Microfluidic microbial enrichment and detection device with filter capture layer

Abstract

The application relates to the technical field of microorganism detection, and discloses a microfluidic microorganism enrichment detection device with a filter membrane capture layer, which comprises an outer box and a sample inlet pipe fixedly installed at the upper end of the outer box, and an enrichment detection assembly is arranged in the outer box; a miniature servo motor is used to drive the filter membrane plate to tilt and rotate, hollow cylinder reverse resistance flow and liquid inlet design are used, the disadvantages of traditional single-center straight-through liquid inlet are abandoned, the detection reagent is uniformly covered and flushes the whole domain of the filter membrane, specific reactions of microorganisms and the reagent on the filter membrane surface are fully carried out, the detection signal response value is effectively improved, the false negative risk of a low-abundance sample is reduced, the detection error is reduced, and the reliability and popularization and application value of the microfluidic microorganism enrichment detection device are remarkably improved.

Description

Technical Field

[0001] This invention relates to the field of microbial detection technology, and in particular to a microfluidic microbial enrichment and detection device with a filter membrane capture layer. Background Technology

[0002] With the rapid development of fields such as food safety supervision, rapid diagnosis of clinical infectious diseases, environmental water monitoring, and public health emergency testing, the core requirements for the detection of pathogenic microorganisms have been put forward: rapid, highly sensitive, portable, low-cost, and on-site.

[0003] In recent years, microfluidic chip technology has become a core research and development direction and mainstream technology route in the field of rapid microbial detection due to its advantages such as low reagent consumption, fast analysis speed, high integration, strong portability, and ability to achieve multi-module integration.

[0004] In microfluidic detection of microorganisms, the abundance of target microorganisms in actual test samples is usually low, and the sample matrix is ​​complex, containing a large number of interfering components such as cell debris, protein impurities, and particulate matter. Direct detection often fails to achieve the required detection sensitivity and is prone to false negative results. Therefore, efficient microbial enrichment pretreatment is the core key to achieving high-sensitivity detection of low-abundance samples, directly determining the detection limit, recovery rate, and accuracy of the detection method.

[0005] Currently, microbial enrichment technologies in microfluidic systems are mainly classified into several categories, including inertial focusing, dielectrophoresis enrichment, immunomagnetic bead enrichment, sonication enrichment, and membrane filtration enrichment. Among them, membrane filtration enrichment, based on the principle of size sieving, achieves efficient separation of target microorganisms from the sample matrix and impurities through the pore size design of the filter membrane. It has advantages such as high enrichment efficiency, no need for additional complex driving equipment, no dependence on biomarkers, simple operation, and low cost. It is currently the microfluidic enrichment technology route that is easiest to realize industrialization and field application, and has become a research hotspot in this field. However, the aforementioned and existing related technologies often have the following drawbacks: the single-center direct-flow inlet layout of existing devices means that the reconstituted test reagent can only form an effective reaction on the surface of the central inlet area of ​​the filter membrane, failing to uniformly wet the enriched area of ​​the filter membrane. This results in the target microorganisms trapped on the filter membrane surface not being able to fully and specifically recognize and react with the test reagent, causing a significant attenuation of the subsequent test signal response value and a significant decrease in detection sensitivity. Furthermore, low-abundance test samples are highly prone to false negative results. Due to the large differences in the degree of reaction in different areas of the filter membrane, the relative standard deviation of the quantitative test results far exceeds the industry allowable range for clinical and food safety testing. The repeatability and accuracy of the test cannot meet compliance requirements. At the same time, areas not fully wetted by the reagent are prone to retaining unreacted impurities and microorganisms, causing uneven background signals and increased non-specific adsorption, further increasing the false positive rate. This seriously restricts the detection reliability of such devices and their implementation in grassroots field applications. Summary of the Invention

[0006] The technical problem to be solved by this invention is that existing microfluidic detection devices for filter membrane enrichment generally suffer from uneven wetting of detection reagents, insufficient microbial reaction, low detection sensitivity, large result deviation, and easy occurrence of false positives and false negatives. To address this, we propose a microfluidic microbial enrichment detection device with a filter membrane capture layer.

