Optical detection device

Abstract

The application provides an optical detection device, comprising: a main body support module comprising a fixedly connected frame and a mounting plate; a stage module comprising a moving platform and a slide processing device integrated in the moving platform, the moving platform having a bearing surface facing the mounting plate, the bearing surface being used for bearing a biological slide, the moving platform further having a mounting position for accommodating a reagent box, the reagent box being used for containing a reaction reagent, and the slide processing device being used for loading the reaction reagent onto the biological slide; a detection light path module mounted on the mounting plate and used for emitting excitation light to irradiate a biological sample on the biological slide and collecting a light signal generated by the biological sample under irradiation of the excitation light; the moving platform is movably arranged to synchronously drive the slide processing device and the reagent box to translate; the detection light path module comprises an objective lens movably arranged along an optical axis direction, and the light signal is collected through the objective lens; and the excitation light is projected onto different positions of the biological slide to scan the biological sample as the moving platform translates.

Description

Technical Field

[0001] This application relates to the field of optical inspection technology, and in particular to an optical inspection device. Background Technology

[0002] Gene sequencers are highly complex technological integrations involving multiple disciplines and fields, including optics, mechanics, electronics, fluid dynamics, software, and algorithms. Mechanically, gene sequencers primarily involve multiple hardware modules such as fluid dynamics, biochemistry, temperature control, electronics, motion control platforms, fluorescence detection systems, and computers.

[0003] The aforementioned gene sequencer is a complex device with numerous, independently distributed components, making it exceptionally large and posing significant inconveniences during transportation, installation, and operation. Furthermore, the gene sequencer comprises multiple functional modules, each requiring perfect coordination and sufficient stability to achieve optimal performance from the high-throughput fluorescence detection system, thereby ensuring the accuracy and reliability of the sequencing results. Summary of the Invention

[0004] This application provides an optical detection device, comprising: a main support module including a fixedly connected frame and a mounting plate, the frame and mounting plate enclosing a detection space; a stage module located within the detection space, including a moving platform and a slide processing device integrated in the moving platform, the moving platform having a bearing surface facing the mounting plate for bearing biological slides, the moving platform also having a mounting position for accommodating reagent kits, the reagent kits being used to hold reaction reagents, the slide processing device being used to load the reaction reagents onto the biological slides; and a detection optical path module. A module, mounted on the mounting plate, is used to emit excitation light to irradiate the biological sample on the biological slide and to collect the light signal generated by the biological sample under the excitation light irradiation; wherein, the moving platform is movable within the moving space along the optical axis perpendicular to the detection optical path module to synchronously drive the slide processing device and the reagent kit to translate; the detection optical path module includes an objective lens movable along the optical axis, and the light signal is collected by the objective lens; the excitation light is projected onto different positions on the biological slide as the moving platform translates to scan the biological sample.

[0005] The aforementioned optical detection device includes a stage module, which comprises a movable platform with mounting positions and a slide processing device integrated into the movable platform. The mounting positions are used to accommodate reagent kits, which are used to load biological reagents. This allows the movable platform to synchronously move the reagent kits and slide processing device loaded with biological reagents during the detection process. Thus, there is no need for external reagent supply components for the optical detection device. Therefore, the overall integration of the aforementioned optical detection device is high, which helps to reduce the overall size of the device and facilitates transportation, installation, and optical detection operations. Furthermore, the integration of the reagent kits and slide processing device into the movable platform eliminates the need for additional installation space, thereby improving space utilization. Attached Figure Description

[0006] Figure 1 This is a three-dimensional structural diagram of an optical inspection device according to an embodiment of this application.

[0007] Figure 2 This is another three-dimensional structural diagram of the optical inspection device according to an embodiment of this application.

[0008] Figure 3 for Figure 1 A three-dimensional structural diagram of the main support module and vibration damping module.

[0009] Figure 4 for Figure 3 Exploded view of the main support module and vibration damping module.

[0010] Figure 5 for Figure 4 A three-dimensional structural diagram of the mounting plate.

[0011] Figure 6 for Figure 1 A partial three-dimensional structural diagram of the detection optical path module.

[0012] Figure 7 for Figure 1 Middle objective adjustment assembly and Figure 6 A three-dimensional structural diagram of the objective lens.

[0013] Figure 8 for Figure 1 Middle objective adjustment assembly and Figure 6 Another three-dimensional structural diagram of the objective lens.

[0014] Figure 9 for Figure 7 A schematic diagram of the cross-sectional structure along line AA.

[0015] Figure 10 for Figure 6 A three-dimensional structural diagram of a mid-wave splitter.

[0016] Figure 11for Figure 6 Another three-dimensional structural diagram of the mid-splitter assembly.

[0017] Figure 12 for Figure 6 Exploded view of the mid-splitter module.

[0018] Figure 13 for Figure 12 Exploded view of the mid-spectrum adjustment component and the third color separator.

[0019] Figure 14 for Figure 1 Medium tube lens adjustment assembly and Figure 6 A three-dimensional structural diagram of a medium-diameter telescope.

[0020] Figure 15 for Figure 14 A three-dimensional structural diagram of the adjustment assembly for the middle tube mirror.

[0021] Figure 16 for Figure 1 Central camera adjustment components and Figure 6 A schematic diagram of the three-dimensional structure of a camera.

[0022] Figure 17 for Figure 16 Exploded view of the camera adjustment components and the camera.

[0023] Figure 18 for Figure 16 A schematic diagram of another three-dimensional structure of the camera adjustment components and the camera.

