A bactericidal composition and its use

Abstract

This invention relates to a fungicide composition comprising active ingredient A and active ingredient B. Active ingredient A is a compound of formula (I), and active ingredient B is any one of boscalid, isothiazamide, bifenazate, and azoxystrobin. The mass ratio of active ingredient A to active ingredient B is 1:25 to 32:1. The fungicide composition of this invention exhibits excellent control effects against various plant pathogens. Under certain mass ratios, its control effect is synergistic, effectively reducing pesticide dosage, lowering agricultural input costs, and delaying the development of drug resistance in plant pathogens.

Description

Technical Field

[0001] This invention belongs to the field of pesticide compound technology, specifically relating to a bactericidal composition and its application. Background Technology

[0002] Compound (I) (Carmeconazole) is a triazole fungicide developed by Kureha Chemical Co., Ltd. of Japan. CAS Registry Number: 2323565-15-5. It exhibits high fungicidal activity against a variety of important pathogens, such as wheat scab and rice blast.

[0003] Boscalid is a mitochondrial respiration inhibitor that controls fungal hyphal growth by inhibiting the activity of succinate-coenzyme Q reductase in the mitochondrial electron transport chain. Its mechanism of action is similar to that of other amide and benzamide fungicides.

[0004] Isofetamid is a novel fungicide developed by Ishihara Sangyo Co., Ltd. of Japan. Due to its chemical structure being similar to existing succinate dehydrogenase inhibitors (SDHIs), this product is classified as SDHI under FRAC code 7.

[0005] Pyraziflumid is a novel fungicide developed by Nippon Nippon Kogyo Co., Ltd. It works by inhibiting the activity of succinate dehydrogenase (SDH) in mitochondrial complex II, thereby blocking the energy metabolism of pathogens, inhibiting their growth, and ultimately causing their death, thus achieving the goal of controlling plant diseases.

[0006] Aminopyrifen is a pyridine fungicide developed by Sumitomo Chemical Co., Ltd. of Japan. This compound has a diphenyl ether structure and its fungicidal mechanism is novel. It inhibits the activity of acyltransferase (GWT-1), thereby affecting the biosynthesis of glycosylphosphatidylinositol (GPI) on the endoplasmic reticulum membrane and terminating fungal reproduction.

[0007] This invention combines the compound of formula (I) with boscalid, isothiazinam, bifenazate, and azoxystrobin, and surprisingly found that, under certain mass ratios, it has a synergistic effect on strawberry gray mold and other diseases, which can significantly reduce the amount of chemical pesticides used and reduce the risk of pathogens developing resistance to single agents. Summary of the Invention

[0008] Based on the above, the purpose of this invention is to provide a bactericidal composition and its application. This bactericidal composition, through the compounding of different active ingredients, exhibits excellent control effects against a variety of plant pathogens. Under certain mass ratios, the control effect shows a synergistic effect, which can effectively reduce the amount of pesticides used, lower agricultural input costs, and delay the development of drug resistance in plant pathogens.

[0009] To achieve the above objectives, the present invention adopts the following technical solution: a bactericidal composition, wherein the bactericidal composition comprises active ingredient A and active ingredient B, wherein active ingredient A is a compound of formula (I), with the following chemical structural formula:

[0010] (I) The active ingredient B is any one of cyprodinil, isothiazinamide, bifenpyrazinamide, and azoxystrobin.

[0011] Furthermore, the mass ratio of active ingredient A to active ingredient B is 1:25 to 32:1;

[0012] Furthermore, the mass ratio of the compound of formula (I) to cyprodinil is 1:18 to 18:1;

[0013] Furthermore, the mass ratio of the compound of formula (I) to cyazofamid is 1:18, 1:10, 2:11, 3:8, 2:1, 8:3, 11:2, 10:1, or 18:1;

[0014] Furthermore, the mass ratio of the compound of formula (I) to isothiazine is 1:18 to 25:1;

[0015] Furthermore, the mass ratio of the compound of formula (I) to isothiazamide is 1:18, 1:12, 1:8, 1:5, 5:4, 5:1, 10:1, 15:1, or 25:1;

[0016] Furthermore, the mass ratio of the compound of formula (I) to bifenpyrazinamide is 1:18 to 32:1;

[0017] Furthermore, the mass ratio of the compound of formula (I) to bifenpyrazinamide is 1:18, 1:10, 1:6, 1:3, 1:1, 3:1, 6:1, 10:1, 18:1, or 32:1;

[0018] Furthermore, the mass ratio of the compound of formula (I) to azoxystrobin is 1:25 to 24:1;

[0019] Furthermore, the mass ratio of the compound of formula (I) to azoxystrobin is 1:25, 1:18, 1:10, 2:11, 2:7, 5:3, 3:1, 8:1, 15:1, or 24:1;

[0020] Furthermore, the total weight of the bactericidal composition is 100 wt%, and the sum of the contents of active ingredient A and active ingredient B in the bactericidal composition is any value between 1 and 90 wt% or more.

