Use of a nanosuspension composition for controlling allium leaf miner
By combining cyromazine and lufenuron nano-suspension, the issues of rapid and sustained efficacy in the control of leek maggots were resolved, pesticide residues were reduced, control effects were improved, and the amount used was reduced.
Patent Information
- Authority / Receiving Office
- CN · China
- Patent Type
- Applications(China)
- Current Assignee / Owner
- SHAANXI THOMPSON BIOTECHNOLOGY CO LTD
- Filing Date
- 2024-12-05
- Publication Date
- 2026-06-12
AI Technical Summary
Existing pesticide products for controlling leek maggots have poor fast-acting properties, are difficult to penetrate deep into the soil, and are used in large quantities, resulting in excessive pesticide residues and serious environmental pollution.
A nano-suspension is prepared by mixing cyromazine and lufenuron in a certain proportion. The preparation method includes high-speed shearing, crushing and fine grinding to form a nano-suspension composition with a particle size controlled at 50-300 nanometers, which is used to control leek maggots.
It achieves rapid and sustained control of leek maggots, reduces pesticide residues, shortens the safety interval, and improves control effectiveness.
Smart Images
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Abstract
Description
Technical Field
[0001] This invention belongs to the field of pesticide technology, specifically relating to the application of a nano-suspension composition containing cyromazine for the control of leek maggots. Background Technology
[0002] Cyromazine is a triazine insect growth regulator with specific activity against dipteran larvae. It can induce morphological abnormalities in dipteran larvae and pupae, resulting in incomplete or inhibited adult emergence. It is used to control flies in animal stables and to control agricultural pests such as the American serpentine leafminer on cucumbers, eggplants, green beans, leafy vegetables, and flowers.
[0003] Lufenuron is the latest generation of urea-substituted insecticides. It kills pests by acting on insect larvae and inhibiting molting, exhibiting excellent efficacy against leaf-eating caterpillars, especially on fruit trees. It also has a unique killing mechanism against thrips, rust mites, and whiteflies, making it suitable for controlling pests resistant to synthetic pyrethroids and organophosphate pesticides. The pesticide has a long residual effect, reducing the number of applications required. It is safe for crops such as corn, vegetables, citrus, cotton, potatoes, grapes, and soybeans, and is suitable for integrated pest management. It is primarily used to control lepidopteran larvae in cotton, corn, vegetables, and fruit trees; it can also be used as a sanitary pesticide; and it can be used to control piercing-sucking pests.
[0004] Leek maggots typically have four generations per year, occurring in early May, mid-June, early August, and late September. They overwinter as pupae, with large-scale emergence of adults and larvae from late July to early August. Larvae swarm and damage the underground rhizomes of leeks. Adults prefer damp, shady conditions, are adept at flying and walking, and are very active, often inhabiting crevices in the soil around leek roots. Mature larvae or pupae overwinter in the soil 3-4 cm deep within the leek bulbs and around the roots. Adults are photophobic, prefer moist conditions, and are attracted to dryness, showing a strong attraction to the odors emitted by onions and garlic. Eggs are mostly laid in the soil around the leek rhizomes. Larvae damage the underground leaf sheaths, tender stems, and buds of leeks, biting off tender stems and boring into the bulbs. In open-field leek fields, leek maggot larvae are distributed in the soil 2-3 cm below the ground, with a maximum depth of 5-6 cm.
[0005] Due to the limited life cycle of leek maggots, existing control products are scarce. With national restrictions on highly toxic pesticides, even fewer effective insecticides exist, and those that are available are primarily granular. Granules, however, have poor speed of action and struggle to penetrate deep into the soil, rendering them ineffective against maggot pupae. To achieve pest control, farmers often increase pesticide dosage and frequency, leading to excessive pesticide residues in leeks and increased environmental pollution. To address this technical challenge, the applicant has developed a nano-suspension formulation by mixing cyromazine and lufenuron in a specific ratio. This formulation effectively solves the problem, offering both rapid and sustained efficacy. Furthermore, this invention reduces pesticide residue levels and shortens the safety interval, making it safer for consumers. Therefore, the combination of cyromazine and lufenuron is worthy of widespread application in agricultural production. Summary of the Invention
[0006] The purpose of this invention is to provide a nano-suspension composition containing cyromazine for the control of leek maggots. It can effectively control leek maggots, has both rapid and long-lasting effects, and has a good control effect on resistant pests, reducing residue and shortening the safety interval.