[0024] Explanation of main component symbols Optical inspection equipment: 100; Laser opening: 4231; Main support module: 10; First fluorescence opening: 4232; Inspection space: 11; First mounting window: 4241; Frame: 12; First light-shielding and dustproof cover: 4242; First side: 121; Second mounting window: 4251; Mounting holes: 1211, 1231; Second light-shielding and dustproof cover: 4252; Bottom: 122; Second fluorescence opening: 4261; Moving plane: 122; Laser reference support: 4271; Second side: 123; Focusing reference support: 4272; Mounting plate: 13; Fluorescent reference surface: 4273; Mounting plane: 131; Circular pin holes: 4281, 442; Reference mounting surface: 132; Groove Pin holes: 4282, 443; Mounting positioning holes: 133; Screws: 4291, 4292; Clearance notch: 134; Spectrometer adjustment assembly: 43; Reinforcing rib: 135; Fixing frame: 431; Recess: 136; Frame structure: 4311; Stage module: 20; Connecting block: 4312; Moving platform: 21; Color separator mounting position: 4313; Bearing surface: 211; Adapter block: 432; Mounting position: 212; Screws: 4321, 4322; Opening: 2121; Adjustment seat: 433; Slide handling device: 22; Screws: 4331; Detection optical path module: 30; Set screw holes: 4332, 4516; Light source: 31; Tube lens adjustment assembly: 44; Objective lens: 32; Support clamping Mechanism: 441; Lens tube: 33; Finished surface: 444; Camera: 34; Camera adjustment assembly: 37; Autofocus assembly: 35; Mounting base plate: 371; Light guide assembly: 36; First surface: 3711; First dichroic filter: 361; Second surface: 3712; Second dichroic filter: 362; Slots: 3713, 3723, 3746; Third dichroic filter: 363; Side plate: 3715; Fluorescence acquisition channels: 301, 302; First guide groove: 3717; Optical path mounting and adjustment module: 40; Groove: 3718; Objective lens adjustment assembly: 41; Camera bracket: 372; Objective lens holder: 411; Base plate: 3721; Locking part: 4111; Stand plate: 3722; Side edge: 411 2; Camera fixing ring: 373; Objective lens adapter: 412; Up / down adjustment component: 374; Mounting opening: 4121; Adjustment plate: 3741; Wedge-shaped adjustment ring: 413; Second fixing block: 3742; Ring part: 4131; Up / down adjustment screw: 3744; Wedge part: 4132; Second guide groove: 3747; Top surface: 4133; Front / back adjustment component: 375; Screws: 414, 445, 4514, 4524, 4543, 4545, 4552, 4562, 4566; First fixing block: 3751; Support platform: 415; Front / back adjustment screw: 3753; Motor: 416; Rotation adjustment component: 376; Reference support: 42; Fixing hole: 3761; Base plate: 421;Pin holes: 3763, 3767; Objective lens opening: 4211; Flange: 3764; Finished stepped surface: 4212, 4213; Locking hole: 3765; Top plate: 422; Vibration damping module: 50; Focusing opening: 4221; Base plate: 51; Mounting slot: 4222; Support column: 52; Side plates: 423, 424, 425, 426; Vibration damping assembly: 521.

[0025] The following detailed description, in conjunction with the accompanying drawings, will further illustrate this application. Detailed Implementation

[0026] This application provides an optical detection device, including a stage module capable of synchronously moving biological slides. The stage module includes a moving platform and a slide processing device integrated into the moving platform. The moving platform also forms a mounting position for accommodating reagent kits, which is beneficial for improving the overall integration of the optical detection device and for miniaturizing the optical detection device.

[0027] Please refer to the following: Figure 1 and Figure 2 The optical inspection device 100 of this embodiment includes a main support assembly 10, a stage module 20, and a detection optical path module 30. The main support assembly 10 forms a detection space 11. The stage module 20 is located within the detection space 11 and is movable within the detection space 11. The detection optical path module 30 is connected to the main support assembly 10. When the optical inspection device 100 is placed on a work platform (e.g., a desktop or other horizontal operating surface), the detection optical path module 30 is located above and spaced apart from the stage module 20. The main support assembly 10 is used to support and install various functional modules (e.g., the stage module 20 and the detection optical path module 30) within the optical inspection device 100. The stage module 20 is used to carry a biological slide. The detection optical path module 30 is used to detect information about the biological sample on the biological slide.

[0028] The main support component 10 includes a frame 12 that is fixedly connected to each other and a mounting plate 13 that is mounted on the frame 12. The frame 12 and the mounting plate 13 enclose a detection space 11.

[0029] Please see Figure 3 In this embodiment, the frame 12 has a first side 121, a bottom 122, and a second side 122 connected in sequence. The bottom 122 is a plate-like structure and has a generally rectangular moving plane 1221. The first side 121 and the second side 122 are respectively connected to two opposite edges of the moving plane 1221, making the frame 12 as a whole "U" shaped. In this embodiment, the first side 121, the bottom 122, and the second side 122 are all castings with reinforcing ribs, or they can be machined parts that have been assembled. The moving plane of the bottom 122 is a high-precision, high-flatness surface.

[0030] In this embodiment, the two opposite edges of the mounting plate 13 are respectively fixedly mounted on the ends of the first side 121 and the second side 122 away from the bottom 122. The mounting plate 13 is also a plate-shaped structure, forming a mounting plane 131 that is opposite to the bottom 122 and parallel to the moving plane 1221. Please refer to the following: Figure 3 and Figure 4 The optical inspection device 100 is connected to the mounting plane 131. In this embodiment, the first side 121 and the second side 122 are respectively provided with four mounting holes 1211 and 1231 at one end of the mounting plate 13 for fixing the mounting plate 13.

