Chinese herbal medicine compound preparation for preventing and controlling diarrhea of piglets, and preparation method and application thereof
By preparing compound preparations of traditional Chinese medicine with a particle size ≤180μm, the problem of poor solubility of traditional Chinese medicine preparations was solved, and more efficient treatment and prevention of diarrhea in piglets was achieved.
Patent Information
- Authority / Receiving Office
- CN · China
- Patent Type
- Applications(China)
- Current Assignee / Owner
- GUIZHOU UNIV
- Filing Date
- 2026-03-28
- Publication Date
- 2026-06-19
AI Technical Summary
Existing traditional Chinese medicine preparations for treating diarrhea in piglets suffer from poor solubility, leading to uneven dosage and reduced treatment and prevention efficiency.
A compound preparation of traditional Chinese medicines, including Coptis chinensis, Scutellaria baicalensis, Pulsatilla chinensis, Poria cocos, Paeonia lactiflora, Aucklandia lappa, Glycyrrhiza uralensis, Andrographis paniculata, Massa fermentata, nanoporous silica, β-cyclodextrin, phospholipids, and sodium butyrate, is extracted and concentrated into an extract by water extraction or alcohol extraction. After inclusion reaction, it is vacuum freeze-dried and low-temperature airflow pulverized to prepare a dry powder with a particle size ≤180μm, which is used for administration in drinking water or mixed with feed.
It improves the solubility and uniformity of the drug, enhances its efficacy, improves intestinal health, and significantly improves the treatment effect on piglet diarrhea.
Smart Images
Figure SMS_1 
Figure SMS_2 
Figure SMS_3
Abstract
Description
Technical Field
[0001] This invention relates to the field of traditional Chinese medicine technology, and in particular to a compound herbal preparation for preventing and treating diarrhea in piglets, its preparation method, and its application. Background Technology
[0002] Piglet diarrhea is a typical multifactorial disease under intensive pig farming conditions. It is one of the most serious piglet diseases and a major cause of piglet mortality. According to surveys, diarrhea accounts for 39.8% of all piglet deaths. Piglet diarrhea ranks first among the hazards in the pig farming industry, seriously threatening the healthy development of the industry. It leads to low feed conversion ratio, decreased piglet survival rate, slow growth, stunted growth and development, and even death. At present, antibiotics are mainly used to treat piglet diarrhea in clinical practice, but the effect is not ideal and there are also hazards such as drug residues.
[0003] Currently, using traditional Chinese medicine preparations to treat and prevent diarrhea in piglets is a common practice. These preparations are usually mixed into feed or drinking water. However, mixing them into feed increases the processing steps, while mixing them into drinking water results in uneven drug intake by piglets due to poor solubility, thus reducing the efficiency of treating and preventing diarrhea in piglets. Summary of the Invention
[0004] In view of this, the present invention provides a traditional Chinese medicine compound preparation for preventing and controlling diarrhea in piglets, comprising the following raw materials: Coptis chinensis, Scutellaria baicalensis, Pulsatilla chinensis, Poria cocos, Paeonia lactiflora, Aucklandia lappa, Glycyrrhiza uralensis, Andrographis paniculata, Massa fermentata, nanoporous silica, β-cyclodextrin, phospholipids, and sodium butyrate.
[0005] Preferably, the herbal compound preparation for preventing and controlling diarrhea in piglets comprises the following raw materials in parts by weight: 8-15 parts Coptis chinensis, 10-20 parts Scutellaria baicalensis, 10-20 parts Pulsatilla chinensis, 8-15 parts Poria cocos, 5-12 parts Paeonia lactiflora, 3-8 parts Aucklandia lappa, 3-10 parts Glycyrrhiza uralensis, 3-5 parts Andrographis paniculata, 2-5 parts Massa fermentata, 0.5-1 part nanoporous silica, 0.2-0.5 parts β-cyclodextrin, 0.1-0.4 parts phospholipids, and 0.1-0.3 parts sodium butyrate.