[0007] To achieve the above objectives, this application adopts the following technical solution: a microfluidic microbial enrichment and detection device with a filter membrane capture layer, comprising an outer casing and a sample inlet tube fixedly installed at its upper end, wherein an enrichment and detection component is provided inside the outer casing; The enrichment detection assembly includes a connecting pipe fixedly connected to the inner wall of the outer casing. A microflow inlet pipe is symmetrically installed at the lower end of the connecting pipe. A detection box is fixedly installed at the end of the microflow inlet pipe opposite to the connecting pipe. A filter membrane plate is rotatably installed inside the detection box. A micro servo motor is fixedly installed on the outer side of the detection box. The drive end of the micro servo motor is fixedly connected to the filter membrane plate through a coupling. A cavity plate is rotatably installed in the middle of the inner side of the filter membrane plate. A filter membrane corner plate is fixedly installed in the middle of the lower part of the filter membrane plate. A first arc toothed rail is fixedly installed on the outer side of the filter membrane plate. A first gear is rotatably installed on the outer side of the detection box. The first gear is meshed with the first arc toothed rail and the cavity plate. Optical detection windows are symmetrically arranged on the inner wall of the detection box. A second gear is rotatably mounted on the outside of the testing box, and a hollow cylinder is slidably mounted on the inside of the testing box. A second arc toothed rail is fixedly mounted on the outside of the hollow cylinder. The second gear is meshed with both the first and second arc toothed rails.

[0008] Preferably, the rotation axes of the filter membrane plate, cavity plate, filter membrane corner plate, first arc toothed rail, hollow cylinder, and second arc toothed rail are aligned.

[0009] Preferably, the outer sides of both the filter membrane plate and the filter membrane corner plate are tightly fitted to the inner wall of the testing chamber.

[0010] Preferably, limit plates are fixedly installed at both ends of the hollow cylinder, and the limit plates are located inside the detection box.

[0011] Preferably, a drive belt is fitted on the outer side of the filter membrane plate, and the drive belt engages with the filter membrane plate.

[0012] Preferably, the filter membrane corner plate is located between two sets of optical detection windows. The optical detection windows include a light source module and a signal acquisition module, and the light source module and the signal acquisition module are arranged coaxially opposite each other.

[0013] Preferably, a micro-outlet tube is fixedly installed at the end of the detection box away from the micro-inlet tube, and a sample discharge tube is fixedly installed on the inner side of the outer box, with the micro-outlet tube connected to the sample discharge tube.

[0014] Preferably, a groove is provided on the inner side of the outer casing, and a filter plate is slidably installed on the inner side of the groove. The filter plate is magnetically attracted to the outer casing.

[0015] Preferably, multiple sets of sample injection tubes and enrichment detection components are provided, and they have the same structural composition.

[0016] The technical effects and advantages of this invention are as follows: By driving the filter membrane plate to tilt and rotate using a micro servo motor, combined with the reverse flow obstruction and diversion liquid inlet design of the hollow cylinder, the drawbacks of the traditional single-center direct liquid inlet are eliminated. This ensures that the detection reagent evenly covers and washes the entire filter membrane, allowing the microorganisms trapped on the filter membrane surface to fully react specifically with the reagents, effectively improving the detection signal response value, reducing the risk of false negatives in low-abundance samples, reducing detection errors, and significantly improving the reliability and application value of the microfluidic microbial enrichment detection device. Attached Figure Description

