A comprehensive prevention and control method for ginger wilt disease and a matching planting system

Abstract

The application discloses a ginger-wilt disease comprehensive prevention and control method and a matching planting system of ginger-Asteraceae crop interplanting and biocontrol bacteria cooperation. The ginger-wilt disease comprehensive prevention and control method adopts a four-time-point process of "pre-sowing Asteraceae plants, ginger transplanting at the same period, root dipping (ginger seed soaking) / hole applying biocontrol bacteria, timing cutting for in-situ biological fumigation before flood season, and drip irrigation for bacteria supplementing", constructs a rhizosphere symbiosis-disease inhibition zone, utilizes the root secretion and volatile substances (such as thiothiophene, sesquiterpene lactone, etc.) of Asteraceae plants and the straw cutting and returning biological fumigation effect, cooperates with Bacillus-Trichoderma-Pseudomonas / Streptomyces compound bacterial agent, and significantly inhibits the colonization and migration of Ralstonia solanacearum. Compared with conventional monoculture, the application can reduce the disease index by 40-70%, increase the yield of commercial ginger by 10-22%, and reduce the use intensity of chemical pesticides. The application is compatible with existing drip irrigation, mulching and high-ridge cultivation, and is suitable for large-area application in the Sichuan-Chongqing high-humidity continuous cropping ecological niche.

Description

Technical Field

[0001] This invention relates to the field of crop cultivation technology, specifically to a comprehensive control method and supporting planting system for ginger wilt disease by intercropping ginger with Asteraceae crops and synergistic biocontrol bacteria. Background Technology

[0002] In the Sichuan-Chongqing region, continuous cropping of ginger and the coexistence of high humidity environments make it easy for the spatial and temporal fluctuations of soil aeration and moisture, as well as surface runoff, to promote the spread and rhizosphere colonization of Ralstonia solanacearum, thereby affecting ginger yield and increasing the disease index of ginger.

[0003] To increase ginger yield and reduce disease index, traditional chemical control has poor sustainability during the rainy season and may induce resistance; single biological control or single crop cultivation is difficult to stably improve the root zone ecological niche.

[0004] Asteraceae crops generally have root exudates and volatile secondary metabolites (such as thiothiophene, sesquiterpene lactones, monoterpenes, etc.), and returning them to the field after harvesting has the potential for biofumigation; at the same time, their shading and cooling effects and interception effects can slow down the connectivity of surface water films during the rainy season.

[0005] Therefore, by combining strip intercropping of Asteraceae plants with compound biocontrol bacteria in a timely manner, it is expected to establish a comprehensive barrier of "plant diversity - disease-suppressing microgroups - hydrothermal buffer" to increase ginger yield and reduce ginger disease index. Summary of the Invention

[0006] The purpose of this invention is to provide a comprehensive control method and supporting planting system for ginger wilt disease through intercropping ginger with Asteraceae crops and synergistic biocontrol bacteria. Without significantly increasing production costs, a reproducible and scalable ginger disease-suppressing ecosystem is constructed in a high-humidity continuous cropping niche, achieving stable yields and increased income while reducing chemical inputs.

[0007] The objective of this invention is achieved as follows:

[0008] A method for integrated control of ginger wilt disease using intercropping of ginger and Asteraceae crops and synergistic biocontrol agents includes the following steps:

[0009] S1. Pre-planting of Asteraceae crops: 15–30 days before ginger transplanting, make beds with a ratio of ginger rows to Asteraceae crop rows of (2–4):1, and plant Asteraceae crops in each strip with 1–2 rows of Asteraceae crops.

[0010] S2. Root dipping, seed soaking, or hole application of biocontrol agents: Biocontrol agents include liquid and solid inoculants. Apply at a concentration of 10% when transplanting ginger (before sowing). 7 -10 8For root dipping or seed soaking in CFU·mL⁻¹ bacterial solution, apply the solution for 20-30 minutes. When applying in holes, mix the solid bacterial agent with the soil in the hole, with a mass ratio of (0.5–2.0):100.

[0011] S3. Pre-flood harvesting and in-situ biological fumigation: 35–55 days after transplanting, before the main flood season, harvest the Asteraceae crops at a height of 10–20 cm from the ground, with a fresh weight of 3–6 t·ha. -1 Crush and cover the surface in place or shallowly till to a depth of 5–8 cm;

[0012] S4. Drip irrigation with probiotics: 45–75 days after ginger transplanting, perform drip irrigation with probiotics 1–3 times at 7–14 day intervals, with each application containing ≥ 10¹¹ CFU·ha. -1 ;

[0013] S5. Water, fertilizer and field condition control: Maintain rhizosphere pH between 6.0 and 7.0, and control soil moisture content at 60%-75% of field capacity. The furrows should have longitudinal drainage.

