An apparatus for analyzing photosynthetic capacity of marine plants

Abstract

This invention discloses a marine plant photosynthetic capacity analysis device, applied in the field of marine ecological monitoring equipment technology. It includes: a photosynthetic chamber and an oxygen-secreting chamber, sharing a partition with a through hole; a first water-isolating component and a second water-isolating component. When the first water-isolating component is connected to the through hole, the photosynthetic chamber and the oxygen-secreting chamber are isolated; when the second water-isolating component is connected to the through hole, the leaves are located in the photosynthetic chamber and the roots are located in the oxygen-secreting chamber. The photosynthetic chamber is equipped with a light source. Both the photosynthetic chamber and the oxygen-secreting chamber are equipped with a heating device, a water inlet, and a liquid inlet. The water inlet is connected to a seawater supply pipeline, and the liquid inlet is selectively connected to a salinity adjustment solution supply pipeline or a pH adjustment solution supply pipeline; a detection device for detecting environmental parameters; and a control device configured to: before measuring the plant's photosynthetic capacity, adjust the water inlet flow rate and / or the liquid inlet flow rate of the light source, heating device, water inlet, and / or liquid inlet to restore the environmental parameters to a preset range. This improves the accuracy of marine plant photosynthetic capacity measurement.

Description

Technical Field

[0001] This invention relates to the field of marine ecological monitoring technology, and in particular to a device for analyzing the photosynthetic capacity of marine plants. Background Technology

[0002] Photosynthesis is a fundamental metabolic activity of large marine plants such as seagrass and algae, directly affecting their growth, development, reproduction, and physiological and ecological functions such as carbon sequestration. Evaluating the photosynthetic capacity of plants is crucial for exploring the adaptability of marine plants to environmental stresses and the differences in environmental adaptability among different species. Analytical indicators characterizing plant photosynthetic capacity include respiration rate, photosynthetic rate, root oxygen secretion, and the plant's ability and pathways for utilizing carbonates from seawater.

[0003] Different plants exhibit varying degrees of photosynthetic activity, which is also dynamically influenced by external environmental factors. Light intensity directly affects the rate of photosynthesis; stronger light generally increases the photosynthetic rate, while excessively high light intensity has an inhibitory effect. The photosynthetic capacity of marine plants can be comprehensively assessed by measuring four indicators: photosynthetic rate, respiration rate, light compensation point, and light saturation point. Photosynthetic rate and respiration rate characterize the plant's ability to produce photosynthetic output under light conditions and its ability to consume oxygen under shading conditions, respectively. The light compensation point and light saturation point characterize the plant's adaptability to shade-loving environments. Furthermore, root oxygen secretion indicators can characterize the plant's ecological restoration potential. In remediation and aquatic ecological environment restoration, utilizing the root oxygen secretion capacity of aquatic plants can increase the oxygen content in sediments, promoting the degradation and transformation of pollutants.

[0004] However, the various indicators reflecting the photosynthetic capacity of marine plants can change during the measurement process depending on environmental conditions. For example, changes in environmental parameters such as salinity, temperature, light intensity, and pH can all significantly affect the measurement results. Therefore, a stable and reliable seawater environment is required when analyzing the photosynthetic capacity of large marine plants.

[0005] In existing technologies, photosynthesis measuring instruments are mainly used for terrestrial plants, and their environmental adaptability is insufficient when applied to marine plants; portable underwater photosynthesis measuring systems lack effective sample fixation devices and real-time monitoring functions for environmental parameters; and dissolved oxygen microelectrode systems have defects such as limited functionality and insufficient environmental control.

[0006] Meanwhile, existing technologies typically measure various photosynthetic indicators individually, failing to perform combined analysis of multiple parameters. During the measurement of various indicators reflecting the photosynthetic capacity of marine plants, changes in environmental parameters such as salinity, temperature, light intensity, and pH directly affect the results. Therefore, there is an urgent need for an analytical device that combines these functions to improve the accuracy of photosynthetic capacity measurement in marine plants. Summary of the Invention

[0007] The purpose of this invention is to provide a device for analyzing the photosynthetic capacity of marine plants, which improves the accuracy of photosynthetic capacity measurement by controlling conditions such as temperature, pH, light, dissolved oxygen, salinity, and flow rate.

[0008] To solve the above-mentioned technical problems, the present invention provides a device for analyzing the photosynthetic capacity of marine plants, comprising:

[0009] The photosynthesis chamber and the oxygen secretion chamber are provided, with the oxygen secretion chamber located below the photosynthesis chamber. The bottom wall of the photosynthesis chamber and the top wall of the oxygen secretion chamber share the same partition, and the partition has a through hole.

[0010] The first water-proof component and the second water-proof component are provided with a through hole for the root and leaf connection of the plant to pass through;

[0011] The first waterproof component and the second waterproof component are selectively and sealingly connected within the through hole;

[0012] When the first water-proof component is connected to the through hole, the photosynthesis chamber and the oxygen secretion chamber are isolated from each other;

[0013] When the second water-proof component is connected to the through hole, the root-leaf junction of the plant is sealed through the through hole, so that the leaves of the plant are located in the photosynthetic chamber and the roots of the plant are located in the oxygen-secreting chamber.

[0014] The photosynthesis chamber is equipped with a light source. Both the photosynthesis chamber and the oxygen secretion chamber are equipped with a heating device, a water inlet, and a liquid inlet. The water inlet is connected to a seawater supply pipeline, and the liquid inlet is selectively connected to a salinity adjustment solution supply pipeline or a pH adjustment solution supply pipeline.

[0015] The marine plant photosynthetic capacity analysis device also includes:

[0016] The detection device is used to detect environmental parameters in the photosynthesis chamber and / or the oxygen secretion chamber in real time;

[0017] The control device is electrically connected to the detection device, the light source, the heating device, the water inlet, and the liquid inlet.

[0018] The control device is configured to, before the plant's photosynthetic capacity is measured, adjust the light intensity of the light source, the heating power of the heating device, the water inlet volume and / or the liquid inlet volume when the environmental parameters detected by the detection device deviate from the preset range, so as to restore the environmental parameters to the preset range.

[0019] Optionally, the second waterproof component includes at least two sector-shaped sub-plates, the radial sides of each sector-shaped sub-plate are joined together, and the inner arcs of each sector-shaped sub-plates together form the through hole;

[0020] The second waterproof component is embedded in the through hole, and the outer arc of the fan-shaped sub-plate is sealed and fitted with the inner peripheral wall of the through hole.

[0021] Optionally, the photosynthesis chamber and / or the oxygen secretion chamber are provided with a stirring impeller, the stirring impeller being electrically connected to the control device, the control device being configured to control the stirring impeller to operate intermittently or continuously during the measurement process.

[0022] Optionally, the detection device is provided in both the photosynthesis chamber and the oxygen secretion chamber. The detection device includes at least one set of sensor components, including a temperature sensor, an acid-base sensor, a light intensity sensor, a dissolved oxygen sensor, and a salinity sensor.

[0023] The sensor assembly is installed on the inner wall of the corresponding chamber;

[0024] The control device is located below the oxygen-secreting chamber, and the top wall of the control device and the bottom wall of the oxygen-secreting chamber are formed by the same support plate.

[0025] Optionally, the control device is configured to perform at least one of the following measurement modules:

[0026] The respiration measurement module controls the light source to turn off and the photosynthesis chamber to be shielded. After a preset dark adaptation time, the dissolved oxygen sensor detects the rate of change of dissolved oxygen and calculates the respiration rate.

[0027] The photosynthesis curve measurement module controls the light source to be adjusted step by step according to a preset light intensity gradient. After stabilizing for a preset time under each light intensity, the dissolved oxygen sensor detects the rate of change of dissolved oxygen and plots the photosynthetic response curve.

[0028] The root oxygen secretion measurement module controls the second water-proof component to be installed in the through hole, so that the plant roots are located in the oxygen secretion chamber, and detects the root oxygen secretion rate through the dissolved oxygen sensor in the oxygen secretion chamber.

[0029] The carbonate utilization capacity determination module controls the light source to maintain saturated light intensity, monitors the pH drift of the culture medium through the acid-base sensor, and determines the carbonate utilization capacity based on the pH compensation point.

[0030] Carbonate utilization pathway assay module: Controls the injection of inhibitors or buffer solutions into the inlet, compares the changes in photosynthetic rate before and after inhibition, and determines the contribution ratio of the extracellular carbonic anhydrase pathway or the proton pump pathway.

[0031] Optionally, it also includes:

[0032] A light-shielding cover is detachably fitted onto the outside of the photosynthesis chamber and the oxygen secretion chamber;

[0033] An annular protrusion is formed by extending radially outward from the outer edge of the bottom wall of the oxygen-secreting chamber, and the lower end face of the light shield abuts against the top surface of the annular protrusion.

[0034] Optionally, the heating device is a heat exchange pipe, which is arranged circumferentially around the inner wall of the photosynthesis chamber or the oxygen secretion chamber, and maintains a heat exchange gap with the inner wall surface of the corresponding chamber.

[0035] Optionally, the inner wall of the through hole is provided with a stepped surface, and the first water-proof component or the second water-proof component is supported on the stepped surface from top to bottom.

[0036] Optionally, it also includes:

[0037] A magnetic layer is laid on the top surface of the partition, as well as on the top surfaces of the first waterproof component and the second waterproof component;

[0038] The fastener includes a magnetic part that magnetically engages with the magnetic layer to fix the plant's leaves to the top surface of the partition.

[0039] Optionally, the fastener includes a cylindrical clamp and a U-shaped clamp, both of which are magnetically attracted to the magnetic layer.

[0040] The magnetic suction part of the cylindrical clamp is provided on its bottom end face for pressing and fixing the sheet-like blade;

[0041] The U-shaped clamp has a U-shaped cavity with its opening facing downwards. Its magnetic attraction part is located at the bottom of the two side walls of the opening, which is used to clamp and fix the thin strip blade. After the thin strip blade enters the U-shaped cavity through the opening, the magnetic attraction part of the U-shaped clamp is magnetically attracted to the magnetic attraction layer.