[0017] The disclosure of this invention is illustrated with reference to the accompanying drawings. It should be understood that the drawings are for illustrative purposes only and are not intended to limit the scope of protection of this invention. In the drawings, the same reference numerals are used to refer to the same parts: Figure 1 This is a top view of the overall external structure of the device of the present invention. Figure 2 This is a top view of the rear of the overall external structure of the device of the present invention, with the filter plate removed. Figure 3 This is an exploded view of the outer casing and sample inlet tube structure of the present invention; Figure 4 This is a front view of the external structure of the enrichment detection component of the present invention; Figure 5 This is a schematic diagram of the back of the external structure of the enrichment detection component of the present invention; Figure 6This is a schematic diagram of the detection box and its internal structure according to the present invention; Figure 7 This is a top view of the internal structure of the detection box of the present invention; Figure 8 This is an exploded view of the internal structure of the detection box of the present invention; Figure 9 This is a schematic diagram of the detection box and its internal structure according to the present invention; Figure 10 For the present invention Figure 9 Enlarged schematic diagram of the structure at point A in the middle.

[0018] Legend: 1. Outer casing; 2. Sample inlet tube; 3. Sample outlet tube; 4. Slide groove; 5. Filter plate; 6. Enrichment detection component; 61. Connecting tube; 62. Microflow inlet tube; 63. Detection box; 631. First gear; 632. Second gear; 633. Optical detection window; 64. Microflow outlet tube; 65. Transmission belt; 66. Filter membrane plate; 661. Cavity plate; 662. Filter membrane corner plate; 663. Micro servo motor; 664. First arc toothed track; 67. Hollowed-out cylinder; 671. Second arc toothed track; 672. Limiting plate. Detailed Implementation

[0019] It is readily understood that, based on the technical solution of this invention, those skilled in the art can propose various interchangeable structural methods and implementations without altering the essential spirit of the invention. Therefore, the following detailed embodiments and accompanying drawings are merely illustrative examples of the technical solution of this invention and should not be considered as the entirety of the invention or as limitations or restrictions on the technical solution of this invention.

[0020] According to one embodiment of the present invention, Figures 1 to 9 As shown.

[0021] The existing single-center direct-inlet flow channel layout of the device causes the reconstituted test reagent to only react effectively on the surface of the central inlet area of ​​the filter membrane, failing to uniformly wet the enrichment area of ​​the filter membrane. This results in the target microorganisms trapped on the filter membrane surface not being able to undergo sufficient specific recognition and reaction with the test reagent, causing a significant attenuation of the subsequent detection signal response value, a significant decrease in detection sensitivity, and a high likelihood of false negative results for low-abundance test samples. Furthermore, the large differences in the degree of reaction in different areas of the filter membrane lead to a relative standard deviation of quantitative detection results far exceeding the industry-permissible range for clinical and food safety testing. The repeatability and accuracy of the detection cannot meet compliance requirements. At the same time, areas not fully wetted by the reagent are prone to retaining unreacted impurities and microorganisms, causing uneven background signals and increased non-specific adsorption, further increasing the false positive rate. This seriously restricts the detection reliability and field application of this type of device. To solve this problem, the present invention incorporates the following design in a microfluidic microbial enrichment detection device with a filter membrane capture layer: A microfluidic microbial enrichment and detection device with a filter membrane capture layer includes an outer box 1 and a sample inlet tube 2 fixedly installed at its upper end. An enrichment and detection component 6 is provided inside the outer box 1. The enrichment detection component 6 includes a connecting pipe 61 fixedly connected to the inner wall of the outer casing 1. A microflow inlet pipe 62 is symmetrically installed at the lower end of the connecting pipe 61. A detection box 63 is fixedly installed at the end of the microflow inlet pipe 62 opposite to the connecting pipe 61. A filter membrane plate 66 is rotatably installed inside the detection box 63. A micro servo motor 663 is fixedly installed on the outer side of the detection box 63. The drive end of the micro servo motor 663 is fixedly connected to the filter membrane plate 66 through a coupling. A cavity plate 661 is rotatably installed in the middle of the inner side of the filter membrane plate 66. A filter membrane corner plate 662 is fixedly installed in the middle of the lower part of the filter membrane plate 66. A first arc toothed rail 664 is fixedly installed on the outer side of the filter membrane plate 66. A first gear 631 is rotatably installed on the outer side of the detection box 63. The first gear 631 is meshed with the first arc toothed rail 664 and the cavity plate 661. Optical detection windows 633 are symmetrically arranged on the inner wall of the detection box 63. The filter membrane corner plate 662 is located between two sets of optical detection windows 633. The optical detection window 633 includes a light source module and a signal acquisition module, and the light source module and the signal acquisition module are arranged coaxially opposite each other. Furthermore, the rotation axes of the filter membrane plate 66, the cavity plate 661, the filter membrane corner plate 662, the first arc toothed rail 664, the hollow cylinder 67, and the second arc toothed rail 671 are consistent.