[0014] The Asteraceae crops mentioned are at least one of marigold, garland chrysanthemum, artemisia, calendula, and sunflower.

[0015] The plant density for the Asteraceae strips is: 8–12 plants per m² for marigolds. -2 The plant density of garland chrysanthemum is 12–16 plants per m. -2 Artemisia annua is 6–10 plants per m -2 The width of the ginger rows is 60–80 cm, and the spacing between the rows of Asteraceae plants is 25–35 cm.

[0016] The biocontrol bacteria include one or more of Bacillus, Trichoderma, Pseudomonas, and Streptomyces, and the viable count ratio of Bacillus:Trichoderma:Pseudomonas / Streptomyces is (2–5):(1–3):(0.5–2); the solid bacterial agent is an adsorption-type solid preparation of double-layer microcapsules or porous carriers (zeolite / biochar), with a water content ≤ 10% and a viable count ≥ 1×10⁻⁶. 8 CFU·g -1 .

[0017] The Bacillus species is any one of Bacillus velezensis, B. amyloliquefaciens, and B. subtilis; the Trichoderma species is any one of Trichoderma harzianum and T. asperellum; and the Pseudomonas species is any one of Pseudomonas chlororaphis and P. fluorescens. The bacterial suspension includes B. velezensis, T. harzianum, and P. chlororaphis, with a viable cell ratio of 3:1:1. The solid bacterial agent is a composite bacterial strain obtained by combining B. velezensis, T. harzianum, and P. chlororaphis in a viable cell ratio of 3:1:1. This composite bacterial strain is then loaded onto microporous zeolite / biochar to obtain particles with a diameter of 0.5–2.5 mm and a B. velezensis concentration ≥2 × 10⁻⁶. 9 CFU·g⁻¹, concentration of T. harzianum ≥ 5 × 10⁻¹ 8 CFU·g⁻¹, P. chlororaphis concentration ≥1×10⁻¹ 9 CFU·g⁻¹, the mass ratio of microporous zeolite to biochar is 1:1.

[0018] Asteraceae crops should undergo a second height-limited harvesting 65–85 days after transplanting, yielding crops with a fresh weight of 2–4 t·ha. -1 Cover the furrows with Asteraceae crops to maintain ventilation, light, and the continuity of the "disease-suppressing zone"; complete a supplementary drip irrigation within 24–72 hours after mowing and biological fumigation to enhance the temporal synergy of decomposition and release of antibacterial bacteria.

[0019] When pre-sowing Asteraceae crops in S1, a boundary buffer zone should be set up, that is, marigolds should be planted around the perimeter of the plot with a width of 0.5–1.5m and a density of 10–14 plants per m. -2 This forms a barrier to prevent the spread of diseases.

[0020] A supporting planting system for the integrated control of ginger wilt disease using ginger-Asteraceae intercropping and biocontrol bacteria includes raised-ridge mulching cultivation, pre-sowing of Asteraceae crops, pre-harvesting and on-site biological fumigation, and drip irrigation for bacterial supplementation. The drip irrigation tape is located 10–15 cm to the side of the ginger row root zone, and the Asteraceae crop rows are staggered at the outer edge of the mulch or within the mulch holes. The system is accompanied by an annual operation schedule of two height-limited harvests, one shallow tillage, and 2–3 drip irrigations for bacterial supplementation.

[0021] The overlap rate of the canopy between the ginger rows and the rows of Asteraceae crops is 15–35%.

[0022] The beneficial effects of this invention are:

[0023] 1. This invention adopts a four-time point process of "pre-sowing Asteraceae crops → simultaneous root dipping (soaking ginger seeds) / application of biocontrol bacteria in holes during ginger transplanting → timely harvesting and on-site biological fumigation before the flood season → drip irrigation supplementation of bacteria", constructing a rhizosphere symbiotic-disease-suppressing zone at a strip ratio of 2:1–4:1 (ginger row: Asteraceae crop row). It utilizes the root secretions and volatiles of Asteraceae crops (such as thiothiophene, sesquiterpene lactones, etc.) and the biological fumigation effect of straw harvesting, in conjunction with the compound inoculant of Bacillus-Trichoderma-Pseudomonas / Streptomyces, to significantly inhibit the colonization and migration of Ralstonia solanacearum;

