A continuous sampling device for sugar group detection

Abstract

The application relates to a continuous sampling device for sugar group detection, and belongs to the technical field of sugar group detection equipment. The structure of the sampling device comprises a sampling unit and an execution unit. The sampling unit comprises a microdialysis probe and a first pipeline. One end of the microdialysis probe is arranged on the execution unit through the first pipeline. The other end of the microdialysis probe is a sharp end. The outermost layer of the microdialysis probe is a semi-permeable membrane. The inside of the microdialysis probe is a hollow cavity. The execution unit comprises an execution shell. A trapping assembly and an input assembly are arranged in the execution shell. The first pipeline is in communication with the trapping assembly and the input assembly respectively. A detection interface is arranged on one side of the execution shell in communication. The detection interface is in communication with the trapping assembly. A transfusion unit is arranged on one side of the execution shell in communication. The transfusion unit is in communication with the input assembly. The application has the technical effect of realizing stable continuous sugar group sample sampling and detection.

Description

Technical Field

[0001] This application relates to the technical field of glycosylation detection equipment, and in particular to a continuous sampling device for glycosylation detection. Background Technology

[0002] Glycomics is an important research direction that has emerged in the life sciences after genomics and proteomics. The expression, structure and distribution of glycomic substances are closely related to the physiological functions of the body and the occurrence and development of diseases, and are of great significance for the early diagnosis of diseases and the study of pathological mechanisms.

[0003] In the dynamic monitoring and analysis of glycomic substances, devices capable of minimally invasive, real-time, and continuous sampling are needed to obtain stable and pure test samples. Microdialysis technology, with its advantages of selective permeability of semi-permeable membranes, in-situ sampling, and minimal interference with samples, has been widely used in the online sampling and enrichment of glycomic substances, becoming a key front-end technology in the field of glycomic detection. Currently, most common continuous sampling devices for glycomic detection adopt a split structure, with sampling, enrichment, and flow path switching functions set up independently. These modules work together through external pipelines and control components. However, it is difficult to achieve automatic connection between the various stages during use, which can easily lead to problems such as sample loss, cumbersome operation, and poor stability during continuous operation.

[0004] Regarding the aforementioned technologies, the inventors believe that there are shortcomings in achieving stable and reliable continuous glycoside sample collection and detection. Summary of the Invention

[0005] To address the aforementioned technical problems, this application provides a continuous sampling device for glycosylation detection.

[0006] This application provides a continuous sampling device for glycosylation detection, which adopts the following technical solution: A continuous sampling device for glycosylation detection includes a sampling unit and an execution unit. The sampling unit includes a microdialysis probe and a first conduit. One end of the microdialysis probe is disposed on the execution unit via the first conduit. The other end of the microdialysis probe is a tip, and the outermost layer of the microdialysis probe is a semi-permeable membrane. The interior of the microdialysis probe is a hollow cavity. The execution unit includes an execution housing, inside which a trapping component and an input component are disposed. The first conduit is connected to both the trapping component and the input component. A detection interface is disposed on one side of the execution housing, and the detection interface is connected to the trapping component. An infusion unit is disposed on one side of the execution housing, and the infusion unit is connected to the input component.

[0007] By adopting the above technical solution, the sampling unit employs a microdialysis probe design. Its tip structure facilitates precise insertion into the sample, while the outermost semi-permeable membrane selectively allows small-molecule glycosides to pass through, effectively intercepting large-molecule impurities in the sample and preventing interference with subsequent detection. Simultaneously, the hollow cavity provides a stable channel for the mixing and transport of saline and glycosides, ensuring the purity and accuracy of the sample. The first pipeline connects both the trapping and input components, achieving integrated connectivity between the sampling and execution units. This simplifies the overall piping layout, reduces the risk of leakage at pipe connections, and ensures the continuity of saline delivery and glycoside sample transfer, thus providing a stable and reliable sampling solution for the device. Continuous sampling is supported by a structural design. The execution unit adopts a structure that integrates the collection and input components into the execution shell, making it easy to carry and install. At the same time, the shell protects the internal components from external environmental interference, ensuring the stability and service life of the components. The detection interface is directly connected to the collection component, simplifying the sample detection process, facilitating rapid connection to external detection equipment, reducing loss and contamination during sample transfer, and improving detection efficiency. The infusion unit is directly connected to the input component, enabling a continuous and stable supply of physiological saline, providing stable power support for microdialysis sampling and sample enrichment, and ensuring the smooth operation of continuous sampling.

[0008] Preferably, the first conduit is provided with an inlet tube and an outlet tube, one end of the inlet tube and one end of the outlet tube are disposed in the hollow cavity of the microdialysis probe; the inlet tube is disposed in the execution housing through the first conduit, and the other end of the inlet tube is connected to the input component; the outlet tube is disposed in the execution housing through the first conduit, and the other end of the outlet tube is connected to the collection component.