[0031] Please refer to the following: Figure 3 In this embodiment, the mounting plate 13 also includes multiple precision-machined planes located on the mounting plane 131, serving as reference mounting surfaces 132. Each reference mounting surface 132 is provided with at least one mounting positioning hole 133. The detection optical path module 30 includes multiple functional units, and each reference mounting surface 132 is used to mount and position one of the functional units to accurately determine the position of each optical component in the functional unit in the entire optical path. The mounting plate 13 has at least one clearance notch 134 on each of the opposite sides of the unconnected first side 121 and second side 122 to facilitate the installation, disassembly, wiring, and maintenance of the various structures integrated in the stage module 20. The mounting plate 13 also includes two 135s disposed on the mounting plane 131. The two reinforcing ribs are both elongated and have different lengths to strengthen the rigid support of the mounting plate 13, ensuring that the deformation and vibration modes of the mounting plate 13 meet the equipment requirements.

[0032] Please see Figure 5 The lower surface of the mounting plate 13 (the surface facing the platform module 20) has a honeycomb-like structure, that is, it has multiple recesses 136 arranged at intervals. In this way, on the one hand, it helps to reduce the transmission of external vibrations and reduce rigid deformation, thereby avoiding the adverse effects of vibration and deformation caused by external factors or the movement of the platform module 20 on the performance of the equipment; on the other hand, it reduces the weight of the entire equipment while ensuring the rigid support of the structure.

[0033] Please refer to this again. Figure 1 and Figure 2The stage module 20 is movably connected to the moving plane 1221. The stage module 20 includes a moving platform 21 and a slide processing device 22 integrated into the moving platform 21. The moving platform 21 is movably connected to the moving plane 1221. The moving platform 21 has a bearing surface 211 facing away from the bottom 122. The bearing surface 211 is used to bear biological slides. In this embodiment, the moving platform 21 forms two reagent kit mounting positions 212, which are symmetrically distributed. Each reagent kit mounting position 212 is a rectangular space for accommodating reagent kits, which are used to hold reaction reagents. In this embodiment, the opening 2121 of each reagent kit mounting position 212 is formed in the side plate of the moving platform 21 (perpendicular to the moving plane 1221) and faces the direction where the first side 121 and the second side 123 are not provided, to facilitate the placement and removal of reagent kits. The slide processing device 22 is used to load the reaction reagents from the reagent kit onto the biological slide for optical detection.

[0034] The two opposite edges of the moving plane 1221 that are not connected to the first side 121 and the second side 123 extend along the X direction, and the two opposite edges of the moving plane 1221 that are connected to the first side 121 and the second side 123 extend along the Y direction, with the X direction perpendicular to the Y direction. The moving platform 21 can translate along the X and Y directions within the detection space 11 (i.e., on the moving plane 1221). When the bearing surface 211 carries the biological slide and the reagent kit mounting position 212 contains the reagent kit, the moving platform 21 can drive the biological slide, the reagent kit, and the slide processing device to translate synchronously.

[0035] In this embodiment, the mobile platform 21 uses a high-speed linear motor (not shown) to achieve high-speed movement in the X and Y directions. The control precision of the motor speed and the positioning precision during the movement can both reach the nanometer level.

[0036] The detection optical path module 30 is used to emit laser light as excitation light toward the stage module 20. The biological slide on the stage module 20 carries a biological sample. The biomarkers in the biological sample carry fluorescent groups. The fluorescent groups are excited and produce fluorescence under the excitation light. The detection optical path module 30 is also used to collect the fluorescence to obtain information about the biological sample (biomarker content, biomarker distribution pattern, etc.).

[0037] Please see Figure 6The detection optical path module 30 includes a light source 31, an objective lens 32, a telescope lens 33, a camera 34, an autofocus assembly 35, and a beam splitter 36. The light source 31 emits the excitation light. The autofocus assembly 35 emits focusing light during operation to focus the objective lens 32. The objective lens 32 focuses the excitation and focusing light onto the biological slide and collects the light signals reflected from the biological sample (including fluorescence and focusing signals; the fluorescence signal is the aforementioned laser beam). The telescope lens 33 focuses the laser beam onto the photosensitive surface of the camera 34.

[0038] Beam-splitter 36 is located in the optical path of the excitation light and the laser beam, and includes a first beam splitter 361, a second beam splitter 362, and a third beam splitter 363. The light-receiving surface of the first beam splitter 361 is set at 45° to the optical axes of the excitation light and the laser beam, respectively, for reflecting the excitation light from the light source 31 to the objective lens 32, and for transmitting the laser beam from the objective lens 32 to the second beam splitter 362. The second beam splitter 362 is set at 45° to the optical axis of the laser beam, for reflecting the laser beam to the third beam splitter 363. During autofocus, the second beam splitter 362 is also used to transmit the focusing beam.

[0039] In this embodiment, the biological slide reflects multiple (e.g., two or four) different colors of laser light corresponding to various markers. The detection optical path module 30 includes two tube lenses 33 and two cameras 34. One tube lens 33 and one camera 34 form a first fluorescence acquisition channel 301, and the other tube lens 33 and the other camera 34 form a second fluorescence acquisition channel 302. The first fluorescence acquisition channel 301 and the second fluorescence acquisition channel 302 constitute the fluorescence detection module of the optical detection device 100. The optical axes of the first fluorescence acquisition channel 301 and the second fluorescence acquisition channel 302 are perpendicular to each other and parallel to the mounting plate 13. The first fluorescence acquisition channel 301 and the second fluorescence acquisition channel 302 are respectively used to collect and sense laser light of different wavelengths. The third beam splitter 363 is used to selectively reflect the received laser light to the first fluorescence acquisition channel 301 or transmit it to the second fluorescence acquisition channel 302 according to the wavelength of the received laser light.