[0024] 2. This invention reduces the disease index of ginger by 40-70%, increases the marketability, and increases yield by 10-22%;

[0025] 3. This invention reduces surface runoff and rhizosphere supersaturation duration during the rainy season, shortens the duration of the interconnected water film, enhances rhizosphere antagonistic bacterial colonization, and significantly reduces pathogen copy number;

[0026] 4. This invention is compatible with existing drip irrigation / mulching / high ridge systems, reducing reliance on chemical agents. Detailed Implementation

[0027] The present invention will be further described below with reference to the embodiments.

[0028] Example 1: Strip Configuration and Time Flow

[0029] Location: Leshan City, Sichuan Province; Soil: Purple soil; Previous crop: Ginger;

[0030] Layout: Ginger: Asteraceae crops = 3:1 strips. Ginger ridges are 70 cm wide with a plant spacing of 20–25 cm; Asteraceae crops (marigold + garland chrysanthemum) are planted in two staggered rows with a row spacing of 30 cm.

[0031] A method for integrated control of ginger wilt disease using intercropping of ginger and Asteraceae crops and synergistic biocontrol agents includes the following steps:

[0032] S1. Pre-sowing of Asteraceae crops: 20 days before ginger transplanting, prepare beds in a 3:1 ratio of ginger rows to Asteraceae crop rows, and plant marigolds / garland chrysanthemum, with 2 rows of marigolds / garland chrysanthemum per bed; when pre-sowing Asteraceae crops, establish a boundary buffer zone, i.e., plant marigolds 1 m wide around the perimeter of the plot at a density of 12 plants per m². -2 This forms a barrier to prevent the spread of diseases.

[0033] S2. Root dipping or seed soaking, or application of biocontrol agents in planting holes: When transplanting ginger (before sowing), use a 10% concentration of biocontrol agents. 8 CFU·mL -1 Dip the roots in the bacterial solution for 1-2 minutes (soak ginger seeds for 20-30 minutes).

[0034] S3. Pre-flood harvesting and in-situ biological fumigation: 40 days after transplanting, before the main flood season, harvest the Asteraceae crops at a height of 15 cm above the ground, using a fresh weight of 5 t·ha of Asteraceae crops. -1 Crush the surface material and cover it with the mortar or shallowly till it to a depth of 5 cm.

[0035] S4. Drip irrigation with probiotics: Apply probiotics via drip irrigation once each at 48 days and 62 days after ginger transplanting, with each application containing ≥10¹¹ CFU·ha. -1 ;

[0036] S5. Water, fertilizer and field condition control: Maintain rhizosphere pH between 6.0 and 7.0, and control soil moisture content at 60%-75% of field capacity. The furrows should have longitudinal drainage.

[0037] The plant density for the Asteraceae strips is: 8–12 plants per m² for marigolds. -2 The plant density of garland chrysanthemum is 12–16 plants per m. -2 Artemisia annua is 6–10 plants per m -2 The ginger rows are 70 cm wide, with a plant spacing of 20–25 cm; the Asteraceae crops (marigold + garland chrysanthemum) are planted in two alternating rows with a row spacing of 30 cm.

[0038] The root dipping, or seed soaking, uses a bacterial solution comprising B. velezensis, T. harzianum, and P. chlororaphis, with a viable count ratio of velezensis, T. harzianum, and P. chlororaphis of 3:1:1.

[0039] The biocontrol agent applied in the pit uses solid bacteria. The solid bacterial agent is a compound of *B. velezensis*, *T. harzianum*, and *P. chlororaphis* in a viable count ratio of 3:1:1. This compound bacteria is then loaded onto microporous zeolite / biochar to produce particles with a diameter of 0.5–2.5 mm, a moisture content ≤ 10%, and a viable count ≥ 1 × 10⁻⁶. 8 CFU·g -1 ;

[0040] The concentration of B. velezensis is ≥2×10 9 CFU·g -1 The concentration of T. harzianum is ≥5×10 8 CFU·g -1 The concentration of P. chlororaphis is ≥1×10 9 CFU·g -1 The mass ratio of microporous zeolite to biochar is 1:1.

[0041] Frequency: One root dip and two drip irrigations are optimal; further increasing the frequency yields diminishing returns.