[0042] The marine plant photosynthetic capacity analysis device of the present invention has a through hole forming a vertical channel connecting the photosynthetic chamber and the oxygen secretion chamber. The first water-proof component is an integral sealing plate, and the second water-proof component has a through hole for the plant root-leaf connection to pass through. The two are selectively and sealed together in the through hole to achieve flexible switching of working modes.

[0043] When the first water-blocking component is connected to the through hole, its circumferential edge seals against the inner wall of the through hole, forming a complete seal that isolates the photosynthetic chamber from the oxygen-secreting chamber. The two chambers create independent, closed liquid environments, making the equipment suitable for measuring leaf photosynthetic capacity individually. When the second water-blocking component is connected to the through hole, the root-leaf junction is sealed through the through hole, placing the leaf portion in the photosynthetic chamber and the root portion in the oxygen-secreting chamber. This allows the leaves and roots of the same plant to be placed in different functional chambers while maintaining continuous physiological vascular tissue connection. The equipment can then perform leaf photosynthetic capacity measurements and root oxygen-secreting measurements to obtain overall plant photosynthetic capacity data.

[0044] When the environmental parameters detected by the detection device deviate from the preset range, the light intensity of the light source is adjusted by changing the output power or spectral composition of the light source to restore the light intensity to the preset range; the heating power of the heating device is adjusted by changing the heat output rate of the heating device to restore the temperature to the preset range; the water inlet flow rate is adjusted by injecting fresh seawater or discharging part of the liquid to restore the salinity to the preset range; and the liquid inlet flow rate is adjusted by injecting salinity adjusting solution or pH adjusting solution to restore the salinity or pH value to the preset range.

[0045] Compared to existing technologies, the advantages of this invention lie in the fact that the choice of a sealing connection method allows a single device to have multiple measurement modes, satisfying both the needs of individual leaf measurement and the needs of combined leaf and root measurement, significantly improving the device's versatility, measurement efficiency, and data integrity. By fixing live marine plants in the device, conditions such as light, temperature, and pH can be simultaneously controlled in a stable seawater environment to measure leaf and root respiration, leaf photosynthesis, carbonate utilization capacity and pathways, and root oxygen secretion. The device has a stable and reliable environmental control function, solving the problem of unstable external environment in the measurement of marine plant photosynthetic capacity and improving the accuracy of multiple indicator measurements. Attached Figure Description

[0046] To more clearly illustrate the technical solutions in the embodiments of the present invention or the prior art, the drawings used in the description of the embodiments or the prior art will be briefly introduced below. Obviously, the drawings described below are only embodiments of the present invention. For those skilled in the art, other drawings can be obtained based on the provided drawings without creative effort.

[0047] Figure 1 This is a schematic diagram of the overall structure of the marine plant photosynthetic capacity analysis device of the present invention;

[0048] Figure 2 This is a schematic diagram of the liquid-gas pipeline of the marine plant photosynthetic capacity analysis device of the present invention.

[0049] Figure 3This is a top view of the marine plant photosynthetic capacity analysis device of the present invention;

[0050] Figure 4 This is a schematic cross-sectional view of the photosynthesis chamber of the present invention;

[0051] Figure 5 This is a schematic diagram of the top cover of the present invention;

[0052] Figure 6 This is another view of the top cover of the present invention;

[0053] Figure 7 This is a schematic diagram of the structure of the partition of the present invention;

[0054] Figure 8 This is another view of the partition of the present invention;

[0055] Figure 9 This is a schematic diagram of the structure of the first water-proof component of the present invention;

[0056] Figure 10 This is another view of the first waterproof component of the present invention;

[0057] Figure 11 This is a schematic diagram showing the connection between the first water-proof component and the partition plate of the present invention;

[0058] Figure 12 This is a schematic diagram of the structure of the second water-proof component of the present invention;

[0059] Figure 13 This is another view of the second waterproof component of the present invention;

[0060] Figure 14 This is a schematic diagram showing the connection between the second water-proof component and the partition plate of the present invention;

[0061] Figure 15 This is a schematic diagram of the cylindrical chuck of the present invention;

[0062] Figure 16 This is a top view of the cylindrical clamp fixing blade part of the present invention;

[0063] Figure 17 This is a schematic diagram showing the placement of the rootless, leafless plant of the present invention in the equipment;

[0064] Figure 18 This is a schematic diagram showing the placement of a leafy plant in the equipment during the measurement of root oxygen secretion according to the present invention.

[0065] Figure 19 This is a schematic diagram of the structure of the U-shaped chuck of the present invention;

[0066] Figure 20 This is a top view of the U-shaped clamp fixing blade part of the present invention;

[0067] Figure 21 This is a schematic diagram showing the placement of the rootless columnar leaf plant of the present invention in the equipment;

[0068] Figure 22 This is a schematic diagram showing the placement of columnar leaf plants in the equipment during the measurement of root oxygen secretion according to the present invention.

[0069] Figure 23 This is a schematic diagram of the structure of the light shield of the present invention;

[0070] Figure 24 This is a schematic diagram illustrating the use of the light shield of the present invention;

[0071] Figure 25 This is a schematic diagram of the internal structure of the control device of the present invention;

[0072] Figure 26 This is a graph showing the fitting results of the photosynthetic curve of Tyrian lycoperdon persica per unit weight in this invention;

[0073] Figure 27 This is a graph showing the fitting result of the photosynthetic curve per unit area of ​​Tyrian grass according to the present invention;

[0074] Figure 28 This is a pH drift curve for determining the carbonic acid utilization capacity of needlegrass according to the present invention.

[0075] Explanation of reference numerals in the attached figures:

[0076] 1. Top cover; 2. Rotary handle; 3. Light source; 4. Transparent lampshade; 5. Image acquisition device; 6. Photosynthesis chamber; 7. Stirring impeller; 8. Heat exchange pipe; 81. Heat sink; 9. Oxygen secretion chamber; 10. Dissolved oxygen sensor; 11. Acid-base sensor; 12. Salinity sensor; 13. Temperature sensor; 14. Light intensity sensor; 15. Partition; 151. Magnetic layer; 152. Through hole; 153. Stepped surface; 16. Bubble level; 17. Support plate; 171. Light leakage prevention protrusion; 172. Light leakage prevention groove ; 18 Control device; 181 Display screen; 182 Operation keyboard; 183 Electrical control module; 184 Circulating water temperature control device; 185 Peristaltic pump; 19 Lifting foot; 20 First water-proof component; 21 Second water-proof component; 211 Through hole; 22 Sealing gasket; 23 Cylindrical chuck; 24 U-shaped chuck; 25 Magnetic suction part; 26 Light shield; 261 Observation hole; 262 Baffle; 27 Liquid inlet; 28 Water inlet; 29 Air outlet; 30 Water outlet; 31 Water inlet pump. Detailed Implementation

[0077] The core of this invention is to provide a device for analyzing the photosynthetic capacity of marine plants, which improves the accuracy of photosynthetic capacity measurement by controlling conditions such as temperature, pH, light, dissolved oxygen, salinity, and flow rate.

[0078] The technical solutions of the embodiments of the present invention will be clearly and completely described below with reference to the accompanying drawings. Obviously, the described embodiments are only some embodiments of the present invention, and not all embodiments. Based on the embodiments of the present invention, all other embodiments obtained by those skilled in the art without creative effort are within the scope of protection of the present invention.

[0079] In one specific embodiment provided by the present invention, please refer to Figures 1-25 The marine plant photosynthetic capacity analysis equipment mainly includes a photosynthesis chamber 6, an oxygen secretion chamber 9, a first water-proof component 20, and a second water-proof component 21.

[0080] The photosynthesis chamber 6 and the oxygen secretion chamber 9 are located below the photosynthesis chamber 6. The bottom wall of the photosynthesis chamber 6 and the top wall of the oxygen secretion chamber 9 share the same partition 15. A through hole 152 is provided on the partition 15.

[0081] The first water-proof component 20 and the second water-proof component 21 are provided with a through hole 211 for the plant root and leaf connection part to pass through.

[0082] The first waterproof component 20 and the second waterproof component 21 are selectively and sealingly connected within the through hole 152;

[0083] When the first water-proof component 20 is connected to the through hole 152, the photosynthesis chamber 6 and the oxygen secretion chamber 9 are isolated from each other;

[0084] When the second water-proof component 21 is connected to the through hole 152, the root-leaf connection of the plant is sealed through the through hole 211, so that the leaf part of the plant is located in the photosynthesis chamber 6 and the root part of the plant is located in the oxygen secretion chamber 9.

[0085] It should be noted that the photosynthesis chamber 6 and the oxygen excretion chamber 9 constitute the core cavity structure of the equipment. The oxygen excretion chamber 9 is located below the photosynthesis chamber 6, forming a double-layered layout. The bottom wall of the photosynthesis chamber 6 and the top wall of the oxygen excretion chamber 9 are formed by the same partition 15. This partition 15, as an integrally formed intermediate partition structure, serves both as a physical barrier separating the two functional chambers and as a transitional carrier connecting the two chambers. The thickness of the partition 15 is designed according to structural strength and sealing requirements. Its upper and lower surfaces respectively form the bottom interface of the photosynthesis chamber 6 and the top interface of the oxygen excretion chamber 9, ensuring clear spatial definition of the two chambers in the vertical direction. A through hole 152 is provided on the partition 15, which completely penetrates along the thickness direction of the partition 15, forming a vertical channel connecting the photosynthesis chamber 6 and the oxygen excretion chamber 9. The inner wall contour of the through hole 152 is adapted to the outer contour of the first water-proof component 20 and the second water-proof component 21, providing a mating basis for the sealed installation of the water-proof components.

[0086] The first water-sealing component 20 and the second water-sealing component 21 are two complementary sealing components. One of them is selectively and sealingly connected within the through-hole 152, allowing for flexible switching of the equipment's operating mode by replacing different water-sealing components. The first water-sealing component 20 is an integral sealing plate structure, its outer contour matching the inner wall contour of the through-hole 152. When the first water-sealing component 20 is connected to the through-hole 152, its circumferential edge seals against the inner wall of the through-hole 152, forming a complete seal from the top to the bottom of the partition 15, isolating the photosynthesis chamber 6 from the oxygen-secreting chamber 9. At this time, the two chambers form their own independent and closed liquid environments. The measurement operations in the photosynthesis chamber 6 are completely isolated from the oxygen-secreting chamber 9, avoiding liquid exchange, gas interference, or temperature mutual influence between the upper and lower chambers. This method is suitable for measuring the respiration of marine plants, the photosynthetic curve of marine plant leaves, and the carbonate utilization capacity and pathways of marine plants within the photosynthesis chamber 6.