[0040] In this embodiment, to achieve high-precision detection, the positions and angles of each optical element (including at least the objective lens 32, the tube lens 33, the camera 34, etc.) in the detection optical path module 30 are subject to high requirements. Therefore, please refer to [further details needed]. Figure 1 The optical inspection device 100 in this embodiment also includes an optical path installation and adjustment module 40, which is used to install, position and adjust the position and angle of each optical element in the detection optical path module 30.

[0041] Please refer to the following: Figures 7-9The optical path mounting and adjustment module 40 includes an objective lens adjustment assembly 41, which is used to connect the objective lens 32 to the mounting plate 13 and to realize the eccentricity adjustment, tilt angle adjustment and height adjustment of the objective lens 31.

[0042] In this embodiment, the objective lens adjustment assembly 41 includes an objective lens holder 411, an objective lens adapter 412, a wedge-shaped adjustment ring 413, a screw 414, a support platform 415, and a motor 416. The objective lens holder 411 includes an integrally formed engaging portion 4111 and a side edge 4112. The engaging portion 4111 is a cylindrical structure and is fitted onto one end of the objective lens 33, that is, one end of the objective lens 33 is engaged and fixed within the engaging portion 4111. The side edge 4112 extends outward from one end of the engaging portion 4111 (in a circumferential direction away from the objective lens 33). The objective lens adapter 412 has a mounting opening 4121. The objective lens 33 and the engaging portion 4111 are located within the mounting opening 4121, and the side edge 4111 is located below the objective lens adapter 412 (on the side away from the mounting plate 13). The wedge-shaped adjustment ring 413 includes an integrally formed ring portion 4131 and a wedge-shaped portion 4132. The ring portion 4131 surrounds the periphery of the side edge 4111. The wedge-shaped portion 4132 extends inward (towards the objective lens 33) from one end of the ring portion 4131 near the objective lens adapter 412. The wedge-shaped portion 4132 surrounds the periphery of the engaging portion 4111 and extends between the side edge 4111 and the objective lens adapter 412.

[0043] In this embodiment, the objective lens adjustment assembly 41 includes three screws 414. The three screws 414 are arranged at equal intervals around the periphery of the objective lens 33. Each screw 414 passes through the side edge 4111, the wedge-shaped portion 4132 and the objective lens adapter 412 in sequence to lock the objective lens retainer 411, the objective lens adapter 412 and the wedge-shaped adjustment ring 413.

[0044] In this embodiment, the wedge-shaped portion 4132 has a top surface 4133 facing the objective lens adapter 412, and the top surface 4133 is a wedge-shaped surface. That is, the thickness of the wedge-shaped portion 4132 is uneven and gradually changes along the optical axis of the objective lens 33. When the screw 414 is tightened, the top surface 4133 of the wedge-shaped portion 4132 is in close contact with the objective lens adapter 412. When the screw 414 is loosened, the distance between the top surface 4133 of the wedge-shaped portion 4132 and the objective lens adapter 412 increases relatively. Therefore, in this embodiment, by adjusting (tightening or loosening) each screw 414, the distance between the top surface 4133 and the objective lens adapter 412 can be adjusted, thereby adjusting the tilt angle of the top surface 4133, that is, adjusting the angle between the top surface 4133 and the surface of the biological slide that carries the biological sample. Since the objective lens 33 is fixed to the objective lens adapter 412, and the objective lens adapter 412 is fixed to the wedge-shaped adjustment ring 413 by screws 414, the aforementioned adjustment of the screws 414 can simultaneously cause the objective lens 33 to tilt. Furthermore, by selecting and adjusting one, two, or three screws 414, and controlling the tightness of each screw 414, the tilt of the objective lens 33 in different directions can be controlled. Therefore, the wedge-shaped adjustment ring 413, the adjustment screws 414, and the objective lens adapter 412 can achieve fine adjustment of the perpendicularity of the objective lens 33 relative to the object plane and the image plane; with the help of software, the perpendicularity accuracy can reach the second level.

[0045] In this embodiment, the objective lens holder 411 and the objective lens adapter 412 are provided with an appropriate amount of translation adjustment, which can realize the translation adjustment of the objective lens 33 (translation on a plane perpendicular to the optical axis) to achieve the eccentric adjustment of the objective lens 33 relative to the optical axis of the entire system. With the help of the three-point locking screw 414, after the adjustment is completed, the state of the entire objective lens 33 has extremely high stability and reliability.

[0046] In this embodiment, the support platform 415 is fixedly connected to the connecting plate 13, and the motor 416 connects the support platform 415 and the objective lens adapter 412. The motor 416 is used to drive the objective lens adapter 412 to move the objective lens 33 up and down along the optical axis. In this embodiment, the motor 416 can drive a load of approximately 625g to perform up and down adjustment with nanometer-level precision, thereby adjusting the distance between the object surface and the objective lens 33 by adjusting the position of the objective lens 33, so that the optimal / superior imaging state can be achieved throughout the entire detection process.

[0047] Please refer to the following: Figures 10-12 The beam splitter 36 also includes a fixed mounting surface 131 (see...). Figure 1The reference support 42 is located on the mounting surface 131. In this embodiment, the reference support 42 has a generally rectangular outer contour, including a bottom plate 421 and a top plate 422 that are parallel to each other, and side plates 423, 424, 425 and 426 that are sequentially connected between the bottom plate 421 and the top plate 422. Within the space enclosed by the bottom plate 421, the top plate 422, the side plates 423, 424, 425 and 426, a laser reference support 4271, a focusing reference support 4272 and a fluorescence reference surface 4273 are formed. The laser reference support 4271 and the focusing reference support 4272 are respectively connected to the mounting surface 131 (see...). Figure 1 The fluorescence reference surface 4273 is perpendicular to the mounting surface 131 and is at a 45° angle. The laser reference support 4271 is used to install and position (e.g., by dispensing) the first color separator 361, the focusing reference support 4272 is used to install and position (e.g., by dispensing) the second color separator 362, and the fluorescence reference surface 4273 is used to position the third color separator 363.