[0009] By adopting the above technical solution, the first pipeline adopts a dual-pipeline structure with an independent inlet pipe and an outlet pipe. Both pipelines are integrated into the first pipeline and extend to the microdialysis probe cavity and the execution housing, respectively, to achieve separate delivery of saline input and sample output without interference, avoiding fluid mixing and sample dilution. The inlet pipe is connected to the input component and the outlet pipe is connected to the collection component, forming a stable closed-loop flow path, improving sampling continuity and reliability, while simplifying the overall pipeline layout, reducing assembly difficulty, and improving the operational stability of the device.

[0010] Preferably, the collection assembly includes a collection tank and an extraction pump. One end of the extraction pump is connected to the top of the collection tank, and the other end of the extraction pump is connected to an outlet pipe. Porous graphitized carbon is disposed inside the collection tank. An inlet is provided on one side of the upper part of the collection tank, and the inlet is connected to a detection interface. A detection port is provided at the bottom of the collection tank, and the detection port penetrates the execution housing and is located outside the execution housing. A drain outlet is provided on one side of the lower part of the collection tank, and the drain outlet penetrates the execution housing.

[0011] By adopting the above technical solution, the extraction pump is connected at one end to the outlet pipe and at the other end to the top of the collection tank, providing stable extraction power. This allows the saline solution carrying the glycoside sample in the outlet pipe to be drawn into the collection tank, preventing sample retention or leakage during transport and ensuring the continuity and stability of sample delivery. This provides a reliable foundation for subsequent sample enrichment. The porous graphitized carbon inside the collection tank has excellent adsorption properties, specifically targeting and enriching small molecules of the glycosides, effectively concentrating the glycoside components in the sample, lowering the detection limit, and significantly improving detection sensitivity and accuracy. The inlet on one side of the upper part of the collection tank is directly connected to the detection interface, allowing for rapid connection to external detection equipment and improving detection efficiency. Simultaneously, to ensure rapid and uniform entry of subsequent flushing solution into the collection tank for efficient elution of the glycoside sample, the detection port at the bottom of the collection tank directly connects to the external detection device, and the drain outlet promptly discharges waste liquid generated during the collection process, preventing waste liquid accumulation in the tank and causing blockage or pressure imbalance.

[0012] Preferably, the input component includes an input tube body, a rotating shaft, a turbine, and a push pump; one end of the rotating shaft is disposed inside the input tube body, and the other end of the rotating shaft is disposed outside the input tube body, and the rotating shaft is rotatably connected to one end of the input tube body; one end of the push pump is connected to one end of the input tube body, and the other end of the push pump is connected to the inlet pipe; the other end of the input tube body is connected to the infusion unit; the turbine is located inside the input tube body, and the turbine is fixedly disposed at one end of the rotating shaft; the rotating shaft located outside the input tube body is provided with threads.

[0013] By adopting the above technical solution, the input tube, rotating shaft, turbine, and push pump work together to drive the turbine to rotate using fluid flow, converting fluid power into rotation of the rotating shaft. The push pump is connected to both the input tube and the inlet tube, enabling stable delivery of saline solution to the microdialysis probe, ensuring uniform flow rate and stable velocity of the dialysate, and improving the repeatability and accuracy of sampling. The other end of the input tube is connected to the infusion unit, forming a continuous fluid supply path, ensuring uninterrupted supply of saline solution and providing a stable environment for microdialysis sampling. The turbine is fixed to one end of the rotating shaft and can synchronously drive the rotating shaft to rotate as saline solution flows through, effectively converting fluid energy into rotational power. The rotating shaft is externally threaded, which converts rotational motion into linear motion of subsequent structures, realizing power transmission and motion conversion, providing timing control for valve switching, ensuring automatic switching between enrichment and detection processes, and improving the overall automation and operational consistency of the device.

[0014] Preferably, a switching valve is provided on the top of the collection tank; the switching valve includes a ball valve, a valve shaft, a valve stem, and a torsion spring. The ball valve is rotatably mounted inside the collection tank, and a through hole is provided on the ball valve. A detection inlet is provided on one side of the ball valve, and the detection inlet communicates with the through hole; one end of the valve shaft is fixedly mounted on the ball valve, and the other end of the valve shaft is located outside the collection tank, and the valve shaft is rotatably connected to the collection tank; one end of the valve stem is fixedly mounted on the other end of the valve shaft; the torsion spring is sleeved on the other end of the valve shaft, one end of the torsion spring is fixedly mounted on the collection tank, and the other end of the torsion spring is fixedly mounted on the valve stem; a limit groove is provided on one side of the valve stem.

[0015] By adopting the above technical solution, the switching valve realizes the switching between enrichment and detection states. The ball valve is rotated and installed in the collection tank, with the through hole connected to the detection inlet. In the enrichment state, it ensures that the sample-carrying saline flows smoothly into the collection tank for adsorption and enrichment. In the detection state, the rotation of the ball valve quickly opens the flushing liquid passage, realizing efficient connection between sample elution and delivery for testing, adapting to the continuous operation requirements of the device and improving sample processing efficiency. The torsion spring is sleeved on the outside of the valve shaft, with both ends fixed to the collection tank and the valve stem respectively, which can provide a stable reset force for the valve stem, realizing automatic reset of the switching valve state. A limiting groove is provided on one side of the valve stem to limit the rotation angle of the valve stem, avoiding excessive rotation of the valve stem that causes the ball valve position to deviate, ensuring precise docking of the through hole, detection inlet and relevant interfaces of the collection tank, preventing sample loss, flushing liquid leakage or waste liquid backflow caused by docking deviation, ensuring the orderly operation of each link of enrichment, elution and delivery for testing, and further improving the stability and reliability of the device operation.