[0042] Asteraceae crops should undergo a second height-limited harvesting 75 days after transplanting, yielding crops with a fresh weight of 3 tons per hectare. -1 Cover the furrows with Asteraceae crops to maintain ventilation, light, and the continuity of the "disease-suppressing zone"; complete a supplementary drip irrigation within 48 hours after mowing and biological fumigation to enhance the temporal synergy of decomposition and release of antibacterial bacteria.

[0043] Example 2: The only difference from Example 1 is the combination of Asteraceae plants, as detailed below:

[0044] The following combinations of plants from the Asteraceae family are used: A. Marigold; B. Chrysanthemum greens; C. Artemisia argyi; D. Marigold + Artemisia argyi.

[0045] Conclusion: The effects of the above-mentioned components were not as good as those of Example 1 (marigold + garland chrysanthemum). Example 1 achieved the best balance between disease suppression and economic benefits (garland chrysanthemum can be harvested), with a 61% reduction in the disease index. Treatment C was effective in suppressing disease, but plant height needed to be controlled to avoid excessive shading.

[0046] Comparative Example 1: The only difference from Example 1 is that it lacks Asteraceae and only contains biocontrol bacteria, as detailed below:

[0047] Root dip or seed soaking, or application of biocontrol agents in planting holes: When transplanting ginger (before sowing), 10 8 Dip roots in CFU·mL⁻¹ bacterial solution for 1-2 minutes (soak ginger seeds for 20-30 minutes).

[0048] Drip irrigation with probiotics: Apply probiotics via drip irrigation once at 48 days and 62 days after ginger transplanting, with each application containing ≥ 10¹¹ CFU·ha. -1 ;

[0049] Water, fertilizer and field condition control: Maintain rhizosphere pH between 6.0 and 7.0, and control soil moisture content at 60%-75% of field capacity, with longitudinal drainage in furrows.

[0050] The root dip, or seed soaking, uses a bacterial solution comprising B. velezensis, T. harzianum, and P. chlororaphis, with a viable count ratio of velezensis, T. harzianum, and P. chlororaphis of 3:1:1.

[0051] The biocontrol agent applied in the pit uses solid bacteria. The solid bacterial agent is a compound of *B. velezensis*, *T. harzianum*, and *P. chlororaphis* in a viable count ratio of 3:1:1. This compound bacteria is then loaded onto microporous zeolite / biochar to produce particles with a diameter of 0.5–2.5 mm, a moisture content ≤ 10%, and a viable count ≥ 1 × 10⁻⁶. 8 CFU·g -1 ;

[0052] The concentration of B. velezensis is ≥2×10 9 CFU·g -1 The concentration of T. harzianum is ≥5×10 8 CFU·g -1 The concentration of P. chlororaphis is ≥1×10 9 CFU·g -1 The mass ratio of microporous zeolite to biochar is 1:1.

[0053] Frequency: One root dip and two drip irrigations are optimal; further increasing the frequency yields diminishing returns.

[0054] The post-flood efficacy and disease resurgence were not as good as in Example 1, with the disease index decreasing by only 20–28%.

[0055] Comparative Example 2: The only difference from Example 1 is that it contains Asteraceae family bacteria but lacks biocontrol bacteria, as detailed below:

[0056] A method for integrated control of ginger wilt disease using intercropping of ginger and Asteraceae crops and synergistic biocontrol agents includes the following steps:

[0057] Pre-sowing of Asteraceae crops: 20 days before ginger transplanting, create raised beds with a ginger row to Asteraceae crop row ratio of 3:1, and plant marigolds / garland chrysanthemum, with one row of marigolds / garland chrysanthemum per strip; when pre-sowing Asteraceae crops, establish boundary buffer zones, i.e., plant marigolds 1m wide around the perimeter of the plot at a density of 12 plants per m². -2 This forms a barrier to prevent the spread of diseases.

[0058] Pre-flood harvesting and in-situ biological fumigation: 40 days after transplanting, before the main flood season, harvest the Asteraceae crops at a height of 15 cm above the ground, using 5 t·ha of fresh Asteraceae crops. -1 Crush the surface material and cover it with the mortar or shallowly till it to a depth of 5 cm.

[0059] Water, fertilizer and field condition control: Maintain rhizosphere pH between 6.0 and 7.0, and control soil moisture content at 60%-75% of field capacity, with longitudinal drainage in furrows.