[0006] This invention also provides a method for preparing a traditional Chinese medicine compound preparation for preventing and controlling diarrhea in piglets, comprising the following steps: (1) Weigh out the Chinese herbal ingredients according to the proportion, and use water extraction or alcohol extraction to obtain Chinese herbal extract; (2) Concentrate the herbal extract obtained in step (1) to obtain an extract; (3) The extract is mixed with β-cyclodextrin, phospholipid and sodium butyrate, and stirred for 1-2 hours to carry out inclusion reaction. Then, nanoporous silica is added and mixed thoroughly to obtain a mixture. The mixture is then freeze-dried under vacuum and pulverized under low temperature to obtain the traditional Chinese medicine compound preparation for preventing and controlling piglet diarrhea.
[0007] Preferably, the extraction method of the traditional Chinese medicine extract in step (1) is as follows: Add Coptis chinensis to 5-8 times its volume of 60-75% ethanol solution and reflux at 70-80℃ twice, 1-2 hours each time. Combine the extracts to obtain Coptis chinensis extract. Mix Scutellaria baicalensis, Pulsatilla chinensis, Paeonia lactiflora, and Glycyrrhiza uralensis, add 10-15 times its volume of water and decoct 2-3 times, 1-2 hours each time. Combine the decoctions and filter to obtain the first aqueous extract. Mix Poria cocos, Andrographis paniculata, Massa fermentata, and Aucklandia lappa, soak in 6-10 times its volume of water for 0.5-1 hour, and extract twice at 90-100℃, 1.5-2.5 hours each time. Combine the extracts and filter to obtain the second aqueous extract. Finally, mix Coptis chinensis extract, the first aqueous extract, and the second aqueous extract to obtain the Chinese herbal extract.
[0008] Preferably, the relative density of the extract in step (2) at 60°C is 1.10-1.40.
[0009] Preferably, the temperature of the inclusion reaction in step (3) is 45-55℃.
[0010] Preferably, the water content of the mixture after vacuum freeze-drying in step (3) is ≤5%.
[0011] Preferably, the temperature of the air jet mill in step (3) is -10℃ to 0℃, the pressure is 1-2MPa, and the rotation speed is 1500-2000rpm; the particle size of the mixture after air jet milling is ≤180μm.
[0012] The present invention also provides the application of the traditional Chinese medicine compound preparation for preventing and controlling piglet diarrhea in the preparation of drugs for preventing and controlling piglet diarrhea.
[0013] Compared with the prior art, the present invention has the following beneficial effects: This invention provides a traditional Chinese medicine compound preparation for preventing and controlling diarrhea in piglets, its preparation method, and its application. The formula contains Coptis chinensis, Scutellaria baicalensis, and Pulsatilla chinensis to clear heat, dry dampness, detoxify, and stop diarrhea, treating heat-toxin-induced bloody dysentery; Poria cocos to strengthen the spleen and eliminate dampness; Paeonia lactiflora to nourish blood, astringe yin, relieve spasms, and stop pain; Aucklandia lappa to regulate qi and relieve pain; and Glycyrrhiza uralensis to harmonize the other herbs and enhance immunity. The combined effects of these herbs target the common pathogenesis of diarrhea in piglets, such as damp-heat, spleen deficiency, and qi stagnation, achieving a comprehensive effect of clearing heat, drying dampness, strengthening the spleen, stopping diarrhea, and regulating qi and blood. Furthermore, the separate extraction of different herbs enhances efficacy. Sodium butyrate can repair the intestinal mucosa and improve the gut microbiota; phospholipids aid in the absorption of active ingredients. Multiple components work synergistically to enhance the overall anti-diarrheal effect by repairing the intestinal barrier and regulating the gut microbiota. Furthermore, the use of β-cyclodextrin and phospholipids to encapsulate the extract protects volatile or easily oxidized components, masks bitterness, and improves water solubility. Nanoporous silica acts as an adsorbent carrier, loading the extract, increasing specific surface area, improving flowability, and contributing to enhanced solubility. This invention employs vacuum freeze-drying and low-temperature airflow pulverization to effectively prevent the decomposition and inactivation of heat-sensitive components during drying and pulverization, ensuring efficacy while maintaining a drug particle size ≤180μm. This significantly improves the drug's dissolution rate, dispersion uniformity, and contact area with the intestines. The resulting dried powder can be administered directly or diluted and mixed with feed or drinking water, making it suitable for group control in intensive livestock farms. Detailed Implementation
[0014] In a specific embodiment of the present invention, the nanoporous silica was purchased from Zhejiang Manli Nanotechnology Co., Ltd., with product specifications of 1~10um (mesoporous) and an effective substance content of 99.9%.
