A stable genetic transformation method using rubber grass callus as explant

By using the genetic transformation method of rubber grass callus tissue, the problem of low transformation efficiency of rubber grass has been solved, and homozygous transgenic plants have been obtained efficiently with high positive detection rate, fast growth rate and high propagation efficiency, which is suitable for the optimization of rubber grass genetic transformation system.

CN122128359APending Publication Date: 2026-06-02GUIZHOU UNIV
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Applications(China)
Current Assignee / Owner
GUIZHOU UNIV
Filing Date
2026-05-06
Publication Date
2026-06-02

AI Technical Summary

Technical Problem

Existing methods for genetic transformation of rubber grass are inefficient, making it difficult to obtain homozygous transgenic materials. Furthermore, self-incompatibility in rubber grass leads to severe strain mixing, resulting in low transformation efficiency and making it difficult to meet the demand for large quantities of transgenic rubber grass materials.

Method used

Rubber grass callus was used as explants. Through antibiotic screening and optimization of culture medium composition, including steps such as callus induction, proliferation, infection, co-culture, screening and rooting culture, the transformation efficiency and homozygosity were improved. Homozygous positive cell lines were obtained by Agrobacterium infection and antibiotic screening.

Benefits of technology

It has achieved efficient acquisition of a large number of transgenic plants with high conversion rate, low malformation rate, positive detection rate of over 95%, short genetic transformation cycle, and strong embryogenicity and proliferation ability of rubber grass callus tissue, which can rapidly propagate a large number of positive seedlings.

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Abstract

This invention discloses a stable genetic transformation method using rubber grass callus as explants. The steps include: sterilized Xinjiang rubber grass seeds undergoing callus induction, callus proliferation culture, GV3101 Agrobacterium transformation, preliminary screening culture, selection of shoot-forming culture, rooting culture, positive seedling rejuvenation culture, and identification. Using the genetic transformation method described in this invention, rubber grass callus cell lines with excellent embryogenic characteristics can be obtained. Using these embryogenic cell lines as genetic transformation material, stable genetically transformed cell lines can be obtained in a short time. After shoot-forming and rooting culture, a considerable number of genetically transformed plants can be obtained in a short period.
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