Method for quantifying partial reprogramming pig somatic cells and application thereof
By quantitatively reprogramming pig somatic cells, their epigenetic age can be reversed to 57%-77%. Combined with a pig-specific epigenetic clock model and gene editing, the problem of high abortion rate in pig somatic cells during multiple gene editing processes has been solved, achieving efficient cloning production.
Patent Information
- Application Number
- CN202610436530.3
- Authority / Receiving Office
- CN · China
- Patent Type
- Applications(China)
- Current Assignee / Owner
- Filing Date
- 2026-04-02
- Publication Date
- 2026-06-05
AI Technical Summary
Existing technologies cannot effectively address the problems of insufficient editing tolerance and high abortion rates in pig somatic cells due to aging during multiple gene editing. They also lack objective and quantitative assessment standards for cell aging, resulting in unstable reprogramming schemes and hindering high-efficiency industrialization.
This invention provides a method for quantitative partial reprogramming of porcine somatic cells, which reverses the epigenetic age of porcine somatic cells to 57%-77% using mRNA or small molecule compositions, constructs a porcine-specific epigenetic clock model, screens high-quality nuclear donor cells, performs gene editing and somatic cell nuclear transfer, and reduces the abortion rate.
It reduced the early abortion rate of porcine somatic cell SCNT from 40% to <20%, established a complete solution from assessment and optimization to production, broke through the core bottleneck of low porcine cloning efficiency, and provided efficient industry practice guidance.