Method for counting viable bacteria in probiotic compositions containing bifidobacteria and lactobacilli

By combining dilution with a specific culture medium, the problem of inaccurate counts of Bifidobacterium and Lactobacillus in existing technologies has been solved, achieving higher detection accuracy and quality control.

CN122189149APending Publication Date: 2026-06-12MINSHENG ZHONGKE JIAYI (ZHEJIANG) BIOENGINEERING CO LTD
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Applications(China)
Current Assignee / Owner
MINSHENG ZHONGKE JIAYI (ZHEJIANG) BIOENGINEERING CO LTD
Filing Date
2026-03-20
Publication Date
2026-06-12

AI Technical Summary

⚠Technical Problem

Existing detection methods cannot accurately count the number of live bacteria in probiotic compositions containing Bifidobacterium and Lactobacillus, resulting in a large discrepancy between the test results and the theoretical values, which affects product quality control.

⚗Method used

The cells were serially diluted 10-fold using diluents and cultured in modified MRS and TOS media. The diluents, consisting of Tween 80, peptone, and L-cysteine ​​hydrochloride, were combined with temperature and homogenization conditions during the dilution process to ensure accurate viable cell counts.

🎯Benefits of technology

It significantly improves the accuracy of live counts of Bifidobacterium and Lactobacillus, making the test results closer to the theoretical values ​​and meeting the quality control requirements of Minsheng Puribao brand probiotic granules.

✦ Generated by Eureka AI based on patent content.

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Abstract

The application relates to a method for counting viable bacteria in a probiotic composition containing bifidobacterium and lactobacillus. The method comprises the following steps: S1, mixing the probiotic composition to be detected with a diluent to prepare a sample homogenate; then using the diluent to perform 10 times gradient dilution on the sample homogenate to prepare test solutions with different dilutions; S2, using a culture medium to perform pouring plate culture on 2-3 test solutions with continuous dilutions; S3, counting the colonies on the plates after culture, and calculating the viable bacteria number in the probiotic composition. Compared with the prior art, the bifidobacterium number and the lactobacillus number in the probiotic composition detected by the method are obviously improved, the accuracy of the detection result is improved, and the method can be well applied to the viable bacteria counting of the probiotic composition containing bifidobacterium and lactobacillus, especially the Minsheng Puerbao brand probiotic granules.
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