwise with a pestle until ginger juice seeps out. Add Mixture 1, and continue grinding for 3 - 5 min until it becomes powdery. Then add Mixture 2 and grind for 3 - 5 min until it becomes a delicate paste. Continue grinding for 15 - 20 min to fully break and mix all the raw materials to obtain a mixture;

[0033] S4. Filtering: Add water at 80 - 90 °C to the mixture, stir evenly, and then use a 200 - 300 - mesh filter to filter out the residues and fibers to obtain the original liquid of pounded tea.

[0034] In some embodiments, the method for preparing the oil in the pounded - tea handmade soap includes the following steps: Weigh camellia oil, olive oil, coconut oil, palm oil and avocado oil, pour them into a constant - temperature water bath, set the temperature at 45 - 55 °C, melt them and then pour them into a stirring container, set the rotation speed at 250 - 350 r / min, and continuously stir for 25 - 40 min to obtain the oil, and keep it warm for later use.

[0035] In some embodiments, the pounded - tea handmade soap is prepared by the following method:

[0036] S1: Weigh sodium hydroxide and water, add them to a stirring kettle, stir and mix them, and then let it stand. After the temperature drops to 35 - 40 °C, obtain Mixture 3 for later use;

[0037] S2: Weigh the oil and add it to Mixture 3, set the rotation speed at 400 - 350 r / min, and continuously stir for 1 - 2 h to obtain Mixture 4;

[0038] S3: Weigh the original liquid of pounded tea, add it to Mixture 4, continuously stir for 30 min, then add natural additives and continue stirring for 25 min to obtain the soap liquid;

[0039] S4: Pour the soap liquid into a mold, let it stand for 24 - 48 h. After cooling and forming, demold to obtain the handmade soap blank, put it into an incubator, set the temperature at 20 - 25 °C, relative humidity at 40% - 60%, and continuously carry out the aging treatment for 4 - 6 weeks, and then cut it into pounded - tea handmade soaps.

[0040] Test strains: Staphylococcus aureus, Escherichia coli, Propionibacterium acnes.

[0041] Example 1

[0042] For tea leaves, mint leaves, wormwood leaves, and houttuynia cordata, select 20 parts by weight of tea leaves, 5 parts by weight of mint leaves, 5 parts by weight of wormwood leaves, and 10 parts by weight of dry houttuynia cordata powder. Screen them respectively to remove impurities, defective products and foreign matters, wash them thoroughly with clean water and then dry or bake them to remove the surface moisture, and mix them to obtain Mixture 1;

[0043] Select 20 parts white sesame seeds, 20 parts black sesame seeds, and 20 parts peanuts, place them in a wok and stir-fry for 5 minutes. Then remove them and place them in a ventilated place for 1 hour. After mixing, you will get mixture 2. Cut ginger into 3-5 mm slices.

[0044] Place 3 slices of ginger and salt into a mortar and pestle, and grind clockwise with a pestle until the ginger juice seeps out. Add mixture 1 and continue grinding for 3 minutes until it is crushed into powder. Then add mixture 2 and grind for 3 minutes until it becomes a fine paste. Continue grinding for 15 minutes to fully crush and mix all the ingredients to obtain the mixture.

[0045] Add water at 80°C to the mixture, stir well, and then filter out the residue and fibers using a 200-300 mesh filter to obtain the Lei Cha original liquid.

[0046] Oil processing: Weigh 50 parts of camellia oil, 25 parts of olive oil, 5 parts of coconut oil, 7 parts of palm oil and 3 parts of avocado oil and put them into a constant temperature water bath. Set the temperature to 45 ℃. After melting, pour it into a stirring container, set the speed to 250 r / min and stir continuously for 25 min to obtain the oil. Keep it warm for later use.

[0047] Weigh out 10 parts of sodium hydroxide and 20 parts of water and add them to a stirring vessel. After stirring and mixing, let it stand until the temperature drops to 35 ℃ to obtain mixture 3, which is ready for use.

[0048] Weigh 80 parts of oil and add them to mixture 3. Set the speed to 400 r / min and stir continuously for 1 h to obtain mixture 4.