[0087] The second water-proof component 21 has a through hole 211 through which the root-leaf junction of the plant passes. This through hole 211 extends along the thickness direction of the second water-proof component 21, and its diameter is designed according to the typical size range of the root-leaf junction of the plant under test. When the second water-proof component 21 is connected to the through hole 152, the circumferential edge of the second water-proof component 21 seals against the inner wall of the through hole 152. The root-leaf junction of the plant passes downwards from the photosynthetic chamber 6 through the through hole 211 and extends into the oxygen-secreting chamber 9. A sealed contact is formed between the inner wall of the through hole 211 and the outer surface of the root-leaf junction, ensuring that the root-leaf junction passes through the through hole 211 in a sealed manner. The gap between the through hole 211 and the root-leaf junction can be sealed by filling with molten paraffin wax. This sealed configuration places the plant's leaves in the photosynthesis chamber 6 and the roots in the oxygen-secreting chamber 9. The root-leaf junction, acting as a transition section, is fixed in the through-hole 211 of the second water-proof component 21. This allows the leaves and roots of the same plant to be placed in different functional chambers while maintaining continuous physiological vascular tissue connection. The equipment then measures leaf photosynthesis and root oxygen secretion. The photosynthetic products produced by the leaves in the photosynthesis chamber 6 are transported to the roots through the vascular tissue. The oxygen released by the roots during respiration in the oxygen-secreting chamber 9 can be measured independently. This provides data on the overall photosynthetic capacity of the plant and dynamic information on the source-sink relationship, making it suitable for measuring root oxygen secretion in marine plants.

[0088] The selective sealing connection between the first water-proof component 20 and the second water-proof component 21, based on the same through-hole 152 structure, allows for quick replacement, enabling switching of operating modes without altering other parts of the equipment. The sealing connection between both water-proof components and the through-hole 152 employs a detachable mechanical fit, facilitating cleaning, maintenance, and replacement. This modular design allows a single device to support multiple measurement modes, meeting both individual leaf measurement needs and combined leaf and root measurement requirements. This significantly improves the equipment's versatility, measurement efficiency, and data integrity, providing a reliable hardware foundation for the comprehensive evaluation of marine plant photosynthetic capacity.

[0089] The photosynthesis chamber 6 is equipped with a light source 3, which serves as the energy source for photosynthesis, providing controllable light conditions for the plant leaves. The light intensity of the light source 3 can be adjusted according to the measurement requirements to simulate different natural light environments or construct specific light response curves for gradient measurement. Both the photosynthesis chamber 6 and the oxygen secretion chamber 9 are equipped with heating devices, water inlets 28, and liquid inlets 27. The environmental control systems of the two chambers are independently configured, allowing for differentiated parameter settings according to their respective measurement needs. The heating devices are used to regulate the temperature of the liquid within the chambers, maintaining it within a range suitable for the physiological activities of marine plants and ensuring that the plants are in a normal metabolic state during the measurement process. The water inlet 28 is connected to a seawater supply pipeline, used to add natural seawater or artificially prepared seawater culture medium to the photosynthesis chamber 6 and the oxygen secretion chamber 9, providing the plants with the necessary liquid environment and inorganic nutrients. The inlet 27 can be selectively connected to either the salinity adjustment solution supply line or the pH adjustment solution supply line. By switching between different supply lines, salinity adjustment solution or pH adjustment solution can be selectively injected into the chamber to achieve precise control of seawater salinity or acidity / alkalinity, meeting the environmental requirements of different marine plant species or different experimental treatments.

[0090] The marine plant photosynthetic capacity analysis equipment also includes a detection device and a control device 18, forming a complete integrated measurement and control system. The detection device is used to monitor environmental parameters in the photosynthesis chamber 6 and / or the oxygen secretion chamber 9 in real time. Depending on the measurement mode, it can monitor environmental parameters in the photosynthesis chamber 6 alone, the oxygen secretion chamber 9 alone, or both chambers simultaneously, achieving flexible monitoring configuration. The environmental parameters detected by the detection device include physicochemical indicators closely related to the photosynthetic capacity of marine plants, such as temperature, salinity, and pH, providing a data basis for environmental control and measurement recording. The control device 18 is electrically connected to the detection device, the light source 3, the heating device, the water inlet 28, and the liquid inlet 27, establishing a bidirectional path for signal acquisition and execution control. The detection device transmits the real-time collected environmental parameter data to the control device 18. The control device 18 makes judgments and decisions based on preset control logic and parameter thresholds, and issues control commands to the light source 3, the heating device, the water inlet 28, and the liquid inlet 27, forming a closed-loop automatic control circuit.

[0091] The control device 18 includes a display screen 181, an operation keyboard 182, an electronic control module 183, a circulating water temperature control device 184, and a peristaltic pump 185. The inlet 28 is connected to the peristaltic pump 185 via the inlet pump 31, enabling precise liquid delivery and flow control. The display screen 181 displays environmental parameters, measurement progress, and results in real time. The operation keyboard 182 is used to input control commands and parameter settings. The electronic control module 183 is the core processing unit of the control device 18, electrically connected to the light source 3, heating device, detection device, stirring impeller 7, circulating water temperature control device 184, image acquisition device 5, peristaltic pump 185, and temperature sensor 13, acid-base sensor 11, light intensity sensor 14, dissolved oxygen sensor 10, and salinity sensor 12, enabling signal acquisition, logical judgment, and command output. The circulating water temperature control device 184 is connected to the heat exchange pipeline 8, achieving dynamic temperature control through the circulating heat exchange medium. The peristaltic pump 185 controls the inlet pump 31 to precisely regulate the amount of water entering the photosynthesis chamber 6 and the oxygen secretion chamber 9.

[0092] Control device 18 is configured to perform an environmental pre-regulation program before the plant's photosynthetic capacity is measured. When the environmental parameters detected by the detection device deviate from the preset range, control device 18 activates the corresponding regulation mechanism: regulating the light intensity of light source 3 by changing the output power or spectral composition of light source 3 to restore the light intensity to the preset range; regulating the heating power of the heating device by changing the heat output rate of the heating device to restore the temperature to the preset range; regulating the water inlet 28 by injecting fresh seawater or discharging part of the liquid to restore the salinity to the preset range; and regulating the liquid inlet 27 by injecting salinity adjustment solution or pH adjustment solution to restore the salinity or pH value to the preset range. The above regulation operations can be performed individually or in combination until the environmental parameters are restored to the preset range, ensuring that the environmental conditions in the chamber are stable and reliable at the start of the measurement, providing environmental assurance for the accuracy and comparability of the photosynthetic capacity measurement data.

[0093] Compared to existing technologies, the marine plant photosynthetic capacity analysis device of this invention is suitable for laboratory environments. Live marine plants are immobilized within the device, and a stable seawater environment is ensured by controlling environmental parameters such as temperature, pH, light, dissolved oxygen, and salinity. The device simultaneously measures multiple functions, including photosynthesis, respiration, carbonate utilization capacity and pathways, and root oxygen secretion, achieving integrated measurement of photosynthetic capacity-related indicators. The device possesses a stable and reliable environmental regulation function for plant photosynthesis, solving the problem of unstable external environments affecting leaf and root measurements in marine plant photosynthetic capacity determination, and significantly improving the accuracy of multiple indicators related to seagrass photosynthetic capacity.

[0094] In one specific embodiment, the second water-proof component 21 adopts a split-type assembly structure, including at least two fan-shaped sub-plates. Each fan-shaped sub-plate is a plate-shaped component with a fan-shaped outline, and its radial side is a straight edge along the radial direction. The radial sides of adjacent fan-shaped sub-plates are spliced ​​together to form a complete circle or near-circular whole. The inner arc of each fan-shaped sub-plate is an arc-shaped edge close to the center of the circle. The inner arcs of multiple fan-shaped sub-plates together form a through hole 211, which is located in the central area of ​​the second water-proof component 21 and is used for the plant root-leaf connection part to pass through. The outer arc of each fan-shaped sub-plate is an arc-shaped edge away from the center of the circle, and its curvature is adapted to the inner peripheral wall curvature of the through hole 152. When each fan-shaped sub-plate is assembled and embedded in the through hole 152, the outer arc of the fan-shaped sub-plate and the inner peripheral wall of the through hole 152 form a sealed fit, realizing the circumferential seal between the second water-proof component 21 and the through hole 152.

[0095] The modular design of the second waterproof component 21 makes the installation of the root-leaf connection more convenient. During installation, the root-leaf connection of the plant is first placed in the through hole 152 of the partition 15. Then, the fan-shaped sub-plates are sequentially assembled from different circumferential positions of the root-leaf connection, so that the root-leaf connection is enclosed in the through hole 211 formed by the inner arc of each fan-shaped sub-plate. Finally, the assembled second waterproof component 21 is completely embedded into the through hole 152 for fixation. This installation method avoids the difficulty of inserting the root-leaf connection from the end into a closed hole, and is particularly suitable for marine plants with well-developed root systems or irregular root-leaf connection shapes. For disassembly, the fan-shaped sub-plates can be removed sequentially by reversing the operation, facilitating the removal of the plant and the cleaning and maintenance of the second waterproof component 21.

[0096] The number of sector-shaped sub-plates is determined based on the size of the root-leaf connection and ease of installation. At least two sector-shaped sub-plates can achieve basic splicing functionality. The splicing of multiple sector-shaped sub-plates can make the edge of the through hole 211 formed by the inner arc smoother, reducing mechanical damage to the root-leaf connection. The radial side splicing surfaces of the sector-shaped sub-plates can be designed as mutually cooperating concave-convex structures or planar structures. After splicing, adjacent sector-shaped sub-plates form a tight contact, preventing liquid leakage from the splicing gaps. The sealing fit between the outer arc of the sector-shaped sub-plate and the inner peripheral wall of the through hole 152 can be achieved through sealing gaskets 22 or precision machining, ensuring the connection sealing between the second water-proof component 21 and the partition 15, preventing liquid interpenetration between the photosynthetic chamber 6 and the oxygen-secreting chamber 9, and ensuring accurate measurement of the independent environmental parameters of the two chambers.