[0015] The present invention will be further described below with reference to the embodiments.
[0016] Example 1 A traditional Chinese medicine compound preparation for preventing and treating diarrhea in piglets is composed of the following raw materials in parts by weight: 10 parts Coptis chinensis, 13 parts Scutellaria baicalensis, 17 parts Pulsatilla chinensis, 10 parts Poria cocos, 10 parts Paeonia lactiflora, 5 parts Aucklandia lappa, 7 parts Glycyrrhiza uralensis, 4 parts Andrographis paniculata, 5 parts Massa fermentata, 0.6 parts nanoporous silica, 0.3 parts β-cyclodextrin, 0.3 parts phospholipids, and 0.2 parts sodium butyrate.
[0017] The preparation method of the herbal compound preparation for preventing and controlling diarrhea in piglets includes the following steps: (1) Weigh out the Chinese herbal ingredients according to the proportion, and use water extraction or alcohol extraction to obtain Chinese herbal extract; (2) The herbal extract obtained in step (1) is concentrated to obtain an extract with a relative density of 1.25 at 60℃; (3) The extract is mixed with β-cyclodextrin, phospholipid and sodium butyrate, and stirred at 50°C for 1-2 hours to carry out inclusion reaction. Then, nanoporous silica is added and mixed thoroughly to obtain a mixture. The mixture is then freeze-dried under vacuum and pulverized under low temperature to obtain the herbal compound preparation for preventing and controlling piglet diarrhea.
[0018] Preferably, the extraction method of the traditional Chinese medicine extract in step (1) is as follows: Coptis chinensis was added to 5 times its volume of 75% ethanol solution and refluxed twice at 75℃ for 2 hours each time. The extracts were combined to obtain the Coptis chinensis extract. Scutellaria baicalensis, Pulsatilla chinensis, Paeonia lactiflora, and Glycyrrhiza uralensis were mixed and decocted twice with 10 times its volume of water for 1.5 hours each time. The decoctions were combined and filtered to obtain the first aqueous extract. Poria cocos and Aucklandia lappa were mixed and soaked in 8 times its volume of water for 1 hour. Then, they were extracted twice at 95℃ for 1.5 hours each time. The extracts were combined and filtered to obtain the second aqueous extract. Finally, the Coptis chinensis extract, the first aqueous extract, and the second aqueous extract were mixed to obtain the traditional Chinese medicine extract. The vacuum freeze-drying and air jet milling were carried out using conventional methods. The water content of the mixture after vacuum freeze-drying was 4.7%. The air jet milling temperature was -5℃, the pressure was 1.0 MPa, and the rotation speed was 2000 rpm. The particle size of the mixture after air jet milling was ≤180 μm.
[0019] The resulting powder can be dispersed rapidly in water without clumping or becoming difficult to dissolve.
[0020] Comparative Example 1 A traditional Chinese medicine compound preparation for preventing and treating diarrhea in piglets, with the same raw material composition and preparation method as in Example 1, differing only in the extraction method of the herbal extract described in Comparative Example 1: Mix the Chinese herbs in a certain proportion, add them to 5 times the amount of 75% ethanol solution, and reflux extract twice at 75℃ for 2 hours each time. Combine the extracts to obtain the Chinese herbal extract.