[0049] Weigh 25 parts of Lei Cha stock solution and add it to mixture 4. Stir continuously for 30 minutes, then add 2 parts of Sapindus mukorossi peel powder and 1 part of licorice powder and continue stirring for 25 minutes to obtain soap solution.

[0050] Pour the soap liquid into a mold (7*7.5*26.5cm), let it stand for 24 hours, and after cooling and solidification, demold to obtain the handmade soap blank. Place it in a constant temperature box, set the temperature to 20℃ and the relative humidity to 40%, and continue to cure for 4 weeks. Then cut it into Lei Cha handmade soaps.

[0051] Example 2

[0052] Raw material preparation: According to weight, select 30 parts tea leaves, 10 parts mint leaves, 10 parts mugwort leaves, and 20 parts dried houttuynia cordata powder. Screen each component separately to remove impurities, defective products, and foreign matter. Thoroughly wash with clean water and then air-dry or oven-dry to remove surface moisture. Mix to obtain mixture 1.

[0053] Pretreatment: Select 25 parts white sesame seeds, 25 parts black sesame seeds, and 25 parts peanuts and place them in a wok to stir-fry for 5 minutes. Then remove them and place them in a ventilated place for 1 hour. After mixing, you will get mixture 2. Cut ginger into 3-5 mm slices to get ginger slices.

[0054] Grinding: Put 5 slices of ginger and salt into a mortar and pestle and grind clockwise with a pestle until the ginger juice seeps out. Add mixture 1 and continue grinding for 3 minutes until it is crushed into powder. Then add mixture 2 into the mortar and grind for 3 minutes until it becomes a fine paste. Continue grinding for 15 minutes to fully crush and mix all the ingredients to obtain the mixture.

[0055] Filtration: Add water at 80-90℃ to the mixture, stir well, and then filter out the residue and fibers using a 200-300 mesh filter, keeping the original Lei Cha liquid for later use.

[0056] Oil processing: Weigh 50 parts of camellia oil, 25 parts of olive oil, 5 parts of coconut oil, 7 parts of palm oil and 3 parts of avocado oil and put them into a constant temperature water bath. Set the temperature to 45 ℃. After melting, pour it into a stirring container, set the speed to 250 r / min and stir continuously for 25 minutes to obtain the oil. Keep it warm for later use.

[0057] Weigh out 10 parts of sodium hydroxide and 20 parts of water and add them to a stirring vessel. After stirring and mixing, let stand until the temperature drops to 35-40℃ to obtain mixture 3.

[0058] Weigh 80 parts of the oil and add them to mixture 3. Set the speed to 400 r / min and stir continuously for 1 hour to obtain mixture 4.

[0059] Weigh 25 parts of Lei Cha stock solution and add it to mixture 4. Stir continuously for 30 minutes, then add 2 parts of Sapindus mukorossi peel powder and 1 part of licorice powder and continue stirring for 25 minutes to obtain soap solution.

[0060] Pour the soap liquid into a mold (7*7.5*26.5cm) and let it stand for 24 hours. After cooling and solidification, demold to obtain the handmade soap blank. Place it in a constant temperature box, set the temperature to 20℃ and the relative humidity to 40%, and continue to cure for 4 weeks. Then cut it into Lei Cha handmade soaps.

[0061] Example 3

[0062] The ingredients, including tea leaves, mint leaves, mugwort leaves, and houttuynia cordata, are selected in parts by weight. 15 parts of tea leaves, 10 parts of mint leaves, 2 parts of mugwort leaves, and 2 parts of dried houttuynia cordata powder are selected. These raw materials are screened separately to remove impurities, defective products, and foreign objects. They are thoroughly washed with clean water and then air-dried or oven-dried to remove surface moisture. After mixing, mixture 1 is obtained.

[0063] Select 10 parts of white sesame seeds, 10 parts of black sesame seeds, and 10 parts of peanuts, place them in a frying pan respectively, stir continuously, fry for 5 minutes, then take them out and place them in a ventilated place for 1 hour. After mixing, obtain mixture 2. Cut ginger into slices with a thickness of 3 - 5 mm to get ginger slices;

[0064] Pounding: Put 5 parts of ginger slices and salt into a pounding mortar, grind them clockwise with a pounding stick until ginger juice seeps out, add mixture 1 and continue to grind for 3 minutes until it becomes powdery. Then add mixture 2 into the pounding mortar and pound for 3 minutes until it becomes a delicate paste. Continue to pound for 15 minutes to fully break and mix all the raw materials to obtain a mixture;

[0065] Filtering: Add water at 80 °C to the mixture, stir evenly, and then use a 200 - 300 - mesh filter to filter out residues and fibers, leaving the original pounded tea liquid for standby.

