Method for constructing an engineered bacterium for synthesizing fatty acids and derivatives thereof and application thereof

By enhancing the homologous recombination ability of Kluyveromyces martensii and modifying it with CRISPR-Cas9, an engineered strain capable of efficiently synthesizing fatty acids and their derivatives was constructed. This solved the problems of limitations in gene editing tools and the cost of adding exogenous oleic acid, and enabled efficient production and high yield at high temperatures.

CN122189054APending Publication Date: 2026-06-12DALIAN UNIV OF TECH
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Applications(China)
Current Assignee / Owner
DALIAN UNIV OF TECH
Filing Date
2024-12-12
Publication Date
2026-06-12

AI Technical Summary

Technical Problem

In the existing technology, the development of gene editing tools for Kluyveromyces martensii is limited, which restricts its potential as a chassis cell for chemical production. Furthermore, the synthesis of fatty acids and their derivatives requires the addition of exogenous oleic acid, which increases costs and results in low oleic acid conversion efficiency.

Method used

By enhancing the homologous recombination ability of Kluyveromyces martensii and using the CRISPR-Cas9 gene editing tool to modify its fatty acid metabolism pathway, including inactivating specific genes and integrating exogenous enzymes, an engineered strain capable of efficiently synthesizing fatty acids and their derivatives was constructed.

Benefits of technology

It enables the efficient synthesis of various fatty acids and their derivatives under high-temperature conditions, reducing production costs and increasing yield and conversion efficiency. It is applicable to the fields of daily chemicals, polymer monomers, food and feed.

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Abstract

The present application belongs to the field of microbial synthetic biology and metabolic engineering technology, and particularly relates to an engineered Kluyveromyces marxianus strain for synthesizing fatty acids and derivatives thereof, a construction method and application thereof. The initial strain is Kluyveromyces marxianus, and the homologous recombination ability of the initial strain is enhanced to obtain a chassis strain which is easy to be modified by metabolic engineering. Then, the gene editing tool is optimized to modify the fatty acid metabolic pathway of the initial strain, and thus the engineered strain for synthesizing fatty acids and derivatives thereof is obtained. The gene editing tool is optimized, and the tool is used to construct the engineered strain for high-yield of fatty acids and derivatives thereof by taking the Kluyveromyces marxianus which is resistant to high temperature and can uptake various carbon sources as the chassis cell. The engineered strain can be applied to the biosynthesis and manufacturing of various fatty acid derivatives.
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