[0021] The resulting powder can be dispersed rapidly in water without clumping or becoming difficult to dissolve.
[0022] Comparative Example 2 A traditional Chinese medicine compound preparation for preventing and treating diarrhea in piglets, with the same raw material composition and preparation method as in Example 1, differing only in the extraction method of the herbal extract described in Comparative Example 2: Mix the Chinese herbs in a certain proportion, soak them in 8 times the amount of water for 1 hour, and then extract them twice at 95℃ for 1.5 hours each time. Combine the extracts, filter them, and obtain the combined extract to obtain the Chinese herbal extract.
[0023] The resulting powder can be dispersed rapidly in water without clumping or becoming difficult to dissolve.
[0024] Comparative Example 3 A traditional Chinese medicine compound preparation for preventing and treating diarrhea in piglets, with the same raw material composition and preparation method as in Example 1, except that nanoporous silica is not added in Comparative Example 3.
[0025] The resulting powder partially agglomerates in water.
[0026] Comparative Example 4 A traditional Chinese medicine compound preparation for preventing and treating diarrhea in piglets, with the same raw material composition and preparation method as in Example 1, except that sodium butyrate is not added in Comparative Example 4.
[0027] The resulting powder partially agglomerates in water.
[0028] Comparative Example 5 A traditional Chinese medicine compound preparation for preventing and treating diarrhea in piglets, with the same raw material composition and preparation method as in Example 1, except that phospholipids are not added in Comparative Example 5.
[0029] Experiment 1: Two hundred and eighty healthy piglets aged 30 days were selected and divided into seven groups (each group was given the herbal compound preparation from Example 1 and Comparative Examples 1-6, respectively). The herbal compound preparations from Example 1 and Comparative Examples 1-6 were added to the drinking water at a dosage of 3g of raw medicine per pig. The administration was carried out once a day for seven consecutive days. The incidence of diarrhea in the piglets was recorded, and the results are shown in Table 1.
[0030] Table 1
[0031] After the experiment, the serum IgG level of piglets was measured, and the results are shown in Table 2.
[0032] Table 2
[0033] Experiment 2: Two hundred and eighty healthy piglets aged 30 days were selected and divided into seven groups (each group was given the herbal compound preparation from Example 1 and Comparative Examples 1-6, respectively). The herbal compound preparations from Example 1 and Comparative Examples 1-6 were added to the feed at a dosage of 3g of raw medicine per pig (the herbal compound preparations were mixed evenly with the feed) once a day for seven consecutive days. The incidence of diarrhea in the piglets was recorded, and the results are shown in Table 3.
[0034] Table 3
[0035] As can be seen from Tables 1 and 3, when administering the medication via feed, the efficacy of Example 1 and Comparative Examples 3-6 is not significantly different, indicating that the efficacy of the herbal compound preparations prepared with different additives is not significantly different. However, when administering the medication via drinking water, the efficacy of Example 1 is more significant, indicating that the herbal compound preparation of Example 1 has better dispersibility in water, which improves the convenience of administering the medication to piglets.
[0036] Experiment 3: 280 40-day-old piglets with diarrhea were selected and divided into 7 groups (each group was given the herbal compound preparation from Example 1 and Comparative Examples 1-6, respectively). The herbal compound preparations from Example 1 and Comparative Examples 1-6 were added to the drinking water at a dosage of 5g of raw medicine per pig. The administration was carried out once a day for 7 consecutive days. The cure rate of the piglets with diarrhea was recorded. The results are shown in Table 4.
[0037] Table 4
[0038] The above description is only a preferred embodiment of the present invention. It should be noted that for those skilled in the art, several improvements and modifications can be made without departing from the principle of the present invention, and these improvements and modifications should also be considered within the scope of protection of the present invention.
Claims
1. A traditional Chinese medicine compound preparation for preventing and treating diarrhea in piglets, characterized in that, The ingredients include: Coptis chinensis, Scutellaria baicalensis, Pulsatilla chinensis, Poria cocos, Paeonia lactiflora, Aucklandia lappa, Glycyrrhiza uralensis, Andrographis paniculata, Medicated Leaven, nanoporous silica, β-cyclodextrin, phospholipids, and sodium butyrate.