[0021] Furthermore, based on a total weight of 100 wt% for the bactericidal composition, the sum of the contents of active ingredient A and active ingredient B in the bactericidal composition is 2-50 wt%.

[0022] Furthermore, the bactericidal composition includes agriculturally acceptable auxiliary ingredients in addition to the active ingredients;

[0023] Furthermore, the auxiliary components include one or more of the following: wetting agents, dispersants, emulsifiers, thickeners, disintegrants, antifreeze agents, defoamers, solvents, stabilizers, film-forming agents, warning colors, penetrants, and carriers;

[0024] Further, the wetting agent is selected from one or more of the following: sodium dodecylbenzene sulfate, sodium dodecylbenzene sulfonate, soapberry powder, alkyl sulfate, pull-apart powder BX, silkworm excrement, alkylphenol polyoxyethylene ether, fatty alcohol polyoxyethylene ether, alkanolamide polyoxyethylene ether and its phosphate or sulfate salts, alkyl polyoxyethylene ether succinate sulfonate.

[0025] Further, the dispersant is selected from one or more of the following: polycarboxylate, lignin sulfonate, naphthalene or alkylnaphthalene formaldehyde condensate sulfonate, calcium alkylbenzene sulfonate, alkylphenol polyoxyethylene phosphate, fatty alcohol polyoxyethylene polyoxypropylene ether, fatty alcohol polyoxypropylene polyoxypropylene ether, and polymeric alkyl aryl sulfonate.

[0026] Further, the emulsifier is selected from one or more of the following: fatty alcohol polyoxyethylene ether, fatty alcohol ethylene oxide-propylene oxide copolymer, phenethylphenol polyoxyethylene polyoxypropylene ether, alkylphenol polyoxyethylene ether, fatty amine polyoxyethylene ether, alkylbenzene sulfonate, styrene-phenol polyoxyethylene ether, and fatty acid polyoxyethylene ester.

[0027] Furthermore, the thickener is selected from one or more of xanthan gum, polyvinyl alcohol, organobentonite, magnesium aluminum silicate, and carboxymethyl cellulose;

[0028] Furthermore, the disintegrant is selected from one or more of the following: aluminum chloride, bentonite, sucrose, modified starch, cellulose, urea, sodium carbonate, sodium bicarbonate, sodium chloride, sodium sulfate, citric acid, and tartaric acid.

[0029] Furthermore, the antifreeze is selected from one or more of alcohols, alcohol ethers, and inorganic salts, and is a mixture thereof;

[0030] Furthermore, the defoamer is selected from silicone oil, C 10 ~C 20 Saturated fatty acid compounds, C8-C 10 A mixture of one or more of fatty alcohols or silicone compounds;

[0031] Further, the solvent is selected from one or more of the following: benzene, toluene, xylene, methanol, ethanol, isopropanol, n-butanol, diesel oil, N,N-dimethylformamide, cyclohexanone, ethyl acetate, N-methylpyrrolidone, propanol, butanol, ethylene glycol, diethylene glycol, ethylene glycol methyl ether, butyl ether, solvent oil, vegetable oil, vegetable oil derivatives, and deionized water;

[0032] Furthermore, the preservative is selected from one or more of the following: sorbic acid, sodium sorbate, potassium sorbate, benzoic acid, sodium benzoate, sodium p-hydroxybenzoate, and methyl p-hydroxybenzoate;

[0033] Furthermore, the stabilizer is selected from one or more of oxalic acid, succinic acid, adipic acid, borax, and epoxidized vegetable oil;

[0034] Furthermore, the warning color is selected from any one or more of the following adjustment colors: blue, green, red, purple, and yellow;

[0035] Furthermore, the film-forming agent is selected from one or more of sodium carboxymethyl starch, sodium carboxymethyl cellulose, sodium alginate, polyvinyl alcohol, and polyacrylic acid;

[0036] Furthermore, the carrier is selected from one or more of the following: kaolin, bentonite, attapulgite, light calcium carbonate, diatomaceous earth, and precipitated silica.

[0037] Furthermore, the bactericidal composition can be prepared into any formulation permitted in agriculture, wherein the formulation is a solid formulation, a liquid formulation, or a seed treatment formulation;

[0038] Furthermore, the solid dosage form is a wettable powder or a water-dispersible granule;

[0039] Furthermore, the liquid formulation is a suspension, emulsifiable concentrate, water emulsion, or microemulsion, and the seed treatment formulation is a seed treatment suspension.