[0097] In one specific embodiment, both the photosynthesis chamber 6 and the oxygen secretion chamber 9 are equipped with detection devices. The detection devices in the two chambers are independently configured, allowing for real-time monitoring of environmental parameters within each chamber, thus supporting simultaneous data measurement in both chambers. The detection device includes at least one set of sensor components, each set being an integrated multi-parameter sensing unit encompassing multiple sensing elements such as a temperature sensor 13, an acid-base sensor 11, a light intensity sensor 14, a dissolved oxygen sensor 10, and a salinity sensor 12. The temperature sensor 13 detects the temperature of the liquid, providing feedback signals for the control of the heating device. The acid-base sensor 11 detects the acidity or alkalinity of the liquid, reflecting the equilibrium state of the carbonate system and the degree of photosynthesis. The light intensity sensor 14 detects the light intensity within the photosynthesis chamber 6, verifying the consistency between the output of the light source 3 and the set value. The dissolved oxygen sensor 10 detects the concentration of dissolved oxygen in the liquid, providing a direct basis for calculating the photosynthetic rate and respiration rate. The salinity sensor 12 detects the salinity of the liquid, reflecting the osmotic pressure environment of seawater. The aforementioned sensors work together to comprehensively cover key environmental parameters for measuring the photosynthetic capacity of marine plants.

[0098] The sensor assembly is mounted on the inner wall of the corresponding chamber, with the sensor's sensitive element in direct contact with the liquid inside, ensuring the accuracy and real-time nature of the detection data. The installation location avoids the working area of ​​the stirring impeller 7 and the area where plants are placed, preventing mechanical interference and biological obstruction from affecting the detection results. The placement height of the sensor assembly is determined based on the typical location of the object being measured and the liquid depth, ensuring the sensor is positioned in a representative liquid layer.

[0099] The control device 18 is located below the oxygen-exuding chamber 9, forming the core control area at the bottom of the equipment. The top wall of the control device 18 and the bottom wall of the oxygen-exuding chamber 9 are constructed from the same support plate 17. This support plate 17 serves as a structural transition component, supporting the weight of the oxygen-exuding chamber 9 and its internal components upwards, and enclosing the top space of the control device 18 downwards, thus integrating structural functions. The material of the support plate 17 balances structural strength and electromagnetic shielding requirements, providing a stable mounting base and protective environment for the electronic components within the control device 18. The control device 18, together with the photosynthesis chamber 6 and the oxygen-exuding chamber 9 above, forms a vertically stacked overall layout, shortening the routing of electrical connection lines, reducing signal transmission interference and loss, and improving the system's integration and reliability.

[0100] In a preferred embodiment, a bubble level 16 is provided on the support plate 17. The bubble level 16 is a tubular indicating device with built-in liquid bubbles. By observing the position of the bubbles relative to the scale lines, the horizontal state of the support plate 17 can be displayed intuitively, providing a visual reference for the leveling operation of the equipment.

[0101] The bottom wall of the control device 18 is provided with lifting feet 19. The lifting feet 19 include a threaded rod and a support cap. The threaded rod is a columnar rod with external threads, and the support cap is a disc-shaped or bowl-shaped base with anti-slip texture. The threaded rod is threadedly connected to the support plate 17. By rotating the threaded rod to change its screw depth, the height of each corner of the support plate 17 can be independently adjusted to adjust the overall level of the equipment.

[0102] The coordinated design of the bubble level 16 and the lifting foot 19 makes the horizontal adjustment of the equipment visible and precise, ensuring that the liquid level and sensor position are accurate during the measurement process, and eliminating liquid level differences and measurement errors caused by equipment tilt.

[0103] In a preferred embodiment, the top wall of the photosynthesis chamber 6 is formed by a top cover 1, which is an openable cover-like structure and is detachably and sealingly connected to the top of the side wall of the photosynthesis chamber 6, forming a closed top of the photosynthesis chamber 6. Exemplarily, the top cover 1 is threaded or snap-fitted to the top of the side wall of the photosynthesis chamber 6. The threaded connection achieves tight fixation through rotational engagement, while the snap-fit ​​connection achieves quick assembly and disassembly through elastic engagement. Both connection methods facilitate the opening and closing of the top cover 1. A sealing ring is provided at the connection point. This sealing ring is an annular sealing element made of elastic material, filling the mating gap between the top cover 1 and the top of the side wall. When the connection is locked, it is compressed and deformed to form a reliable static seal, preventing liquid leakage from the connection point. A rotating handle 2 may be provided on the top cover 1. This rotating handle 2 is a grip structure protruding from the surface of the top cover 1, facilitating the operator's grip and application of force. The rotating handle 2 is used to drive the top cover 1 to rotate relative to the photosynthesis chamber 6 to lock or unlock. In the threaded connection method, the rotation of the rotating handle 2 drives the top cover 1 to rise and fall in a spiral manner to achieve connection or separation. In the snap-fit ​​connection method, the rotation of the rotating handle 2 drives the top cover 1 to move circumferentially to achieve snap engagement or disengagement.

[0104] A light source 3 is provided on the side of the top cover 1 facing the photosynthesis chamber 6. This light source 3 serves as the energy source for photosynthesis, providing controllable light conditions for the plant leaves. The light source 3 is preferably an adjustable spectrum LED light source, a surface light source 3 composed of an array of light-emitting diodes, or a point light source 3. The spectral composition and light intensity can be adjusted by the control device 18 to meet the light quality and quantity requirements of different photosynthesis measurements. A transparent lampshade 4 may be provided on the top cover 1. This transparent lampshade 4 is a shell structure made of light-transmitting material. The light source 3 is housed inside the transparent lampshade 4. The transparent lampshade 4 provides physical protection for the light source 3, preventing damage to the light source 3 from liquid splashes or mechanical collisions, while allowing light to pass through into the photosynthesis chamber 6 without obstruction, without affecting the lighting effect.

[0105] The top cover 1 is also equipped with an image acquisition device 5, such as a camera. The image acquisition device 5 is connected to the image processing module of the control device 18 and transmits image data through wired or wireless means. It is used to collect and transmit the morphology and color image data of the plant leaves in real time, so as to realize non-contact measurement of leaf area and dynamic monitoring of leaf condition.

[0106] In a preferred embodiment, a stirring impeller 7 is provided in the photosynthesis chamber 6 and / or the oxygen secretion chamber 9. The stirring impeller 7 is installed at the bottom or side wall of the chamber, with its blades partially immersed in the liquid. The stirring impeller 7 is electrically connected to a control device 18, which is configured to control the stirring impeller 7 to operate intermittently or continuously during the measurement process. In the intermittent operation mode, stirring is initiated at a specific time point to eliminate liquid stratification and local concentration differences, and then stopped, reducing interference with the dissolved oxygen sensor 10. In the continuous operation mode, the impeller 7 maintains a low-speed rotation throughout the measurement process to maintain a uniform mixing state of the liquid. The operation of the stirring impeller 7 ensures a uniform liquid environment within the chamber, ensuring that the sensor readings represent the true state of the overall liquid, avoiding measurement errors caused by local temperature, pH, or dissolved oxygen differences, and improving the accuracy and reproducibility of the data.

[0107] In one specific embodiment, the control device 18 is configured to execute at least one of the following measurement modules, each corresponding to a different marine plant photosynthetic capacity analysis function, and to achieve automated measurement through the programmed control of the control device 18.

[0108] The respiration measurement module is used to determine the respiration rate of plants under dark conditions. This module controls the light source 3 to turn off and simultaneously controls the shading of the photosynthetic chamber 6, creating a completely dark measurement environment. After a preset dark adaptation time, photosynthesis in the plant completely ceases, and respiration consumes dissolved oxygen. The rate of decrease in dissolved oxygen concentration is detected by the dissolved oxygen sensor 10, and the respiration rate is calculated. The set dark adaptation time ensures that the plant fully transitions from a light-exposed state to a dark state, eliminating the influence of interfering factors such as photorespiration. This module enables the equipment to measure dark respiration, improves the acquisition of basic metabolic parameters for evaluating photosynthetic capacity, and provides respiration consumption data for calculating the net photosynthetic rate.

[0109] The photosynthetic curve measurement module is used to determine the light response characteristics of plants. This module controls the light source 3 to adjust stepwise according to a preset light intensity gradient, starting from low light intensity and gradually increasing to high light intensity, covering the complete range from the light compensation point to the light saturation point. After stabilizing for a preset time at each light intensity, the plant's photosynthesis reaches a steady state. The rate of increase in dissolved oxygen concentration is detected by the dissolved oxygen sensor 10, and the net photosynthetic rate at each light intensity is calculated, thereby plotting the photosynthetic response curve. This module enables the equipment to automatically map light response characteristics, obtaining complete photosynthetic parameters such as the light compensation point, light saturation point, and maximum net photosynthetic rate in a single measurement, avoiding the cumbersome operation of repeatedly changing the light source 3 in traditional methods.

[0110] The root oxygen secretion measurement module is used to determine the oxygen secretion capacity of plant roots. This module controls the installation of the second water-blocking component 21 within the through-hole 152, positioning the plant roots in the oxygen secretion chamber 9 and the leaves in the photosynthetic chamber 6. The rate of increase in dissolved oxygen concentration caused by root oxygen release is detected by the dissolved oxygen sensor 10 within the oxygen secretion chamber 9, and the root oxygen secretion rate is calculated. This module enables the simultaneous measurement of leaf photosynthesis and root oxygen secretion, revealing the relationship between overall photosynthetic carbon fixation and root oxygen release in the plant, providing data support for evaluating the ecological restoration potential and rhizosphere microecological effects of marine plants.