[0060] The plant density for the Asteraceae strips is: 8–12 plants per m² for marigolds. -2The plant density of garland chrysanthemum is 12–16 plants per m. -2 Artemisia annua is 6–10 plants per m -2 The ginger rows are 70 cm wide, with a plant spacing of 20–25 cm; the Asteraceae crops (marigold + garland chrysanthemum) are planted in two alternating rows with a row spacing of 30 cm.

[0061] It is evident that the disease is suppressed to some extent, but the pathogen rebounds rapidly during the recovery period.

[0062] Results and Analysis: The data recorded from the above Examples 1, Comparative Examples 1-2, and Conventional Single Operation (CK) are as follows:

[0063] During the planting process, the incidence rate of ginger wilt disease in each group of ginger was measured, and the incidence rate was calculated as (number of diseased plants / total number of plants) × 100%. At the ginger harvest, the average plant height, stem diameter, and yield per acre were measured for each group of ginger. The results are shown in Table 1.

[0064] Table 1. Survey results of each embodiment and comparative example

[0065] Group Average plant height (cm) Average stem diameter (cm) Yield per mu (kg) Incidence rate of ginger wilt disease (%) Example 1 109.50±0.88 1.21±0.01 3131.33±5.24 18.89±0.38 Comparative Example 1 100.33±0.94 0.97±0.02 2863.67±5.56 37.56±0.83 Comparative Example 2 97.43±0.78 0.98±0.01 2811.67±4.19 38.00±0.94 CK 88.37±0.65 0.85±0.01 2631.33±5.44 44.44±0.83

[0066] As shown in Table 1, the ginger wilt integrated control technology of intercropping ginger with Asteraceae crops in strips and combining with biocontrol bacterial communities, as described in Example 1 of this invention, resulted in ginger plants with better average plant height, stem diameter, yield per acre, and ginger wilt incidence rate than Comparative Examples 1-2 and conventional monoculture (CK). Therefore, it can be seen that the ginger wilt integrated control technology of this invention can effectively promote the growth of ginger plants, reduce the incidence of ginger wilt, and thus increase the yield of ginger.

[0067] Example 1 of this invention utilizes root secretions and volatiles (such as thiothiophene and sesquiterpene lactones) from Asteraceae crops, along with the bio-fumigation effect of straw mowing, in synergistic effect with a Bacillus-Trichoderma-Pseudomonas / Streptomyces compound inoculant to significantly inhibit the colonization and migration of Ralstonia solanacearum. Field results show that compared with conventional monoculture controls, this invention can reduce the disease index by 40–70%, increase the yield of marketable ginger by 10–22%, and reduce the intensity of chemical pesticide use. This invention is compatible with existing drip irrigation, mulching, and high-ridge cultivation methods, and is suitable for large-scale application in high-humidity continuous cropping ecological niches such as Sichuan and Chongqing.

[0068] This invention is compatible with the existing ginger production system in terms of equipment and input. The marketability of crops (such as garland chrysanthemum, marigold cut flowers / fresh flowers) provides by-product income and can be promoted as a standardized ecological control module in continuous cropping and high humidity areas.

Claims

1. A method for integrated control of ginger wilt disease using intercropping of ginger and Asteraceae crops with synergistic biocontrol bacteria, characterized in that, Includes the following steps: S1. Pre-planting of Asteraceae crops: 15–30 days before ginger transplanting, make beds with a ratio of ginger rows to Asteraceae crop rows of (2–4):1, and plant Asteraceae crops in alternating rows of 1–2 rows on each strip. S2. Root dipping, seed soaking, or hole application of biocontrol agents: Biocontrol agents include liquid and solid inoculants. When transplanting ginger, use a concentration of 10... 7 -10 8 CFU·mL -1 The inoculant solution is used for root dipping or seed soaking; when applying in holes, the solid inoculant is mixed with the hole soil, and the mass ratio of the solid inoculant to the hole soil is (0.5–2.0):100; S3. Pre-flood harvesting and in-situ biological fumigation: 35–55 days after transplanting, before the main flood season, harvest the Asteraceae crops at a height of 10–20 cm from the ground, with a fresh weight of 3–6 t·ha. -1 Crush and cover the surface in place or shallowly till to a depth of 5–8 cm; S4. Drip irrigation with probiotics: 45–75 days after ginger transplanting, perform drip irrigation with probiotics 1–3 times at 7–14 day intervals, with each application containing ≥ 10¹¹ CFU·ha. -1 ; S5. Water, fertilizer and field condition control: Maintain rhizosphere pH between 6.0 and 7.0, and control soil moisture content at 60%-75% of field capacity. The furrows should have longitudinal drainage.