[0040] A method for preventing or controlling plant pathogens from infecting plants, comprising using a bactericidal composition containing a melanin biosynthesis inhibitor to control pathogens in agricultural, forestry or horticultural plants.

[0041] Furthermore, the plant pathogen mentioned is strawberry gray mold.

[0042] Furthermore, the bactericidal composition is applied in an effective amount to plant pathogens and / or their environment, or to plants, plant propagation material and subsequently grown plant organs, soil or cultivation medium, material or space.

[0043] Beneficial effects:

[0044] Compared with the prior art, the present invention has the following advantages: the bactericidal composition of the present invention not only expands the spectrum of prevention and control, but also extends the service life of the agent and broadens the appropriate period for prevention and control; it can delay the development of resistance in pathogens; and it has excellent prevention and control effects under certain mass ratios, with obvious synergistic effects. Detailed Implementation

[0045] To better illustrate the effective prevention and control effects of the present invention, the agents described in the above embodiments are used. To make the technical solution, purpose, and advantages of the present invention clearer, the present invention is illustrated with the following specific embodiments, but the present invention is not limited to these examples.

[0046] Formulation preparation examples:

[0047] Preparation Example 1: 33% of formula (I) compound · cymoxanil water-dispersible granules (24:9)

[0048] Formula composition: 24% compound of formula (I), 9% cymoxanil, 3.5% sodium dodecyl sulfate, 4% polynaphthalene sulfonate, 1% sodium fatty alcohol polyoxyethylene ether sulfate, 6% sodium polycarboxylate, 4.5% sodium lignosulfonate, 5% attapulgite, and kaolin to make up the balance;

[0049] Preparation method: According to the formula ratio, the active ingredients are added to the carrier, and surfactants and other functional additives are added to it. After mixing, the mixture is pulverized by air jet and 10-25% water is added. Then, the mixture is kneaded, granulated, dried and sieved to obtain the water-dispersible granule product.

[0050] Preparation Example 2: 28% Compound (I)·Benomylamide Suspension (20:8)

[0051] Formula composition: 20% of compound (I), 8% cyazofamid, 2.5% phenethylphenol polyoxyethylene polyoxypropylene ether, 2% Gelbert alcohol polyoxyethylene ether, 3% styrene phenol polyoxyethylene ether phosphate, 0.25% xanthan gum, 0.8% magnesium aluminum silicate, 5% ethylene glycol, 0.1% potassium benzoate, 0.5% organosilicon defoamer, deionized water to make up the balance;

[0052] Preparation method: According to the formula ratio, the active ingredients, surfactants and other functional additives are placed in the reaction vessel in sequence, water is added and mixed evenly, and then subjected to high-speed shearing, wet sand milling and finally homogenization filtration to obtain the suspension.

[0053] Preparation Example 3: 50% of formula (I) compound·pyridaben wettable powder (40:10)

[0054] Formula composition: 40% of compound (I), 10% cymoxanil, 5% sodium lignosulfonate, 4% naphthalene sulfonate formaldehyde condensate, 4% alkyl naphthalene sulfonate, 2.5% sodium dodecyl sulfate, 5% kaolin, 10% starch, and bentonite to make up the balance;

[0055] Preparation method: Mix the active ingredients, wetting agent, dispersant and filler according to the formula ratio, mechanically pulverize and then air-jet pulverize until uniformly mixed to obtain a wettable powder product.

[0056] Preparation Example 4: 30% of Formula (I) compound · isopyram water-dispersible granules (25:5)

[0057] Formula composition: 25% compound of formula (I), 5% isopropylthiocarbamate, 6.5% polynaphthalene sulfonate, 3.5% sodium dodecyl sulfate, 1% sodium fatty alcohol polyoxyethylene ether sulfate, 2% sodium polycarboxylate, 3% sodium lignosulfonate, 5% starch, and attapulgite to make up the balance.

[0058] Preparation method: Same as in preparation example 1.

[0059] Preparation Example 5: 20% Formula (I) compound·isoprothiazide suspension (15:5)

[0060] Formula composition: 15% compound of formula (I), 5% isopropylthiolam, 5% styrene-phenol polyoxyethylene ether phosphate, 2.5% phenethylphenol polyoxyethylene polyoxypropylene ether, 0.25% xanthan gum, 0.8% magnesium aluminum silicate, 5% ethylene glycol, 0.1% potassium benzoate, 0.5% silicone oil, deionized water to make up the balance;

[0061] Preparation method: Same as in preparation example 2.

[0062] Preparation Example 6: 46% of Formula (I) compound · isopyram wettable powder (38:8)

[0063] Formula composition: 38% compound of formula (I), 8% isopyram, 5% sodium lignosulfonate, 4% naphthalene sulfonate formaldehyde condensate, 4% alkyl naphthalene sulfonate, 1.2% bleaching powder BX, 2.5% sodium dodecyl sulfate, 5% sucrose, 3% starch, kaolin to make up the balance;

[0064] Preparation method: Same as in preparation example 3.