[0111] The carbonate utilization capacity measurement module is used to determine the plant's ability to utilize bicarbonate ions from seawater. This module controls light source 3 to maintain saturated light intensity, ensuring continuous inorganic carbon consumption during photosynthesis. pH fluctuations in the culture medium are monitored by acid-base sensor 11. As photosynthesis progresses, the pH gradually rises until it stabilizes. The carbonate utilization capacity is determined based on the final pH compensation point. A pH compensation point above a specific threshold indicates that the plant has the ability to effectively utilize bicarbonate ions from seawater. This module enables the equipment to determine inorganic carbon utilization strategies, distinguishing between species that can utilize free carbon dioxide and those that can utilize bicarbonate ions, providing physiological indicators for research on the ecological adaptability of marine plants.

[0112] The carbonate utilization pathway assay module is used to elucidate the specific physiological pathways by which plants utilize bicarbonate. This module controls the injection of either an inhibitor or a buffer solution through inlet 27. The inhibitor can be acetazolamide, used to specifically inhibit the extracellular carbonic anhydrase pathway, while the buffer solution can be triaminomethane hydrochloride, used to neutralize hydrogen ions generated by the proton pump pathway. The changes in photosynthetic rate before and after inhibition are compared, and the contribution ratio of each pathway to bicarbonate utilization is calculated. This module enables the instrument to perform fine-grained analysis of carbon concentration mechanisms, revealing differences in inorganic carbon acquisition strategies among different marine plant species or even the same species under different environmental conditions, providing mechanistic evidence for understanding the evolutionary adaptation of photosynthesis in marine plants.

[0113] The integrated design of the aforementioned measurement modules enables a single device to comprehensively evaluate the photosynthetic capacity of marine plants, forming a complete analytical system from basic photosynthetic parameters to carbon metabolism mechanisms, and from leaf function to root effects. Each module is automated through programmed calls from the control device 18, eliminating the need for manual intervention during the measurement process. This improves measurement efficiency, data accuracy, and experimental repeatability, providing an efficient and reliable technical platform for the physiological and ecological research of marine plants.

[0114] In a preferred embodiment, the control device 18 is further configured to execute a pretreatment module before the root oxygen secretion measurement module is activated. This pretreatment module controls the inlet 27 to inject sodium sulfite solution into the oxygen secretion chamber 9. Sodium sulfite, as a chemical oxygen remover, reacts with dissolved oxygen in the liquid within the oxygen secretion chamber 9 via a redox reaction, consuming and removing the dissolved oxygen, thereby reducing the background dissolved oxygen level in the oxygen secretion chamber 9.

[0115] The determination of root oxygen secretion rate relies on detecting the increase in dissolved oxygen concentration caused by root oxygen release. If the background dissolved oxygen value in the oxygen secretion chamber 9 is too high, the concentration change caused by root oxygen secretion is relatively small, reducing the sensitivity and accuracy of the detection signal. By injecting sodium sulfite solution to lower the background dissolved oxygen value, the initial dissolved oxygen concentration is kept at a low level, making the concentration increase caused by root oxygen secretion more significant, thus improving the detection sensitivity and data resolution of the dissolved oxygen sensor 10.

[0116] The injection volume of sodium sulfite solution is controlled based on the volume of the oxygen secretion chamber 9 and the target background value. After injection, the solution is thoroughly mixed and reacted. The root oxygen secretion measurement module is then activated only after the dissolved oxygen concentration has stabilized within the target range. This pretreatment module is automatically executed by the control device 18, ensuring the standardization of measurement conditions and the comparability of results.

[0117] In one specific embodiment, the marine plant photosynthetic capacity analysis device also includes a light shield 26 and an annular boss, which together form a detachable light shielding system to provide complete light protection for the measurement module that requires a dark environment.

[0118] The light shield 26 is an opaque, dome-shaped structure that is detachably fitted onto the outside of the photosynthesis chamber 6 and the oxygen secretion chamber 9, covering the side and top areas of both chambers to block external light from entering. The lower end of the light shield 26 is open and engages with an annular protrusion for positioning and support. The top of the light shield 26 can be designed as a closed end or equipped with an openable top cover 1 for easy observation or operation while in the shaded state. The light shield 26 is made of opaque flexible or rigid material; flexible material facilitates folding and storage and adapts to the shape of the equipment, while rigid material helps maintain a stable shaded form.

[0119] The annular boss extends radially outward from the outer edge of the bottom wall of the oxygen-exuding chamber 9, forming a horizontal annular flange structure. The top surface of the annular boss serves as a support surface, and the lower end face of the light-shielding cover 26 abuts against the top surface of the annular boss, achieving axial positioning and vertical support for the light-shielding cover 26. This abutment seals the opening end of the light-shielding cover 26 with the annular boss, forming a continuous light-shielding space extending upward from the annular boss to the top of the light-shielding cover 26, enclosing the photosynthesis chamber 6 and the oxygen-exuding chamber 9 in a dark environment. The radial width of the annular boss is designed according to the wall thickness of the light-shielding cover 26 and the fitting requirements, ensuring sufficient contact area between the lower end face of the light-shielding cover 26 and the top surface of the annular boss, improving support stability and light-shielding sealing.

[0120] The detachable connection between the light shield 26 and the annular boss allows for quick and easy installation and removal of the light-shielding system. When performing photosynthesis measurements requiring illumination, removing the light shield 26 exposes the photosynthesis chamber 6 to the light source 3; when performing respiration measurements or dark adaptation treatments requiring darkness, quickly attaching the light shield 26 provides complete shading. This design enhances the flexibility of equipment operation and the continuity of the measurement process, allowing for switching between light and dark conditions without moving equipment or changing the measurement site.

[0121] In a preferred embodiment, the side wall of the light shield 26 is provided with an observation hole 261. The observation hole 261 is a through-hole structure that penetrates the side wall of the light shield 26, used for observing the internal condition of the equipment or performing simple operations when the light shield is in a light-shielding state. A baffle 262 is hinged to the observation hole 261. One edge of the baffle 262 is connected to the light shield 26 via a hinge, and the baffle 262 can be opened and closed relative to the observation hole 261. In the closed state, the baffle 262 covers the observation hole 261, maintaining the complete light-shielding performance of the light shield 26; in the open state, the baffle 262 flips outward around the hinge, exposing the observation hole 261, making it convenient for operators to observe the plant condition or read simple indicators. This configuration allows the light shield 26 to maintain its overall light-shielding function while also providing local observation capabilities, avoiding the disturbance to the measurement environment caused by frequent disassembly and reassembly of the light shield 26.

[0122] In a preferred embodiment, the top surface of the annular boss is coaxially provided with a light-leakage prevention protrusion 171 and a light-leakage prevention groove 172, with the light-leakage prevention protrusion 171 located inside the light-leakage prevention groove 172, forming a double-ring concentric structure consisting of the light-leakage prevention protrusion 171 and the light-leakage prevention groove 172 arranged sequentially from the center outwards. The light-leakage prevention protrusion 171 is an annular rib that protrudes upwards along the top surface of the annular boss, and the light-leakage prevention groove 172 is an annular groove that is recessed downwards along the top surface of the annular boss. Together with the top surface of the annular boss, the two constitute an alternating concave-convex light-leakage prevention interface.

[0123] The lower edge of the light-shielding cover 26 is inserted into the light-leakage prevention groove 172 and engages with it. The lower edge of the light-shielding cover 26 has a thin-walled edge structure, the thickness and contour of which are adapted to the width and depth of the light-leakage prevention groove 172, forming a tight fit after insertion. This engagement achieves radial positioning and axial fixation of the lower edge of the light-shielding cover 26, preventing horizontal displacement and tipping risks. At the same time, the groove wall of the light-leakage prevention groove 172 circumferentially wraps around the lower edge of the light-shielding cover 26, blocking external light from entering through the gap between the lower end of the light-shielding cover 26 and the annular protrusion. Together with the inner light-leakage prevention protrusion 171, it forms multiple light-shielding barriers, significantly improving the light-leakage prevention performance of the light-shielding system.

[0124] In a preferred embodiment, the cross-sections of the light-leakage-preventing protrusion 171 and the light-leakage-preventing groove 172 are trapezoidal, arc-shaped, or rectangular. The trapezoidal cross-section has a guiding bevel, facilitating quick insertion and positioning of the lower edge of the light-shielding cover 26; the arc-shaped cross-section eliminates stress concentration and improves structural durability; the rectangular cross-section is easy to process and has high fitting accuracy. The three cross-sectional shapes can be selected based on the processing technology, fitting accuracy, and frequency of use, all achieving good light-leakage prevention and structural reliability.

[0125] In one specific embodiment, the heating device is a heat exchange pipe 8, which has a hollow tubular structure with a heat exchange medium flowing inside. It exchanges heat with the external liquid through the pipe wall to heat or maintain the temperature of the liquid within the chamber. The heat exchange pipe 8 is arranged circumferentially around the inner wall of the photosynthetic chamber 6 or the oxygen-secreting chamber 9, forming a spiral or annular path around the inner wall of the chamber. This ensures that the heat exchange area covers the circumferential range of the chamber, guaranteeing uniform heat distribution. A heat exchange gap is maintained between the heat exchange pipe 8 and the inner wall of the corresponding chamber. This gap is the radial distance between the outer wall of the heat exchange pipe 8 and the inner wall of the chamber. This gap avoids direct contact between the heat exchange pipe 8 and the inner wall of the chamber, preventing localized overheating or uneven temperature distribution, and provides a channel for the flow and mixing of the liquid within the chamber, promoting uniform heat diffusion in the liquid.

[0126] The circumferential coiling arrangement of the heat exchange pipe 8 extends the heat exchange area along the height or circumference of the chamber, increasing the effective heat exchange area and improving heat exchange efficiency. The number of coils and the pitch are designed based on the chamber volume, target temperature range, and flow rate of the heat exchange medium to achieve a balance between rapid heating and precise temperature control. The width of the heat exchange gap is determined based on the pipe diameter of the heat exchange pipe 8, the chamber size, and liquid circulation requirements, ensuring both a compact structure and sufficient liquid flow space. The two ends of the heat exchange pipe 8 are connected to the heat exchange medium supply system and the return system, respectively, forming a closed loop. The heat exchange medium flows continuously within the pipe, carrying heat into or out of the chamber, and dynamically balancing the temperature is achieved in conjunction with the control commands of the control device 18.