[0016] Preferably, a switching assembly is provided at the bottom of the collection tank; the switching assembly includes a switching ball, a switching shaft, a switching rod, and a connecting rod. The switching ball is rotatably disposed inside the collection tank, and a switching hole is provided through the switching ball. A waste liquid discharge hole is provided on one side of the switching ball, and the waste liquid discharge hole communicates with the switching hole. One end of the switching shaft is fixedly disposed on the switching ball, and the other end of the switching shaft is disposed outside the collection tank, and the switching shaft is rotatably connected to the collection tank. One end of the switching rod is fixedly disposed on the other end of the switching shaft. One end of the connecting rod is rotatably disposed on the other end of the switching rod, and the other end of the connecting rod is rotatably disposed on the other end of the valve stem.

[0017] By adopting the above technical solution, the switching component uses a combination structure of a switching ball, a switching shaft, a switching rod, and a connecting rod to achieve precise switching between waste liquid discharge and sample delivery. The connecting rod is linked with the top switching valve to ensure synchronized operation of both, improving the automation level and operational coordination of the device. The switching ball is rotatably located at the bottom of the collection tank, with a switching hole on the ball connected to the waste liquid discharge hole. In the enrichment state, the waste liquid discharge hole connects to the drain outlet to promptly discharge waste liquid and prevent accumulation and contamination. In the detection state, the switching hole connects to the delivery path, reducing sample retention and loss. The switching shaft precisely transmits rotational power to ensure smooth flow path switching, and the connecting rod achieves linkage control with the switching valve to ensure synchronized process timing.

[0018] Preferably, a drive assembly is provided inside the execution housing. The drive assembly includes a guide rail, a slider, a drive sleeve, a drive spring, and a drive rod. The guide rail is located on one side of the rotating shaft and is fixedly disposed on the inner wall of the execution housing. The slider is slidably disposed on the guide rail, and its middle portion is threaded onto the rotating shaft. One end of the drive sleeve is fixedly disposed on the slider, and the drive rod is slidably disposed inside the drive sleeve. The drive spring is disposed inside the drive sleeve, with one end fixedly disposed on the drive sleeve and the other end fixedly disposed on one end of the drive rod. The other end of the drive rod abuts against the switching valve.

[0019] By adopting the above technical solution, the drive assembly uses a combination structure of guide rail, slider, drive sleeve, drive spring and drive rod to ensure the stability and reliability of the device's enrichment and detection state switching. The guide rail is fixed to the inner wall of the execution housing to provide stable sliding guidance for the slider and prevent slider deviation. The slider is connected to the rotating shaft through threads, which can convert the rotational motion of the rotating shaft into linear motion. The drive sleeve moves synchronously with the slider, and the drive spring always applies elastic force to keep the drive rod pressed against the switching valve stem, which can accurately limit the initial position of the valve stem and ensure that the valve stem does not rotate during the enrichment stage, ensuring that sample enrichment proceeds smoothly. The drive rod is slidably set inside the drive sleeve, and the drive spring ensures the position change of the valve stem in the early stage.

[0020] Preferably, a spiral spring is sleeved on the other end of the rotating shaft, with one end of the outer ring of the spiral spring fixedly mounted on the input tube and one end of the inner ring of the spiral spring fixedly mounted on the rotating shaft.

[0021] By adopting the above technical solution, the spiral spring is sleeved on the other end of the rotating shaft, with the outer ring fixed to the input pipe and the inner ring fixed to the rotating shaft. When the rotating shaft rotates, it stores elastic potential energy. After the push pump stops working, it releases the elastic force to drive the rotating shaft to reset in the reverse direction, thereby driving the drive component, switching valve and switching component to reset synchronously. This enables all components of the device to automatically return to the initial enrichment state, ensuring that the next round of sampling and testing can be carried out in an orderly manner, and improving the automation and stability of the continuous operation of the device.

[0022] Preferably, the drain outlet is provided with a waste liquid unit, the waste liquid unit including a second pipe and a waste liquid bag; one end of the second pipe is connected to the drain outlet; the waste liquid bag is connected to the other end of the second pipe.

[0023] By adopting the above technical solution, the waste liquid unit uses a combination structure of a second pipe and a waste liquid bag. The structure is simple and easy to assemble and disassemble. One end of the second pipe is connected to the drain outlet and the other end is connected to the waste liquid bag. This enables the centralized collection and sealed storage of waste liquid, excess saline, and impurities during the collection process, avoiding environmental pollution caused by waste liquid leakage. At the same time, it prevents waste liquid from flowing back into the collection tank and contaminating the sample, ensuring sample purity and a clean operating environment for the device. The waste liquid bag can be flexibly replaced to adapt to the continuous sampling requirements of the device, ensuring smooth waste liquid discharge without accumulation. This avoids blockage of the drain outlet and pressure imbalance in the collection tank due to waste liquid retention, ensuring orderly connection between sample enrichment and waste discharge processes.