[0065] Preparation Example 7: 30% of Formula (I) compound·bifenpyrazinamide suspension (24:6)

[0066] Formula composition: 24% compound of formula (I), 6% bifenthion pyrazinamide, 4.5% phenylethyl phenol polyoxyethylene polyoxypropylene ether, 2% Gelbert alcohol polyoxyethylene ether, 0.25% xanthan gum, 0.5% magnesium aluminum silicate, 5% ethylene glycol, 0.1% potassium benzoate, 0.5% organosilicon defoamer, deionized water to make up the balance;

[0067] Preparation method: Same as in preparation example 2.

[0068] Preparation Example 8: 47% of Formula (I) compound·bifenpyrazin wettable powder (35:12)

[0069] Formula composition: 35% compound of formula (I), 12% bifenthrin pyrazinamide, 5% sodium lignosulfonate, 4% naphthalene sulfonate formaldehyde condensate, 4% alkyl naphthalene sulfonate, 2.5% sodium dodecyl sulfate, 1.5% splitting powder BX, 5% kaolin, 15% starch, and bentonite to make up the balance;

[0070] Preparation method: Same as in preparation example 3.

[0071] Preparation Example 9: 32% Compound (I)·Ampicillin Suspension (20:12)

[0072] Formula composition: 20% compound of formula (I), 12% azoxystrobin, 2.5% phenethylphenol polyoxyethylene polyoxypropylene ether, 2% gerbert alcohol polyoxyethylene ether, 3% styrene phenol polyoxyethylene ether phosphate, 0.25% xanthan gum, 0.8% magnesium aluminum silicate, 5% ethylene glycol, 0.1% potassium benzoate, 0.5% silicone oil, deionized water to make up the balance;

[0073] Preparation method: Same as in preparation example 2.

[0074] Indoor activity test

[0075] Example 1:

[0076] Indoor combined treatment experiment for strawberry gray mold

[0077] Experimental subject: Strawberry gray mold (Botrytis cinerea Pers);

[0078] Experimental crop: Strawberry (Sweet Treasure);

[0079] Major instruments and equipment: 0.001 g electronic balance, pipettes, graduated cylinders, petri dishes, filter paper, spray equipment, climate incubator, etc.

[0080] Preparation of spore suspension: Fresh spores on PDA medium covered with gray mold were washed with a mixture of 0.05% Tween-80, 1% yeast extract, 2.5% glucose and 10% carrot juice. The spores were filtered through double gauze to prepare a spore suspension (about 1000 spores per 10x microscope field).

[0081] Reagent preparation: If the test reagent is water-soluble or processed into a formulation, it can be directly diluted with water. The original drug needs to be prepared into a 2.5% formulation for later use. Insoluble reagents need to be dissolved by ultrasonic vibration, and then an appropriate amount of 0.5% Tween-80 water is added according to the test concentration.

[0082] Chemical treatment: Select potted strawberry seedlings with uniform growth, cut leaves from the same position, and thoroughly soak the leaves in the pre-prepared chemical solution for 30 seconds. Drain off excess solution, allow to air dry naturally, and then incubate under moist conditions according to the treatment labels. A blank control was set up, containing only the solvent and no active ingredient.

[0083] Inoculation and cultivation: Spray the spore suspension evenly onto the leaf surface. For protective tests, inoculation is generally performed approximately 24 hours after treatment; for therapeutic tests, inoculation is generally performed 24 hours before treatment. Transfer to a climate incubator and incubate at 23°C for 2–3 days.

[0084] Grading Standards

[0085] Level 0: No symptoms;

[0086] Level 1: Less than 10%;

[0087] Level 3: 11%~25%;

[0088] Level 5: 26%~50%;

[0089] Level 7: 51%~75%;

[0090] Level 9: 76% or higher.

[0091] Calculation of drug efficacy:

[0092] Disease index = [∑(number of diseased leaves at each level × relative level value) / (total number of leaves surveyed × highest level)] × 100;

[0093] Prevention and control effect (%) = [(CK-PT) / CK] × 100;

[0094] In the formula, CK is the disease index of the blank control area; PT is the disease index of the drug-treated area.

[0095] Statistical analysis: Based on the survey results, regression analysis was performed on the data using a data processing system to calculate the EC50 of each drug. 50 The 95% confidence limit was set, and a significance analysis of the differences between the various drug treatments was performed.

[0096] The co-toxicity coefficient (CTC) of the mixture was calculated using Sun Yunpei's method to evaluate the type of combined effects.