[0022] First, the sample to be tested is filled into the injection tube 2. Then, the device is started, and the sample to be tested in the injection tube 2 flows through the connecting tube 61, the microfluidic inlet tube 62, and the detection chamber 63 in sequence under the action of gravity. During the process of the sample to be tested flowing through the detection chamber 63, it is filtered by the filter membrane plate 66, thereby trapping and enriching the microorganisms in the sample on the surface of the filter membrane plate 66, completing the concentrated capture of microorganisms. The enrichment process is continuous and stable, laying a reliable foundation for the full reaction of subsequent detection reagents and optical quantitative detection.

[0023] After a period of filtration, a microbial trapping layer forms on the surface of the filter membrane plate 66. At this point, lyophilized reagents containing the active detection components can be added to the injection tube 2, allowing them to flow with the sample and gradually dissolve. Simultaneously, the micro servo motor 663 is activated to rotate the filter membrane plate 66 by a certain angle until it is tilted. During this rotation, the filter membrane plate 66 synchronously drives the first arc toothed track 664 to rotate. Utilizing the meshing relationship between the track 664 and the first gear 631, as well as the meshing relationship between the first gear 631 and the cavity plate 661, the cavity plate 661 is simultaneously rotated. After the filter membrane plate 66 completes the tilting transition, the cavity plate 661 simultaneously opens, establishing spatial communication between the inner side of the filter membrane corner plate 662 and the surface of the filter membrane plate 66. This allows the sample containing the reagent to flow through the membrane plate for an extended period of time. In this process, the surface of the inclined filter plate 66 is scourted, thereby peeling off one half of the microbial capture layer formed by the previously filtered sample on the surface of the filter plate 66. Under the dual guidance of the inner arc surface of the cavity plate 661 and the inner inclined surface of the filter corner plate 662, the microorganisms are transferred to the inner side of the filter corner plate 662. After a period of time, by controlling the micro servo motor 663 to drive the filter plate 66 to rotate in the opposite direction by a certain angle, the other half of the microbial capture layer on the surface of the filter plate 66 can be peeled off. Similarly, under the dual guidance of the inner arc surface of the cavity plate 661 and the inner inclined surface of the filter corner plate 662, the microorganisms are transferred to the inner side of the filter corner plate 662. Under the combined action of fluid scouring and gravity sedimentation, the microorganisms on the inner side of the filter corner plate 662 can be concentrated in the bottom area, ultimately achieving the fixed-point concentrated secondary enrichment of dispersed enriched microorganisms.

[0024] Furthermore, by using samples containing the active detection components to flush and peel off the microbial capture layer on the tilted filter plate 66, a certain effect is achieved in terms of reagent wetting uniformity. On the one hand, the tilted filter plate 66 overcomes the drawbacks of local reagent accumulation and lack of reagent contact at the edges caused by the traditional horizontal placement of filter membranes. This allows the reconstituted reagent to fully spread and cover the microbial capture layer on the surface of the filter plate 66, without any wetting blind spots. Combined with flushing and peeling, this ensures that all microorganisms trapped on the surface of the filter plate 66 can fully contact the reagent and undergo specific reactions, effectively improving the uniformity of reagent wetting and the sufficiency of the reaction. This avoids problems such as insufficient reaction and detection signal deviation caused by reagent deficiency, ensuring the sensitivity and accuracy of subsequent detection.