[0024] Preferably, the infusion unit includes a third conduit and an infusion bag, one end of the third conduit being connected to the other end of the input tube; the infusion bag is connected to the other end of the third conduit.

[0025] By adopting the above technical solution, the infusion unit adopts a combination structure of a third pipeline and an infusion bag, which is simple in structure and easy to assemble. One end of the third pipeline is connected to the input tube and the other end is connected to the infusion bag, which can realize the stable storage and continuous delivery of physiological saline, providing sufficient and stable fluid support for microdialysis sampling, sample enrichment and overall device operation, and ensuring uninterrupted continuous sampling. The infusion bag can be flexibly replaced and replenished with physiological saline.

[0026] In summary, this application includes at least one of the following beneficial technical effects: 1. The input tube works in conjunction with the rotating shaft, turbine, and push pump. Fluid flow drives the turbine's rotation, converting fluid power into the rotation of the rotating shaft. The push pump connects to both the input tube and the inlet tube, stably pushing physiological saline to the microdialysis probe, ensuring uniform flow rate and stable velocity, thus improving sampling repeatability and accuracy. The other end of the input tube connects to the infusion unit, forming a continuous fluid supply path, ensuring uninterrupted physiological saline supply and providing a stable environment for microdialysis sampling. The turbine, fixed to one end of the rotating shaft, synchronously drives the shaft's rotation as physiological saline flows through, effectively converting fluid energy into rotational power. Threads on the external rotating shaft convert rotational motion into linear motion for subsequent structures, achieving power transmission and motion conversion. This provides timing control for valve switching, ensuring automatic switching between enrichment and detection processes, and improving the overall automation and operational consistency of the device.

[0027] 2. A spiral spring is sleeved on the other end of the rotating shaft. The outer ring is fixed to the input pipe and the inner ring is fixed to the rotating shaft. When the rotating shaft rotates, it stores elastic potential energy. After the push pump stops working, it releases the elastic force to drive the rotating shaft to reset in the opposite direction. This, in turn, drives the drive assembly, switching valve and switching assembly to reset synchronously, so that each component of the device automatically returns to the initial enrichment state, ensuring that the next round of sampling and testing can be carried out in an orderly manner, and improving the automation and stability of the continuous operation of the device. Attached Figure Description

[0028] Figure 1 This is a schematic diagram of the overall structure in the embodiment.

[0029] Figure 2 This is a cross-sectional schematic diagram of the internal structure of the microdialysis probe in the embodiment.

[0030] Figure 3 This is a cross-sectional schematic diagram of the internal structure of the casing in the embodiment.

[0031] Figure 4 This is a cross-sectional schematic diagram of the internal structure of the trapping tank in the embodiment.

[0032] Figure 5 This is a cross-sectional schematic diagram of the internal structure of the input tube in the embodiment.

[0033] Explanation of reference numerals in the attached drawings: 1. Sampling unit; 11. Microdialysis probe; 111. Semi-permeable membrane; 112. Hollow cavity; 12. First pipeline; 121. Inlet pipe; 122. Outlet pipe; 2. Actuation unit; 21. Actuation housing; 211. Detection interface; 22. Collection assembly; 221. Collection tank; 2211. Inlet; 2212. Detection port; 2213. Drain outlet; 222. Extraction pump; 223. Porous graphitized carbon; 23. Input assembly; 231. Input tube; 232. Rotating shaft; 233. Turbine; 234. Spiral spring; 235. 3. Push pump; 4. Switching valve; 5. Ball valve; 6. Through hole; 7. Detection inlet; 8. Valve shaft; 9. Valve stem; 10. Limiting groove; 11. Torsion spring; 12. Switching assembly; 13. Switching ball; 14. Switching hole; 15. Waste liquid discharge hole; 16. Switching shaft; 27. Switching rod; 38. Connecting rod; 49. Drive assembly; 20. Guide rail; 20. Slider; 31. Drive sleeve; 42. Drive spring; 53. Drive rod; 64. Waste liquid unit; 75. Second pipeline; 86. Waste liquid bag; 97. Infusion unit; 10. Third pipeline; 11. Infusion bag. Detailed Implementation

[0034] The following is in conjunction with the appendix Figure 1-5 This application will be described in further detail.

