High-yield beta-nicotinamide mononucleotide genetically engineered strain, construction method and application thereof
By integrating and overexpressing the nicotinic acid phosphoribosyltransferase gene PncB and the nicotinic acid nucleotide adenylate transferase gene NadD in Bacillus subtilis, a stable high-yield NMN engineered strain was constructed, solving the problem of easy plasmid loss and achieving a significant increase in NMN yield and a reduction in production costs.
CN122146554APending Publication Date: 2026-06-05TIANJIN UNIV OF SCI & TECH
Patent Information
- Authority / Receiving Office
- CN · China
- Patent Type
- Applications(China)
- Current Assignee / Owner
- TIANJIN UNIV OF SCI & TECH
- Filing Date
- 2026-03-12
- Publication Date
- 2026-06-05
AI Technical Summary
Technical Problem
In existing technologies, genetically engineered strains have the problem of plasmid loss during NMN production, which leads to unstable genetic traits and affects yield and production performance.
Method used
A stable, high-yield NMN engineered strain was constructed by overexpressing the nicotinic acid phosphoribosyltransferase gene PncB and the nicotinic acid nucleotide adenylate transferase gene NadD in Bacillus subtilis WB600 using a genome integration method. Gene expression was performed using the genome integration plasmid pDG364-0 to avoid plasmid loss.
Benefits of technology
It improved the yield and genetic stability of NMN, shortened the fermentation cycle, reduced production costs, and significantly increased the yield and production efficiency of NMN.
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Abstract
The application discloses a kind of by genome integration overexpression nicotinic acid phosphoribosyltransferase gene PncB, Nicotinic acid nucleotide adenylyltransferase gene NadD, Quinoline synthase gene NadA, Quinolinic acid phosphoribosyltransferase gene NadC, NAD+ synthetase NadE, NAD+ synthetase gene in Francisella tularensis FtNadE Method for obtaining stable high-yield β-nicotinamide mononucleotide (NMN) engineering strain by one or two genes in and applying it to fermentation production of NMN, and optimal high-yield strain B. subtilis PncB-NadD 5L fermenter batch feeding fermentation NMN yield can reach 4.547 g / L, compared with chassis strain Bacillus subtilis WB600 yield 1.034 g / L is increased by 339.75%. The problem of unstable performance of engineering strain is solved, and the production performance of strain NMN is improved, which has high industrial application value.
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