[0025] Subsequently, after the microorganisms have completely collected inside the filter membrane corner plate 662 and fully reacted with the detection reagent, the optical detection windows 633 symmetrically arranged on the inner wall of the detection chamber 63 are precisely aligned with the concentrated microbial enrichment area of ​​the filter membrane corner plate 662. The external optical detection component emits a stable light source through one optical detection window 633. The light passes perpendicularly through the reagent reaction area and the collected microbial layer, and then completes the reception and acquisition of transmitted light or characteristic signals through the other optical detection window 633. Relying on the differences in transmittance, color development, or fluorescence signals generated by the specific reaction between microorganisms and detection reagents, the changes in optical parameters are captured in real time, and the light signals are converted into analyzable detection data to determine the presence and content of microorganisms in the sample, thereby completing a precise in-situ optical detection operation.

[0026] Furthermore, a second gear 632 is rotatably mounted on the outside of the detection chamber 63, and a perforated cylinder 67 is slidably mounted on the inside of the detection chamber 63. A second arc toothed rail 671 is fixedly mounted on the outside of the perforated cylinder 67. The second gear 632 is meshed with both the first arc toothed rail 664 and the second arc toothed rail 671. As the first arc toothed rail 664 rotates in both directions following the filter membrane plate 66, the meshing transmission of the second gear 632 synchronously drives the perforated cylinder 67 to rotate at a certain angle in both directions. This causes a portion of the perforated cylinder 67 to enter the interior of the detection chamber 63, thereby creating a certain obstruction to the internal flow channel of the detection chamber 63. Since the synchronous rotation direction of the filter membrane plate 66 and the perforated cylinder 67 is opposite, the sample containing reagents falls from the upper half of the filter membrane plate 66 at a higher horizontal height, precisely flushing the microbial capture layer in that area. This further enhances the sufficient contact between the reagent and the microorganisms on the surface of the filter membrane plate 66, laying a solid foundation for the subsequent accurate signal acquisition by the optical detection window 633 and improving the detection accuracy.

[0027] In addition, the outer sides of the filter membrane plate 66 and the filter membrane corner plate 662 are tightly fitted to the inner wall of the detection chamber 63. Limiting plates 672 are fixedly installed at both ends of the hollow cylinder 67. The limiting plates 672 are located inside the detection chamber 63. The tight fit between the filter membrane plate 66, the filter membrane corner plate 662, and the inner wall of the detection chamber 63 effectively seals side gaps, preventing lateral flow of reagent-containing samples and leakage from gaps. It also prevents fluid from flowing away directly without sufficient contact with the surface of the filter membrane plate 66, ensuring that the fluid flows along a preset path and washes the filter membrane plate 66. Furthermore, the limiting plates 672 constrain and limit the sliding stroke and rotational limits of the hollow cylinder 67, preventing displacement, overtravel, or structural jamming.

[0028] A transmission belt 65 is fitted on the outer side of the filter membrane plate 66, and the transmission belt 65 meshes with the filter membrane plate 66. A micro-outlet tube 64 is fixedly installed at the end of the detection box 63 opposite to the micro-flow inlet tube 62. A sample discharge tube 3 is fixedly installed on the inner side of the outer box 1. The micro-outlet tube 64 is connected to the sample discharge tube 3. Multiple sets of sample inlet tube 2 and enrichment detection components 6 are provided with the same structure. Through the setting of the transmission belt 65 and the multiple sets of sample inlet tube 2 and enrichment detection components 6, multiple samples to be tested can be processed at the same time, which greatly improves the detection throughput and adapts to the batch detection needs of grassroots sites. It can also realize multiple parallel detections of the same sample, effectively reduce detection errors, improve the repeatability of detection results, further expand the detection range and practicality of the device, and take into account both detection efficiency and detection reliability.