[0007] The technical solution of this invention is:
[0008] An application of a nano-suspension composition for controlling leek maggots, characterized in that: the nano-suspension composition comprises cyromazine, lufenuron, and adjuvants, wherein the weight ratio of cyromazine to lufenuron is 9:1 to 1:1, optionally the weight ratio of cyromazine to lufenuron is 9:1 to 3:1, and optionally the weight ratio is 6:1.
[0009] Furthermore, the effective active ingredient content of cyromazine and lufenuron is 140–560 g / L, and optionally 350 g / L.
[0010] Further, the nano-suspension composition, based on 1000 parts by weight, comprises the following raw materials in the following proportions: 120-480 parts by weight of cyromazine, 20-100 parts by weight of lufenuron, 5-100 parts by weight of dispersant, 3-60 parts by weight of wetting agent, 10-80 parts by weight of antifreeze, 0.5-10 parts by weight of preservative, 0.5-10 parts by weight of defoamer, 1-20 parts by weight of thickener, 1-10 parts by weight of pH adjuster, and water as the balance.
[0011] Further, the nano-suspension composition comprises, based on 1000 parts by weight, the following raw materials: 300 parts by weight of cyromazine, 50 parts by weight of lufenuron, 25-50 parts by weight of dispersant, 10-30 parts by weight of wetting agent, 40-50 parts by weight of antifreeze agent, 1-4 parts by weight of preservative, 1-3 parts by weight of defoamer, 2-10 parts by weight of thickener, 1-6 parts by weight of pH adjuster, and water as the balance.
[0012] Furthermore, the preparation method of the nano-suspension composition involves first adding water, dispersant, wetting agent, antifreeze, preservative, cyromazine technical, and lufenuron technical into a mixing tank and shearing them at high speed to fully disperse them, forming an initial dispersion. This dispersion is then pumped to a sand mill for pulverization. The feed rate of the sand mill is adjusted to control the fineness at 3-5 μm. This slurry is then finely ground using a pin mill to control the particle size at 50-300 nanometers, confirming that the particle size meets the requirements. Finally, the slurry is transferred to a mixing vessel, where thickener and defoamer are added to adjust the viscosity. Samples are taken for quality testing to obtain the finished product.
[0013] Furthermore, the effective dosage of the nano-suspension composition for controlling leek maggots is 600-1500 g / ha, and optionally 1000-1350 g / ha.
[0014] Furthermore, the nano-suspension composition is used to control leek maggots, with an experimental water consumption of 3000-5000 liters / hectare, and an optional water consumption of 4500 liters / hectare.
[0015] Compared with the prior art, the composition of the present invention has the following beneficial effects: (1) Compared with single agents, the nano-suspension composition has a significant synergistic effect on leek maggots, which improves the control effect; (2) It is low in toxicity and high in efficiency, which reduces the amount of pesticides used, reduces the amount of pesticide residues on crops, and has a short safety interval; (3) It has both fast-acting and long-acting effects. Detailed Implementation
[0016] The present invention will be further described below with reference to embodiments, but the present invention is not limited thereto.
[0017] Application Example 1
[0018] The present invention will be further described below with reference to the embodiments. The components are composed of the following amounts based on 1000 parts by weight, but the present invention is not limited thereto.
[0019] Example 1: 350 g / L cyromazine·lufenuron nano-suspension
[0020] 300 parts of cyromazine, 50 parts of lufenuron, 20 parts of alkyl naphthalene sulfonate, 20 parts of polycarboxylate, 15 parts of fatty alcohol polyoxyethylene ether, 15 parts of alkyl glycoside, 40 parts of ethylene glycol, 1 part of sodium benzoate, 1 part of silicone defoamer, 2 parts of xanthan gum, 5 parts of silica, 3 parts of citric acid, and water to a total of 1000 parts.
[0021] Example 2: 350 g / L cyromazine·lufenuron nano-suspension
[0022] 300 parts of cyromazine, 50 parts of lufenuron, 25 parts of fatty alcohol polyoxyethylene ether phosphate, 10 parts of EO-PO block copolymer, 10 parts of trisiloxane polyoxyethylene ether, 10 parts of alkyl naphthalene sulfonate, 40 parts of propylene glycol, 2 parts of potassium sorbate, 2 parts of C8-10 fatty alcohols, 4 parts of magnesium aluminum silicate, 3 parts of silica, 1 part of citric acid, and water to a total of 1000 parts.