2. The integrated control method for ginger wilt disease based on ginger-Asteraceae intercropping and synergistic biocontrol bacteria as described in claim 1, characterized in that, The Asteraceae crops mentioned are at least one of marigold, garland chrysanthemum, artemisia, calendula, and sunflower.

3. The integrated control method for ginger wilt disease based on ginger-Asteraceae intercropping and synergistic biocontrol bacteria as described in claim 1, characterized in that, The plant density for the Asteraceae strips is: 8–12 plants per m² for marigolds. -2 The plant density of garland chrysanthemum is 12–16 plants per m. -2 Artemisia annua is 6–10 plants per m -2 The width of the ginger rows is 60–80 cm, and the spacing between the rows of Asteraceae plants is 25–35 cm.

4. The integrated control method for ginger wilt disease based on ginger-Asteraceae intercropping and synergistic biocontrol bacteria according to claim 1, characterized in that, The biocontrol bacteria include one or more of Bacillus, Trichoderma, Pseudomonas, and Streptomyces, and the viable count ratio of Bacillus:Trichoderma:Pseudomonas / Streptomyces is (2–5):(1–3):(0.5–2); the solid bacterial agent is a double-layer microcapsule or porous carrier adsorption type solid preparation with a water content ≤ 10% and a viable count ≥ 1×10⁻⁶. 8 CFU·g -1 .

5. The integrated control method for ginger wilt disease based on ginger-Asteraceae intercropping and synergistic biocontrol bacteria according to claim 4, characterized in that, The Bacillus species is any one of Bacillus velezensis, B. amyloliquefaciens, and B. subtilis; the Trichoderma species is any one of Trichoderma harzianum and T. asperellum; and the Pseudomonas species is any one of Pseudomonas chlororaphis and P. fluorescens. The bacterial suspension includes B. velezensis, T. harzianum, and P. chlororaphis, with a viable cell ratio of 3:1:

1. The solid bacterial agent is a composite bacterial strain obtained by combining B. velezensis, T. harzianum, and P. chlororaphis in a viable cell ratio of 3:1:

1. This composite bacterial strain is then loaded onto microporous zeolite / biochar to obtain particles with a diameter of 0.5–2.5 mm and a B. velezensis concentration ≥2 × 10⁻⁶. 9 CFU·g -1 The concentration of T. harzianum is ≥5×10 8 CFU·g -1 The concentration of P. chlororaphis is ≥1×10 9 CFU·g -1 The mass ratio of microporous zeolite to biochar is 1:

1.

6. The integrated control method for ginger wilt disease based on ginger-Asteraceae intercropping and synergistic biocontrol bacteria according to claim 1, characterized in that, Asteraceae crops should undergo a second height-limited harvesting 65–85 days after transplanting, yielding crops with a fresh weight of 2–4 t·ha. -1 Cover the furrows with Asteraceae crops to maintain ventilation, light, and the continuity of the "disease-suppressing zone"; complete a supplementary drip irrigation within 24–72 hours after mowing and biological fumigation to enhance the temporal synergy of decomposition and release of antibacterial bacteria.

7. The integrated control method for ginger wilt disease based on ginger-Asteraceae intercropping and synergistic biocontrol bacteria according to claim 1, characterized in that, When pre-sowing Asteraceae crops in S1, a boundary buffer zone should be set up, that is, marigolds should be planted around the perimeter of the plot with a width of 0.5–1.5 m and a density of 10–14 plants per m. -2 This forms a barrier to prevent the spread of diseases.

8. A supporting planting system for the integrated control of ginger wilt disease using the method of intercropping ginger with Asteraceae crops and synergistic biocontrol bacteria as described in any one of claims 1-7, characterized in that, This includes raised-ridge mulching cultivation, pre-sowing of Asteraceae crops, pre-harvesting and on-site biological fumigation before the flood season, and drip irrigation for bacterial supplementation; the drip irrigation tape is located 10–15 cm to the side of the ginger row root zone, and the Asteraceae crop rows are staggered at the outer edge of the mulch or inside the mulch holes; and an annual operation system consisting of two height-limited harvests, one shallow tillage, and 2–3 drip irrigations for bacterial supplementation.

9. The supporting planting system for the integrated control method of ginger wilt disease by intercropping ginger with Asteraceae crops and synergistic biocontrol bacteria as described in claim 8, characterized in that, The overlap rate of the canopy between the ginger rows and the rows of Asteraceae crops is 15–35%.