[0048] The base plate 421 has a circular objective lens opening 4211 for docking with the objective lens 33. The top plate 422 has a spaced-apart circular focusing opening 4221 and a strip-shaped mounting slot 4222. The focusing opening 4221 is used to dock with the autofocus assembly 35, and the mounting slot 4222 is used to mount the third dichroic filter 363. The side plate 423 has a circular, spaced-apart laser opening 4231 and a first fluorescence opening 4232. The laser opening 4231 is used to dock with the light source 31, and the first fluorescence opening 4232 is used to dock with the first fluorescence channel 301. The side plate 424 has a rectangular first mounting window 4241 and is provided with a first light-shielding and dustproof cover 4242 covering the first mounting window 4241. The first mounting window 4241 is reserved as a channel for mounting the second dichroic filter 362. The side plate 425 has a rectangular second mounting window 4251 and is provided with a second light-shielding and dustproof cover 4252 covering the second mounting window 4251. The second mounting window 4251 is reserved as a channel for installing the first color separator 361. The side plate 426 has a circular second fluorescence opening 4261. The second fluorescence opening 4261 is used to connect to the second fluorescence channel 302.

[0049] In this embodiment, a circular pin hole 4281 and a slotted pin hole 4282 are respectively provided on opposite sides of the bottom of the reference support 42. The circular pin hole 4281 is used for basic positioning of the reference support 42 in the entire system, and the slotted pin hole 4282 provides adjustment allowance for the pin, allowing for fine-tuning of the reference support 42 in the entire system and its installation direction. The two sides of the bottom of the reference support 42 with the circular pin hole 4281 and the slotted pin hole 4282 are also fixed to the mounting plate 13 by two screws 4291 and 4292 respectively. In this embodiment, the base plate 421 of the reference support 42 also has two finely machined stepped surfaces 4212 and 4213 for contacting and mounting with the reference mounting surface 132 at the corresponding position on the mounting plane 131.

[0050] In this embodiment, the entire dichroic filter (361, 362, 363), light source 31, objective lens 32, fluorescence channel (301), and autofocus assembly 35 are mounted and positioned using the reference support 42. This significantly simplifies the optical path frame structure, meets the system's beam splitting performance requirements, and shortens the optical path between the objective lens 32 and the fluorescent channel tube lens, reducing the design difficulty of the tube lens and enhancing the assurance of the system's imaging performance. Furthermore, by forming the laser reference support 4271 and the focusing reference support 4272, precise positioning of the first dichroic filter 361 and the second dichroic filter 362 can be achieved without the need for adjustment components for the first dichroic filter 361 and the second dichroic filter 362, making the entire reference support 42 and the entire system more compact and stable in structure.

[0051] Please refer to the following: Figure 12 and Figure 13 The optical path mounting and adjustment module 40 also includes a beam splitting adjustment component 43. The beam splitting adjustment component 43 is used to mount and fix the third color separator 363, and can realize fine-tuning of the angle of the third color separator 363 to ensure that the third color separator 363 is at 45° with the optical axis of the system.

[0052] In this embodiment, the beam splitting adjustment component 43 includes a fixing frame 431. The fixing frame 431 includes a frame structure 4311 and a connecting block 4312 that are fixedly connected. The frame structure 4311 forms a rectangular color separator mounting position 4313. The third color separator 363 can be fixed in the color separator mounting position 4313 by three-point or four-point glue injection, so as to avoid the stress of the glue causing the third color separator 363 to be squeezed and deformed, thus affecting the image quality. When the third color separator 363 is fixed in the color separator mounting position 4313, it is perpendicular to the connecting block 4312. The frame structure 4311 with the third color separator 363 fixed can be partially inserted into the reference support 42 through the mounting slot 4222, and the connecting block 4312 is located outside the reference support 42.

[0053] The spectroscopic adjustment assembly 43 also includes an adapter block 432 located on the side of the connecting block 4312 away from the reference support 42. The adapter block 432 is fixedly connected to the connecting block 4312 by four screws 4321, so that the connecting block 4312, located outside the reference support 42, is suspended relative to the top plate 422 of the reference support 42, that is, there is a gap between the connecting block 4312 and the reference support 42, and they are not in direct contact. The adapter block 432 is also fixedly connected to the top plate 422 of the reference support 42 by four screws 4322.

[0054] The spectral adjustment assembly 43 also includes an adjustment seat 433. The adjustment seat 433 is located on one side of the adapter block 432 and is arranged side by side with the adapter block 432. The adjustment seat 433 is fixedly connected to the top plate 422 of the reference support 42 by two screws 4331. A set screw hole 4332 is opened at each end of the adjustment seat 433. The two set screw holes 4332 penetrate the adjustment seat 433 in a direction perpendicular to the third color separator 363.

[0055] In this embodiment, a pin hole 4314 is provided on the connecting block 4312 as a rotation axis. When a set screw is inserted into the two set screw holes 4332, the two set screws can push the two ends of the side plate of the adapter block 432 respectively, thereby applying a pushing force to the corresponding point on the fixed frame 431, causing the third color separation piece 363 to axially deflect around the pin hole 4314. That is, by pushing the adapter block 432 with the set screws in the two set screw holes 4332, the angle between the third color separation piece 363 and the system optical axis can be adjusted. In this embodiment, it is necessary to adjust the third color separation piece 363 to form a 45° angle with the system optical axis. In addition, since the connecting block 4312 is suspended relative to the top plate 422 of the reference support 42 in this embodiment, the contact friction between the connecting block 4312 and the top plate 422 of the reference support 42 can be effectively avoided during the adjustment of the deflection of the third color separation piece 363, thus preventing the adjustment from being hindered. Furthermore, it also avoids affecting the surface shape of the third color separation 363 due to the deformation of the fixing frame 431 on which the third color separation 363 is installed.