[0097] A CTC ≥ 120 indicates a synergistic effect, a CTC ≤ 80 indicates an antagonistic effect, and a CTC between 80 and 120 indicates an additive effect.

[0098] The co-toxicity coefficient (CTC value) of the mixture is calculated using the following formula:

[0099] ATI = (S / M) * 100

[0100] In the formula:

[0101] ATI – Actual Measured Toxicity Index of Mixtures;

[0102] S – EC of standard bactericides 50 The unit is milligrams per liter (mg / L);

[0103] M – EC of the mixture 50 The unit is milligrams per liter (mg / L).

[0104] TTI=TI A *P A +TI B *P B

[0105] In the formula:

[0106] TTI – Theoretical Toxicity Index of Mixtures;

[0107] TI A —A. Toxicity index of drug A;

[0108] P A —The percentage content of drug A in the mixture, expressed as a percentage (%).

[0109] TI B —Toxicity index of drug B;

[0110] P B —Percentage content of agent B in the mixture, expressed as percentage (%).

[0111] CTC = (ATI / TTI) * 100

[0112] In the formula:

[0113] CTC – Cotoxicity Coefficient;

[0114] ATI – Actual Measured Toxicity Index of Mixtures;

[0115] TTI – Theoretical Toxicity Index of Mixtures.

[0116] Indoor test results:

[0117] Table 1 shows that each tested agent had varying degrees of control effect against strawberry gray mold. Among them, compound (I) showed the EC50 control effect against strawberry gray mold. 50 The EC50 concentration of boscalid against strawberry gray mold was 5.4668 mg / L. 50 The concentration was 1.8796 mg / L; when the mass ratio of compound (I) to cyprodinil was 1:18 to 18:1, it had a synergistic effect on gray mold in strawberries.

[0118] Table 1. Results of indoor combined effects of compound (I) and cyprodinil on strawberry gray mold.

[0119] Mass ratio Regression equation (y=) R <![CDATA[EC 50 (mg / L)]]> ATI TTI CTC interaction Compound A of formula (I) y = 3.9531 + 1.4191x 0.9987 5.4668 100 - - - Boscalid B y = 4.6065 + 1.4357x 0.9986 1.8796 290.85 - - - 1:25 y = 4.6720 + 1.4091x 0.9944 1.709 319.88 283.51 112.83 Additive effect 1:18 y = 4.7382 + 1.4065x 0.9981 1.5352 356.10 280.80 126.81 Enhancement effect 1:10 y = 4.7783 + 1.4346x 0.9987 1.4275 382.96 273.50 140.02 Enhancement effect 2:11 y = 4.8058 + 1.3731x 0.9976 1.3848 394.77 261.49 150.97 Enhancement effect 3:8 y = 4.8120 + 1.5629x 0.9995 1.3190 414.47 238.80 173.56 Enhancement effect 2:1 y = 4.8273 + 1.5427x 0.9993 1.2941 422.44 227.23 185.90 Enhancement effect 8:3 y = 4.6560 + 1.3518x 0.9986 1.7968 304.25 152.05 200.10 Enhancement effect 11:2 y = 4.4401 + 1.4490x 0.9953 2.4344 224.56 129.36 173.59 Enhancement effect 10:1 y = 4.3077 + 1.4656x 0.9958 2.9673 184.23 117.35 156.99 Enhancement effect 18:1 y = 4.1290 + 1.5641x 0.9916 3.6048 151.65 110.04 137.81 Enhancement effect 30:1 y = 3.9580 + 1.6049x 0.9933 4.4593 122.59 106.16 115.48 Additive effect

[0120] Table 2 shows the results of the EC50 control of isothiazine against strawberry gray mold. 50 The concentration was 0.9290 mg / L; when the mass ratio of compound (I) to isothiazine was 1:18 to 25:1, it had a synergistic effect on gray mold in strawberries.

[0121] Table 2. Results of indoor combined effects of compound (I) and isothiazamide on gray mold in strawberries.