[0023] Example 3: 490 g / L cyromazine·lufenuron nano-suspension
[0024] 420 parts of cyromazine, 70 parts of lufenuron, 30 parts of phenethylphenol polyoxyethylene ether phosphate, 10 parts of alkyl sulfonate, 15 parts of polyarylphenol polyoxyethylene ether, 10 parts of sodium dodecylbenzenesulfonate, 50 parts of glycerol, 1 part of isothiazolinone, 1 part of C10-20 saturated fatty acid compound, 1 part of xanthan gum, 2 parts of silica, 1 part of glacial acetic acid, and water to a total of 1000 parts.
[0025] Example 4: 140 g / L cyromazine·lufenuron nano-suspension
[0026] 120 parts of cyromazine, 20 parts of lufenuron, 15 parts of alkyl naphthalene sulfonate formaldehyde polymer, 10 parts of castor oil polyoxyethylene ether, 40 parts of sorbitol, 1 part of sodium benzoate, 1 part of silicone defoamer, 1 part of polyvinyl alcohol, 3 parts of silica, 2 parts of glacial acetic acid, and water to a total of 1000 parts. Example of application:
[0027] Experiment 1: Indoor Combined Toxicity Determination of Cyromazine, Lufenuron, and Mixed Pairs with Leek Maggots 1. Experimental Objective
[0028] The toxicity of cyromazine and lufenuron, as well as their different mixtures, to leek maggots was determined in the laboratory, and their synergistic effects were evaluated to clarify their compatibility and provide a scientific basis for the research and development of cyromazine and lufenuron mixtures.
[0029] 2 Experimental conditions
[0030] 2.1 Test Target
[0031] Leek maggots are the larvae of the leek maggot. The larvae are collected from leek stubble with obvious signs of damage in the field and taken back to the laboratory for experimental purposes.
[0032] 2.2 Cultivation Conditions
[0033] The culture conditions for the test targets and the targets after the experiment were: temperature (25±1)℃ and relative humidity 60%~80%.
[0034] 3 Experimental Design
[0035] 3.1 Test reagents
[0036] Cyromazine 98% technical grade; Lufenuron 98% technical grade.
[0037] 3.2 Reagent Preparation
[0038] Weigh 0.0306g of 98% technical grade cyromazine, dissolve it in 3mL of LDM, add 0.6mL of Tween 80 emulsifier, stir well, add water to 300mL to prepare a 100mg / L stock solution. Take 80mL of the stock solution and add it to 20mL of water containing 0.1% Tween 80 emulsifier to prepare a 80mg / L test solution. Then dilute it with water containing 0.1% Tween 80 emulsifier at a ratio of 2 to prepare 40, 20, 10, 5 and 2.5mg / L, for a total of 6 concentrations for testing.
[0039] Weigh 0.0204g of 98% lufenuron technical grade, dissolve it in 2mL of LDMF, add 0.4mL of Tween 80 emulsifier and stir well. Add water to 200mL to prepare a 100mg / L stock solution. Take 20mL of the stock solution and add it to 80mL of water containing 0.1% Tween 80 emulsifier to prepare a 20mg / L test solution. Then dilute it with water containing 0.1% Tween 80 emulsifier at a ratio of 2 to prepare 10, 5, 2.5, 1.25 and 0.625mg / L, for a total of 6 concentrations, for testing.
[0040] Based on the ratios of cyromazine to lufenuron (9:1, 6:1, 3:1, 1:1, and 1:3), 36, 34.28, 30, 20, and 5 mL of cyromazine stock solution were measured, respectively. Then, 4, 5.72, 10, 20, and 15 mL of lufenuron stock solution were measured accordingly. The two solutions were mixed, and water with emulsifier was added to bring the total volume to 100 mL. Test solutions of 40, 40, 40, 40, and 20 mg / L were prepared. Each solution was then diluted with water containing 0.1% Tween80 emulsifier at a 2-fold ratio to obtain six different concentrations for testing.
[0041] Add water containing 0.1% Tween 80 emulsifier to a beaker containing 2 mL of LDMF to a final volume of 100 mL as a blank control.
[0042] 4. Test methods
[0043] Following the method outlined in the pesticide indoor bioassay test guidelines NY / T1154.6-2006, the stomach poison contact method was employed. Second-instar larvae of uniform size were selected using a brush and placed into sterilized petri dishes containing filter paper, with 20 larvae per dish, repeated four times. 500 mL of each pesticide solution was added to the larvae using a pipette. The white portion of the leek stem base was then cut into 2 cm segments, immersed in the pesticide solution for 15 seconds, removed, and excess pesticide was absorbed with absorbent paper. Five segments were then placed in each petri dish containing filter paper treated with the same pesticide solution. The dishes were incubated at 25±1℃ in a dark incubator, with timely replenishment of moisture and the addition of leek stems not soaked in pesticide solution.