[0029] By connecting the micro-outflow tube 64 to the sample discharge tube 3, excess reagents, waste liquids and impurities in the detection chamber 63 after microbial flushing and reagent reaction can be discharged in a timely manner, avoiding the accumulation of waste liquid that could cause internal contamination and flow channel blockage in the detection chamber 63. At the same time, it maintains smooth internal fluid flow, providing a clean and stable environment for uniform reagent spreading and full microbial reaction.

[0030] Secondly, a slid groove 4 is provided on the inner side of the outer box 1, and a filter plate 5 is slidably installed on the inner side of the slid groove 4. The filter plate 5 is magnetically attracted to the outer box 1. The filter plate 5 can complete the pre-filtration before the sample enters the enrichment and detection component 6, effectively intercepting large particulate impurities, tissue debris and aggregates in the sample, preventing coarse particles from entering the microfluidic inlet tube 62 and the detection box 63, preventing microchannel blockage and premature contamination and blockage of the filter membrane plate 66, reducing non-specific adsorption interference caused by impurities. At the same time, the detachable magnetic filter plate 5 is easy to quickly disassemble, clean and replace later. The structure is simple and the maintenance is convenient, effectively improving the overall service life of the device and the adaptability to complex matrix samples.

[0031] Working principle: First, the sample to be tested is injected into the sample inlet tube 2. Then, the sample passes through the filter plate 5, which is magnetically installed in the inner groove 4 of the outer box 1, to complete the pre-filtration. After pretreatment, the sample flows through the connecting tube 61 and the micro-flow inlet tube 62 in sequence under the action of gravity and is sent into the detection box 63. It is then fully filtered by the filter membrane plate 66, so that the target microorganisms in the sample are stably trapped and enriched on the surface of the filter membrane plate 66, forming a uniform microbial capture layer.

[0032] After the first stage of enrichment is completed, lyophilized reagent containing the active detection component is added to the injection tube 2. The lyophilized reagent is gradually re-dissolved and mixed with the fluid flow to form a mixture containing the detection reagent. At the same time, the micro servo motor 663 is started to drive the filter membrane plate 66 to rotate synchronously and tilt. During the rotation of the filter membrane plate 66, the first arc toothed rail 664 is driven to mesh with the first gear 631, which in turn drives the cavity plate 661 to rotate and open synchronously, so that the inner space of the filter membrane corner plate 662 is connected to the surface of the filter membrane plate 66. Meanwhile, the first arc toothed track 664 meshes with the second gear 632 to drive the second arc toothed track 671, causing the hollow cylinder 67 to rotate in the opposite direction and partially block the internal flow channel of the detection box 63, changing the fluid flow direction and forcing the reagent-containing mixture to fall from the high-position filter membrane plate 66 area, rinsing the surface of the inclined filter membrane plate 66, peeling off the microbial capture layer on both sides of the filter membrane plate 66 in sequence, and then through the synergistic guiding effect of the inner arc surface of the cavity plate 661 and the inner inclined surface of the filter membrane corner plate 662, combined with the fluid rinsing and gravity sedimentation, the dispersed and trapped microorganisms are uniformly collected to the bottom of the inner side of the filter membrane corner plate 662, realizing the concentrated secondary enrichment of microorganisms and the full specific reaction of the detection reagent.

[0033] After the biochemical reaction is complete, the optical detection windows 633 symmetrically arranged on the inner wall of the detection chamber 63 are precisely aligned with the microbial collection area. The light source module of one optical detection window 633 emits detection light vertically. After the light penetrates the reaction area and the microbial enrichment layer, the light signal is received by the signal acquisition module on the other side. Based on the differences in color development, light transmission or fluorescence signals, in-situ optical detection of microorganisms is completed for qualitative and quantitative analysis.

[0034] After the test is completed, the waste liquid, residual reagents and impurities can be uniformly discharged into the sample discharge tube 3 through the micro-outflow tube 64 to ensure that the internal flow channel of the test box 63 is clean and unobstructed.

[0035] The technical scope of this invention is not limited to the content described above. Those skilled in the art can make various modifications and variations to the above embodiments without departing from the technical concept of this invention, and all such modifications and variations should fall within the protection scope of this invention.