[0035] This application discloses a continuous sampling device for glycosylation detection. (Refer to...) Figure 1 , Figure 2 and Figure 3 The system includes a sampling unit 1 and an execution unit 2. The sampling unit 1 includes a microdialysis probe 11 and a first conduit 12. One end of the microdialysis probe 11 is mounted on the execution unit 2 via the first conduit 12. The other end of the microdialysis probe 11 is a tip, and the outermost layer of the microdialysis probe 11 is a semi-permeable membrane 111. The interior of the microdialysis probe 11 is a hollow cavity 112. The execution unit 2 includes an execution housing 21, inside which a collection component 22 and an input component 23 are disposed. The first conduit 12 is connected to the collection component 22 and the input component 23, respectively. A detection interface 21 is provided on one side of the execution housing 21. 1. The detection interface 211 is connected to the collection component 22; an infusion unit 7 is provided on one side of the execution housing 21, and the infusion unit 7 is connected to the input component 23; an inlet pipe 121 and an outlet pipe 122 are provided in the first pipeline 12, and one end of the inlet pipe 121 and one end of the outlet pipe 122 are located in the hollow cavity 112 of the microdialysis probe 11; the inlet pipe 121 is located in the execution housing 21 through the first pipeline 12, and the other end of the inlet pipe 121 is connected to the input component 23; the outlet pipe 122 is located in the execution housing 21 through the first pipeline 12, and the other end of the outlet pipe 122 is connected to the collection component 22.

[0036] The infusion unit 7 continuously delivers saline solution to the inlet tube 121 via the input component 23. The saline solution enters the hollow cavity 112 of the microdialysis probe 11 through the inlet tube 121. Since the outermost layer of the microdialysis probe 11 is a semi-permeable membrane 111, small molecule glycosides in the sample can pass through the semi-permeable membrane 111 into the hollow cavity 112 under the action of concentration difference and fluid flow, and flow out through the outlet tube 122 along with the saline solution. They then enter the collection component 22 in the execution housing 21 for enrichment and temporary storage. After the collection component 22 completes the sample collection for a preset time, the flushing part of the external detection device is connected through the detection interface 211. The flushing liquid is used to flush the glycoside sample enriched in the collection component 22 into the detection part of the detection device, realizing continuous sampling and subsequent detection of glycoside samples.

[0037] Reference Figure 3 and Figure 4The collection assembly 22 includes a collection tank 221 and an extraction pump 222. One end of the extraction pump 222 is connected to the top of the collection tank 221, and the other end is connected to the outlet pipe 122. Porous graphitized carbon 223 is disposed inside the collection tank 221. An inlet 2211 is provided on one side of the upper part of the collection tank 221, which is connected to a detection interface 211. A detection port 2212 is provided at the bottom of the collection tank 221, penetrating the execution housing 21 and located outside the execution housing 21. A drain port 2213 is provided on one side of the lower part of the collection tank 221, penetrating the execution housing 21. The collection assembly 22... During operation, pump 222 draws physiological saline carrying the glycosides to be tested from outlet pipe 122 into collection tank 221. Porous graphitized carbon 223 in collection tank 221 adsorbs and enriches the glycosides. The inlet 2211 on the upper side of collection tank 221 is connected to the detection interface 211 for flushing the external detection device. After the preset collection and enrichment time is completed, the flushing liquid flows into collection tank 221 through inlet 2211, elutes the glycosides sample adsorbed on porous graphitized carbon 223, and discharges it to the external detection device through detection port 2212 at the bottom of collection tank 221. Drain port 2213 on the lower side of collection tank 221 is used to discharge the waste liquid generated during the collection process.

[0038] Reference Figure 3 and Figure 4A switching valve 3 is installed on the top of the collection tank 221. The switching valve 3 includes a ball valve 31, a valve shaft 32, a valve stem 33, and a torsion spring 34. The ball valve 31 is rotatably installed inside the collection tank 221. A through hole 311 is provided on the ball valve 31, and a detection inlet 312 is provided on one side of the ball valve 31, communicating with the through hole 311. One end of the valve shaft 32 is fixedly installed on the ball valve 31, and the other end of the valve shaft 32 is located outside the collection tank 221, rotatably connected to the collection tank 221. One end of the valve stem 33 is fixedly installed on the other end of the valve shaft 32. The torsion spring 34 is sleeved on the other end of the valve shaft 32, one end of the torsion spring 34 is fixedly installed on the collection tank 221, and the other end of the torsion spring 34 is fixedly installed on the valve stem 33. A torsion spring 34 is provided on one side of the valve stem 33. A limiting groove 331 is provided. In the sample enrichment state, the ball valve 31 is kept in its initial position under the action of the torsion spring 34. The through hole 311 is arranged longitudinally, and the physiological saline carrying the glycoside analyte can flow smoothly through the through hole 311 into the porous graphitized carbon 223 in the collection tank 221 to achieve the adsorption and enrichment of the analyte. When the preset enrichment time is reached, the external force drives the valve stem 33 to drive the valve shaft 32 and the ball valve 31 to rotate under the force of the torsion spring 34, so that the detection inlet 312 on the ball valve 31 is connected to the inlet 2211 at the top of the collection tank 221. At this time, the external flushing liquid can enter the collection tank 221 through the inlet 2211, the detection inlet 312 and the through hole 311 to elute the glycoside sample adsorbed on the porous graphitized carbon 223, and complete the automatic switching between enrichment and elution states.