[0044] 5 Data Investigation and Statistical Analysis
[0045] 5.1 Investigation Time and Method
[0046] After 120 h, check the death condition of the test insects and make records. The judgment criterion for the death of test insects is: gently touch the insect body with the tip of a writing brush, and those that do not move are regarded as dead.
[0047] 5.2 Data Statistical Analysis
[0048] Use DPS data processing software to calculate the test results, and respectively obtain the toxicity regression equations and LC 50 、LC 90 as well as the 95% confidence limits, and record the original data of all repetitions of each treatment.
[0049] Calculate the co-toxicity coefficient (CTC) according to the Sun Yunpei method, and evaluate the synergistic effect of the mixed use of pesticides based on the co-toxicity coefficient (CTC). That is, CTC ≤ 80 is antagonistic effect, 80 < CTC < 120 is additive effect, and CTC ≥ 120 is synergistic effect. The calculation formula for the co-toxicity coefficient is as follows:
[0050] Standard pesticide LC 50
[0051] Actual toxicity index (ATI) = ———————— × 100
[0052] LC of the tested mixture 50
[0053] Theoretical toxicity index (TTI) of the mixture = Toxicity index TI of pesticide A A × Percentage content (%) of pesticide A in the mixture + Toxicity index of pesticide B × Percentage content (%) of pesticide B in the mixture
[0054]
[0055] 5 Experimental Results
[0056] Table 1 Toxicity determination results of the mixture of cyromazine and lufenuron against Bradysia odoriphaga in Chinese chives
[0057]
[0058] It can be seen from Table 1 of the test results that the mixtures of cyromazine and lufenuron at the ratios of 9:1, 6:1, 3:1, and 1:1 showed a synergistic effect against Bradysia odoriphaga in Chinese chives, among which the synergistic effect of 6:1 was the most significant.
[0059] Application Example 3: Field efficacy test of cyromazine·lufenuron nano-suspension against Bradysia odoriphaga in Chinese chives
[0060] 1 Test Purpose
[0061] To verify the efficacy and safety of the 350 g / L cyromazine·lufenuron nano-suspension agent developed by the applicant against leek maggots, to clarify the field dosage and application technology, and to provide a scientific basis for pesticide registration.
[0062] 2. Test Basis
[0063] Refer to Part 67 of GB / T 17980.67-2004, "Guidelines for Field Efficacy Testing of Pesticides (II): Control of Leek Maggots and Root Maggots with Insecticides".
[0064] 3 Experimental Locations
[0065] This experiment was conducted in Guangzhou, Guangdong Province, Shandong Province, and Hunan Province.
[0066] 4. Selection of test subjects, crops and varieties
[0067] Test subject: Leek maggots
[0068] 5. Experimental Design and Arrangement
[0069] 5.1 Dosage and Numbering of Pharmaceuticals
[0070] Table 2 Experimental Design of Test Reagents
[0071]
[0072] 5.2 Application time and frequency
[0073] Apply pesticide once by drenching the roots of chives at the initial stage of chive maggot infestation.
[0074] 5.3 Usage Capacity
[0075] The amount of pesticide solution sprayed per hectare is 4,500 liters.
[0076] 5.4 Survey Time and Frequency
[0077] Five surveys were conducted before application of the medication and at 3, 7, 14, and 21 days after application.
[0078] 5.5 Method for Calculating Drug Efficacy
[0079] The field efficacy calculation is based on the formula in the "Guidelines for Field Efficacy Trials of Pesticides" to calculate the insect population reduction rate and control effect.
[0080]
[0081] 6 Experimental Results
[0082] Table 3. Results of field efficacy trials of cyromazine-lufenuron nano-suspension and control agents for controlling leek maggots.
[0083]
[0084] The experimental agents and control single-agent formulations for controlling leek maggots were shown in Table 3. Table 3 shows that when the effective ingredient dosage of cyromazine·lufenuron nano-suspension was 1050–1312.5 g / ha, the plant survival rate was 56.02%–69.41% three days after application; the control single-agent formulation of 10% lufenuron suspension with an effective ingredient dosage of 375 g / ha showed a plant survival rate of 47.31%–62.72% three days after application; and the control single-agent formulation of 50% cyromazine wettable powder with an effective ingredient dosage of 1500 g / ha showed a plant survival rate of 50.71%–56.41% three days after application.