2. The traditional Chinese medicine compound preparation for preventing and controlling piglet diarrhea according to claim 1, characterized in that, The herbal compound preparation for preventing and treating diarrhea in piglets includes the following raw materials in parts by weight: 8-15 parts Coptis chinensis, 10-20 parts Scutellaria baicalensis, 10-20 parts Pulsatilla chinensis, 8-15 parts Poria cocos, 5-12 parts Paeonia lactiflora, 3-8 parts Aucklandia lappa, 3-10 parts Glycyrrhiza uralensis, 3-5 parts Andrographis paniculata, 2-5 parts Massa fermentata, 0.5-1 part nanoporous silica, 0.2-0.5 parts β-cyclodextrin, 0.1-0.4 parts phospholipids, and 0.1-0.3 parts sodium butyrate.
3. A method for preparing a traditional Chinese medicine compound preparation for preventing and controlling diarrhea in piglets as described in claim 1, characterized in that, Includes the following steps: (1) Weigh out the Chinese herbal ingredients according to the proportion, and use water extraction or alcohol extraction to obtain Chinese herbal extract; (2) Concentrate the herbal extract obtained in step (1) to obtain an extract; (3) The extract is mixed with β-cyclodextrin, phospholipid and sodium butyrate, and stirred for 1-2 hours to carry out inclusion reaction. Then, nanoporous silica is added and mixed thoroughly to obtain a mixture. The mixture is then freeze-dried under vacuum and pulverized under low temperature to obtain the traditional Chinese medicine compound preparation for preventing and controlling piglet diarrhea.
4. The method for preparing the traditional Chinese medicine compound preparation for preventing and controlling piglet diarrhea according to claim 3, characterized in that, The extraction method of the traditional Chinese medicine extract mentioned in step (1) is as follows: Add Coptis chinensis to 5-8 times its volume of 60-75% ethanol solution and reflux at 70-80℃ twice, 1-2 hours each time. Combine the extracts to obtain Coptis chinensis extract. Mix Scutellaria baicalensis, Pulsatilla chinensis, Paeonia lactiflora, and Glycyrrhiza uralensis, add 10-15 times its volume of water and decoct 2-3 times, 1-2 hours each time. Combine the decoctions and filter to obtain the first aqueous extract. Mix Poria cocos, Andrographis paniculata, Massa fermentata, and Aucklandia lappa, soak in 6-10 times its volume of water for 0.5-1 hour, and extract twice at 90-100℃, 1.5-2.5 hours each time. Combine the extracts and filter to obtain the second aqueous extract. Finally, mix Coptis chinensis extract, the first aqueous extract, and the second aqueous extract to obtain the Chinese herbal extract.
5. The method for preparing the traditional Chinese medicine compound preparation for preventing and controlling piglet diarrhea according to claim 3, characterized in that, The relative density of the extract in step (2) at 60°C is 1.10-1.
40.
6. The method for preparing the traditional Chinese medicine compound preparation for preventing and controlling piglet diarrhea according to claim 3, characterized in that, The temperature of the inclusion reaction in step (3) is 45-55℃.
7. The method for preparing the traditional Chinese medicine compound preparation for preventing and controlling piglet diarrhea according to claim 3, characterized in that, The water content of the mixture after vacuum freeze-drying in step (3) is ≤5%.
8. The method for preparing the traditional Chinese medicine compound preparation for preventing and controlling piglet diarrhea according to claim 3, characterized in that, The temperature of the air jet mill in step (3) is -10℃~0℃, the pressure is 1-2MPa, and the rotation speed is 1500-2000rpm; the particle size of the mixture after air jet milling is ≤180μm.
9. The use of the traditional Chinese medicine compound preparation for preventing and controlling piglet diarrhea as described in claim 1 in the preparation of a drug for preventing and controlling piglet diarrhea.