[0039] Reference Figure 3 and Figure 4A switching assembly 4 is provided at the bottom of the collection tank 221. The switching assembly 4 includes a switching ball 41, a switching shaft 42, a switching rod 43, and a connecting rod 44. The switching ball 41 is rotatably disposed inside the collection tank 221. A switching hole 411 is provided through the switching ball 41. A waste liquid discharge hole 412 is provided on one side of the switching ball 41, and the waste liquid discharge hole 412 communicates with the switching hole 411. One end of the switching shaft 42 is fixedly disposed on the switching ball 41, and the other end of the switching shaft 42 is disposed outside the collection tank 221. The switching shaft 42 is rotatably connected to the collection tank 221. One end of the switching rod 43 is fixedly disposed on the other end of the switching shaft 42. The connecting rod 44... One end of the switching rod 43 is rotatably mounted on the other end of the switching rod 44, and the other end of the connecting rod 44 is rotatably mounted on the other end of the valve stem 33. The switching assembly 4 at the bottom of the collection tank 221 is linked with the top switching valve 3 through the connecting rod 44. In the enrichment state, the waste liquid discharge hole 412 of the switching ball 41 is connected to the drain outlet 2213, which can discharge the waste liquid during the collection process. When the switching valve 3 rotates to the detection state under the action of the torsion spring 34, the valve stem 33 drives the switching rod 43, the switching shaft 42 and the switching ball 41 to rotate synchronously through the connecting rod 44, so that the switching hole 411 of the switching ball 41 is open, and the enriched sugar sample can enter the detection device through the switching hole 411 to complete the sample delivery for testing.

[0040] Reference Figure 3 and Figure 5 The input component 23 includes an input tube 231, a rotating shaft 232, a turbine 233, and a push pump 235. One end of the rotating shaft 232 is located inside the input tube 231, and the other end is located outside the input tube 231. The rotating shaft 232 is rotatably connected to one end of the input tube 231. One end of the push pump 235 is connected to one end of the input tube 231, and the other end is connected to the inlet pipe 121. The other end of the input tube 231 is connected to the infusion unit 7. The turbine 233 is located inside the input tube 231 and is fixedly mounted on one end of the rotating shaft 232. The rotating shaft 232 is located outside the input tube 231. The shaft 232 is threaded; a spiral spring 234 is sleeved on the other end of the rotating shaft 232. One end of the outer ring of the spiral spring 234 is fixedly mounted on the input tube 231, and one end of the inner ring of the spiral spring 234 is fixedly mounted on the rotating shaft 232. When the input component 23 is working, the push pump 235 provides power to drive the turbine 233 to rotate by delivering the saline solution from the infusion unit 7 to the input tube 231. The turbine 233 drives the rotating shaft 232 to rotate synchronously, and at the same time, it tightens the spiral spring 234 sleeved on the rotating shaft 232. When the push pump 235 stops working, the spiral spring 234 releases its elastic force, driving the rotating shaft 232 to rotate in the opposite direction and reset.

[0041] Reference Figure 3 and Figure 5A drive assembly 5 is provided inside the execution housing 21. The drive assembly 5 includes a guide rail 51, a slider 52, a drive sleeve 53, a drive spring 54, and a drive rod 55. The guide rail 51 is located on one side of the rotating shaft 232 and is fixedly installed on the inner wall of the execution housing 21. The slider 52 is slidably mounted on the guide rail 51, and the middle part of the slider 52 is threaded onto the rotating shaft 232. One end of the drive sleeve 53 is fixedly mounted on the slider 52, and the drive rod 55 is slidably mounted inside the drive sleeve 53. The drive spring 54 is installed inside the drive sleeve 53, with one end of the drive spring 54 fixedly mounted on the drive sleeve 53 and the other end of the drive spring 54 fixedly mounted on the drive sleeve 53. The drive rod 55 is fixed at one end; the other end of the drive rod 55 abuts against the switching valve 3; when the drive assembly 5 is working, the rotating shaft 232 rotates, driving the slider 52, which is slidably mounted on the guide rail 51, to move through the threaded transmission; the drive sleeve 53 moves synchronously with the slider 52, and the drive spring 54 inside the drive sleeve 53 applies elastic force, so that the drive rod 55 always presses against the valve stem 33 of the switching valve 3, thereby ensuring that the valve stem 33 is in a fixed position in the early stage and does not rotate; when the slider 52 moves to the top of the guide rail 51, the torque on the valve stem 33 tends to zero, the torsion spring 34 releases the restoring force, and drives the valve stem 33 to rotate, realizing the state switching of the switching valve 3.

[0042] Reference Figure 1 A waste liquid unit 6 is installed on the drain outlet 2213. The waste liquid unit 6 includes a second pipe 61 and a waste liquid bag 62. One end of the second pipe 61 is connected to the drain outlet 2213. The waste liquid bag 62 is connected to the other end of the second pipe 61. The infusion unit 7 includes a third pipe 71 and an infusion bag 72. One end of the third pipe 71 is connected to the other end of the input pipe 231. The infusion bag 72 is connected to the other end of the third pipe 71. When the waste liquid unit 6 is working, the waste liquid inside the collection tank 221 enters the second pipe 61 through the drain outlet 2213, and then flows into the waste liquid bag 62 through the second pipe 61 for centralized collection, realizing unified storage and treatment of waste liquid. When the infusion unit 7 is working, the saline in the infusion bag 72 is transported to the input pipe 231 through the third pipe 71 to continuously provide the dialysis liquid required by the device, ensuring that the sampling process is stable and continuous.