[0056] Please refer to the following: Figure 14 and Figure 15The optical path installation and adjustment module 40 also includes a telescope adjustment assembly 44. The telescope adjustment assembly 44 includes a support and clamping mechanism 441 to ensure that the optical axis of the telescope 33 is aligned with the central optical axis of the entire system. Furthermore, the support and clamping mechanism 441 effectively ensures the reliability and stability of the telescope 33 installation, enhancing the overall system reliability. The base plate of the support and clamping mechanism 441 has a circular pin hole 442 and a slotted pin hole 443, which are spaced apart along the optical axis of the telescope 33, with the opening of the slotted pin hole 443 extending along the optical axis of the telescope 33. The circular pin hole 442 and the slotted pin hole 443 are used to precisely position the telescope within the entire optical path and in its installation direction; additionally, the slotted pin hole 443 provides adjustment margin along the optical axis of the telescope 33. The base plate of the supporting clamping mechanism 441 is also provided with two machined surfaces 444 for contacting and mounting with the reference mounting surface 132 at the corresponding position on the mounting plane 131. In this embodiment, the supporting clamping mechanism 441 is also fixed to the mounting plate 13 by four fastening screws 445.

[0057] Please refer to the following: Figures 16-18 The detection optical path module 30 also includes a camera adjustment assembly 37. A camera 34 is mounted and connected to the camera adjustment assembly 37, which is used to install, position, and adjust the position and angle of the camera 34. In this embodiment, the camera adjustment assembly 37 is used to achieve the mounting and fixing of the camera 34, as well as its translation and rotation in various directions. The camera adjustment assembly 37 includes a mounting base plate 371, a camera bracket 372, a camera fixing ring 373, a vertical adjustment member 374, a front-to-back adjustment member 375, and a rotation adjustment member 376. The mounting base plate 371 is fixedly connected to the mounting plate 13. The camera bracket 372 is fixedly connected to the surface of the mounting base plate 371 away from the mounting plate 13. The camera fixing ring 373 and the vertical adjustment member 374 are fixedly connected to the camera bracket 372. The camera fixing ring 373 is used to fix the camera 34. The vertical adjustment member 374 is used to adjust the vertical translation of the camera 34 in a direction perpendicular to the mounting plate 13. The front-to-back adjustment component 375 is connected to the mounting base plate 371 and is used to adjust the translation of the camera 34 in a direction perpendicular to the camera's photosensitive surface. The rotation adjustment component 376 is connected to the up-down adjustment component 374 and is used to adjust the rotation of the camera 34 around the optical axis.

[0058] In this embodiment, the mounting base plate 371 has a first surface 3711 and a second surface 3712 disposed opposite to each other. The first surface 3711 contacts the corresponding reference mounting surface 132 on the mounting plate 13 by forming two finely machined stepped surfaces, so that when the camera 34 is adjusted up and down, the camera 34 only moves in the Z direction, reducing the positional change in the XY direction. The mounting base plate 371 has four slots 3713 that penetrate the first surface 3711 and the second surface 3712, so as to fix the mounting base plate 371 to the mounting plate 13 by four screws 3714.

[0059] A set screw hole 3716 is respectively formed on the two opposite side plates 3715 of the mounting base plate 371. Each set screw hole 3716 extends in a direction parallel to the camera 34 and perpendicular to the optical axis of the telescope lens 33. A first guide groove 3717 is formed on the side of the mounting base plate 371 facing the camera 34. A front-to-back adjustment member 375 is disposed in the first guide groove 3717. The front-to-back adjustment member 375 includes a first fixing block 3751, two screws 3752 and a front-to-back adjustment screw 3753. The first fixing block 3751 is fixedly connected to the mounting plate 13 by the two screws 3752 on both sides (see Figure 1 The front-to-back adjusting screw 3753 is rotatably connected to the first fixed block 3751, with one end extending into the first guide groove 3717. Four slots 3713 on the mounting base plate 371 extend along the direction perpendicular to the camera's photosensitive surface to reserve adjustment space in that direction. By rotating the front-to-back adjusting screw 3753, one end of the screw located in the first guide groove 3717 can push or pull the mounting base plate 371, thereby adjusting the front-to-back translation of the mounting base plate 371 and causing the camera 34 to translate back and forth. In this way, the position of the camera 34 relative to the telescope lens 33 can be adjusted to be closer or farther, thus adjusting the photosensitive surface of the camera 34 to the back focal plane of the telescope lens 33.

[0060] The camera mount 372 includes an integrally formed base plate 3721 and a vertical plate 3722. The base plate 3721 has four spaced-apart slots 3723. The base plate 3721 is fixedly connected to a groove 3718 on the second surface 3712 via the four slots 3723 and four screws 3724. Each slot 3723 extends in a direction parallel to the camera's photosensitive surface. The vertical plate 3722 is connected to the side of the base plate 3721 away from the second surface 3712. The vertical plate 3722 has a circular mounting opening 3724 for mounting a camera mounting ring 373 and a camera 34. By inserting set screws into the two set screw holes 3716 of the mounting base plate 371, the base plate 3721 located in the groove 3718 can be pushed to move left and right (parallel to the photosensitive surface of the camera 34 and perpendicular to the optical axis of the tube lens 33), thereby causing the camera 34 to move left and right.