[0066] Oil treatment: Weigh 50 parts of camellia oil, 25 parts of olive oil, 5 parts of coconut oil, 7 parts of palm oil, and 3 parts of avocado oil, pour them into a constant - temperature water bath, set the temperature to 45 °C, melt them and then pour them into a stirring container. Set the rotation speed to 250 r / min and stir continuously for 25 minutes to obtain oil, and keep it warm for use.

[0067] Weigh 10 parts of sodium hydroxide and 20 parts of water, add them to a stirring kettle, stir and mix, then let it stand. Wait until the temperature drops to 35 °C to obtain mixture 3;

[0068] Weigh 80 parts of oil and add it to mixture 3, set the rotation speed to 400 r / min, stir continuously for 1 hour, and then mix to obtain mixture 4;

[0069] Weigh 25 parts of the original pounded tea liquid, add it to mixture 4, stir continuously for 30 minutes, then add 2 parts of sapindus peel powder and 1 part of licorice powder, and continue to stir for 25 minutes to obtain soap liquid;

[0070] Pour the soap liquid into a mold (specification: 7 * 7.5 * 26.5 cm), let it stand for 24 hours. After cooling and forming, demold to obtain a handmade soap blank, put it into a constant - temperature oven, set the temperature to 20 °C and the relative humidity to 40%, and continue the aging treatment for 4 weeks, then cut it into pounded - tea handmade soaps.

[0071] Comparative example 1: The difference between this comparative example and Example 1 is that: this comparative example does not contain the original pounded tea liquid.

[0072] Comparative example 2: The difference between this comparative example and Example 1 is that: this comparative example replaces the original pounded tea liquid with a matcha aqueous solution with a mass ratio of 8:1.

[0073] Comparative example 3: The difference between this comparative example and Example 1 is that: this comparative example replaces coconut oil with glycerol.

[0074] Quality evaluation of the examples: In accordance with the national standard QB / T2485-2008 for soap, the sensory and physicochemical indicators of the Lei Cha handmade soaps in Examples 1 to 3 were determined. The test results are shown in Table 1. The physicochemical performance indicators of Examples 1 to 3 are all qualified.

[0075] Table 1

[0076]

[0077] Example 4: Testing the moisturizing and antioxidant capabilities of Lei Cha handmade soap

[0078] 4.1 Detection of changes in skin moisture content

[0079] Ten healthy volunteers (5 males and 5 females, aged 20-35 years, all with dry skin) were selected. Under constant temperature and humidity (temperature 25℃, humidity 50%), the inner forearm was divided into two 2cm×2cm test areas. The six samples of Examples 1-3 and Comparative Examples 1-3 were labeled as areas 1-6 respectively. Before the test, the test areas were cleaned with purified water and allowed to air dry for 30 minutes. The initial moisture content (T0) was measured using a skin moisture meter (Guining ASA-M100). Then, the corresponding group of handmade soaps were used to clean the test areas in the same way (rub for 30 seconds, rinse with warm water, and air dry). The skin moisture content of each area was measured at 1h (T1), 3h (T2), and 6h (T3) after cleaning. Each test was repeated 3 times, and the average value was taken. The rate of change of moisture content was calculated (rate of change = (Tt-T0) / T0×100%, t=1, 3, 6).

[0080] 4.2 DPPH (1,1-diphenyl-2-trinitrophenylhydrazine) free radical scavenging rate detection

[0081] DPPH free radicals appear deep purple in organic solvents (commonly ethanol and methanol), and their unpaired electrons in their molecular structure give them a strong characteristic absorption peak at 517 nm. When an antioxidant is added to the system, the antioxidant donates hydrogen atoms, which combine with the unpaired electrons of the DPPH free radical, reducing DPPH to a colorless / pale yellow stable compound without free radicals. The free radical scavenging rate can be calculated by measuring the difference between the absorbance of the system after adding handmade soap and the absorbance of the DPPH solution without handmade soap. This embodiment utilizes this characteristic to detect the antioxidant capacity of handmade soap.