[0043] The working principle of the continuous sampling device for glucose group detection in this application is as follows: the infusion unit 7 provides the fluid required for dialysis, and the saline in the infusion bag 72 is transported to the input tube 231 of the input component 23 through the third pipe 71; when the input component 23 is working, the push pump 235 drives the saline to flow, driving the turbine 233 and the rotating shaft 232 to rotate, which on the one hand causes the spiral spring 234 to be wound and stored, and on the other hand drives the slider 52 to move upward along the guide rail 51 through the threaded transmission, and the slider 52 drives the drive sleeve 53 to move synchronously; in the enrichment state, the through hole 311 on the ball valve 31 is arranged longitudinally, and the switching component 4 at the bottom of the collection tank 221 is linked by the connecting rod 44 to switch the ball 41. Waste liquid drain hole 412 is connected to drain outlet 2213, and waste liquid unit 6 is in waste liquid collection state; push pump 235 continuously delivers physiological saline to inlet pipe 121 and enters the hollow cavity 112 of microdialysis probe 11. The probe tip is inserted into the sample to be tested. Under the action of semipermeable membrane 111 and concentration difference, small molecule glycosides in the sample pass through semipermeable membrane 111 into the cavity and mix with physiological saline; extraction pump 222 draws physiological saline carrying glycosides into collection tank 221, where porous graphitized carbon 223 adsorbs and enriches the glycosides, realizing sample capture and concentration; waste liquid generated during enrichment enters waste liquid bag 62 for centralized collection through drain outlet 2213 and second pipe 61; as rotation Shaft 232 rotates continuously, and slider 52 moves upward continuously. When slider 52 moves to the top of guide rail 51, the torque of drive rod 55 on valve rod 33 approaches zero. Torsion spring 34 drives valve rod 33, valve shaft 32, and ball valve 31 to rotate, switching valve 3 to detection state, connecting detection inlet 312 with inlet 2211 at the top of collection tank 221. While valve rod 33 rotates, it drives switching assembly 4 to move synchronously through connecting rod 44, making switching hole 411 of switching ball 41 open, disconnecting waste liquid discharge hole 412 from drain outlet 2213. External flushing liquid enters collection tank 221 through detection interface 211, inlet 2211, detection inlet 312, and ball valve 31 through hole 311, filling the porous... The glycoside sample adsorbed on graphitized carbon 223 is eluted. The eluted sample enters the external detection device through the switching hole 411 and the detection port 2212 to complete the glycoside sample detection. After the detection is completed, the push pump 235 stops working, the spiral spring 234 releases its elastic force to drive the rotating shaft 232 to reverse and reset. The slider 52, the drive sleeve 53 and the drive rod 55 return to their original positions synchronously. The drive rod 55 presses against the valve rod 33 again to reset it. The switching valve 3 and the switching component 4 return to the initial enrichment state under the linkage of the torsion spring 34 and the connecting rod 44. The infusion unit 7 and the waste liquid unit 6 continue to work. The device returns to the initial state and can carry out the next round of continuous sampling and detection, thereby realizing continuous, stable and automated sampling and detection of glycoside samples.

[0044] The above are all preferred embodiments of this application, and are not intended to limit the scope of protection of this application. Therefore, all equivalent changes made in accordance with the structure, shape and principle of this application should be covered within the scope of protection of this application.

Claims

1. A continuous sampling device for glycoside detection, characterized in that: It includes a sampling unit (1) and an execution unit (2); the sampling unit (1) includes a microdialysis probe (11) and a first conduit (12), one end of the microdialysis probe (11) is disposed on the execution unit (2) through the first conduit (12); the other end of the microdialysis probe (11) is a tip, and the outermost layer of the microdialysis probe (11) is a semi-permeable membrane (111); the interior of the microdialysis probe (11) is a hollow cavity (112); the execution unit (2) includes an execution housing ( 21) The execution housing (21) is provided with a trapping component (22) and an input component (23) inside. The first pipeline (12) is connected to the trapping component (22) and the input component (23) respectively. A detection interface (211) is provided on one side of the execution housing (21), and the detection interface (211) is connected to the trapping component (22). An infusion unit (7) is provided on one side of the execution housing (21), and the infusion unit (7) is connected to the input component (23).

2. The continuous sampling device for glycoside detection according to claim 1, characterized in that: The first conduit (12) is provided with an inlet pipe (121) and an outlet pipe (122). One end of the inlet pipe (121) and one end of the outlet pipe (122) are located in the hollow cavity (112) of the microdialysis probe (11). The inlet pipe (121) is located in the execution housing (21) through the first conduit (12), and the other end of the inlet pipe (121) is connected to the input component (23). The outlet pipe (122) is located in the execution housing (21) through the first conduit (12), and the other end of the outlet pipe (122) is connected to the collection component (22).