[0122] Mass ratio Regression equation (y=) R <![CDATA[EC 50 (mg / L)]]> ATI TTI CTC interaction Compound A of formula (I) y = 3.9531 + 1.4191x 0.9987 5.4668 100 - - - Isoprophiazone B y = 5.0441 + 1.3793x 0.9981 0.9290 588.46 - - - 1:30 y = 5.0309 + 1.4765x 0.9975 0.9529 573.70 611.41 93.83 Additive effect 1:25 y = 5.1126 + 1.4793x 0.9982 0.8392 651.43 569.67 114.35 Additive effect 1:18 y = 5.1273 + 1.3435x 0.9953 0.8039 680.03 562.75 120.84 Enhancement effect 1:12 y = 5.2071 + 1.4236x 0.9981 0.7154 764.16 550.89 138.71 Enhancement effect 1:8 y = 5.2821 + 1.5295x 0.9961 0.6540 835.90 534.19 156.48 Enhancement effect 1:5 y = 5.3188 + 1.4576x 0.9953 0.6044 904.50 507.05 178.38 Enhancement effect 5:4 y = 5.0112 + 1.3884x 0.9984 0.9815 556.98 317.09 175.65 Enhancement effect 5:1 y = 4.7192 + 1.4680x 0.9936 1.5533 351.95 181.41 194.01 Enhancement effect 10:1 y = 4.4486 + 1.5829x 0.9961 2.2302 245.13 144.41 169.75 Enhancement effect 15:1 y = 4.4191 + 1.3340x 0.9982 2.7258 200.56 130.53 153.65 Enhancement effect 25:1 y = 4.2724 + 1.3657x 0.9978 3.4101 160.31 118.79 134.96 Enhancement effect

[0123] Table 3 shows the results of the EC50 control of bifenazate against strawberry gray mold. 50 The concentration was 2.0141 mg / L; when the mass ratio of compound (I) to bifenpyrazinamide was 1:18 to 32:1, it had a synergistic effect on gray mold in strawberries.

[0124] Table 3. Results of indoor combined effects of compound (I) and bifenazate on strawberry gray mold.

[0125] Mass ratio Regression equation (y=) R <![CDATA[EC 50 (mg / L)]]> ATI TTI CTC interaction Compound A of formula (I) y = 3.9531 + 1.4191x 0.9987 5.4668 100 - - - Bifenazate B y = 4.5502 + 1.4793x 0.9982 2.0141 271.43 - - - 1:32 y = 4.5641 + 1.4833x 0.9980 1.9674 277.87 266.23 104.37 Additive effect 1:18 y = 4.6344 + 1.5650x 0.9991 1.7125 319.23 262.40 121.66 Enhancement effect 1:10 y = 4.6730 + 1.5732x 0.9993 1.6138 338.75 255.84 132.41 Enhancement effect 1:6 y = 4.7145 + 1.5495x 0.9989 1.5285 357.66 246.94 144.84 Enhancement effect 1:3 y = 4.7584 + 1.5942x 0.9986 1.4176 385.64 228.57 168.72 Enhancement effect 1:1 y = 4.6792 + 1.5272x 0.9995 1.6221 337.02 185.71 181.47 Enhancement effect 3:1 y = 4.5607 + 1.4587x 0.9995 2.0006 273.26 142.86 191.28 Enhancement effect 6:1 y = 4.4071 + 1.4804x 0.9995 2.5148 217.39 124.49 174.62 Enhancement effect 10:1 y = 4.3606 + 1.3310x 0.9983 3.0228 180.85 115.58 156.47 Enhancement effect 18:1 y = 4.2192 + 1.3118x 0.9979 3.9371 138.85 109.02 127.36 Enhancement effect 32:1 y = 4.1831 + 1.3047x 0.9993 4.2277 129.31 105.19 122.92 Enhancement effect

[0126] Table 4 shows the results of the EC50 control of azoxystrobin against strawberry gray mold. 50 The concentration was 2.7456 mg / L; when the mass ratio of compound (I) to azoxystrobin was 1:25~24:1, it had a synergistic effect on gray mold in strawberries.

[0127] Table 4. Results of indoor combined effects of compound (I) and azoxystrobin on gray mold in strawberries.

[0128] Mass ratio Regression equation (y=) R <![CDATA[EC 50 (mg / L)]]> ATI TTI CTC interaction Compound A of formula (I) y = 3.9531 + 1.4191x 0.9987 5.4668 100 - - - Aminopyr B y = 4.3803 + 1.4191x 0.9985 2.7456 199.11 - - - 1:34 y = 4.4674 + 1.4357x 0.9986 2.3495 232.68 196.28 118.54 Additive effect 1:25 y = 4.5010 + 1.4277x 0.9991 2.2361 244.48 195.30 125.18 Enhancement effect 1:18 y = 4.5355 + 1.4091x 0.9944 2.1362 255.91 193.89 131.99 Enhancement effect 1:10 y = 4.5708 + 1.4340x 0.9976 1.9919 274.45 190.10 144.37 Enhancement effect 2:11 y = 4.6019 + 1.4065x 0.9981 1.9190 284.88 183.86 154.94 Enhancement effect 2:7 y = 4.6392 + 1.4346x 0.9987 1.7844 306.37 177.09 173.00 Enhancement effect 5:3 y = 4.5463 + 1.4642x 0.9995 2.0412 267.82 137.17 195.25 Enhancement effect 3:1 y = 4.4336 + 1.4243x 0.9971 2.4986 218.79 124.78 175.35 Enhancement effect 8:1 y = 4.3240 + 1.4199x 0.9983 2.9927 182.67 111.01 164.55 Enhancement effect 15:1 y = 4.2303 + 1.4265x 0.9982 3.4638 157.83 106.19 148.62 Enhancement effect 24:1 y = 4.1797 + 1.3634x 0.9987 3.9959 136.81 103.96 131.59 Enhancement effect