[0127] Preferably, the outer wall of the heat exchange pipe 8 is provided with heat dissipation fins 81. These fins 81 are thin, sheet-like or needle-like protrusions extending outward along the outer wall of the heat exchange pipe 8, forming an integral part or tightly connected to the outer wall. The heat dissipation fins 81 increase the contact area between the heat exchange pipe 8 and the external liquid, enhancing the efficiency of heat transfer from the pipe wall to the liquid and improving the response speed of heating or cooling. The heat dissipation fins 81 are arrayed along the circumference and axial direction of the heat exchange pipe 8, forming a three-dimensionally extended heat exchange surface, allowing the heat exchange pipe 8 to obtain a larger effective heat exchange area within a limited space. This configuration makes temperature control more sensitive and precise, shortens the time required to reach the preset temperature, and improves measurement preparation efficiency and environmental stability.

[0128] In one specific embodiment, the inner wall of the through hole 152 is provided with a stepped surface 153. This stepped surface 153 is a ring-shaped support structure continuously distributed circumferentially along the inner wall of the through hole 152, located in the middle or lower region of the inner wall of the through hole 152, dividing the inner cavity of the through hole 152 into an upper mounting section and a lower transition section. The upper surface of the stepped surface 153 is a horizontal support surface, perpendicular to the axial direction of the through hole 152, used to support the bottom surface of the first water-proof component 20 or the second water-proof component 21. The lower surface of the stepped surface 153 smoothly transitions to the lower inner wall of the through hole 152, forming a conical or arc-shaped flow-guiding structure to reduce resistance to liquid flow.

[0129] The first waterproof component 20 or the second waterproof component 21 is supported from top to bottom on the stepped surface 153. This support achieves axial positioning and vertical support for the waterproof components. The first waterproof component 20 is an integral sealing plate, whose outer contour is adapted to the inner wall of the mounting section above the through hole 152. The bottom surface of the first waterproof component 20 is a flat annular contact surface, forming a surface contact with the upper surface of the stepped surface 153, transferring the weight of the sealing plate and the pressure of the liquid above to the partition 15 structure. The second waterproof component 21 is a modular structure, whose outer contour is also adapted to the inner wall of the mounting section above the through hole 152. The bottom surface of the modular second waterproof component 21 forms a complete annular contact surface, forming a surface contact with the upper surface of the stepped surface 153, achieving stable support for the modular structure.

[0130] The stepped surface 153 allows for precise and controllable installation of the first and second water-proof components 20 and 21, preventing downward displacement or tilting of the components under liquid pressure. The larger contact area of ​​the supporting mating parts disperses pressure concentration, improving sealing reliability and structural durability. Simultaneously, the stepped surface 153 divides the through hole 152 into upper and lower sections: the upper section is used for sealing with the water-proof components, while the lower section allows for liquid flow or plant passage. This clear functional zoning facilitates control over processing and assembly precision. This design enables rapid installation and reliable fixation of the water-proof components, providing structural protection for sealing or connecting the photosynthetic chamber 6 and the oxygen-secreting chamber 9.

[0131] Preferably, a sealing gasket 22 is provided between the first water-proof component 20 and the second water-proof component 21 and the through hole 152. The sealing gasket 22 is an annular sealing element made of elastic material, filling the annular gap between the outer peripheral surface of the water-proof component and the inner wall surface of the through hole 152. The cross-sectional shape of the sealing gasket 22 is circular, rectangular, or lip-shaped, selected according to the sealing pressure and installation space requirements. During the process of the water-proof component being supported from top to bottom on the stepped surface 153, the sealing gasket 22 is subjected to axial compression, resulting in radial elastic deformation, tightly fitting the outer peripheral surface of the water-proof component and the inner wall surface of the through hole 152, forming a reliable static seal. This sealing structure prevents liquid leakage between the photosynthetic chamber 6 and the oxygen-secreting chamber 9 or between the through hole 211 and the root-leaf connection, ensuring the independence of the chambers and the accuracy of the measurements in each working mode. The replaceable design of the sealing gasket 22 facilitates maintenance and adaptability to different sealing requirements, improving the versatility and service life of the equipment.

[0132] In one specific embodiment, the marine plant photosynthetic capacity analysis device further includes a magnetic layer 151 and a fixing element, which together form a magnetic fixing system for the plant leaf, enabling stable positioning and uniform light exposure of the leaf during the measurement process.

[0133] A magnetic layer 151 is laid on the top surface of the partition 15, as well as the top surfaces of the first water-proof component 20 and the second water-proof component 21, forming a continuous and complete magnetic support plane. The magnetic layer 151 is a functional layer with permanent magnet or electromagnetic properties, and its surface magnetic field strength is designed according to the fixing requirements and the characteristics of the plant leaves. The magnetic layer 151 covers the placement area of ​​the plant leaves. Regardless of whether either the first water-proof component 20 or the second water-proof component 21 is connected to the through hole 152, the magnetic layer 151 can form a usable magnetic fixing base on the top surface of the partition 15, ensuring the continuity of the leaf fixing function under different working modes.

[0134] The fastener includes a magnetic attraction part 25, which is a magnetic element or ferromagnetic element with a polarity opposite to that of the magnetic attraction layer 151, and can generate a magnetic force that attracts each other with the magnetic attraction layer 151. The fastener fixes the plant's leaves to the top surface of the partition 15 by magnetic attraction through the magnetic attraction of the magnetic attraction part 25 and the magnetic attraction layer 151.

[0135] The magnetic attraction between the magnetic layer 151 and the fixing element enables non-destructive blade fixation. Magnetic fixation avoids physical damage to the blade tissue caused by mechanical clamping, preserving the integrity of the blade's physiological activity and photosynthetic function. Simultaneously, the magnetic attraction allows the fixing element to quickly adhere and move at any position on the surface of the magnetic layer 151, facilitating flexible adjustment of the fixing point according to the blade's size and shape. This ensures the blade lies flat and adheres to the top surface of the partition 15, reducing leaf wrinkles and overlap, and guaranteeing uniform light exposure and representative measurements. The reversibility of the magnetic fixation makes blade installation and removal convenient and efficient, improving the efficiency and repeatability of the measurement operation.

[0136] In one specific embodiment, the fastener includes a cylindrical clamp 23 and a U-shaped clamp 24, both of which are magnetically engaged with the magnetic layer 151 to adapt to different shapes of marine plant leaves, thereby achieving a targeted fastening method.

[0137] The cylindrical clamp 23 is a cylindrical or prismatic block structure with a magnetic suction part 25 located on its bottom surface. This magnetic suction part 25 is a magnetic or ferromagnetic element with a polarity opposite to that of the magnetic suction layer 151, capable of generating a magnetic force that attracts it. The cylindrical clamp 23 is used to press and fix the sheet-like blades. In use, the sheet-like blades are laid flat on the surface of the magnetic suction layer 151, and the cylindrical clamp 23 is placed on top of the blades. The magnetic suction part 25 on the bottom surface magnetically engages with the magnetic suction layer 151, generating a magnetic force that presses the blades firmly against the top surface of the partition 15. The bottom surface of the cylindrical clamp 23 is a flat pressing surface, forming a surface contact with the blades, dispersing the pressing force and avoiding localized damage. The number and arrangement of the cylindrical clamps 23 can be flexibly adjusted according to the area and shape of the blades. Multiple cylindrical clamps 23 used in conjunction can achieve comprehensive fixation of large-area blades.

[0138] The U-shaped clamp 24 is a groove-shaped structure with a U-shaped cavity opening downwards. This U-shaped cavity is formed by a top wall and two side walls, with the opening facing downwards towards the magnetic layer 151. Magnetic suction parts 25 of the U-shaped clamp 24 are located at the bottom ends of the two side walls of the opening, forming a double-sided magnetic suction structure. The U-shaped clamp 24 is used to clamp and fix thin strip blades. In use, the thin strip blade is inserted into the U-shaped cavity through the opening, and the blade is accommodated within the U-shaped cavity. The magnetic suction parts 25 at the bottom ends of the two side walls simultaneously engage with the magnetic layer 151, generating a double-sided magnetic force that attracts and fixes the entire U-shaped clamp 24 to the top surface of the partition 15. At the same time, the two side walls of the U-shaped cavity form a clamping constraint from both sides of the blade, restricting lateral movement and rotation of the blade. This clamping method allows the thin strip blade to be stably fixed while maintaining its natural extension, avoiding bending or overlapping of the strip blade that might occur with crimping methods.

[0139] The use of either the cylindrical clamp 23 or the U-shaped clamp 24, or their combination, allows the equipment to adapt to the diverse leaf morphologies of marine plants, from broad thallus to slender needle-like leaves, achieving reliable fixation and ensuring that various marine plants are stable in position and receive uniform light during the measurement process, thereby improving the accuracy and applicability of photosynthetic capacity measurement.

[0140] The specific procedures for each measurement using the marine plant photosynthetic capacity analysis equipment described in this application are explained below.

[0141] For measuring the respiration of marine plants: First, level the equipment by observing the bubble level 16 on the support plate 17 to ensure it is horizontal. Open the top cover 1, keeping the partition 15 closed, and place the first water-proof component 20 and the sealing gasket 22 into the through hole 152 in the middle of the partition 15 to completely isolate the photosynthetic chamber 6 from the oxygen-secreting chamber 9. Then, lay the seaweed flat and fix it on the partition 15, and select either the cylindrical clamp 23 or the U-shaped clamp 24 according to the leaf morphology to cooperate with the magnetic layer 151 for fixation. Rotate the top cover 1 and the photosynthetic chamber 6 to seal, forming a sealed space for the photosynthetic chamber 6. Keep the outlet 30 of the photosynthetic chamber 6 closed, open the air outlet 29 and the water inlet 28 of the photosynthetic chamber 6, and add seawater into the photosynthetic chamber 6. After filling, close all valves. Temperature sensor 13, pH sensor 11, light intensity sensor 14, dissolved oxygen sensor 10, and salinity sensor 12 of photosynthesis chamber 6 are immersed in seawater, and the corresponding environmental parameters are monitored in real time by control device 18. According to the measurement requirements, the temperature is adjusted by regulating the heating device through control device 18, and salinity and pH adjustment solutions are injected through inlet 27 to adjust the salinity and pH, restoring each environmental parameter to the preset range. During the measurement process, the impeller 7 can be intermittently or continuously operated to accelerate the uniform mixing of seawater, ensuring that the sensor readings represent the true state of the overall liquid. A light shield 26 is fitted over the outside of photosynthesis chamber 6 and oxygen secretion chamber 9, with the lower edge of the light shield 26 inserted into the light-proof groove 172 to form a snap-fit, achieving complete light blocking. Opening the baffle 262 at the observation hole 261 on the light shield 26 allows observation of the seaweed and equipment. After a preset dark adaptation time, the dissolved oxygen sensor 10 detects the rate of change in dissolved oxygen and calculates the respiration rate. After the measurement, the leaf area was calculated by taking pictures using the image acquisition device 5, the area correction of the fixing parts was removed, the seaweed was taken out and weighed, and the respiration rate of seaweed per unit area or per unit weight was obtained by calculating the difference in dissolved oxygen content over a fixed time period.