3. The continuous sampling device for glycoside detection according to claim 2, characterized in that: The collection assembly (22) includes a collection tank (221) and an extraction pump (222). One end of the extraction pump (222) is connected to the top of the collection tank (221), and the other end of the extraction pump (222) is connected to the outlet pipe (122). Porous graphitized carbon (223) is provided inside the collection tank (221). An inlet (2211) is provided on one side of the upper part of the collection tank (221), and the inlet (2211) is connected to the detection interface (211). A detection port (2212) is provided at the bottom of the collection tank (221), and the detection port (2212) penetrates the execution housing (21) and is located outside the execution housing (21). A drain port (2213) is provided on one side of the lower part of the collection tank (221), and the drain port (2213) penetrates the execution housing (21).

4. The continuous sampling device for glycoside detection according to claim 2, characterized in that: The input component (23) includes an input tube (231), a rotating shaft (232), a turbine (233), and a push pump (235); one end of the rotating shaft (232) is located inside the input tube (231), and the other end of the rotating shaft (232) is located outside the input tube (231), and the rotating shaft (232) is rotatably connected to one end of the input tube (231); one end of the push pump (235) is connected to one end of the input tube (231), and the other end of the push pump (235) is connected to the inlet pipe (121); the other end of the input tube (231) is connected to the infusion unit (7); the turbine (233) is located inside the input tube (231), and the turbine (233) is fixedly installed at one end of the rotating shaft (232); the rotating shaft (232) located outside the input tube (231) is provided with threads.

5. The continuous sampling device for glycoside detection according to claim 3, characterized in that: A switching valve (3) is provided on the top of the collection tank (221); the switching valve (3) includes a ball valve (31), a valve shaft (32), a valve stem (33), and a torsion spring (34). The ball valve (31) is rotatably disposed inside the collection tank (221). A through hole (311) is provided on the ball valve (31). A detection inlet (312) is provided on one side of the ball valve (31), and the detection inlet (312) communicates with the through hole (311). One end of the valve shaft (32) is fixedly disposed on the ball valve. On the valve (31), the other end of the valve shaft (32) is located outside the collection tank (221), and the valve shaft (32) is rotatably connected to the collection tank (221); one end of the valve stem (33) is fixedly located on the other end of the valve shaft (32); the torsion spring (34) is sleeved on the other end of the valve shaft (32), one end of the torsion spring (34) is fixedly located on the collection tank (221), and the other end of the torsion spring (34) is fixedly located on the valve stem (33); a limit groove (331) is provided on one side of the valve stem (33).

6. The continuous sampling device for glycoside detection according to claim 5, characterized in that: A switching assembly (4) is provided at the bottom of the collection tank (221); the switching assembly (4) includes a switching ball (41), a switching shaft (42), a switching rod (43), and a connecting rod (44). The switching ball (41) is rotatably disposed inside the collection tank (221). A switching hole (411) is provided through the switching ball (41). A waste liquid discharge hole (412) is provided on one side of the switching ball (41). The waste liquid discharge hole (412) and the switching hole (411) are connected. The switching shaft (42) is fixedly mounted on the switching ball (41) at one end, and the other end of the switching shaft (42) is mounted outside the collection tank (221). The switching shaft (42) is rotatably connected to the collection tank (221). One end of the switching rod (43) is fixedly mounted on the other end of the switching shaft (42). One end of the connecting rod (44) is rotatably mounted on the other end of the switching rod (43), and the other end of the connecting rod (44) is rotatably mounted on the other end of the valve stem (33).

7. The continuous sampling device for glycoside detection according to claim 4, characterized in that: The actuator housing (21) is provided with a drive assembly (5), which includes a guide rail (51), a slider (52), a drive sleeve (53), a drive spring (54), and a drive rod (55). The guide rail (51) is located on one side of the rotating shaft (232) and is fixedly installed on the inner wall of the actuator housing (21). The slider (52) is slidably installed on the guide rail (51), and the middle part of the slider (52) is threaded onto the rotating shaft (232). One end of the drive sleeve (53) is fixedly installed on the slider (52), and the drive rod (55) is slidably installed inside the drive sleeve (53). The drive spring (54) is installed inside the drive sleeve (53), one end of the drive spring (54) is fixedly installed on the drive sleeve (53), and the other end of the drive spring (54) is fixedly installed on one end of the drive rod (55). The other end of the drive rod (55) abuts against the switching valve (3).

8. The continuous sampling device for glycoside detection according to claim 4, characterized in that: A spiral spring (234) is sleeved on the other end of the rotating shaft (232). One end of the outer ring of the spiral spring (234) is fixedly mounted on the input tube (231), and one end of the inner ring of the spiral spring (234) is fixedly mounted on the rotating shaft (232).

9. A continuous sampling device for glycoside detection according to claim 3, characterized in that: A waste liquid unit (6) is provided on the drain outlet (2213). The waste liquid unit (6) includes a second pipe (61) and a waste liquid bag (62). One end of the second pipe (61) is connected to the drain outlet (2213). The waste liquid bag (62) is connected to the other end of the second pipe (61).

10. A continuous sampling device for glycoside detection according to claim 4, characterized in that: The infusion unit (7) includes a third pipe (71) and an infusion bag (72). One end of the third pipe (71) is connected to the other end of the input tube (231); the infusion bag (72) is connected to the other end of the third pipe (71).

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