Claims

1. A microfluidic microbial enrichment and detection device with a filter membrane trapping layer, characterized in that: It includes an outer box (1) and a sample inlet tube (2) fixedly installed at its upper end. An enrichment detection component (6) is provided inside the outer box (1). The enrichment detection component (6) includes a connecting pipe (61) fixedly connected to the inner wall of the outer casing (1). A microflow inlet pipe (62) is symmetrically installed at the lower end of the connecting pipe (61). A detection box (63) is fixedly installed at the end of the microflow inlet pipe (62) opposite to the connecting pipe (61). A filter membrane plate (66) is rotatably installed on the inner side of the detection box (63). A micro servo motor (663) is fixedly installed on the outer side of the detection box (63). The drive end of the micro servo motor (663) is connected to the filter membrane plate (66) via a coupling. 6) Fixed connection: A cavity plate (661) is rotatably installed in the middle of the inner side of the filter membrane plate (66), a filter membrane corner plate (662) is fixedly installed in the middle of the lower part of the filter membrane plate (66), a first arc toothed rail (664) is fixedly installed in the outer side of the filter membrane plate (66), a first gear (631) is rotatably installed in the outer side of the detection box (63), the first gear (631) is meshed with the first arc toothed rail (664) and the cavity plate (661), and optical detection windows (633) are symmetrically arranged on the inner wall of the detection box (63). A second gear (632) is rotatably mounted on the outside of the detection box (63), and a hollow cylinder (67) is slidably mounted on the inside of the detection box (63). A second arc toothed rail (671) is fixedly mounted on the outside of the hollow cylinder (67). The second gear (632) is meshed with both the first arc toothed rail (664) and the second arc toothed rail (671).

2. The microfluidic microbial enrichment and detection device with a filter membrane capture layer according to claim 1, characterized in that: The rotation axes of the filter membrane plate (66), cavity plate (661), filter membrane corner plate (662), first arc toothed rail (664), hollow cylinder (67), and second arc toothed rail (671) are consistent.

3. The microfluidic microbial enrichment and detection device with a filter membrane capture layer according to claim 1, characterized in that: The outer sides of the filter membrane plate (66) and the filter membrane corner plate (662) are tightly fitted to the inner wall of the detection box (63).

4. The microfluidic microbial enrichment and detection device with a filter membrane capture layer according to claim 1, characterized in that: Both ends of the hollow cylinder (67) are fixedly installed with limiting plates (672), and the limiting plates (672) are located inside the detection box (63).

5. The microfluidic microbial enrichment and detection device with a filter membrane capture layer according to claim 1, characterized in that: A transmission belt (65) is sleeved on the outer side of the filter membrane plate (66), and the transmission belt (65) engages with the filter membrane plate (66).

6. The microfluidic microbial enrichment and detection device with a filter membrane capture layer according to claim 1, characterized in that: The filter membrane corner plate (662) is located between the two sets of optical detection windows (633). The optical detection window (633) includes a light source module and a signal acquisition module, and the light source module and the signal acquisition module are arranged coaxially opposite each other.

7. The microfluidic microbial enrichment and detection device with a filter membrane capture layer according to claim 1, characterized in that: A micro-outlet tube (64) is fixedly installed at the end of the detection box (63) away from the micro-inlet tube (62), and a sample discharge tube (3) is fixedly installed on the inner side of the outer box (1). The micro-outlet tube (64) is connected to the sample discharge tube (3).

8. The microfluidic microbial enrichment and detection device with a filter membrane capture layer according to claim 1, characterized in that: The inner side of the outer box (1) is provided with a sliding groove (4), and a filter plate (5) is slidably installed on the inner side of the sliding groove (4). The filter plate (5) is magnetically attracted to the outer box (1).

9. The microfluidic microbial enrichment and detection device with a filter membrane capture layer according to claim 1, characterized in that: The sample inlet tube (2) and the enrichment detection component (6) are provided in multiple sets and have the same structure.

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