[0129] Example 2: Field efficacy trial

[0130] Experimental target: Strawberry gray mold

[0131] Experimental crop: Cream strawberry

[0132] Experimental location: Xiazhuang Strawberry Base, Chengyang District, Qingdao City. Greenhouses with a history of even occurrence and severe disease of strawberry gray mold were selected. The strawberry variety was Cream Strawberry, and the soil fertility was moderate. The strawberries grew uniformly.

[0133] Experimental Design: The experiment consisted of 10 treatments, each replicated 4 times, with water as a blank control. A completely randomized arrangement was used, with a plot area of ​​10 m². 2 Each community is randomly arranged into blocks, and adjacent communities are protected by a row.

[0134] Test reagents:

[0135] Table 5 Test reagents and dosages

[0136] deal with medicine <![CDATA[Active ingredient application rate (g a.i. / hm 2 )]]> C1 33% Formula (I) Compound·Benomylamide Water Dispersible Granules (24:9) 200 C2 30% Formula (I) Compound·Iprothiamethoxam Water Dispersible Granules (25:5) 200 C3 30% Formula (I) Compound·Bifenpyrazinamide Suspension Concentrate (24:6) 200 C4 32% Formula (I) Compound·Ampicillin Suspension Concentrate (20:12) 200 C5 20% Formula (I) Compound Suspension 200 C6 50% Cyclomethasone water-dispersible granules 225 C7 400g / L Isoprophiazone Suspension 300 C8 35% Bifenpyrazinamide Microemulsion 300 C9 35% Ambroxol Wettable Powder 300 C10 Water comparison /

[0137] Application time and frequency: Use a Huiteng 3WBS-20A backpack manual sprayer to spray the pesticide evenly, with about 750 mL of pesticide solution per area.

[0138] Apply pesticides twice, 7 days apart, during the early flowering and fruiting stage of strawberries, before the onset of diseases.

[0139] Survey method: The five-point survey method was adopted in each plot, with 50 fruits surveyed at each point. The damage of the compound to strawberries and the rate of diseased fruits were investigated 7 days after the first application and 10 days after the second application, according to the field trial standards for strawberry gray mold, and the control effect was calculated.

[0140] The results of the field efficacy trials are shown in the table below:

[0141] Table 6 shows the results of field efficacy trials. All tested agents showed varying degrees of inhibition against strawberry gray mold 7 days after the first application. Among them, the 33% compound (I)·pyridaben water-dispersible granules (24:9) had the best control efficacy of 75.2%.

[0142] Table 6. Field efficacy test results of different agents against strawberry gray mold (7 days after the first application)

[0143] deal with <![CDATA[Application rate of active ingredient (g a.i. / hm 2 )]]> Disease rate % Prevention and control efficacy % 33% Formula (I) Compound·Benomylamide Water Dispersible Granules (24:9) 200 8.20 75.20 30% Formula (I) Compound·Iprothiamethoxam Water Dispersible Granules (25:5) 200 9.00 73.03 30% Formula (I) Compound·Bifenpyrazinamide Suspension Concentrate (24:6) 200 9.90 69.91 32% Formula (I) Compound·Ampicillin Suspension Concentrate (20:12) 200 9.90 70.48 20% Formula (I) Compound Suspension 200 14.90 54.49 50% Cyclomethasone water-dispersible granules 225 12.60 62.07 400g / L Isoprophiazone Suspension 300 11.50 65.54 35% Bifenpyrazinamide Microemulsion 300 13.10 60.56 35% Ambroxol Wettable Powder 300 13.50 58.16 Blank control / 32.90 /

[0144] Table 7. Field efficacy test results of different agents against strawberry gray mold (10 days after the second application).