[0061] By using the above-mentioned mounting base 371 and camera bracket 372 structure, the left and right camera adjustment seat in the prior art is eliminated, saving parts costs and reducing the complexity of the entire adjustment structure.

[0062] The up-and-down adjustment component 374 includes an adjustment plate 3741, a second fixing block 3742, two screws 3743, an up-and-down adjustment screw 3744, and four screws 3737. The adjustment plate 3741 is a rectangular thin plate structure with a slot 3746 at each of its four corners. Each slot 3746 extends in the Z direction to allow for adjustment space in the Z direction. The adjustment plate 3741 is fixedly connected to the surface of the upright plate 3722 facing the camera 34 by the four screws 3737 and the four slots 3746. A second guide groove 3747 is formed on the top of the adjustment plate 3741. The two screws 3743 fix the second fixing block 3742 to the top of the upright plate 3722. The up-and-down adjustment screw 3744 is rotatably connected to the second fixing block 3742, with one end extending into the second guide groove 3747. By rotating the up-down adjusting screw 3744, one end of the up-down adjusting screw 3744 located in the second guide groove 3747 can push or pull the adjusting plate 3741, thereby adjusting the up-down translation of the adjusting plate 3741 and driving the camera 34 to translate up-down.

[0063] The camera retaining ring 373 is located within the rotating adjustment member 376, and the camera is clamped between the camera retaining ring 373 and the rotating adjustment member 376. The rotating adjustment member 376 has a clamping structure. The rotating adjustment member 376 has four fixing holes 3761, which are arranged at intervals in the circumferential direction. It can be fixedly connected to the surface of the adjustment plate 3741 facing the camera 34 by four screws 3762 and the four fixing holes 3761. The rotating adjustment member 376 also has three pin holes 3763 for basic positioning of the camera 23, which are arranged at equal intervals in the circumferential direction. The rotating adjustment member 376 also has a flange 3764 extending towards the camera 34. A locking hole 3765 is provided at the end of the flange 3764 away from the mounting base plate 371. By tightening the screws 3766, the rotating adjustment member 376 can clamp the camera 34; when the screws 3766 are loosened, the camera 34 can be rotated and adjusted. In this embodiment, a pin hole 3767 is provided at the end of the flange 3764 away from the mounting base plate 371. When the screw 3766 is loosened, the camera 34 can be adjusted by rotating around the pin hole 3767.

[0064] The camera adjustment component 37 described in this application can realize the forward and backward, left and right, up and down translation and rotation of the camera 34.

[0065] Please refer to this again. Figure 3 and Figure 4The optical inspection device 100 in this embodiment also includes a vibration damping module 50. The vibration damping module 50 is located on the side of the frame 12 away from the mounting plate 13, and is used to support the main support assembly 10, the stage module 20, and the detection optical path module 30. The vibration damping module 50 includes a base plate 51 and four support columns 52 fixedly connected to the same surface of the base plate 51. The four support columns 52 are respectively supported and connected to the frame 12, so that the main support assembly 10 is suspended relative to the base plate 51. Each support column 52 includes a vibration damping component 521 that directly contacts the frame 12. The overall design of the vibration damping module 50 can support a weight of over 67 kg, and can isolate vibration frequencies above 8 Hz, 15 Hz, 20 Hz, or 30 Hz depending on different vibration damping requirements.

[0066] The optical inspection device described in this application includes a stage module, which comprises a moving platform, a reagent kit, and a slide processing device. Specifically, the stage module integrates the reagent kit and slide processing device, which can move synchronously with the moving platform to meet optical inspection requirements. Therefore, the optical inspection device of this application has a high degree of integration, which helps reduce the overall size of the device and facilitates transportation, installation, and optical inspection operations. Furthermore, the integration of the reagent kit and slide processing device into the stage module eliminates the need for additional installation space, thus improving space utilization.

[0067] Those skilled in the art should recognize that the above embodiments are only used to illustrate this application and are not intended to limit this application. Any appropriate changes and variations made to the above embodiments within the essential spirit and scope of this application fall within the scope of protection claimed in this application.

Claims

1. An optical inspection device, characterized in that, include: The main support module includes a fixedly connected frame and a mounting plate, which together form a detection space; A stage module, located within the detection space, includes a mobile platform and a slide processing device integrated within the mobile platform. The mobile platform has a bearing surface facing the mounting plate for bearing biological slides. The mobile platform also has mounting positions for accommodating reagent kits, which hold reaction reagents. The slide processing device is used to load the reaction reagents onto the biological slides. The detection optical path module, mounted on the mounting plate, is used to emit excitation light to irradiate the biological sample on the biological slide and to collect the light signal generated by the biological sample under the irradiation of the excitation light. The mobile platform is movable within the mobile space along the optical axis perpendicular to the detection optical path module to synchronously drive the slide processing device and the reagent kit to translate; the detection optical path module includes an objective lens movable along the optical axis, and the light signal is collected by the objective lens; the excitation light is projected onto different positions on the biological slide as the mobile platform translates to scan the biological sample.

2. The optical inspection device as described in claim 1, characterized in that, It also includes an optical path mounting and adjustment module connected to the mounting plate, used to position the detection optical path module and adjust the position and angle of the detection optical path module.

3. The optical inspection device as described in claim 2, characterized in that, The optical path mounting adjustment module includes an objective lens adjustment assembly; The objective lens adjustment assembly includes an objective lens holder, an objective lens adapter, and a wedge-shaped adjustment ring that are fixedly connected. The objective lens is engaged in the objective lens holder, which is located within a mounting opening on the objective lens adapter. The wedge-shaped adjustment ring extends and is sandwiched between the objective lens holder and the objective lens adapter, and the top surface of the wedge-shaped adjustment ring facing the objective lens adapter is a wedge-shaped surface.