[0085] When the effective ingredient dosage of cyromazine·lufenuron nano-suspension was 1050–1312.5 g / ha, the plant survival rate was 75.34%–85.70% 7 days after application. For the control agent, 10% lufenuron suspension with an effective ingredient dosage of 375 g / ha, the plant survival rate was 68.17%–78.78% 7 days after application. For 50% cyromazine wettable powder with an effective ingredient dosage of 1500 g / ha, the plant survival rate was 60.13%–72.15% 7 days after application.
[0086] When the effective ingredient dosage of cyromazine·lufenuron nano-suspension was 1050–1312.5 g / ha, the plant survival rate was 80.49%–87.48% 14 days after application. For the control agent, 10% lufenuron suspension with an effective ingredient dosage of 375 g / ha, the plant survival rate was 79.30%–84.26% 14 days after application. For 50% cyromazine wettable powder with an effective ingredient dosage of 1500 g / ha, the plant survival rate was 70.42%–78.69% 14 days after application.
[0087] When the effective ingredient dosage of cyromazine·lufenuron nano-suspension was 1050–1312.5 g / ha, the plant survival rate was 80.43%–86.36% 21 days after application. For the control agent, 10% lufenuron suspension with an effective ingredient dosage of 375 g / ha, the plant survival rate was 79.60%–83.26% 21 days after application. For 50% cyromazine wettable powder with an effective ingredient dosage of 1500 g / ha, the plant survival rate was 77.30%–80.26% 21 days after application.
[0088] When the effective ingredient dosage of cyromazine·lufenuron nano-suspension was 1050–1312.5 g / ha, the control efficacy was 82.83%–87.42% 21 days after application. For the control agent, 10% lufenuron suspension with an effective ingredient dosage of 375 g / ha, the control efficacy was 80.07%–83.79% 21 days after application. For 50% cyromazine wettable powder with an effective ingredient dosage of 1500 g / ha, the control efficacy was 79.27%–82.14% 3 days after application.
[0089] Analysis of variance showed that, among the experimental groups, the effective ingredient dosage of cyromazine·lufenuron nano-suspension at 1050–1312.5 g / ha was significantly different from that of the control single agent 10% lufenuron suspension at 375 g / ha and 50% cyromazine wettable powder at 1500 g / ha. This indicates that the experimental agent cyromazine·lufenuron nano-suspension was significantly superior to the control single agent in controlling leek maggots, demonstrating a synergistic effect.
[0090] During the experiment, no effects of the test agent on chives, such as yellowing spots, loss of green color, whitening, deformity, or growth inhibition, were found.
[0091] The above description, in conjunction with specific preferred embodiments, provides a further detailed explanation of the present invention. It should not be construed that the specific implementation of the present invention is limited to these descriptions. For those skilled in the art, various simple deductions or substitutions can be made without departing from the concept of the present invention, and all such modifications and substitutions should be considered within the scope of protection of the present invention.
Claims
1. An application of a nano-suspension composition for controlling leek maggots, characterized in that: The nano-suspension composition comprises cyromazine, lufenuron, and adjuvants, wherein the weight ratio of cyromazine to lufenuron is 9:1 to 1:
1.
2. The application according to claim 1, characterized in that: The weight ratio of cyromazine to lufenuron is 9:1 to 3:1, and optionally 6:
1.
3. The application according to claim 1 or 2, characterized in that: The effective active ingredient content of cyromazine and lufenuron is 140~560 g / L, and optionally the effective active ingredient content is 350 g / L.
4. The application according to claim 3, characterized in that: The nano-suspension composition comprises, based on 1000 parts by weight, the following raw materials: 120-480 parts by weight of cyromazine, 20-100 parts by weight of lufenuron, 5-100 parts by weight of dispersant, 3-60 parts by weight of wetting agent, 10-80 parts by weight of antifreeze, 0.5-10 parts by weight of preservative, 0.5-10 parts by weight of defoamer, 1-20 parts by weight of thickener, 1-10 parts by weight of pH adjuster, and water as the balance.
5. The application according to claim 4, characterized in that: The nano-suspension composition comprises, by weight, the following raw materials in the following proportions per 1000 parts: 300 parts by weight of cyromazine, 50 parts by weight of lufenuron, 25-50 parts by weight of dispersant, 10-30 parts by weight of wetting agent, 40-50 parts by weight of antifreeze agent, 1-4 parts by weight of preservative, 1-3 parts by weight of defoamer, 2-10 parts by weight of thickener, 1-6 parts by weight of pH adjuster, and water as the balance.
6. The application according to claim 1, characterized in that: The effective dosage of the nano-suspension composition for controlling leek maggots is 600-1500 g / ha, and optionally 1000-1350 g / ha.