[0142] For determining the photosynthetic curve of marine plant leaves: The top cover 1 is opened, with the partition 15 kept closed. The first water-proof component 20 and the sealing gasket 22 are placed in the through hole 152 in the middle of the partition 15, achieving complete isolation between the photosynthetic chamber 6 and the oxygen-secreting chamber 9. The seaweed is then fixed onto the partition 15, and environmental parameters are adjusted. The light intensity received by the plant leaves on the partition 15 is measured using a light intensity sensor 14, ensuring the sensor is not obstructed. The intensity of the light source 3 is set using the control device 18, gradually increasing according to a preset light intensity gradient. Placing the light shield 26 achieves zero light intensity. After stabilizing for a preset time under different light gradients, a fixed time interval is set, and the dissolved oxygen content in the seawater is measured at the start and end times. The difference in dissolved oxygen content within the fixed time interval is calculated to obtain the photosynthetic oxygen release rate under each light intensity. The photosynthetic response curve per unit area or unit weight of seaweed is then plotted, obtaining key parameters such as the light compensation point, light saturation point, and maximum net photosynthetic rate.

[0143] For measuring the oxygen secretion of marine plant roots: The second water-proof component 21 is separated, and the root-leaf connection of the plant is fixed to the central through-hole 211 and then joined together. Soft paraffin wax is inserted into the gaps for sealing. Simultaneously, the second water-proof component 21 and the sealing gasket 22 are placed in the central through-hole 152 of the partition plate 15, so that the plant's leaves are located in the photosynthesis chamber 6 and the roots in the oxygen secretion chamber 9. The air outlet 29 and water inlet 28 of the photosynthesis chamber 6 and oxygen secretion chamber 9 are opened, and seawater is added to both chambers. Different light intensities, salinity, temperature, and pH are set using the control device 18 according to the measurement requirements to bring the environmental parameters of both chambers to the preset range. The increase in dissolved oxygen in the seawater at fixed time intervals is detected by the dissolved oxygen sensor 10 in the oxygen secretion chamber 9, and the root oxygen secretion rate per unit area or unit weight of seagrass is calculated. Since the oxygen content secreted by roots is much lower than that secreted by leaves, sodium sulfite solution can be injected into the oxygen chamber 9 through the inlet 27 controlled by the control device 18. Sodium sulfite, as a chemical oxygen remover, reacts with the dissolved oxygen in the liquid in the oxygen chamber 9, consuming and removing the dissolved oxygen, and reducing the dissolved oxygen concentration in the oxygen chamber 9 before measurement begins. This makes the concentration change caused by root oxygen secretion more significant, thereby improving the detection sensitivity and data resolution of the dissolved oxygen sensor 10.

[0144] For determining the carbonate utilization capacity and pathways of marine plants: Open the top cover 1, keep the partition 15 closed, and place the first water-proof component 20 and the sealing gasket 22 in the through hole 152 in the middle of the partition 15 to completely isolate the photosynthetic chamber 6 from the oxygen-secreting chamber 9. Place the weighed seaweed leaves in the photosynthetic chamber 6, open the air outlet 29 and water inlet 28 of the photosynthetic chamber 6, add seawater into the photosynthetic chamber 6, and adjust the appropriate temperature and salinity conditions. The light intensity was set to saturated light intensity. The initial seawater pH was generally around 8.1. Seagrass was cultivated under saturated light conditions for a set time. As the seagrass leaves carried out photosynthesis under strong light, they continuously consumed carbon dioxide and bicarbonate ions in the seawater and released hydroxide ions, causing the pH of the seawater in photosynthesis chamber 6 to gradually increase until it tended to stabilize. The pH change of the seawater was monitored by acid-base sensor 11, and the final stable pH value was recorded. If the pH value was lower than 9.0, it meant that the seagrass leaves could only use free carbon dioxide in the seawater as a carbon source. If it was higher than 9.0, it meant that it could effectively utilize bicarbonate ions in the seawater. The pH change during the entire measurement process can be used to plot the pH drift curve.

[0145] Once it is determined that seagrass leaves can utilize bicarbonate from seawater, the utilization pathway of bicarbonate in seagrass leaves can be determined by adding inhibitors to eliminate possibilities. The first method verifies that extracellular carbonic anhydrase is the dominant pathway contributing to bicarbonate utilization: Seagrass was placed in photosynthesis chamber 6 as described above, and acetazolamide inhibitor was added through inlet 27 of photosynthesis chamber 6. After photosynthesis chamber 6 was thoroughly mixed with seawater, data was observed. Compared with the inhibitor-treated group, the net photosynthetic rate of the blank control group decreased by 30%-70% after inhibition, and the leaf surface pH increased, confirming that extracellular carbonic anhydrase is the key pathway. The contribution ratio of this pathway was calculated by comparing the differences. The second method verifies that the cotransport of hydrogen ions and bicarbonate is the dominant pathway contributing to bicarbonate utilization: Following the method described above, seaweed was placed in photosynthesis chamber 6, and alkaline buffer triaminomethane hydrochloride was added through inlet 27 of photosynthesis chamber 6. After photosynthesis chamber 6 was thoroughly mixed with seawater, data were observed. Compared with the inhibitor-treated group, the net photosynthetic rate of the blank control group decreased by 30%-70% after inhibition. This indicates that triaminomethane hydrochloride neutralized the acidic zone formed by hydrogen ion efflux from the leaf cell diffusion boundary layer, confirming that the cotransport of hydrogen ions and bicarbonate is a key pathway. The contribution ratio of this pathway was calculated by comparing the differences.

[0146] The following is a detailed description using specific examples.

[0147] Example 1: Measurement of respiration in the thallus of Ulva perforatum:

[0148] Photosynthetic conditions: Photosynthetic chamber 6, volume 100 mL, temperature 25℃, salinity 30‰, pH 8.13.

[0149] Marine plant characteristics: Ulva perforatum, the measured part was the thallus, the leaf area on one side was 2.28 cm², and the dry weight of the thallus was 0.0049 g.

[0150] The measurement time was 15 minutes, and the results are as follows:

[0151] Initial dissolved oxygen content: 194.25 μmol / L (=6.2160 mg / L).

[0152] Termination dissolved oxygen concentration: 191.42 μmol / L (=6.1254 mg / L).

[0153] Dissolved oxygen difference: -2.83 μmol / L.

[0154] Respiration calculation:

[0155] Weight unit: -2.83 × 0.1 ÷ (15 × 0.0049) = -3.85 μmol / (min·g dry weight)

[0156] Area unit: -2.83×0.1×1000÷(15×2.28)=-8.27nmol / (min·cm²).

[0157] Example 2: Determining the photosynthetic curve of Tylian grass:

[0158] Photosynthetic conditions: Photosynthetic chamber 6, volume 100 mL, temperature 27℃, salinity 32‰, pH 8.15.

[0159] Marine plant characteristics: Tylosus tylosus, measured from leaves, dry weight 0.0284g, leaf area 7.47cm².

[0160] The measurement time was 10 minutes, and the specific photosynthetic curve data are shown in Table 1.

[0161] Table 1. Data on photosynthetic curves of Tyrian grass.

[0162]

[0163] A right-angled hyperbola correction model was used to fit the light response curve to the weight unit data, resulting in the photosynthesis curve, as shown below. Figure 26 As shown. The specific parameters for fitting the photosynthetic curve are as follows: α=94.04, R d =-525.80 nmolO2 / (min·gDW), P max =840.57 nmol O2 / (min·gDW), I sat =155.91μmol / (m 2 ·s), I c =8.20 μmol / (m 2 ·s), R 2 =0.99.

[0164] A right-angled hyperbola correction model was used to fit the light response curve to the area unit data, resulting in the photosynthesis curve, as shown below. Figure 27 As shown. The specific parameters for fitting the photosynthetic curve are as follows:

[0165] α=0.36, R d =2.00 nmol O2 / (min·cm) 2 ), P max =3.20 nmol O2 / (min·cm) 2 ), I sat =155.91μmol / (m 2 ·s), I c =8.20 μmol / (m 2 ·s), R 2 =0.99.

[0166] Abbreviation: α is the initial slope of the light response curve, R d P represents the rate of dark respiration. max For the maximum net photosynthetic rate, I sat I is the light saturation point. c R is the light compensation point. 2 The fit is denoted as .

[0167] Example 3: Measuring the root oxygen secretion rate of *Hylocereus undatus*:

[0168] Photosynthetic conditions: Oxygen-secreting chamber 9, volume 100 mL, temperature 26℃, salinity 33‰, pH 8.12.

[0169] Marine plant characteristics: Roundleaf filamentous grass, stem and root fragments, the measured part was the root fragment, the fresh weight of the root was 0.7458gFW, and the dry weight of the root was 0.1706gDW.

[0170] The measurement lasted for 30 minutes, and the specific data recorded are shown in Table 2.

[0171] Table 2. Data on oxygen secretion rate of *Hylocereus undatus* root system.

[0172]

[0173] Example 4: Determining the carbonate utilization capacity of needlegrass:

[0174] Photosynthetic conditions: Photosynthetic chamber 6, volume 100 mL, temperature 25℃, salinity 32‰, PAR 150 μmol / (m³) 2 ·s), initial pH 8.13, initial dissolved oxygen content 126.75 μmol / L.