[0082] Take 5g of each of the handmade soaps from Examples 1-3 and Comparative Examples 1-3, add 100mL of deionized water, and extract ultrasonically for 30min (power 200W, temperature 30℃). After filtration, obtain the soap extract. Prepare a 0.1mmol / L DPPH ethanol solution. Mix 2mL of DPPH solution with 2mL of soap extracts from different groups and label it as Sample A (1-6). Let it stand in the dark for 30min, and measure the absorbance at 517nm using a UV spectrophotometer. Simultaneously, set up sample blank B (absorbance of 2mL soap extract + 2mL ethanol solution) and label it as Sample Blank B (1-6). Set up blank group C (2mL DPPH solution + 2mL ethanol) and label it as Blank C (1-6). Measure the absorbance according to the method for Sample A. Each experiment was repeated 3 times, and the average value was taken. The formula for calculating the DPPH free radical scavenging rate is as follows:

[0083] DPPH free radical scavenging rate = [1 - (AB) / C] × 100%;

[0084] The test results are shown in Table 1. Compared with Comparative Examples 1-3, Examples 1-3 showed significant advantages in terms of the immediacy and duration of skin moisture retention after cleansing and antioxidant activity. Furthermore, Example 2 had the best overall performance among all tested samples.

[0085] Table 2

[0086]

[0087] Example 3: Detection of the antibacterial effect of Lei Cha handmade soap

[0088] 3.1 Raw material preparation:

[0089] Take 10g of each of the handmade soaps prepared in Examples 1-3 and Comparative Examples 1-3 and grind them into powder for later use.

[0090] Test strain

[0091] Staphylococcus aureus, Escherichia coli, and Propionibacterium acnes were selected, all of which are common pathogenic bacteria on the skin, and were provided by the Microbial Culture Collection Center.

[0092] Prepare the nutrient broth culture medium and nutrient agar culture medium with the following formulas:

[0093] Nutrient broth culture medium: 10g peptone, 10g peptone, 5g sodium chloride (NaCl), 1000mL distilled water

[0094] Nutrient agar medium: 10g peptone, 3g beef extract, 5g sodium chloride (NaCl), 15-20g agar powder, 1000mL distilled water

[0095] 3.2 Antibacterial activity test

[0096] Take 2g of handmade soap powder from Examples 1-3 and Comparative Examples 1-3 respectively, add 20mL of sterile physiological saline, and sonicate for 30min (power 200W, temperature 30℃). Then filter with a 0.22 μm sterile filter membrane to obtain sterile sample solutions for each experimental group.

[0097] Staphylococcus aureus, Escherichia coli, and Propionibacterium acnes were inoculated into nutrient broth medium and cultured at 37°C with shaking for 18-24 hours to obtain bacterial suspensions. The bacterial suspensions were then diluted with sterile physiological saline to a concentration of 1×10⁻⁶. 6 CFU / mL standard bacterial suspension;

[0098] Mix 2 mL of each sterile sample solution with 2 mL of standard bacterial suspension and incubate at 37°C for 2 hours. A blank control group (2 mL sterile saline + 2 mL standard bacterial suspension) is also set up. After the incubation period, take 1 mL of the mixture and serially dilute it with sterile saline (10⁻¹, 10⁻², 10⁻³). Spread 0.1 mL of the 10⁻³ solution evenly onto a nutrient agar plate, creating three parallel plates. Incubate the plates at 37°C for 24 hours for Staphylococcus aureus and Escherichia coli, and 48 hours for Propionibacterium acnes. Count the number of colonies (CFU) on the plates.

[0099] Antibacterial rate calculation: Antibacterial rate = (1 - number of colonies in the sample group / number of colonies in the blank control group) × 100%. Each experiment was repeated 3 times, and the average value was taken.

[0100] Table 3

[0101]

[0102] As shown in Table 2, the Lei Cha handmade soaps of Examples 1-3 have excellent antibacterial properties: the antibacterial rate against Staphylococcus aureus, Escherichia coli, and Propionibacterium acnes all exceeded 85%, with Example 2 showing the best performance, with antibacterial rates against the three pathogenic bacteria reaching 93.5±1.8%, 90.2±2.0%, and 92.8±1.9%, respectively. This indicates that the Lei Cha handmade soap of the present invention can effectively inhibit common skin pathogens and reduce acne and inflammation caused by bacterial growth.