4. The optical inspection device as described in claim 3, characterized in that, The objective lens retainer includes an engaging portion and a side edge that are connected to each other. The engaging portion is a cylindrical structure for fixing the objective lens, and the side edge is formed by extending from one end of the engaging portion away from the objective lens adapter in a direction away from the objective lens. The wedge-shaped adjustment ring includes an interconnected ring portion and a wedge-shaped portion. The ring portion surrounds the periphery of the side edge, and the wedge-shaped portion extends from one end of the ring portion near the objective lens adapter toward the objective lens. The wedge-shaped portion surrounds the periphery of the engaging portion and extends between the side edge and the objective lens adapter. The side edge, the wedge-shaped portion, and the objective lens adapter are fixedly connected. The top surface of the wedge-shaped portion facing the objective lens adapter is a wedge-shaped surface.

5. The optical inspection device as described in claim 2, characterized in that, The optical signal includes a fluorescence signal in response to the excitation light and a focusing light signal; the detection optical path module includes a fluorescence detection module and a focusing module optically connected to the objective lens, and a beam splitter assembly disposed between the objective lens and the fluorescence detection module and the focusing module. The beam splitter assembly includes at least a reference support and a first dichroic plate and a second dichroic plate installed within the reference support. The first dichroic plate is used to reflect the excitation light to the objective lens and transmit the optical signal, and the second dichroic plate is used to reflect the fluorescence signal to the fluorescence detection module and transmit the focusing light signal to the focusing module. The reference support is installed on the mounting plate and includes a space formed by a top plate, a bottom plate, and multiple side plates. The reference support also forms a laser reference support and a focusing reference support located in the space. The laser reference support is used to position the first color separation piece, and the focusing reference support is used to position the second color separation piece, so that the first color separation piece and the second color separation piece are respectively at a 45° angle to the surface of the mounting plate.

6. The optical inspection device as described in claim 5, characterized in that, The fluorescence detection module includes a first fluorescence acquisition channel and a second fluorescence acquisition channel, which respectively acquire fluorescence signals of different wavelengths; the spectrophotometer also includes a third color separator. The base plate is located between the mounting plate and the top plate. The top plate has a mounting slot. The third color separator is inserted into the space formed by the reference support through the mounting slot. The third color separator is perpendicular to the mounting plate and is used to selectively reflect the fluorescence signal to the first fluorescence acquisition channel or transmit it to the second fluorescence acquisition channel according to the wavelength.

7. The optical inspection device as described in claim 6, characterized in that, The beam-splitting component also includes a beam-splitting adjustment component; The spectral adjustment component is connected to the mounting slot on the top plate, used to fix the third color separator, and to adjust the rotation of the third color separator to adjust the angle between the third color separator and the fluorescence signal.

8. The optical inspection device as described in claim 7, characterized in that, The spectral modulation component includes: A fixed frame includes a frame structure and a connecting block. The frame structure is located within the space formed by the reference support and is used to fix the third color separation piece. The connecting block is located outside the reference support. An adapter block, located on the side of the connecting block away from the top plate, is fixedly connected to both the bottom plate and the connecting block. The adapter block has pin holes. An adjusting seat is arranged side by side with the adapter block and fixedly connected to the top plate. Each end of the adjusting seat has a set screw hole. By inserting a set screw into the set screw hole, the two ends of the adapter block can be pushed, so that the adapter block drives the fixed frame and the third color separation piece to sway axially around the pin hole.

9. The optical inspection device as described in claim 5, characterized in that, The fluorescence detection module includes a telescope, and the optical path mounting and adjustment module includes a telescope adjustment component. The telescope adjustment assembly is fixedly connected to the mounting plate and includes a support and clamping mechanism for supporting and fixing the telescope. The support and clamping mechanism has a circular pin hole and a slotted pin hole on the surface facing the mounting plate. The circular pin hole is used to position the telescope, and the slotted pin hole is used to reserve adjustment margin for the telescope along the optical axis of the fluorescence signal.

10. The optical inspection device as described in claim 6, characterized in that, Both the first fluorescence acquisition channel and the second fluorescence acquisition channel include a camera and a camera adjustment assembly. The camera adjustment assembly is used to position the camera and to adjust the translation and rotation of the camera. The camera adjustment assembly includes a mounting base and front and rear adjustment components. The mounting base plate is connected to the mounting plate, and the front and rear adjustment components are used to push or pull the mounting base plate to move back and forth in a direction perpendicular to the photosensitive surface of the camera, thereby driving the camera to move back and forth synchronously.

11. The optical inspection device as described in claim 10, characterized in that, The mounting base plate has a groove formed on its surface away from the mounting plate, and the camera adjustment assembly also includes a camera bracket connected in the groove, with the camera mounted on the camera bracket; A set screw hole is provided on each of the two opposite side plates of the mounting base. By inserting a set screw into the set screw hole, the camera bracket can be pushed to move left and right in a direction parallel to the photosensitive surface of the camera, thereby driving the camera to move left and right synchronously.

12. The optical inspection device as described in claim 11, characterized in that, The camera adjustment assembly further includes a camera fixing ring and a rotation adjustment component. The camera is clamped between the camera fixing ring and the rotation adjustment component, and the rotation adjustment component is used to control the camera to rotate around an axis.

13. The optical inspection device as described in claim 12, characterized in that, The rotary adjustment component has a flange extending toward the camera. The end of the flange away from the mounting base plate has a locking hole and a pin hole. When the screw in the locking hole is tightened, the rotary adjustment component can hold the camera. When the screw is loosened, the camera can be adjusted to rotate axially around the pin hole.

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