[0175] Marine plant characteristics: needlegrass, measured part was needle-like leaves, fresh weight 0.15g.

[0176] The measurement period was 24 hours, and the specific pH data are recorded in Table 3 and... Figure 28 As shown.

[0177] Table 3. pH data for determining the carbonate utilization capacity of needlegrass.

[0178]

[0179] The data in the table shows that the pH compensation point is greater than 9.0, indicating that needlegrass can utilize bicarbonate as a carbon source.

[0180] Example 5: Determining the proportion of bicarbonate applied by *Botrytis cinerea* via the carbonic anhydrase acidification pathway:

[0181] Photosynthetic conditions: Photosynthetic chamber 6, volume 100 mL, temperature 25℃, salinity 32‰, pH 8.20, light intensity 75 μmol / (m²). 2 ·s).

[0182] Marine plant characteristics: *Botrytis cinerea*, measured on an erect branch, wet weight 0.53 g.

[0183] The measurement lasted for 10 minutes, and the specific results are as follows:

[0184] Control group: initial dissolved oxygen content 126.47 μmol / L (=4.0470 mg / L), final dissolved oxygen content 129.32 μmol / L (=4.1382 mg / L), dissolved oxygen difference 2.85 μmol / L.

[0185] Net photosynthetic intensity (as a control): 2.85 × 0.1 × 1000 ÷ 10 ÷ 0.53 = 53.77 nmol / (min·gFW).

[0186] In the acetazolamide inhibitor group, 5 mL of 20 mmol / L acetazolamide was added, resulting in a final acetazolamide concentration of 100 μmol / L. The initial dissolved oxygen content was 137.58 μmol / L (=4.4026 mg / L), the final dissolved oxygen content was 139.02 μmol / L (=4.4486 mg / L), and the dissolved oxygen difference was 1.44 μmol / L.

[0187] Net photosynthetic intensity after treatment with acetazolamide inhibitor: 1.44 × 0.1 × 1000 ÷ 10 ÷ 0.53 = 27.17 nmol / (min·gFW).

[0188] The inhibition rate of acetazolamide was (53.77-27.17)÷53.77×100%=49.47%, meaning that the proportion of bicarbonate applied by *Gracilaria longifolia* through the carbonic anhydrase acidification pathway was 49.47%.

[0189] By applying the technical solution provided in this invention, live marine plants can be fixed in the device. This allows for the measurement of leaf and root respiration, leaf photosynthesis, the plant's ability to utilize seawater carbonate and the pathways of carbonate utilization, and root oxygen secretion under controlled conditions, while ensuring a stable seawater environment and simultaneously controlling light, temperature, and seawater pH. The device possesses a stable and reliable environmental regulation function for plant photosynthesis, solving the problem of unstable external environments encountered when measuring the photosynthetic capacity of marine plants' leaves and roots, thereby improving the accuracy of measuring multiple indicators related to seagrass photosynthetic capacity.

[0190] The various embodiments in this specification are described in a progressive manner, with each embodiment focusing on the differences from other embodiments. The same or similar parts between the various embodiments can be referred to each other.

[0191] The above description of the disclosed embodiments enables those skilled in the art to make or use the invention. Various modifications to these embodiments will be readily apparent to those skilled in the art, and the general principles defined herein may be implemented in other embodiments without departing from the spirit or scope of the invention. Therefore, the invention is not to be limited to the embodiments shown herein, but is to be accorded the widest scope consistent with the principles and novel features disclosed herein.

Claims

1. A device for analyzing the photosynthetic capacity of marine plants, characterized in that, include: The photosynthesis chamber (6) and the oxygen secretion chamber (9) are located below the photosynthesis chamber (6). The bottom wall of the photosynthesis chamber (6) and the top wall of the oxygen secretion chamber (9) share the same partition (15). A through hole (152) is provided on the partition (15). The first water-proof component (20) and the second water-proof component (21) are provided with a through hole (211) for the plant root and leaf connection part to pass through. The first waterproof component (20) and the second waterproof component (21) are optionally sealed and connected within the through hole (152); When the first water-proof component (20) is connected to the through hole (152), the photosynthesis chamber (6) and the oxygen-secreting chamber (9) are isolated from each other; When the second water-proof component (21) is connected to the through hole (152), the root-leaf connection of the plant is sealed through the through hole (211), so that the leaf part of the plant is located in the photosynthesis chamber (6) and the root part of the plant is located in the oxygen secretion chamber (9). The photosynthesis chamber (6) is equipped with a light source (3). Both the photosynthesis chamber (6) and the oxygen secretion chamber (9) are equipped with a heating device, a water inlet (28) and a liquid inlet (27). The water inlet (28) is connected to the seawater supply pipeline, and the liquid inlet (27) is selectively connected to either the salinity adjustment liquid supply pipeline or the pH adjustment liquid supply pipeline. The marine plant photosynthetic capacity analysis device also includes: A detection device for real-time detection of environmental parameters within the photosynthesis chamber (6) and / or the oxygen secretion chamber (9); The control device (18) is electrically connected to the detection device, the light source (3), the heating device, the water inlet (28), and the liquid inlet (27); The control device (18) is configured to adjust the light intensity of the light source (3), the heating power of the heating device, the water inlet (28) and / or the liquid inlet (27) when the environmental parameters detected by the detection device deviate from the preset range before the plant photosynthetic capacity is measured, so that the environmental parameters are restored to the preset range.

2. The marine plant photosynthetic capacity analysis device according to claim 1, characterized in that, The second waterproof component (21) includes at least two sector-shaped sub-plates, the radial sides of each sector-shaped sub-plate are joined together, and the inner arcs of each sector-shaped sub-plate are enclosed to form the through hole (211). The second water-proof component (21) is embedded in the through hole (152), and the outer arc of the fan-shaped sub-plate is sealed and fitted with the inner peripheral wall of the through hole (152).

3. The marine plant photosynthetic capacity analysis device according to claim 1, characterized in that, A stirring impeller (7) is provided in the photosynthesis chamber (6) and / or the oxygen secretion chamber (9). The stirring impeller (7) is electrically connected to the control device (18), which is configured to control the stirring impeller (7) to operate intermittently or continuously during the measurement process.

4. The marine plant photosynthetic capacity analysis device according to claim 1, characterized in that, The detection device is provided in both the photosynthesis chamber (6) and the oxygen secretion chamber (9). The detection device includes at least one set of sensor components, including a temperature sensor (13), an acid-base sensor (11), a light intensity sensor (14), a dissolved oxygen sensor (10), and a salinity sensor (12). The sensor assembly is installed on the inner wall of the corresponding chamber; The control device (18) is located below the oxygen-secreting chamber (9), and the top wall of the control device (18) and the bottom wall of the oxygen-secreting chamber (9) are formed by the same support plate (17).

5. The marine plant photosynthetic capacity analysis device according to claim 4, characterized in that, The control device (18) is configured to perform at least one of the following measurement modules: The respiration measurement module controls the light source (3) to turn off and block the photosynthesis chamber (6). After a preset dark adaptation time, the dissolved oxygen sensor (10) detects the rate of change of dissolved oxygen and calculates the respiration rate. The photosynthesis curve measurement module controls the light source (3) to be adjusted step by step according to the preset light intensity gradient. After stabilizing for a preset time under each light intensity, the dissolved oxygen sensor (10) detects the rate of change of dissolved oxygen and plots the photosynthesis response curve. The root oxygen secretion measurement module controls the second water-proof component (21) to be installed in the through hole (152), so that the plant roots are located in the oxygen secretion chamber (9), and the root oxygen secretion rate is detected by the dissolved oxygen sensor (10) in the oxygen secretion chamber (9). The carbonate utilization capacity determination module controls the light source (3) to maintain saturated light intensity, monitors the pH drift of the culture medium through the acid-base sensor (11), and determines the carbonate utilization capacity based on the pH compensation point. Carbonate utilization pathway assay module: control the injection of inhibitors or buffer solutions into the inlet (27), compare the changes in photosynthetic rate before and after inhibition, and determine the contribution ratio of the extracellular carbonic anhydrase pathway or the proton pump pathway.

6. The marine plant photosynthetic capacity analysis device according to claim 5, characterized in that, Also includes: A light shield (26) is detachably fitted onto the outside of the photosynthesis chamber (6) and the oxygen secretion chamber (9); The annular boss is formed by the outer edge of the bottom wall of the oxygen-secreting chamber (9) extending radially outward, and the lower end face of the light shield (26) abuts against the top surface of the annular boss.

7. The marine plant photosynthetic capacity analysis device according to claim 1, characterized in that, The heating device is a heat exchange pipe (8), which is arranged circumferentially around the inner wall of the photosynthesis chamber (6) or the oxygen secretion chamber (9), and maintains a heat exchange gap with the inner wall surface of the corresponding chamber.

8. The marine plant photosynthetic capacity analysis device according to claim 1, characterized in that, The inner wall of the through hole (152) is provided with a stepped surface (153), and the first water-proof component (20) or the second water-proof component (21) is supported on the stepped surface (153) from top to bottom.

9. The marine plant photosynthetic capacity analysis device according to claim 1, characterized in that, Also includes: A magnetic layer (151) is laid on the top surface of the partition (15), and on the top surfaces of the first waterproof component (20) and the second waterproof component (21); The fastener includes a magnetic part (25) that magnetically engages with the magnetic layer (151) to fix the leaf part of the plant to the top surface of the partition (15).

10. The marine plant photosynthetic capacity analysis device according to claim 9, characterized in that, The fastener includes a cylindrical clamp (23) and a U-shaped clamp (24), both of which are magnetically attracted to the magnetic layer (151). The magnetic suction part (25) of the cylindrical clamp (23) is provided on its bottom end face for pressing and fixing the sheet-like blade; The U-shaped clamp (24) has a U-shaped cavity with the opening facing downwards. Its magnetic suction part (25) is disposed at the bottom of the two side walls of the opening, which is used to clamp and fix the thin strip blade. After the thin strip blade enters the U-shaped cavity through the opening, the magnetic suction part (25) of the U-shaped clamp (24) magnetically engages with the magnetic suction layer (151).

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