[0103] In summary, the Lei Cha handmade soaps of Examples 1-3 of this invention not only possess excellent moisturizing and antioxidant properties, but also exhibit significant antibacterial effects, effectively inhibiting common skin pathogens and further enhancing the product's skincare value. This better meets consumers' demand for natural, safe, and multifunctional cleansing and skincare products. Among these, the formulation in Example 2 is the optimal solution, exhibiting the most prominent antibacterial effect.

Claims

1. A handcrafted pounded tea soap, characterized in that: It consists of the following raw materials in parts by weight: 25 - 30 parts of pounded tea stock solution, 80 - 90 parts of grease, 10 - 20 parts of sodium hydroxide, 20 - 40 parts of water, and 1 - 2 parts of natural additive.

2. The Lei Cha handmade soap according to claim 1, characterized in that, The natural additive includes sapindus peel powder and licorice powder with a mass ratio of 2 - 4:1 - 3. The grease includes camellia oil, olive oil, coconut oil, palm oil, and avocado oil with a mass ratio of 50 - 55:25 - 30:5 - 8:7 - 10:3 - 5.

3. The Lei Cha handmade soap according to claim 1, characterized in that, The preparation method of the pounded tea stock solution includes the following steps: S1. By weight, select 20 - 30 parts of tea leaves, 5 - 10 parts of mint leaves, 5 - 10 parts of wormwood leaves, and 10 - 20 parts of houttuynia cordata dry powder. Screen them respectively to remove impurities, defective products, and foreign objects. After thoroughly washing with clear water, dry or bake them to remove surface moisture, and then mix to obtain mixture 1. S2. Select 20 - 25 parts of white sesame seeds, 20 - 25 parts of black sesame seeds, and 20 - 25 parts of peanuts, place them in a frying pan and stir continuously. Fry for 5 - 8 min, then take them out and place them in a ventilated place for 1 - 2 h, and mix to obtain mixture 2. Cut ginger into ginger slices with a thickness of 3 - 5 mm and set aside. S3. Put the ginger slices and salt into a mortar, grind them clockwise with a pestle until ginger juice seeps out, add mixture 1, and continue to grind for 3 - 5 min until it becomes powdery. Then add mixture 2 and grind for 3 - 5 min until it becomes a delicate paste, and continue to grind for 15 - 20 min to fully break and mix all the raw materials to obtain a mixture. S4. Add water at 80 - 90 °C to the mixture, stir evenly, and then filter out residues and fibers with a 200 - 300 - mesh sieve to obtain the pounded tea stock solution.

4. The Lei Cha handmade soap according to claim 1, characterized in that, The preparation method of the grease includes the following steps: Weigh camellia oil, olive oil, coconut oil, palm oil, and avocado oil, pour them into a constant temperature water bath, set the temperature at 45 - 55 °C, melt them and then pour them into a stirring container. Set the rotation speed at 250 - 350 r / min and continuously stir for 25 - 40 min to obtain the grease, and keep it warm for use.

5. The Lei Cha handmade soap according to any one of claims 1-4, characterized in that, It is prepared by the following method: S1: Weigh sodium hydroxide and water and add them to a stirring kettle. Stir and mix, then let it stand. After the temperature drops to 35 - 40 °C, obtain mixture 3 and set it aside. S2: Weigh the grease and add it to mixture 3. Set the rotation speed at 400 - 350 r / min and continuously stir for 1 - 2 h to obtain mixture 4. S3: Weigh the pounded tea stock solution, add it to mixture 4, continuously stir for 30 min, then add the natural additive and continue to stir for 25 min to obtain the soap solution. S4: Pour the soap solution into a mold, let it stand for 24 - 48 h. After cooling and forming, demold to obtain the handmade soap blank, put it into a constant temperature oven, set the temperature at 20 - 25 °C, and the relative humidity at 40% - 60%, and continuously carry out the ripening treatment for 4 - 6 weeks, and then cut it into pounded tea handmade soaps.

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