[0145] deal with <![CDATA[Active ingredient application rate (g a.i. / hm 2 )]]> Disease rate % Prevention and control efficacy % 33% Formula (I) Compound·Benomylamide Water Dispersible Granules (24:9) 200 4.80 90.25 30% Formula (I) Compound·Iprothiamethoxam Water Dispersible Granules (25:5) 200 5.80 88.33 30% Formula (I) Compound·Bifenpyrazinamide Suspension Concentrate (24:6) 200 7.20 85.31 32% Formula (I) Compound·Ampicillin Suspension Concentrate (20:12) 200 6.80 86.38 20% Formula (I) Compound Suspension 200 14.40 70.47 50% Cyclomethasone water-dispersible granules 225 11.70 76.35 400g / L Isoprophiazone Suspension 300 10.90 78.07 35% Bifenpyrazinamide Microemulsion 300 12.70 74.33 35% Ambroxol Wettable Powder 300 13.40 72.12 Blank control / 49.00 /

[0146] Analysis of the field efficacy test results in the table above shows that the control effect increased significantly 10 days after the second application. The control efficacies of 33% compound (I)•cyprodinil water-dispersible granules (24:9), 30% compound (I)•isoprothiamethoxam water-dispersible granules (25:5), 30% compound (I)•bifenpyrazinamide suspension (24:6), and 32% compound (I)•amypimidyl suspension (20:12) were 90.25%, 88.33%, 85.31%, and 86.38%, respectively, which were significantly higher than the control single agent.

[0147] In summary, through indoor toxicity testing and field efficacy trials, it can be seen that the fungicidal composition of the compound of formula (I) of this invention with boscalid, isothiazamide, bifenazate, and azoxystrobin has a good control effect on plant pathogens, is safe for target crops, and has significant efficacy. It is superior to single agents in delaying the development of resistance and prolonging the duration of efficacy, and can effectively reduce costs and pesticide residues.

[0148] Although this application describes specific embodiments in detail with the aid of examples, the disclosure of this application can be modified and substituted in various ways. However, it should be understood that the disclosure of this application is not limited to the specific form disclosed. Rather, the disclosure of this application covers all modifications, equivalents, and substitutions within the scope of the disclosure of this application, the scope of which is defined by the appended claims and their legal equivalents.

Claims

1. A bactericidal composition, characterized in that, The bactericidal composition comprises active ingredient A and active ingredient B, wherein active ingredient A is a compound of formula (I), with the following chemical structural formula: (I) The active ingredient B is any one of cyprodinil, isothiazinamide, bifenpyrazinamide, and azoxystrobin.

2. The bactericidal composition according to claim 1, characterized in that, The mass ratio of active ingredient A to active ingredient B is 1:25 to 32:

1.

3. The bactericidal composition according to claim 1, characterized in that, The mass ratio of the compound of formula (I) to cyazofamid is 1:18 to 18:1, the mass ratio of the compound of formula (I) to isothiazamide is 1:18 to 25:1, the mass ratio of the compound of formula (I) to bifenpyrazinamide is 1:18 to 32:1, and the mass ratio of the compound of formula (I) to azoxystrobin is 1:25 to 24:

1. Preferably, the mass ratio of the compound of formula (I) to cyazofamid is 1:18, 1:10, 2:11, 3:8, 2:1, 8:3, 11:2, 10:1, or 18:1; the mass ratio of the compound of formula (I) to isothiazamide is 1:18, 1:12, 1:8, 1:5, 5:4, 5:1, 10:1, 15:1, or 25:1; the mass ratio of the compound of formula (I) to bifenpyrazinamide is 1:18, 1:10, 1:6, 1:3, 1:1, 3:1, 6:1, 10:1, 18:1, or 32:1; and the mass ratio of the compound of formula (I) to azoxystrobin is 1:25, 1:18, 1:10, 2:11, 2:7, 5:3, 3:1, 8:1, 15:1, or 24:

1.

4. The bactericidal composition according to claim 1, characterized in that, The total weight of the bactericidal composition is 100 wt%, and the sum of the contents of active ingredient A and active ingredient B in the bactericidal composition is 1-90 wt%. Preferably, the total weight of the bactericidal composition is 100 wt%, and the sum of the contents of active ingredient A and active ingredient B in the bactericidal composition is 2 to 80 wt%.

5. The bactericidal composition according to claim 1, characterized in that, In addition to the active ingredients, the bactericidal composition also includes auxiliary ingredients.

6. The bactericidal composition according to claim 5, characterized in that, The auxiliary components include one or more of the following: wetting agents, dispersants, emulsifiers, thickeners, disintegrants, antifreeze agents, defoamers, solvents, stabilizers, film-forming agents, warning colors, penetrants, and carriers.

7. The bactericidal composition according to claim 1, characterized in that, The bactericidal composition can be prepared into any agriculturally permissible formulation, which may be a solid or liquid formulation.

8. A method for preventing or controlling plant pathogen infection, characterized in that, The bactericidal composition of claim 1 is used to prevent and control pathogens in agricultural, forestry or horticultural plants.

9. The method as described in claim 8, characterized in that, The plant pathogen mentioned is strawberry gray mold.

10. The method as described in claim 8, characterized in that, The bactericidal composition is applied in an effective amount to plant pathogens and / or their environment, or to plants, plant propagation material and subsequently grown plant organs, soil or cultivation medium, material or space.

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